Influences of PGR, sucrose, and ammonium nitrate on in vitro flowering and fruiting from cotyledon explants of P. frutescens were studied. The regenerated shoots at 2-4. cm from cotyledon explants on MS medium supplem...Influences of PGR, sucrose, and ammonium nitrate on in vitro flowering and fruiting from cotyledon explants of P. frutescens were studied. The regenerated shoots at 2-4. cm from cotyledon explants on MS medium supplemented with 0.5 mg L^-1 BA and 1.0 mg L^-1IAA were excised and transferred to MS medium containing 30 g L^-1 sucrose, 8.25 g L^-1 ammonium nitrate, and 1.0 mg L^-1 BA. Following 40 d of culture, 86.2% of them flowered and set seeds. These seeds were germinable and developed into flowering plants in the fields. This study provides a simple system for rapid breeding of P. frutescens and studying the physiological mechanism of flowering of plants.展开更多
Silver nitrate induced MS media enhanced multiple shoot regeneration and in vitro flowering from axillary bud and leaf explants of Solanum nigrum (L.) an important antiulcer medicinal plant. Healthy axillary bud and l...Silver nitrate induced MS media enhanced multiple shoot regeneration and in vitro flowering from axillary bud and leaf explants of Solanum nigrum (L.) an important antiulcer medicinal plant. Healthy axillary bud and leaf explants were inoculated on MS medium supplemented with BAP/Kn (1.0 - 2.0 mg/l) in combination with IAA/NAA (0.5 mg/l) and AgNO<sub>3</sub> (0.1 - 1.0 mg/l). The explants were responded effectively and good regeneration frequencies were observed in all the combinations of silver nitrate tested when compared to control. Maximum number of multiple shoots (34.3) was found in leaf explants cultured on MS media supplemented with BAP (2.0 mg/l), NAA (0.5 mg/l) and AgNO<sub>3</sub> (0.4 mg/l). These regenerated shoots were sub-cultured on to the flowering media. Maximum number of in vitro flowers (12) was obtained from axillary bud explants in BAP (2.0 mg/l), Kn (1.0 mg/l), IAA (0.5 mg/l) and AgNO<sub>3</sub> (6.0 mg/l) supplemented media when compared to leaf (4) and control. All the in vitro raised shoots were transferred to rooting medium supplemented with NAA, IBA (1.0 - 2.0 mg/l) and AgNO<sub>3</sub> (0.1 - 0.6 mg/l). The best rooting response (24.6) was observed in 2.0 mg/l IBA + 0.4 mg/l AgNO<sub>3</sub>. The well rooted plantlets were transferred to polybags containing soil + vermiculate in 1:1 ratio for hardening and finally the hardened plantlets were transferred to field conditions.展开更多
基金supported by the Science Foundation Project of Chongqing Normal University and Gansu Province,China(QS041-C31-09).
文摘Influences of PGR, sucrose, and ammonium nitrate on in vitro flowering and fruiting from cotyledon explants of P. frutescens were studied. The regenerated shoots at 2-4. cm from cotyledon explants on MS medium supplemented with 0.5 mg L^-1 BA and 1.0 mg L^-1IAA were excised and transferred to MS medium containing 30 g L^-1 sucrose, 8.25 g L^-1 ammonium nitrate, and 1.0 mg L^-1 BA. Following 40 d of culture, 86.2% of them flowered and set seeds. These seeds were germinable and developed into flowering plants in the fields. This study provides a simple system for rapid breeding of P. frutescens and studying the physiological mechanism of flowering of plants.
文摘Silver nitrate induced MS media enhanced multiple shoot regeneration and in vitro flowering from axillary bud and leaf explants of Solanum nigrum (L.) an important antiulcer medicinal plant. Healthy axillary bud and leaf explants were inoculated on MS medium supplemented with BAP/Kn (1.0 - 2.0 mg/l) in combination with IAA/NAA (0.5 mg/l) and AgNO<sub>3</sub> (0.1 - 1.0 mg/l). The explants were responded effectively and good regeneration frequencies were observed in all the combinations of silver nitrate tested when compared to control. Maximum number of multiple shoots (34.3) was found in leaf explants cultured on MS media supplemented with BAP (2.0 mg/l), NAA (0.5 mg/l) and AgNO<sub>3</sub> (0.4 mg/l). These regenerated shoots were sub-cultured on to the flowering media. Maximum number of in vitro flowers (12) was obtained from axillary bud explants in BAP (2.0 mg/l), Kn (1.0 mg/l), IAA (0.5 mg/l) and AgNO<sub>3</sub> (6.0 mg/l) supplemented media when compared to leaf (4) and control. All the in vitro raised shoots were transferred to rooting medium supplemented with NAA, IBA (1.0 - 2.0 mg/l) and AgNO<sub>3</sub> (0.1 - 0.6 mg/l). The best rooting response (24.6) was observed in 2.0 mg/l IBA + 0.4 mg/l AgNO<sub>3</sub>. The well rooted plantlets were transferred to polybags containing soil + vermiculate in 1:1 ratio for hardening and finally the hardened plantlets were transferred to field conditions.
