Two new tetrahydronaphthalenes,lα,2α-diol-4α-ethoxy-1,2,3,4-tetrahydronaphthalene(1) and 1a,2a,4β-triol-1,2,3,4- tetrahydronaphthalene(2),were isolated from the stem of Impatiens balsamina L.Their structures were ...Two new tetrahydronaphthalenes,lα,2α-diol-4α-ethoxy-1,2,3,4-tetrahydronaphthalene(1) and 1a,2a,4β-triol-1,2,3,4- tetrahydronaphthalene(2),were isolated from the stem of Impatiens balsamina L.Their structures were elucidated by various spectroscopic methods.展开更多
[Objectives]This study was conducted to investigate the extraction method of Impatiens balsamina L.(Tougucao),and to optimize the extraction process conditions and parameters.[Methods]Different ethanol concentrations,...[Objectives]This study was conducted to investigate the extraction method of Impatiens balsamina L.(Tougucao),and to optimize the extraction process conditions and parameters.[Methods]Different ethanol concentrations,ethanol amounts and extraction time were investigated by an orthogonal test L_(9)(3^(4))and high performance liquid chromatography(HPLC),and the extraction process parameters were evaluated by the yield of extract and the content of quercetin.[Results]Under the three optimal extraction conditions of impregnation,heating reflux and ultrasound,the extract yield and quercetin contents of I.balsamina were 8.27%,9.50%,7.09%and 1.60,3.05,3.03μg/g,respectively.Heating reflux extraction and ultrasonic extraction showed higher extract yields and quercetin contents of I.balsamina,while the values were lower under the impregnation extraction conditions.The primary and secondary factors were:ethanol amount(C)>ethanol concentration(A)>extraction time(B).The optimal extraction process of I.balsamina was as follows:adding 10 times of 60%ethanol and ultrasonically extracting for 30 min.[Conclusions]The method is simple,convenient,accurate and reliable,and provides a scientific basis for the research on the extraction process of I.balsamina and its preparations.展开更多
Objective:To assess the larvicidal potential of the crude benzene,chloroform,ethyl acetate and methanol solvent extracts of the medicinal plant Impatiens balsamina against Anopheles stephensi(An.stephensi),Aedes aegyp...Objective:To assess the larvicidal potential of the crude benzene,chloroform,ethyl acetate and methanol solvent extracts of the medicinal plant Impatiens balsamina against Anopheles stephensi(An.stephensi),Aedes aegypti(Ae.aegypti)and Culex quinquefasciatus(Cx.quinquefasciatus).Methods:Twenty five third instar larvae of An.stephensi,Ae.aegypti and Cx.quinquefasciatus were exposed to various concentrations and were assayed in the laboratory by using the protocol recommended by WHO.The larval mortality was observed after 24 h of treatment.Results:Among extracts tested,the highest larvicidal activity was observed in leaf methanol extract of Impatiens balsamina against An.stephensi,Ae.aegypti and Cx.quinquefasciatus with the LC_(50)and LC_(90)values 98.04,119.68,125.06 and 172.93,210.14,220.60 mg/L,respectively.Conclusions:From the results it can be concluded that the larvicidal effect of Impatiens balsamina against An.stephensi,Ae.aegypti and Cx.quinquefasciatus make this plant product promising as an alternative to synthetic insecticide in mosquito control programs.展开更多
A simple and efficient HPLC method was developed for quality analysis of flavonoids from Impatiens balsamina L.(IBL)flowers,which were collected from Xingjiang,Anhui,Henan,and Hubei provinces in China.The flavonoids s...A simple and efficient HPLC method was developed for quality analysis of flavonoids from Impatiens balsamina L.(IBL)flowers,which were collected from Xingjiang,Anhui,Henan,and Hubei provinces in China.The flavonoids substances in IBL were determined by HPLC through methyl alcohol ultrasonic extraction.A mixture of展开更多
AMPs （antimicrobial peptides） are small, mostly basic peptides that range in size from 2-9 kDa, and they are an important component of the innate defense system of plants where they are effector molecules considered...AMPs （antimicrobial peptides） are small, mostly basic peptides that range in size from 2-9 kDa, and they are an important component of the innate defense system of plants where they are effector molecules considered to be an important defense barrier to pathogens and pests. Nine families of antimicrobial peptides have been identified in plants, including thionins, defensins, lipid transfer proteins, hevein and knotting-like peptides, four cysteine-types, and the recently reported shepherdins, snakins and cyclotides. They are part of both permanent and inducible defense barriers of plants. Transgenic overexpression of the corresponding genes leads to enhanced tolerance to pathogens, and peptide-sensitive pathogen mutants have reduced virulence. In this review, the recent studies on peptides from plant sources, including peptides isolated from indigenous medicine and edible plants of Central-Asia, are briefly discussed with a focus on their origins, antioxidant, antitumor activities and the possible mechanisms of actions in order to provide a profile of important plant peptides.展开更多
文摘Two new tetrahydronaphthalenes,lα,2α-diol-4α-ethoxy-1,2,3,4-tetrahydronaphthalene(1) and 1a,2a,4β-triol-1,2,3,4- tetrahydronaphthalene(2),were isolated from the stem of Impatiens balsamina L.Their structures were elucidated by various spectroscopic methods.
