Objective:To examine the effects of modified Shenmai Yin on invigorating vital energy, promoting blood flow, and protection against neural impairment in an endotoxin-induced shock rat model. Methods: Ninety-six SD rat...Objective:To examine the effects of modified Shenmai Yin on invigorating vital energy, promoting blood flow, and protection against neural impairment in an endotoxin-induced shock rat model. Methods: Ninety-six SD rats were randomly divided into four groups: sham operation (saline 20 ml/kg), shock model (lipopolysaccharide, LPS, 8 mg/kg), Reformed Shengmai Yin (加味生脉饮 Pulse-activating Decoction) (LPS 8 mg/kg + reformed Shengmai Yin Injection 10 ml/kg), and dexamethasone (LPS 8 mg/kg + dexamethasone 5 mg/kg) groups. Each group was subdivided into 1 h, 2 h, 3 h, and 6 h time points for observation. The carotid artery was separated and connected with a biological functional system to monitor mean arterial pressure (MAP). Brain water levels, malonaldehyde (MDA) content, and superoxide dismutase (SOD) activity were also determined. Results: In the shock model group, MAP was progressively decreased after injection of LPS, brain water and MDA contents were increased, brain SOD activity was decreased, and capillary vessel edema in brain tissue was also observed. All these parameters were improved significantly in both treatment groups, although the effects were more marked with Shengmai Yin than with dexamethasone. Conclusion: Modified Shengmai Yin exhibits strong anti-shock and neuroprotective effects against Endotoxininduced shock.展开更多
Acute Yin impairment is a frequently occurring clinic syndrome. We suggest the process of acute Yin impairment be divided into two stages, namely early impairment of lung and stomach fluid and later Yin impairment of ...Acute Yin impairment is a frequently occurring clinic syndrome. We suggest the process of acute Yin impairment be divided into two stages, namely early impairment of lung and stomach fluid and later Yin impairment of liver and kidney. This work aims to research into the cell protecting mechanism of Zengyetang, from the angle of protecting cells, change of inner and outer fluid ion of cells, change of enzyme of cell membrane, free radical impairment, appoptosis and gene expression. The study, taking inner and outer fluid ion of cells as objective indicators, also researches into dynamic change of inner and outer fluid of cells in the different stages of Yin impairment development on the animal models of yin impairment by fever and poison. Zengyetang is one of the typical Yin nourishing prescriptions. Zengyetang is made into injection in accordance with the rate of the original prescription. This work researches into the affects of Zengyetang on the four types of acute Yin impairment animal models, including Yin impairment model by pathogenic heat, Yin impairment model by summer heat and sweat, Yin impairment model by high infiltration Yin impairment and Yin impairment by drugs and toxin. Main experiment results show:1. in early stage, pathogenic heat Yin impairment impairs outer liquid of cells; 2. Zengyetang has the function of adjusting disorder of inner and outer fluid of red cells; 3. Zengyetang has the function of protecting enzyme activity of cell membrane and maintaining normal operation of red cell ion; 4. Zengyetang can protect liver cells of rabbits through resisting free radical impairment and lessen thymocyte apoptosis of young rat resulted from glucocorticoid.展开更多
目的:观察伏格列波糖分散片(家能)联合五参口服液干预治疗糖耐量低减(IGT)的转归、症状变化及其不良反应,以探求糖尿病(DM)二级预防的最佳方式方法。方法:选择IGT 60例随机分为家能组、家能加五参口服液组(五参联合组)及安慰剂组,3组均...目的:观察伏格列波糖分散片(家能)联合五参口服液干预治疗糖耐量低减(IGT)的转归、症状变化及其不良反应,以探求糖尿病(DM)二级预防的最佳方式方法。方法:选择IGT 60例随机分为家能组、家能加五参口服液组(五参联合组)及安慰剂组,3组均在糖尿病饮食、运动疗法的基础上,连续服药治疗8周。治疗前后所有患者均检测空腹血糖(FPG)、口服75 g OGTT后半小时、2小时的血糖(2 h PG)、糖化血红蛋白和空腹胰岛素分泌水平(FINS)及糖负荷后半小时胰岛素水平,并计算3组治疗前后胰岛素敏感性指数(ISI)、早期胰岛素分泌指数(△Ins30/△Glu30)、胰岛素抵抗指数(HOMA-IR)、胰岛B细胞功能指数(HOMA-P),检测其服药后血常规、肝功能、血脂、肾功能、体质量指数(BMI)及其血压变化,并记录用药期间不良反应情况。结果:治疗后,家能组及家能加五参组2 h PG较治疗前明显下降(P<0.05),ISI、HOMA-IR、HOMA-、△Ins30/△Glu30明显改善(P<0.05),与安慰剂组比较改善明显(P<0.05)。体质量指数家能加五参组较家能组及安慰剂组下降明显(P<0.05)。家能组、家能加五参组两组糖耐量转为正常比率分别为97.6%、98%。血常规、血脂、肝功能、肾功能及血压各组治疗前后无明显变化(P>0.05)。家能加五参组症状较家能组及安慰剂组改善明显,不良反应腹胀、腹泻、腹痛、便秘及低血糖、皮疹发生率明显低于家能组及安慰组(P<0.05)。结论:伏格列波糖分散片能明显增加IGT向正常糖耐量(NGT)的转化率,明显降低IGT餐后血糖、增加早期胰岛素分泌,改善胰岛素抵抗及胰岛B细胞功能,联合五参口服液治疗可明显改善IGT症状,增强上述作用,减少不良反应的发生。展开更多
