The aim of this paper is to investigate the safety of reconstructed adenovirus in immunosuppressive ther-apeutics and to explore the role of ciclosporin A in ant-agonizing the elimination of the vector.Several rats we...The aim of this paper is to investigate the safety of reconstructed adenovirus in immunosuppressive ther-apeutics and to explore the role of ciclosporin A in ant-agonizing the elimination of the vector.Several rats were given retroperitoneal injection of purified ADV-TK in order to obtain models.After 14 days’treatment of ciclos-porin A,samples of different periods were obtained,then stained with hematoxylin-eosin(HE)to detect inflam-mation reactions.Immunohistochemistry was used to examine the expression of adenovirus in organs.The results are as follows:(1)In HE stained sections of the organs,some transitory and reversible inflammation was detected.(2)In immunohistochemistry assay,recon-structed adenovirus decreased gradually as time went by in the control group,while it did not happen in the experi-mental group in which the adenovirus showed a relative increase compared with their counterparts(P,0.05).(3)The distributions of adenovirus in the liver,spleen and lung were higher than those in the other organs detected.Reconstructed adenovirus as a vector is definitely safe in immunosuppressive therapeutics,and ciclosporin A,to some extent,is able to consequently inhibit the immune response of the rats and prolong the existing period of adenovirus.展开更多
基金supported by National Key Basic Research Foundation of China(No.2002CB513107)the National Natural Science Foundation of China(Grant No.C03020705).
文摘The aim of this paper is to investigate the safety of reconstructed adenovirus in immunosuppressive ther-apeutics and to explore the role of ciclosporin A in ant-agonizing the elimination of the vector.Several rats were given retroperitoneal injection of purified ADV-TK in order to obtain models.After 14 days’treatment of ciclos-porin A,samples of different periods were obtained,then stained with hematoxylin-eosin(HE)to detect inflam-mation reactions.Immunohistochemistry was used to examine the expression of adenovirus in organs.The results are as follows:(1)In HE stained sections of the organs,some transitory and reversible inflammation was detected.(2)In immunohistochemistry assay,recon-structed adenovirus decreased gradually as time went by in the control group,while it did not happen in the experi-mental group in which the adenovirus showed a relative increase compared with their counterparts(P,0.05).(3)The distributions of adenovirus in the liver,spleen and lung were higher than those in the other organs detected.Reconstructed adenovirus as a vector is definitely safe in immunosuppressive therapeutics,and ciclosporin A,to some extent,is able to consequently inhibit the immune response of the rats and prolong the existing period of adenovirus.
