基于U-ISP的UF32 Flash编程方法

在进行飞思卡尔UF32 Flash存储器用户模式在线编程时,发现Flash阵列上的高电压导致其在读取时发生不稳定的现象,提出一种解决方法。该方法遵循嵌入式软件工程的模块化思想与封装原则,设计通用的擦除与写入子函数。实例证明该方法解决了Flash存储器在线编程时的稳定性问题,且通用性强、编程方便。

着床丝氨酸蛋白酶-2(ISP2)蛋白在小鼠卵子和早胚组织中的表达及其定位

目的:了解ISP2蛋白在小鼠卵细胞和早胚组织中的表达情况。方法:收集小鼠未成熟卵、成熟卵、受精卵和着床前胚泡,利用特异性抗ISP2抗体,采用免疫荧光和激光共聚焦显微镜技术对ISP2在卵细胞和早期胚胎组织中的表达模式以及亚细胞定位进行检测分析。结果:在小鼠未受精卵、受精卵和着床前胚泡中均能检测到特异的ISP2蛋白信号;通过激光共聚焦显微镜观察到ISP2在细胞膜上的信号强于其在细胞质。结论:ISP2蛋白在小鼠卵子和早胚组织中均有表达,并主要分布在细胞膜上。

教学实验用AT89S51-ISP编程器的设计

目前多数电子信息类院校仍沿用51核单片机作为基本实验装置,其中以AT89S51单片机最为普及。虽然企业版编程器支持的单片机品种众多、功能庞大,但学校的单片机教学实验并不需要支持多品种单片机,只支持AT89S51单一芯片,且只以编程为目的的单片机编程器更适合学校教学实验用。文中设计的教学实验用AT89S51-ISP编程器采用USB接口、ISP技术、只支持AT89S51/52,以能编程为唯一目标。

“通知-删除”规则在ISP适用的困境与出路

“通知-删除”规则平衡著作权人、网络服务提供者、网络用户三方利益,是ISP应遵守的法定义务。我国“通知-删除”规则存在ISP界定和分类不完善、合格通知性质不明晰、合格通知要件不准确、不合格通知后果不明确、不重视转通知程序和反通知程序等问题,存在ISP于网络服务协议中直接规定“不通知用户即可删除资料”的现象。因此,应完善ISP主体方面的规定;增加通知实质性符合要件时视为合格通知的规定;完善合格通知要件的表述;鼓励ISP在能力范围内自主创新,采取事前措施以减少不合格通知;修改转通知程序、反通知程序方面的规定。应注重平衡三方利益,不忽视用户利益,让ISP真正中立,促进网络环境的繁荣与发展。

Pivotal role of long non-coding ribonucleic acid-X-inactive specific transcript in regulating immune checkpoint programmed death ligand 1 through a shared pathway between miR-194-5p and miR-155-5p in hepatocellular carcinoma

BACKGROUND Anti-programmed death therapy has thrust immunotherapy into the spotlight.However,such therapy has a modest response in hepatocellular carcinoma(HCC).Epigenetic immunomodulation is a suggestive combinatorial therapy with immune checkpoint blockade.Non-coding ribonucleic acid(ncRNA)driven regulation is a major mechanism of epigenetic modulation.Given the wide range of ncRNAs that co-opt in programmed cell-death protein 1(PD-1)/programmed death ligand 1(PD-L1)regulation,and based on the literature,we hypothesized that miR-155-5p,miR-194-5p and long non-coding RNAs(lncRNAs)X-inactive specific transcript(XIST)and MALAT-1 are involved in a regulatory upstream pathway for PD-1/PD-L1.Recently,nutraceutical therapeutics in cancers have received increasing attention.Thus,it is interesting to study the impact of oleuropein on the respective study key players.AIM To explore potential upstream regulatory ncRNAs for the immune checkpoint PD-1/PD-L1.METHODS Bioinformatics tools including microrna.org and lnCeDB software were adopted to detect targeting of miR-155-5p,miR-194-5p and lncRNAs XIST and MALAT-1 to PD-L1 mRNA,respectively.In addition,Diana tool was used to predict targeting of both aforementioned miRNAs to lncRNAs XIST and MALAT-1.HCC and normal tissue samples were collected for scanning of PD-L1,XIST and MALAT-1 expression.To study the interaction among miR-155-5p,miR-194-5p,lncRNAs XIST and MALAT-1,as well as PD-L1 mRNA,a series of transfections of the Huh-7 cell line was carried out.RESULTS Bioinformatics software predicted that miR-155-5p and miR-194-5p can target PDL1,MALAT-1 and XIST.MALAT-1 and XIST were predicted to target PD-L1 mRNA.PD-L1 and XIST were significantly upregulated in 23 HCC biopsies compared to healthy controls;however,MALAT-1 was barely detected.MiR-194 induced expression elevated the expression of PD-L1,XIST and MALAT-1.However,overexpression of miR-155-5p induced the upregulation of PD-L1 and XIST,while it had a negative impact on MALAT-1 expression.Knockdown of XIST did have an impact on PD-L1 expression;however,following knockdown of the negative regulator of X-inactive specific transcript(TSIX),PD-L1 expression was elevated,and abolished MALAT-1 activity.Upon co-transfection of miR-194-5p with siMALAT-1,PD-L1 expression was elevated.Co-transfection of miR-194-5p with siXIST did not have an impact on PD-L1 expression.Upon co-transfection of miR-194 with siTSIX,PD-L1 expression was upregulated.Interestingly,the same PD-L1 expression pattern was observed following miR-155-5p cotransfections.Oleuropein treatment of Huh-7 cells reduced the expression profile of PD-L1,XIST,and miR-155-5p,upregulated the expression of miR-194-5p and had no significant impact on the MALAT-1 expression profile.CONCLUSION This study reported a novel finding revealing that opposing acting miRNAs in HCC,have the same impact on PD-1/PD-L1 immune checkpoint by sharing a common signaling pathway.

