Adult female SD rats were ovariectomized and primed with esLradiol benzoate. Pituitarycytosol progesterone receptor was assayed by hydroxylapatite adsorption of
The effect of RU486, an antiprogestin, on luteal functions in the post-implantation mouse was studied. (1) In vivo experiment: Female adult mice were treated with RU486 at an oral dose of 100 mg/kg on day 1 and day 2 ...The effect of RU486, an antiprogestin, on luteal functions in the post-implantation mouse was studied. (1) In vivo experiment: Female adult mice were treated with RU486 at an oral dose of 100 mg/kg on day 1 and day 2 of pregnancy. Autopsy was展开更多
Intravaginal release of contraceptive steroid by avoiding the first-pass effect may provide a suitable route of administration, thus leading to higher bioavailabilities. RU486 is significantly metabolizlcd by the firs...Intravaginal release of contraceptive steroid by avoiding the first-pass effect may provide a suitable route of administration, thus leading to higher bioavailabilities. RU486 is significantly metabolizlcd by the first-pass metabolism. We have studied its展开更多
Pituitary cytosot was prepared from adult spayed Sprague-Dawtey female rats, primed by estradiol benzoate for 6 days. the progestin receptor binding activity of the pituitary cytosol was determined by hydroxylapatite ...Pituitary cytosot was prepared from adult spayed Sprague-Dawtey female rats, primed by estradiol benzoate for 6 days. the progestin receptor binding activity of the pituitary cytosol was determined by hydroxylapatite adsorption of the bound steroid-receptor complex with tritiated R5020 as ligund. Seatchard analysis of saturation curve showed that the dissociation constant (Kd) of the bound progestin(P) receptor ligand complex was 1.8nM and the greatest binding site was 138 fmoles/mg protein. RU486 could competitively inhibB the binding of ^3H-R5020 to pituitary cytosol progestin receptor.The competitve ability of the untabelled RU486, R5020 or progesterone with ^3H-R5020 are essentially the same.展开更多
文摘Adult female SD rats were ovariectomized and primed with esLradiol benzoate. Pituitarycytosol progesterone receptor was assayed by hydroxylapatite adsorption of
文摘The effect of RU486, an antiprogestin, on luteal functions in the post-implantation mouse was studied. (1) In vivo experiment: Female adult mice were treated with RU486 at an oral dose of 100 mg/kg on day 1 and day 2 of pregnancy. Autopsy was
文摘Intravaginal release of contraceptive steroid by avoiding the first-pass effect may provide a suitable route of administration, thus leading to higher bioavailabilities. RU486 is significantly metabolizlcd by the first-pass metabolism. We have studied its
文摘Pituitary cytosot was prepared from adult spayed Sprague-Dawtey female rats, primed by estradiol benzoate for 6 days. the progestin receptor binding activity of the pituitary cytosol was determined by hydroxylapatite adsorption of the bound steroid-receptor complex with tritiated R5020 as ligund. Seatchard analysis of saturation curve showed that the dissociation constant (Kd) of the bound progestin(P) receptor ligand complex was 1.8nM and the greatest binding site was 138 fmoles/mg protein. RU486 could competitively inhibB the binding of ^3H-R5020 to pituitary cytosol progestin receptor.The competitve ability of the untabelled RU486, R5020 or progesterone with ^3H-R5020 are essentially the same.
