Objective： Being antigen-presenting cells, dendritic cells （DCs） transport captured antigen from peripheral tissues to T cell zone of lymph nodes via lymphatic vessels. This migration is essential for the presentat...Objective： Being antigen-presenting cells, dendritic cells （DCs） transport captured antigen from peripheral tissues to T cell zone of lymph nodes via lymphatic vessels. This migration is essential for the presentation of antigen that leads to priming of effector T cell responses. In this study, we tried to promote the migratory ability of mouse bone marrow-derived dendritic cells （BMDCs） loaded with antigen of breast cancer, and its immunological effect in vivo. Methods： After being loaded with breast carcinoma antigen, BMDCs were cultured with medium containing PGE2, LTC4, or Bryo-1 respectively. Phenotypic changes, CCR7 expression, chemotaxis assay, mixed lymphocyte response, specific T lymphocyte cytotoxicity assay and anti-tumor immune efficacy of BMDCs were observed. Results ： PGF2 and LTC4 promoted maturation, CCR7 expression and migratory ability of BMDCs compared with control group in vitro. In vivo PGE2 and LTC4 group vaccines were more efficient on suppressing growth of mouse breast cancer than other groups. However Bryo-1 only enhanced BMDCs maturation. Conclusion： Because the effect of specific CTL in vitro had no difference, we suggested that migration of dendritic cells to lymph nodes maybe answered for the better anti-tumor immunological response induced by PGE2 or LTC4 in vivo.展开更多
Cancer Rehabilitation is a new discipline,a combination of tumor medication,immunology,psychology,nutritional science and exercise physiology etc..The core of cancer rehabilitation is the therapy of natural immunology...Cancer Rehabilitation is a new discipline,a combination of tumor medication,immunology,psychology,nutritional science and exercise physiology etc..The core of cancer rehabilitation is the therapy of natural immunology,which aims at activating T-cells,and restoring the bone marrow function impaired during chemo-radiation therapy.Cancer rehabilitation seeks to achieve the gradual recovery of the immune system,which in turn hinders recurrence and metastasis.In addition,with the help of psychological consultation,nutritional and physical exercise guidance,cancer patients may have a better chance at managing the risk of recurrence and metastasis,extending life expectancy with an improved quality of life.展开更多
文摘Objective： Being antigen-presenting cells, dendritic cells （DCs） transport captured antigen from peripheral tissues to T cell zone of lymph nodes via lymphatic vessels. This migration is essential for the presentation of antigen that leads to priming of effector T cell responses. In this study, we tried to promote the migratory ability of mouse bone marrow-derived dendritic cells （BMDCs） loaded with antigen of breast cancer, and its immunological effect in vivo. Methods： After being loaded with breast carcinoma antigen, BMDCs were cultured with medium containing PGE2, LTC4, or Bryo-1 respectively. Phenotypic changes, CCR7 expression, chemotaxis assay, mixed lymphocyte response, specific T lymphocyte cytotoxicity assay and anti-tumor immune efficacy of BMDCs were observed. Results ： PGF2 and LTC4 promoted maturation, CCR7 expression and migratory ability of BMDCs compared with control group in vitro. In vivo PGE2 and LTC4 group vaccines were more efficient on suppressing growth of mouse breast cancer than other groups. However Bryo-1 only enhanced BMDCs maturation. Conclusion： Because the effect of specific CTL in vitro had no difference, we suggested that migration of dendritic cells to lymph nodes maybe answered for the better anti-tumor immunological response induced by PGE2 or LTC4 in vivo.
文摘Cancer Rehabilitation is a new discipline,a combination of tumor medication,immunology,psychology,nutritional science and exercise physiology etc..The core of cancer rehabilitation is the therapy of natural immunology,which aims at activating T-cells,and restoring the bone marrow function impaired during chemo-radiation therapy.Cancer rehabilitation seeks to achieve the gradual recovery of the immune system,which in turn hinders recurrence and metastasis.In addition,with the help of psychological consultation,nutritional and physical exercise guidance,cancer patients may have a better chance at managing the risk of recurrence and metastasis,extending life expectancy with an improved quality of life.