Global optimality conditions for quadratic 0-1 programming with inequality constraints

Quadratic 0-1 problems with linear inequality constraints are briefly considered in this paper.Global optimality conditions for these problems,including a necessary condition and some sufficient conditions,are presented.The necessary condition is expressed without dual variables.The relations between the global optimal solutions of nonconvex quadratic 0-1 problems and the associated relaxed convex problems are also studied.

A Tabu Search Algorithm for Quadratic 0-1 Programming Problem

In this paper,quadratic 0-1 programming problem (I) is considered, in terms of its features quadratic 0-1 programming problem is solved by linear approxity heurstic algrothm and a developed tabu search ahgrothm .

Verifying the Effects of an Education Program Leveraging Information Technology to Promote Cervical Cancer Screening in Women Aged 20 - 29—A One-Year Longitudinal Study

The purpose of this research is to implement an IT-based education program in order to promote cervical cancer screenings for women aged 20 - 29 years, as well as to examine the results of said program. This is a longitudinal/comparative study of two groups, one for which the program was implemented (the intervention group), and the other for which it was not (the control group). The program consisted of attending a health lecture and encouragement to be screened one month, six months, and one year later sent through IT-based methods. The target was unmarried women aged 20 - 29 who had neither previously given birth nor had been screened for cervical cancer in a period one year prior. They were divided into two groups, the intervention group (n = 142) and control group (n = 145). The effectiveness of the program was assessed via an initial survey and further surveys six months and one year later. Results were based on the Japanese version of the Health Belief Model Scale for Cervical Cancer and the Pap Smear Test (HBMSCCPST), knowledge scores in the categories of Healthy Lifestyles, Cervical Cancer, Cervical Cancer Screening, and screening behavior. A two-way ANOVA of the HBMSCCPST subscales and knowledge scores in the initial, six-month, and one-year surveys was performed, showing interaction in Cervical Cancer (p = 0.00). Main effects were observed in Cervical Cancer Screening (p = 0.00) and Healthy Lifestyles (p = 0.00). Regarding the amount of change from the initial survey, knowledge scores in the Cervical Cancer (p = 0.027) and Cervical Cancer Screening (p = 0.016) categories were significantly higher in the intervention group than in the control group. There was no significant difference in cervical cancer screening rates (p = 0.26) between the two groups. However, a small-degree effect size was observed for Benefits, Seriousness, and Susceptibility subscales in both examinees and non-examinees. Although the educational program of this study was effective in improving the knowledge of women in their twenties, there was little improvement in HBMSCCPST and it did not lead to the promotion of cervical cancer screening. In order to raise interest in cervical cancer screening, it is necessary to consider useful content to guide women to consult with healthcare professionals, a long-term population approach, and organizational structure of consultation.

GRAPHICAL COMPUTING METHOD ON FOUR-BAR LINKAGE(1)——Inflexion Circle,Focal Axis,Ball's Point,Centering-point Curve and Circling-point Curve For Four ISPs,Coupler Curves Based on Ball's Points——

By means of programs GTMPAC based- on generalized triangle method,analysis and synthesis of mechanism design in accordance with absolutely graphicalmethod( absolutely germetrical method) are developed.In this paper,we make aspecial study about centering- point curve and circling- point curve and couplercurves based on Ball’s points.

Nondifferentiable Multiobjective Programming under Generalized d_I-G-Type I Invexity

To relax convexity assumptions imposed on the functions in theorems on sufficient conditions and duality,new concepts of generalized dI-G-type Ⅰ invexity were introduced for nondifferentiable multiobjective programming problems.Based upon these generalized invexity,G-Fritz-John （G-F-J） and G-Karnsh-Kuhn-Tucker （G-K-K-T） types sufficient optimality conditions were established for a feasible solution to be an efficient solution.Moreover,weak and strict duality results were derived for a G-Mond-Weir type dual under various types of generalized dI-G-type Ⅰ invexity assumptions.