基金supported by a grant from National Natural Science Foundation of China (No.30672737)
文摘Objective:To examine the effects of modified Shenmai Yin on invigorating vital energy, promoting blood flow, and protection against neural impairment in an endotoxin-induced shock rat model. Methods: Ninety-six SD rats were randomly divided into four groups: sham operation (saline 20 ml/kg), shock model (lipopolysaccharide, LPS, 8 mg/kg), Reformed Shengmai Yin (加味生脉饮 Pulse-activating Decoction) (LPS 8 mg/kg + reformed Shengmai Yin Injection 10 ml/kg), and dexamethasone (LPS 8 mg/kg + dexamethasone 5 mg/kg) groups. Each group was subdivided into 1 h, 2 h, 3 h, and 6 h time points for observation. The carotid artery was separated and connected with a biological functional system to monitor mean arterial pressure (MAP). Brain water levels, malonaldehyde (MDA) content, and superoxide dismutase (SOD) activity were also determined. Results: In the shock model group, MAP was progressively decreased after injection of LPS, brain water and MDA contents were increased, brain SOD activity was decreased, and capillary vessel edema in brain tissue was also observed. All these parameters were improved significantly in both treatment groups, although the effects were more marked with Shengmai Yin than with dexamethasone. Conclusion: Modified Shengmai Yin exhibits strong anti-shock and neuroprotective effects against Endotoxininduced shock.
文摘Acute Yin impairment is a frequently occurring clinic syndrome. We suggest the process of acute Yin impairment be divided into two stages, namely early impairment of lung and stomach fluid and later Yin impairment of liver and kidney. This work aims to research into the cell protecting mechanism of Zengyetang, from the angle of protecting cells, change of inner and outer fluid ion of cells, change of enzyme of cell membrane, free radical impairment, appoptosis and gene expression. The study, taking inner and outer fluid ion of cells as objective indicators, also researches into dynamic change of inner and outer fluid of cells in the different stages of Yin impairment development on the animal models of yin impairment by fever and poison. Zengyetang is one of the typical Yin nourishing prescriptions. Zengyetang is made into injection in accordance with the rate of the original prescription. This work researches into the affects of Zengyetang on the four types of acute Yin impairment animal models, including Yin impairment model by pathogenic heat, Yin impairment model by summer heat and sweat, Yin impairment model by high infiltration Yin impairment and Yin impairment by drugs and toxin. Main experiment results show:1. in early stage, pathogenic heat Yin impairment impairs outer liquid of cells; 2. Zengyetang has the function of adjusting disorder of inner and outer fluid of red cells; 3. Zengyetang has the function of protecting enzyme activity of cell membrane and maintaining normal operation of red cell ion; 4. Zengyetang can protect liver cells of rabbits through resisting free radical impairment and lessen thymocyte apoptosis of young rat resulted from glucocorticoid.
文摘目的:观察伏格列波糖分散片(家能)联合五参口服液干预治疗糖耐量低减(IGT)的转归、症状变化及其不良反应,以探求糖尿病(DM)二级预防的最佳方式方法。方法:选择IGT 60例随机分为家能组、家能加五参口服液组(五参联合组)及安慰剂组,3组均在糖尿病饮食、运动疗法的基础上,连续服药治疗8周。治疗前后所有患者均检测空腹血糖(FPG)、口服75 g OGTT后半小时、2小时的血糖(2 h PG)、糖化血红蛋白和空腹胰岛素分泌水平(FINS)及糖负荷后半小时胰岛素水平,并计算3组治疗前后胰岛素敏感性指数(ISI)、早期胰岛素分泌指数(△Ins30/△Glu30)、胰岛素抵抗指数(HOMA-IR)、胰岛B细胞功能指数(HOMA-P),检测其服药后血常规、肝功能、血脂、肾功能、体质量指数(BMI)及其血压变化,并记录用药期间不良反应情况。结果:治疗后,家能组及家能加五参组2 h PG较治疗前明显下降(P<0.05),ISI、HOMA-IR、HOMA-、△Ins30/△Glu30明显改善(P<0.05),与安慰剂组比较改善明显(P<0.05)。体质量指数家能加五参组较家能组及安慰剂组下降明显(P<0.05)。家能组、家能加五参组两组糖耐量转为正常比率分别为97.6%、98%。血常规、血脂、肝功能、肾功能及血压各组治疗前后无明显变化(P>0.05)。家能加五参组症状较家能组及安慰剂组改善明显,不良反应腹胀、腹泻、腹痛、便秘及低血糖、皮疹发生率明显低于家能组及安慰组(P<0.05)。结论:伏格列波糖分散片能明显增加IGT向正常糖耐量(NGT)的转化率,明显降低IGT餐后血糖、增加早期胰岛素分泌,改善胰岛素抵抗及胰岛B细胞功能,联合五参口服液治疗可明显改善IGT症状,增强上述作用,减少不良反应的发生。
