Other Cities’Distribution Institutions for Publishing Books in Modern Chinese Libraries:Taking Huating Bookstore,the Shanghai General Distribution Office of Modern Zhejiang Library,as an Example

Many modern Chinese libraries have established distribution agencies in other cities to distribute books published or sold on consignment.Huating Bookstore,which was once the Shanghai General Distribution Office of Zhejiang Library in modern times,is one of them.On the basis of a brief description of modern Zhejiang Library and its book publishing,as well as the overview of Huating Bookstore,the paper introduces and analyzes the ZPL Publishing Book Catalogue sent by Huating Bookstore,and the ZPL publishing and selling consignment books issued by Huating Bookstore.It points out that Huating Bookstore is a bridge between the ZPL located in Hangzhou and various retail ZPL publishing bookstores in Shanghai.Their production and sales relationship is a mutually beneficial one.

An efficient method for constructing a random insertional mutant library for forward genetics in Nannochloropsis oceanica

Insertional mutation,phenotypic evaluation,and mutated gene cloning are widely used to clone genes from scratch.Exogenous genes can be integrated into the genome during non-homologous end joining(NHEJ)of the double-strand breaks of DNA,causing insertional mutation.The random insertional mutant library constructed using this method has become a method of forward genetics for gene cloning.However,the establishment of a random insertional mutant library requires a high transformation efficiency of exogenous genes.Many microalgal species show a low transformation efficiency,making constructing random insertional mutant libraries difficult.In this study,we established a highly efficient transformation method for constructing a random insertional mutant library of Nannochloropsis oceanica,and tentatively tried to isolate its genes to prove the feasibility of the method.A gene that may control the growth rate and cell size was identified.This method will facilitate the genetic studies of N.oceanica,which should also be a reference for other microalgal species.

Effects of Furniture Ergonomics on Student’s Satisfaction in a Library Facility

Building occupants are not immune to ill-health as a result of time they spend in a building.This paper seeks to examine the effects furniture ergonomics have on student’s satisfaction in the library of Universiti Teknologi Malaysia.A pilot survey was initially conducted in the library through a one-to-one interaction with students to fetch their opinions on the general effects of the furniture.An observation through several walkthroughs was also conducted by the researchers to compare and validate responses obtained.Two hundred and sixty-five students that come from fifteen nationalities are surveyed.A structured questionnaire is used to collect data on the respondents’opinions on the size,shape,arrangement and comfort of the furniture.Eta cross tabulation,Spearman’s rho and Kendall’s Tau-b are used to establish relationships.Results show that amongst the effects studied,there are significant positive relationships between students’satisfaction of furniture ergonomics as against back-strain and lack of concentration.This implies that the more the furniture arrangements,size and shape are perceived unsatisfactory,the more their effects on back-strain and lack of concentration towards the students.This paper further recommends that library management should see to designing IEQ(Indoor Environmental Quality)guidelines that will mitigate the effects of furniture ergonomics thus improving student’s satisfaction.

Design and Implementation of Library Seating Management System

The library seating management system is an important part of the library’s modern services. The system is based on the Spring and Spring Boot framework, and the system realizes a series of functions such as real-time query, reservation, and cancellation of seat resources, providing users with great convenience. With a simple operation, users can know the availability of seats in the library in real time and reserve them according to their needs. At the same time, the system also provides rich management functions, enabling administrators to easily configure and manage seat resources. The addition, deletion, modification and review of users, the generation of seats, the viewing of user usage records, and the addition or deletion of points for users’ usage can also be carried out. This not only improves the management efficiency, but also provides more scientific and accurate data support for the management of the library. The system not only optimizes the user experience, but also promotes the scientific management and efficient utilization of library resources, and provides strong support for the modern services of the library.

Research on the Construction of a Comprehensive Teaching Case Library for Ergonomics under the OBE Concept

In recent years,case-based teaching has become a hot spot in higher education reform,and the construction of case libraries has received increasing attention.The development of teaching case libraries not only promotes changes in talent training models but also enhances teaching effectiveness.Taking the ergonomics course as an example,this paper explores the construction of a comprehensive teaching case library for ergonomics under the outcome-based education concept,providing a reference for future ergonomics teaching case library development.