基金Supported by Scientific Research Project of Guizhou University of Traditional Chinese Medicine([2019]64)。
文摘[Objectives]This study was conducted to investigate the extraction method of Impatiens balsamina L.(Tougucao),and to optimize the extraction process conditions and parameters.[Methods]Different ethanol concentrations,ethanol amounts and extraction time were investigated by an orthogonal test L_(9)(3^(4))and high performance liquid chromatography(HPLC),and the extraction process parameters were evaluated by the yield of extract and the content of quercetin.[Results]Under the three optimal extraction conditions of impregnation,heating reflux and ultrasound,the extract yield and quercetin contents of I.balsamina were 8.27%,9.50%,7.09%and 1.60,3.05,3.03μg/g,respectively.Heating reflux extraction and ultrasonic extraction showed higher extract yields and quercetin contents of I.balsamina,while the values were lower under the impregnation extraction conditions.The primary and secondary factors were:ethanol amount(C)>ethanol concentration(A)>extraction time(B).The optimal extraction process of I.balsamina was as follows:adding 10 times of 60%ethanol and ultrasonically extracting for 30 min.[Conclusions]The method is simple,convenient,accurate and reliable,and provides a scientific basis for the research on the extraction process of I.balsamina and its preparations.
文摘Objective:To assess the larvicidal potential of the crude benzene,chloroform,ethyl acetate and methanol solvent extracts of the medicinal plant Impatiens balsamina against Anopheles stephensi(An.stephensi),Aedes aegypti(Ae.aegypti)and Culex quinquefasciatus(Cx.quinquefasciatus).Methods:Twenty five third instar larvae of An.stephensi,Ae.aegypti and Cx.quinquefasciatus were exposed to various concentrations and were assayed in the laboratory by using the protocol recommended by WHO.The larval mortality was observed after 24 h of treatment.Results:Among extracts tested,the highest larvicidal activity was observed in leaf methanol extract of Impatiens balsamina against An.stephensi,Ae.aegypti and Cx.quinquefasciatus with the LC_(50)and LC_(90)values 98.04,119.68,125.06 and 172.93,210.14,220.60 mg/L,respectively.Conclusions:From the results it can be concluded that the larvicidal effect of Impatiens balsamina against An.stephensi,Ae.aegypti and Cx.quinquefasciatus make this plant product promising as an alternative to synthetic insecticide in mosquito control programs.
文摘A simple and efficient HPLC method was developed for quality analysis of flavonoids from Impatiens balsamina L.(IBL)flowers,which were collected from Xingjiang,Anhui,Henan,and Hubei provinces in China.The flavonoids substances in IBL were determined by HPLC through methyl alcohol ultrasonic extraction.A mixture of
文摘AMPs （antimicrobial peptides） are small, mostly basic peptides that range in size from 2-9 kDa, and they are an important component of the innate defense system of plants where they are effector molecules considered to be an important defense barrier to pathogens and pests. Nine families of antimicrobial peptides have been identified in plants, including thionins, defensins, lipid transfer proteins, hevein and knotting-like peptides, four cysteine-types, and the recently reported shepherdins, snakins and cyclotides. They are part of both permanent and inducible defense barriers of plants. Transgenic overexpression of the corresponding genes leads to enhanced tolerance to pathogens, and peptide-sensitive pathogen mutants have reduced virulence. In this review, the recent studies on peptides from plant sources, including peptides isolated from indigenous medicine and edible plants of Central-Asia, are briefly discussed with a focus on their origins, antioxidant, antitumor activities and the possible mechanisms of actions in order to provide a profile of important plant peptides.
