Dietary intake of 0.1% fucoxanthin significantly reduced lipid hydroperoxide levels of liver and abdominal white adipose tissue (WAT) of obese/diabetes KK-Ay mice. The fucoxanthin supplementation also significantly re...Dietary intake of 0.1% fucoxanthin significantly reduced lipid hydroperoxide levels of liver and abdominal white adipose tissue (WAT) of obese/diabetes KK-Ay mice. The fucoxanthin supplementation also significantly reduced blood glucose level and hepatic lipid contents of the mice. Oxidative stress is known to be induced in hyperglycemia and high fat conditions. Therefore, in vivo antioxidant activity of fucoxanthin found in the present study could be attributed to its anti-diabetic effect and its decreasing effect on hepatic lipids. On the other hand, little effect of fucoxanthin on lipid hydroperoxide levels was found in normal ICR mice. Although the content of fucoxanthin metabolites in the abdominal WAT of KK-Ay mice was about 50 times higher that in the liver, there was little difference in its In Vivo antioxidant activity between in the liver and in the abdominal WAT. These results suggest that well-known ability of fucoxanthin to scavenge active oxygen species and/or free radicals would not be a main reason to explain its In Vivo antioxidant activity.展开更多
Objective:To determine the free radical scavenging potentials pytochemical constituents of ethanol leaves extracts of Allamanda cathartica(A.cathartica)and Bixa orellann(B.orellana)and thus their effects in antimalari...Objective:To determine the free radical scavenging potentials pytochemical constituents of ethanol leaves extracts of Allamanda cathartica(A.cathartica)and Bixa orellann(B.orellana)and thus their effects in antimalarial activities.Methods:Both ethanol extracted plant samples were administered at 50 mg/mL,100 mg/mL and 200 mg/mL to Albino rats and then administered with CCl_4 at 1 mL/kg body weight,in liquid paraffin(1:1,v/v)for 2 days(negative control)and compared with 5%Tween 80(placebo)and vitamin E(positive control)pretreatments.Thiobarbituric acid reactive substances(TBARS),glutathione(GSH)and calalase(CAT)activities in blood and liver tissues were assessed.Results:In CCl,treated rats,TBARS levels significantly increased,while decreased GSH and CAT levels were recorded for both plant extracts.Generally,higher TBARS and GSH values were recorded for blood than for liver homogenates;with reverse trend observed for CAT level.Increased concentrations of A.cathartica extract recorded significant antioxidant levels similar to tocopherol(vitamin E).Reducing sugars,saponins,flavonoids were recorded for both species;alkaloids in A.cathartica and terpenoids in B.orellana.Conclusions:A.cathartica,possess phytochemicals that recorded significant antioxidative defense activities for blood and liver tissues with increasing concentration.However B.orellana did not record similar results.展开更多
The bacterial pathogen Helicobacter pylori(H. pylori) colonizes in over half of the world's population. H. pylori that establishes life-long infection in the stomach is definitely associated with gastro-duodenal d...The bacterial pathogen Helicobacter pylori(H. pylori) colonizes in over half of the world's population. H. pylori that establishes life-long infection in the stomach is definitely associated with gastro-duodenal diseases and a wide variety of non-gastrointestinal tract conditions such as immune thrombocytopenia. Triple therapy which consists of a proton pump inhibitor and combinations of two antibiotics(amoxicillin, clarithromycin or amoxicillin, metronidazol) is commonly used for H. pylori eradication. Recently, the occurrence of drug-resistant H. pylori and the adverse effect of antibiotics have severely weakened eradication therapy. Generally antibiotics induce the disturbance of human gastrointestinal microflora. Furthermore, there are inappropriate cases of triple therapy such as allergy to antibiotics, severe complications(liver and/or kidney dysfunction), the aged and people who reject the triple therapy. These prompt us to seek alterative agents instead of antibiotics and to develop more effective and safe therapy with these agents. The combination of these agents actually may result in lower a dose of antibiotics. There are many reports world-wide that non-antibiotic substances from natural products potentially have an anti-H. pylori agent. We briefly review the constituents derived from nature that fight against H. pylori in the literature with our studies.展开更多
The purpose of this study is to quantify the polyphenolic content and evaluated anti-inflammatory and antioxidant activity of four plants extracts of Annona senegalensis, Ipomoea batatas, Terminalia superba and Psidiu...The purpose of this study is to quantify the polyphenolic content and evaluated anti-inflammatory and antioxidant activity of four plants extracts of Annona senegalensis, Ipomoea batatas, Terminalia superba and Psidium guajava. Phenolic and flavonoid contents were determined by Folin-Ciocalteu and aluminum trichloride methods, respectively with ethanolic extract. The antioxidant activity was accessed by DPPH and FRAP methods. The results of the analysis of this study indicate that the ethanol extracts of the test plants (A. senegalensis, I. batatas, P. guajava and T. superba) are potential source of natural antioxidant and anti-inflammatory.展开更多
文摘Dietary intake of 0.1% fucoxanthin significantly reduced lipid hydroperoxide levels of liver and abdominal white adipose tissue (WAT) of obese/diabetes KK-Ay mice. The fucoxanthin supplementation also significantly reduced blood glucose level and hepatic lipid contents of the mice. Oxidative stress is known to be induced in hyperglycemia and high fat conditions. Therefore, in vivo antioxidant activity of fucoxanthin found in the present study could be attributed to its anti-diabetic effect and its decreasing effect on hepatic lipids. On the other hand, little effect of fucoxanthin on lipid hydroperoxide levels was found in normal ICR mice. Although the content of fucoxanthin metabolites in the abdominal WAT of KK-Ay mice was about 50 times higher that in the liver, there was little difference in its In Vivo antioxidant activity between in the liver and in the abdominal WAT. These results suggest that well-known ability of fucoxanthin to scavenge active oxygen species and/or free radicals would not be a main reason to explain its In Vivo antioxidant activity.