Integrated Project Library Exploration in Landscape Design Teaching for Environmental Design Majors

To enhance our talent cultivation model through“school-enterprise cooperation and industry-teaching fusion”,we aim to improve the“4321”Industry-Education Integration System.This includes actively promoting the use of case banks and project banks in teaching to develop students’practical engineering skills through hands-on application of professional knowledge.Additionally,landscape design courses emphasize practical learning experiences to implement the fundamental goal of“cultivating morality”.Guided by enhancing students’practical skills,we ensure alignment with course objectives and professional training requirements,emphasizing the seamless integration of theory and practice.

Analysis of the Books Issued by Yadong Library, Published by Peking University Publishing Department: Taking Several Bibliography Advertisements Carried in Newspapers and Magazines From 1919-1922 as Examples

Based on several bibliographic advertisements carried in newspapers and magazines from 1919 to 1922 for books published by Peking University Publishing Department,and issued by Yadong Library,the paper analyzes the characteristics of the books published by Peking University Publishing Department during this period,and points out that Yadong Library entered its golden period of development due to move from the alley to the main road and obtain the management rights of the books published by Peking University Publishing Department.

Bacterial artificial chromosome library construction of root-knot nematode resistant pepper genotype HDA149 and identification of clones linked to Me3 resistant locus

Pepper （Capsicum annuum. L.） is a widely cultivated vegetable crop worldwide and has the second largest planting area and the first largest vegetable output and value in China. Pepper root-knot nematode （Meloidogyne spp.） is one of the most serious pests of pepper, which caused huge losses every year. Previous studies showed that the Me3 gene is resistant to a wide range of Meloidogyne species, including M. arenaria, M. javanica, and M. incognita. HDA149, a double haploid pepper genotype, harboring the root-knot nematode resistance gene Me3, was used to construct bacterial artificial chro- mosome library （BAC） via the vector of CopyControFM pCC1 in this study. The library consists of 210 200 BAC clones and is equivalent to 5.3 pepper genomes. The average insert size is 95 kb, and most of them are 90-120 kb; but the empty clones are less than 3%. In order to screen the BAC library easily, 550 super pools with 384 BAC clones of each pool were further developed in this study. Specific primers from Me3 gene locus were used for BAC library screening, and more than 20 positive BAC clones were obtained. Then the selected positive BAC clones were analyzed by restriction enzyme digestion, BAC-end sequencing, marker development, and new positive BAC clones exploration, respectively. Finally, the contig with total length of about 300 kb linked to the Me3 locus was constructed based on chromosome walking strategy, which made a solid foundation for the cloning of the important root-knot nematode resistance gene Me3.

Long-Term Exclusion of Grazing Increases Soil Microbial Biomass but Not Diversity in a Temperate Grassland

Restoration of grassland such as exclusion of grazing has been considered to increase aboveground plant diversity and soil fertility. However, knowledge on the effect of long-term exclusion of grazing on soil bacterial community structure and diversity is not well understood. The two sites were selected in the Inner Mongolian grassland, i.e., one fenced off since 1979 (UG79) and the other continually grazed by sheep (FG) all along. Soil microbial biomass was measured using fumigation method and bacterial community structure and diversity were assessed using methods of Denaturing Gradient Gel Electrophoresis (DGGE) and clone library. Results showed that the UG79 soil had significantly higher microbial biomass carbon and nitrogen compared with the FG soil. There was a clear separation in soil bacterial community structure, but not in bacterial diversity between the two sites. Moreover, 55 clones from the UG79 soil and 56 clones from the FG soil were selected and sequenced. Phylogenetic analysis of all clone sequences indicated that bacterial communities were dominated by the groups of Actinomycetes, Proteobacteria and Bacteroidetes, but there were no significant differences in bacterial diversity between the two sites, consistent with the results obtained from DGGE. The results highlighted that although long-term exclusion of grazing increased soil microbial biomass, but it did not harbor higher bacterial diversity compared with freely grazed site.

