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基于In-Cell Western对Ryanodine受体2检测方法的建立 被引量:1
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作者 王德军 屠珏 +3 位作者 褚燕青 陈方明 蔡月琴 朱科燕 《中国生物化学与分子生物学报》 CAS CSCD 北大核心 2016年第3期346-352,共7页
In-Cell Western技术是基于近红外激光成像系统而开发的检测技术,可应用于大分子ryanodine受体2(Ry R2)蛋白的检测。本研究通过对In-Cell Western固定剂的选择、细胞通透化、封闭液的选择、抗体工作浓度和心肌细胞密度等方面进行优化,... In-Cell Western技术是基于近红外激光成像系统而开发的检测技术,可应用于大分子ryanodine受体2(Ry R2)蛋白的检测。本研究通过对In-Cell Western固定剂的选择、细胞通透化、封闭液的选择、抗体工作浓度和心肌细胞密度等方面进行优化,建立心肌细胞Ry R2蛋白检测的InCell Western方法,并分别用SGF和Verapamil以及免疫细胞化学技术对研究结果及方法进行了验证。优化后的In-Cell Western参数:H9C2细胞的种板密度选择1×104个/孔,选择甲醛作为固定剂,细胞经过Triton X-100洗脱液通透化处理,选择酪蛋白作为封闭液,选择1∶500稀释的Ry R2一抗工作浓度。应用优化的参数检测心肌细胞Ry R2蛋白,并用前期已被证明能显著提高心肌细胞Ry R2蛋白荧光值的土茯苓黄酮和Verapamil进行验证,同时与免疫组化方法检测心肌细胞Ry R2蛋白相比,检测结果一致。表明本文建立的In-Cell Western方法检测大分子功能蛋白Ry R2是可行的。 展开更多
关键词 ryanodine受体2 in-cell western H9C2细胞
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In-cell Western和Cell ELISA在大分子量蛋白质相对定量中的应用 被引量:2
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作者 高翔 骞爱荣 +1 位作者 李京宝 商澎 《细胞生物学杂志》 CSCD 2009年第6期882-886,共5页
探讨两种新的检测方法—In-cell Western和Cell ELISA在大分子量蛋白质相对定量中的应用。将培养细胞原位固定、通透化、封闭,然后加入目标蛋白质的一抗、红外荧光标记的二抗或酶标记的二抗,使用双红外成像系统或多功能酶标仪对目标蛋... 探讨两种新的检测方法—In-cell Western和Cell ELISA在大分子量蛋白质相对定量中的应用。将培养细胞原位固定、通透化、封闭,然后加入目标蛋白质的一抗、红外荧光标记的二抗或酶标记的二抗,使用双红外成像系统或多功能酶标仪对目标蛋白质进行相对定量。通过这两种方法检测模拟失重环境对成骨细胞中大分子量骨架相关蛋白——微管微丝交联因子(microtubule actin cross-linking factor 1,MACF1)含量的影响,结果表明模拟失重环境促进了MACF1表达,且这两种方法检测结果一致。In-cell Western和Cell ELISA这两种检测方法灵敏迅速、操作简便,可以用于大分子量蛋白质的相对定量。 展开更多
关键词 in-cell western CELL ELISA 大分子量蛋白质 相对定量
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In-Cell Western检测细胞纤连蛋白
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作者 李京宝 胡丽芳 +2 位作者 张维 骞爱荣 商澎 《第四军医大学学报》 北大核心 2009年第19期1861-1863,共3页
目的:利用In-Cell Western技术建立检测细胞纤连白(cFN)的方法.方法:对微孔培养的成骨样细胞进行固定封闭后,使用单克隆抗体对FN进行标记,经红外荧光二抗育后使用Odysssey系统对细胞进行扫描成像和数据分析.在此基础之上优化了固定剂、... 目的:利用In-Cell Western技术建立检测细胞纤连白(cFN)的方法.方法:对微孔培养的成骨样细胞进行固定封闭后,使用单克隆抗体对FN进行标记,经红外荧光二抗育后使用Odysssey系统对细胞进行扫描成像和数据分析.在此基础之上优化了固定剂、通透化、封闭剂和一抗浓度.果:免疫印迹法证实了In-Cell Western方法检测的特异性,当MG-63细胞密度低于90%汇合度(细胞数约1.6×104/孔)时,信号与细胞密度呈现较好的线性关系,最低每孔4×103个MG-63细胞的cFN可被检出.结论:成功地建立了一种快速、经济且高通量的cFN检测方法并将其应用于细胞外基质研究. 展开更多
关键词 In—Cell western 纤连蛋白 细胞外基质 优化
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In-cell Western法测定人胰岛素生物学活性
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作者 王绿音 杨艳枫 +3 位作者 梁誉龄 张慧 李晶 梁成罡 《中国新药杂志》 CAS CSCD 北大核心 2023年第10期1000-1006,共7页
目的:利用in-cell Western(ICW)技术研究基于转基因细胞的人胰岛素生物学活性测定方法。方法:以CHO-INSR B1284为靶细胞,用不同浓度的人胰岛素刺激靶细胞,采用ICW技术检测人胰岛素受体酪氨酸磷酸化水平,进行人胰岛素的体外生物学活性测... 目的:利用in-cell Western(ICW)技术研究基于转基因细胞的人胰岛素生物学活性测定方法。方法:以CHO-INSR B1284为靶细胞,用不同浓度的人胰岛素刺激靶细胞,采用ICW技术检测人胰岛素受体酪氨酸磷酸化水平,进行人胰岛素的体外生物学活性测定;根据《中华人民共和国药典》2020年版通则9401“生物制品生物活性/效价测定方法”对该方法进行专属性、相对准确度、中间精密度、线性与范围等方法学验证;以重组人胰岛素国家标准品为参比品,采用本方法测定人胰岛素原料药及其他人胰岛素类似物的生物学活性相对效价。结果:不同浓度的人胰岛素作用于CHO-INSR B1284细胞后具有较好的剂量依赖性;5个效价浓度的待测品经8次测定,相对效价的几何均值分别为:(63.76±4.38)%,(78.01±5.97)%,(99.52±4.62)%,(122.84±7.4)%,(151.71±8.81)%,相对偏倚均在±5%范围内,对应的几何变异系数(GCV,%)均<11%,GCV的置信上限均<20%;采用该方法能有效检测人胰岛素及其胰岛素类似物的效价。结论:利用ICW技术的人胰岛素体外生物学活性测定法专属性强、准确度高、精密度好,可作为人胰岛素生物学活性的常规检测方法。 展开更多
关键词 生物学活性 人胰岛素 转基因细胞 in-cell western 方法学验证
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A Physics-informed Deep-learning Intensity Prediction Scheme for Tropical Cyclones over the Western North Pacific 被引量:1
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作者 Yitian ZHOU Ruifen ZHAN +4 位作者 Yuqing WANG Peiyan CHEN Zhemin TAN Zhipeng XIE Xiuwen NIE 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2024年第7期1391-1402,共12页