The Develoyment Program of Energy Conservation and Comprehensive Utilization 2005-2007

Jointly published by 14 Department headed by Standardization Administraion ofChina(SAC) Subtitle: China will formulate and reformulate 926 standards for energy conservation andcomprehensive u-tilization during 2005 and 2007. In the past few days, 14 departments jointlypublished the Development Program of Energy Conservation and Comprehensive Utilization 2005-2007,(Program for short). They are Standardization Administration of China, National Development andReform Commission, Ministry of Land and Resources, Ministry of Establishment, Ministry ofCommunications, Ministry of Information Industry, Ministry of Waster Resources, Ministry ofAgriculture, Ministry of Commerce, General Administration of Quality Supervision, Inspection andQuarantine of the PRC, State Environmental Protection Administration, State Forestry Bureau, StateOceanic Administration and China Meteorological Bureau.

基于ESP8266的单片机无线烧写器的设计与实现

为了简化51单片机烧写流程,克服传统单片机有线烧写的弊端,设计了基于ESP8266的单片机无线烧写器。该无线烧写器以ESP8266作为主控单元,通过Wi-Fi网络使用TCP协议与PC上的TCP服务器进行通信,获取烧写BIN文件,然后通过ESP8266内的烧写控制程序,适时对51单片机的电源进行控制,实现51单片机程序的自动烧写。测试结果验证了该设计方案的正确性和可用性。

应用VB实现AGA8-92DC气体压缩因子计算方法

天然气压缩因子在天然气工程计算中具有重要的地位,其计算方法有多种,其中就包括由美国燃气协会提出的基于气体组成来计算压缩因子的AGA8-92DC方程。根据GB/T 17747.2-2011标准所给出的AGA8-92DC方法计算过程,用Visual Basic6.0 program编写了计算程序。为了验证程序的正确性,分别将程序计算值与GB/T 17747.2标准提供的计算值和含碳贫气气样实测值进行了对比,且获得了较小的计算误差。由计算值与实测值的对比结果可知,通过Visual Basic6.0 program所编写的AGA8-92DC方法计算程序而得到的计算结果能够满足工程上的要求;对于中高压含碳贫气,AGA8-92DC方法具有较高的计算准确度;当气体温度升高时,AGA8-92DC方法的计算误差将有所减小。

一种支持ISP的Flash在嵌入式设计中的应用

在基于VxWorks的嵌入式系统开发过程中,设计和调试板卡支持包BSP(Board Support Package)是一个较复杂的问题。在对比常用的bootrom实现的方法后,提出一种支持在系统编程(ISP)的Flash在嵌入式中的应用实现方法,并对该方法的硬件电路设计和器件编程进行较详细说明。该设计方法的引入降低了BSP开发难度,提高开发效率。

ISP技术在《数字电路》课程设计中的应用

ISP技术是国内外最先进的设计最新一代数字系统的有力工具与器件之一,ISP器件也正被越来越多的电子技术设计人员所应用。介绍了ISP技术的功能和特点,并运用ISP技术灵活方便的特点在数字电路课程设计中设计了电子钟这个具体实例。通过这个实例的分析,丰富了实验教学手段,培养了学生实验操作能力。

利用并口实现AT89S系列单片机ISP功能的一种方法

ATMEL 公司新近推出的 AT89S系列单片机提供一个同步串行通信接口 SPI(Serial Peripheral Interface),用户能够通过计算机并行接口或串行接口对其内部程序存储器编程。本文介绍了 AT89S系列单片机 ISP(In System Progamming)功能的原理,实现AT89S 系列单片机 ISP 功能的一种方法(包括编程电缆和应用软件),最后还对单片机 ISP 功能的应用作了进一步的说明。

利用串口实现AVR单片机ISP功能的一种方法

ATMEL公司研发的AVR单片机提供一个同步串行通信接口SPI(Serial Peripheral Interface),用户能够通过计算机的并行接口或串行接口对其内部程序存储器编程。实际上,利用串口实现AVR单片机ISP(In Sys-tem Programmable)功能就是使用计算机的串口来驱动AVR单片机ISP串行编程所需的信号波形,从而实现计算机对AVR单片机的编程。首先介绍AVR单片机ISP功能的原理,然后讨论利用串口实现AVR单片机ISP功能的一种方法,给出编程电缆原理图、编程电缆监控程序通信协议和编程工具软件。

基于JTAG和ISP的新型可编程电路

针对工程中通过JTAG和ISP编程出现的无法下载和电源芯片烧毁故障,分析基于锂电池的嵌入式软开关工作原理、编程过程,总结了导致故障的原因,提出了JTAG和ISP编程故障的解决方案,设计新型可编程电路,并通过一个工程实例进行了验证。