Advances in Normalized cDNA Library and Its Applications

The cDNA library normalized by reassociation is a newly-developed and effective platform for EST acquisition and gene discovery.It decreases the prevalence of clones representing abundant transcripts and dramatically increases the efficiency of random sequencing and rare gene discovery.The principle,procedure and applications of normalized cDNA library were reviewed in this paper,which provides theoretical basis for the development of normalized cDNA library and discover more novel genes.

Construction of a cDNA Library from the Testis and Sequence Analysis of the Ubiquitin Gene from Rana nigromaculata

A full-length cDNA library from the testis of dark-spotted frogs （ Rana nigromaculata ） was constructed with the SMART （switching mechanism at 5＇ end of RNA transcript） technique. Total RNA was extracted from the testis and reverse transcripted into full-length cDNA using PowerScript reverse transcriptase. The first-strand cDNA was amplified using long-distance PCR （LD-PCR）. After Sfi Ⅰ digestion and fractionation, cDNA （ 〉 500 bp） was ligated to λ TriplEx2 vector and packaged with GigapackⅢ Gold Packaging Extract. The titers of optimal primary libraries were 2.0×10^6 pfu/mL and 2.4 × 10^6 pfu/mL and the tlters of the amplified libraries were 0.48 × 10^9 pfu/mL and 3.0 × 10^9 pfu/ mL, respectively. The percentages of recombinant clones of primary libraries and amplified libraries were all over 90%. The libraries were converted into pTriplEx2 plasmids in E. coli BM 25.8 strain. The insert sizes were measured by PCR which showed most fragments were over 500 bp and the average length was 1.0 kb approximately. A positive clone of 1 171 bp was sequenced and named RnUb based on sequence similarity with the known ubiquitin genes in Gen- Bank. This sequence was a full-length cDNA with complete coding sequences, which indicated that the library built a base for screening the full-length cDNA. These data showed that this library attained to the requirements of a standard cDNA library. This library provided a useful resource for the functional genomic research of Rana nigromaculata.

Construction of Mutation Library in Haemophilus parasuis by Inserting Tn5 Transposon and the Screening of Attenuated Strain

[Objective] The study aimed to obtain attenuated strain of Haemophilus parasuis.[Method] Tn5 transposon technology was used to construct a library of mutants.Positive mutants were screened by kanamycin resistance.False positive was identified by PCR and then removed.Mice were infected to detect the virulence of mutants.The bionomics of attenuated strains were detected,too.[Result] The attenuated mutants showed similar reproductive activity to that of wild strain.The virulence of mutants was still stable after 30 passages.[Conclusion] This study provided foundation for exploring the virulence factors and pathogenic mechanism of HPS.

图书馆与读者之间的桥梁——香港浸会大学图书馆的Library online tutorial

本文介绍浸会大学图书馆建设的 library online tutorial,对 library online tutorial 的建设意义,目的加以描述,主要介绍 library online tutorial 中五个模块的主要内容、功能,并从中总结 library online tutorial 中蕴含的理念。

Library-Inside:AI赋能图书馆新质生产力的一种基础模型

AI正引发知识服务的生产力×生产关系的革命性变革,迫切需要推动AI与图书馆服务深度融合,打造符合新的发展理念的先进生产力质态。本文提出,从第一性原则思维出发,面向复杂适应性系统,以问题驱动和场景驱动的创新和设计驱动的创新,不断演进发展AI赋能的图书馆服务的新质生产力。在回归原点分析“人们到图书馆干什么”的基础上,基于大语言模型+AI智能体提供的能力,提出Library-Inside作为AI赋能图书馆新质生产力的一种基础模型,并描述了三种可能的实现模式:Library-Inside 1.0作为用户知识应用的智能助手,Library-Inside 2.0作为用户知识生产的智慧座舱和Library-Inside 3.0(Library×)打造用户AI赋能知识生产力的生态养育站。本文指出,要以战略创新推动图书馆新质生产力发展,要注意克服战略创新中的内在的结构性缺失和系统性偏差。

Construction of Midgut Tissue-Specific cDNA Library of Bombyx mandarina M. and Isolation and Sequence Analysis of Serine Protease Gene Fragment