Accurate prediction of tropical cyclone(TC)intensity is challenging due to the complex physical processes involved.Here,we introduce a new TC intensity prediction scheme for the western North Pacific(WNP)based on a ti... Accurate prediction of tropical cyclone(TC)intensity is challenging due to the complex physical processes involved.Here,we introduce a new TC intensity prediction scheme for the western North Pacific(WNP)based on a time-dependent theory of TC intensification,termed the energetically based dynamical system(EBDS)model,together with the use of a long short-term memory(LSTM)neural network.In time-dependent theory,TC intensity change is controlled by both the internal dynamics of the TC system and various environmental factors,expressed as environmental dynamical efficiency.The LSTM neural network is used to predict the environmental dynamical efficiency in the EBDS model trained using besttrack TC data and global reanalysis data during 1982–2017.The transfer learning and ensemble methods are used to retrain the scheme using the environmental factors predicted by the Global Forecast System(GFS)of the National Centers for Environmental Prediction during 2017–21.The predicted environmental dynamical efficiency is finally iterated into the EBDS equations to predict TC intensity.The new scheme is evaluated for TC intensity prediction using both reanalysis data and the GFS prediction data.The intensity prediction by the new scheme shows better skill than the official prediction from the China Meteorological Administration(CMA)and those by other state-of-art statistical and dynamical forecast systems,except for the 72-h forecast.Particularly at the longer lead times of 96 h and 120 h,the new scheme has smaller forecast errors,with a more than 30%improvement over the official forecasts. 展开更多
关键词 tropical cyclones western North Pacific intensity prediction EBDS LSTM
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Quantitative effect of kerogen type on the hydrocarbon generation potential of Paleogene lacustrine source rocks,Liaohe Western Depression,China 被引量:1
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作者 Sha-Sha Hui Xiong-Qi Pang +7 位作者 Fu-Jie Jiang Chen-Xi Wang Shu-Xing Mei Tao Hu Hong Pang Min Li Xiao-Long Zhou Kan-Yuan Shi 《Petroleum Science》 SCIE EI CAS CSCD 2024年第1期14-30,共17页
Kerogen types exert a decisive effect on the onset and capacity of hydrocarbon generation of source rocks.Lacustrine source rocks in the Liaohe Western Depression are characterized by thick deposition,high total organ... Kerogen types exert a decisive effect on the onset and capacity of hydrocarbon generation of source rocks.Lacustrine source rocks in the Liaohe Western Depression are characterized by thick deposition,high total organic carbon(TOC)content,various kerogen types,and a wide range of thermal maturity.Consequently,their hydrocarbon generation potential and resource estimation can be misinterpreted.In this study,geochemical tests,numerical analysis,hydrocarbon generation kinetics,and basin modeling were integrated to investigate the differential effects of kerogen types on the hydrocarbon generation potential of lacustrine source rocks.Optimized hydrocarbon generation and expulsion(HGE)models of different kerogen types were established quantitatively upon abundant Rock-Eval/TOC/vitrinite reflectance(R_(o))datasets.Three sets of good-excellent source rocks deposited in the fourth(Es4),third(Es3),and first(Es1)members of Paleogene Shahejie Formation,are predominantly types I-II_(1),II_(1)-II_(2),and II-III,respectively.The activation energy of types I-II_(2)kerogen is concentrated(180-230 kcal/mol),whereas that of type III kerogen is widely distributed(150-280 kcal/mol).The original hydrocarbon generation potentials of types I,II_(1),II_(2),and III kerogens are 790,510,270,and 85 mg/g TOC,respectively.The Ro values of the hydrocarbon generation