基金Supported by Centre for Research & Development (CUCERD),Covenant University(Grant No.CUCERD-0012-34/09)
文摘Objective:To determine the free radical scavenging potentials pytochemical constituents of ethanol leaves extracts of Allamanda cathartica(A.cathartica)and Bixa orellann(B.orellana)and thus their effects in antimalarial activities.Methods:Both ethanol extracted plant samples were administered at 50 mg/mL,100 mg/mL and 200 mg/mL to Albino rats and then administered with CCl_4 at 1 mL/kg body weight,in liquid paraffin(1:1,v/v)for 2 days(negative control)and compared with 5%Tween 80(placebo)and vitamin E(positive control)pretreatments.Thiobarbituric acid reactive substances(TBARS),glutathione(GSH)and calalase(CAT)activities in blood and liver tissues were assessed.Results:In CCl,treated rats,TBARS levels significantly increased,while decreased GSH and CAT levels were recorded for both plant extracts.Generally,higher TBARS and GSH values were recorded for blood than for liver homogenates;with reverse trend observed for CAT level.Increased concentrations of A.cathartica extract recorded significant antioxidant levels similar to tocopherol(vitamin E).Reducing sugars,saponins,flavonoids were recorded for both species;alkaloids in A.cathartica and terpenoids in B.orellana.Conclusions:A.cathartica,possess phytochemicals that recorded significant antioxidative defense activities for blood and liver tissues with increasing concentration.However B.orellana did not record similar results.
基金Supported by JSPS KAKENHI Grant,No.24590697 and No.24590698the Vietnam National Foundation for Science and Technology Development(NAFOSTED,106.99-2011.22)
文摘The bacterial pathogen Helicobacter pylori(H. pylori) colonizes in over half of the world's population. H. pylori that establishes life-long infection in the stomach is definitely associated with gastro-duodenal diseases and a wide variety of non-gastrointestinal tract conditions such as immune thrombocytopenia. Triple therapy which consists of a proton pump inhibitor and combinations of two antibiotics(amoxicillin, clarithromycin or amoxicillin, metronidazol) is commonly used for H. pylori eradication. Recently, the occurrence of drug-resistant H. pylori and the adverse effect of antibiotics have severely weakened eradication therapy. Generally antibiotics induce the disturbance of human gastrointestinal microflora. Furthermore, there are inappropriate cases of triple therapy such as allergy to antibiotics, severe complications(liver and/or kidney dysfunction), the aged and people who reject the triple therapy. These prompt us to seek alterative agents instead of antibiotics and to develop more effective and safe therapy with these agents. The combination of these agents actually may result in lower a dose of antibiotics. There are many reports world-wide that non-antibiotic substances from natural products potentially have an anti-H. pylori agent. We briefly review the constituents derived from nature that fight against H. pylori in the literature with our studies.
文摘The purpose of this study is to quantify the polyphenolic content and evaluated anti-inflammatory and antioxidant activity of four plants extracts of Annona senegalensis, Ipomoea batatas, Terminalia superba and Psidium guajava. Phenolic and flavonoid contents were determined by Folin-Ciocalteu and aluminum trichloride methods, respectively with ethanolic extract. The antioxidant activity was accessed by DPPH and FRAP methods. The results of the analysis of this study indicate that the ethanol extracts of the test plants (A. senegalensis, I. batatas, P. guajava and T. superba) are potential source of natural antioxidant and anti-inflammatory.