[Objective] The aim of the study is to construct cDNA library of midgut tissue of wild silkworm and isolate the serine protease gene. [Method] The midgut tissue-specific cDNA library of wild silkworm was constructed via cDNA Library Construction Kit (TaKaRa), then the serine protease gene was cloned via sequencing of the yielded cDNA library. [Result] The titer of cDNA library reached 6.2×105 pfu/ml, average insert size was about 1.2 kb. The serine protease gene cDNA fragment was obtained from colony sequencing (Accession No: EU672968). The nucleotide sequence of the cloned 854 bp fragment encodes 284 amino acid residues. Homology analyses showed some homology between putative amino acid sequence of the cloned fragment and amino acid sequences of serine proteases from other ten insects. [Conclusion] The results may avail to reveal the resistance of silkworm and wild silkworm to exotic intrusion.

中国图书馆外文文献科研产出探析——以SSCI平台INFORMATION SCIENCE & LIBRARY SCIENCE为例

图书馆作为高校科研发展的依托单位,其科研产出也逐渐受到关注。筛选我国SSCI收录的图情专业与图书馆的科研产出,利用CiteSpaceⅡ及Googleearth等软件对样本进行计量分析,研究来源期刊、合作情况及研究热点等,以期为图书馆科研能力的提升提供支持。

Construction and Quality Analysis of Full-length cDNA Library of Phyllostachys heterocycla Germinating Seeds

[Objective] This study aimed to construct the full-length cDNA library for ger- minating seeds of Phyllostachys heterocycla [Method] Germinating seeds of P. hetero- cycla were used as experimental materials to construct the full-length cDNA library by using Oligo-capping method. [Result] The constructed library has a total capacity of 6.5×10^6 recombinant clones, and a low proportion of clones without inserted frag- ments; the size of inserted fragments ranges between 0.3-5.0 kb, with strict classifi- cation and ideal consistency. Furthermore, the proportion of clones harboring long in- serted fragments （1.0-5.0 kb） is as high as 30%, achieving the standard for high- quality full-length cDNA library. [Conclusion] The full-length cDNA library of germinat- ing seeds of P. heterocycla was successfully constructed, which laid important foun- dation for the functional genomics research of bamboo plants.

A Cosmid Library Constructed to the Elite Rice Cultivar “Minghui 63”

A genomic DNA library was constructed to the elite rice cultivar “Minghui 63” using the cosmid SuperCos1 as the vector. The library consisted of 45000 clones with average insert size about 40 kb. It was estimated that this library had a capacity of 4.2 times equivalent of the haploid genome of rice.

Sports Education Library Information Services

Library website,is the first window for readers to understand library information services.Sports Education academy library fully take advantage of homepage,combine open access resource searched with the library collections,after targeted collection,selection,sorting,processing,clustering or reorganization,establish a navigation system of open access resource of physical Sports Education.

Computer vision-aided DEM study on the compaction characteristics of graded subgrade filler considering realistic coarse particle shapes

The compaction quality of subgrade filler strongly affects subgrade settlement.The main objective of this research is to analyze the macro-and micro-mechanical compaction characteristics of subgrade filler based on the real shape of coarse particles.First,an improved Viola-Jones algorithm is employed to establish a digitalized 2D particle database for coarse particle shape evaluation and discrete modeling purposes of subgrade filler.Shape indexes of 2D subgrade filler are then computed and statistically analyzed.Finally,numerical simulations are performed to quantitatively investigate the effects of the aspect ratio(AR)and interparticle friction coefficient(μ)on the macro-and micro-mechanical compaction characteristics of subgrade filler based on the discrete element method(DEM).The results show that with the increasing AR,the coarse particles are narrower,leading to the increasing movement of fine particles during compaction,which indicates that it is difficult for slender coarse particles to inhibit the migration of fine particles.Moreover,the average displacement of particles is strongly influenced by the AR,indicating that their occlusion under power relies on particle shapes.The dis-placement and velocity of fine particles are much greater than those of the coarse particles,which shows that compaction is primarily a migration of fine particles.Under the cyclic load,the interparticle friction coefficientμhas little effect on the internal structure of the sample;under the quasi-static loads,however,the increase inμwill lead to a significant increase in the porosity of the sample.This study could not only provide a novel approach to investigate the compaction mechanism but also establish a new theoretical basis for the evaluation of intelligent subgrade compaction.