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In vitro Screening of Fusarium Wilt-resistant Germplasm Resources of Red Edible Seed Watermelon( Citrullus lanatus ssp. vulgaris var. megalaspermus Lin et Chao) 被引量:2
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作者 Tangjing LIU Ke ZHANG +2 位作者 Yuming LU Rixin HONG Suping WU 《Agricultural Biotechnology》 CAS 2014年第1期50-53,共4页
[ Objective] This study aimed to establish an appropriate technology system for in vitro screening of Fuzarium wilt-resistant germplasm resources of red edible seed watermelon and obtain variants resistant to fusaric ... [ Objective] This study aimed to establish an appropriate technology system for in vitro screening of Fuzarium wilt-resistant germplasm resources of red edible seed watermelon and obtain variants resistant to fusaric acid, thus providing resistant materials for breeding Fusarium wilt-resistant red edible seed watermel- on. [ Method] Using Zhongxin No. 1 red edible seed watermelon advemitious buds as screening materials and fusaric acid (FA) as a stress agent, in vitro screen- ing of Fusarium wilt-resistant red edible seed watermelon clonal variants and identification of Fusarium wilt-resistance of the germplasm resources of red edible seed watermelon were performed. [ Result] The results showed that the appropriate FA for in vitro screening of Fusarium wilt-resistant red edible seed watermelon vari- ants was 15 mg/L. In vitro screening system for Fusarium wilt-resistant red edible seed watermelon variants was established preliminarily and FA-resistant regenera- ted plants were obtained. Among the 36 germplasm resources of red edible seed watermelon, there were 2 highly resistant materials, 6 moderately resistant materi- als, 11 slightly resistant materials and 17 highly susceptible materials. [ Conclusion] This study confirmed preliminarily that in vitro screening method is effective for obtaining resistant materials of red edible seed watermelon. 展开更多
关键词 Citrullus lanatas ssp. vulgaris var. megalaspermus Lin et Chao Germplasm resources Fusarium wilt Somaclonal variation Resistance in vitro screening in vitro identification
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