threshold for type I-III source rocks gradually increase from 0.42%to 0.74%,and Ro values of the hydrocarbon expulsion threshold increase from 0.49%to 0.87%.Types I and II_(1)source rocks are characterized by earlier hydrocarbon generation,more rapid hydrocarbon expulsion,and narrower hydrocarbon generation windows than types II_(2)and III source rocks.The kerogen types also affect the HGE history and resource potential.Three types(conventional,tight,and shale oil/gas)and three levels(realistic,expected,and prospective)of hydrocarbon resources of different members in the Liaohe Western Depression are evaluated.Findings suggest that the Es3 member has considerable conventional and unconventional hydrocarbon resources.This study can quantitatively characterize the hydrocarbon generation potential of source rocks with different kerogen types,and facilitate a quick and accurate assessment of hydrocarbon resources,providing strategies for future oil and gas exploration. 展开更多
关键词 Kerogen type Hydrocarbon generation potential Lacustrine source rocks Liaohe western depression
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Natural gas exploration potential and favorable targets of Permian Fengcheng Formation in the western Central Depression of Junggar Basin,NW China 被引量:1
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作者 TANG Yong HU Suyun +4 位作者 GONG Deyu YOU Xincai LI Hui LIU Hailei CHEN Xuan 《Petroleum Exploration and Development》 SCIE 2024年第3期563-575,共13页
Based on the organic geochemical data and the molecular and stable carbon isotopic compositions of natural gas of the Lower Permian Fengcheng Formation in the western Central Depression of Junggar Basin,combined with ... Based on the organic geochemical data and the molecular and stable carbon isotopic compositions of natural gas of the Lower Permian Fengcheng Formation in the western Central Depression of Junggar Basin,combined with sedimentary environment analysis and hydrocarbon-generating simulation,the gas-generating potential of the Fengcheng source rock is evaluated,the distribution of large-scale effective source kitchen is described,the genetic types of natural gas are clarified,and four types of favorable exploration targets are selected.The results show that:(1)The Fengcheng Formation is a set of oil-prone source rocks,and the retained liquid hydrocarbon is conducive to late cracking into gas,with characteristics of high gas-generating potential and late accumulation;(2)The maximum thickness of Fengcheng source rock reaches 900 m.The source rock has entered the main gas-generating stage in Penyijingxi and Shawan sags,and the area with gas-generating intensity greater than 20×10^(8) m^(3)/km^(2) is approximately 6500 km^(2).(3)Around the western Central Depression,highly mature oil-type gas with light carbon isotope composition was identified to be derived from the Fengcheng source rocks mainly,while the rest was coal-derived gas from the Carboniferous source rock;(4)Four types of favorable exploration targets with exploration potential were developed in the western Central Depression which are structural traps neighboring to the source,stratigraphic traps neighboring to the source,shale-gas type within the source,and structural traps within the source.Great attention should be paid to these targets. 展开更多
关键词 Junggar Basin western Central Depression Lower Permian Fengcheng Formation gas-generating potential gas-source correlation exploration target
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Geomorphic signatures and active tectonics in western Saurashtra,Gujarat,India
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作者 Bikramaditya Mondal Mery Biswas +1 位作者 Soumyajit Mukherjee Mohamedharoon A.Shaikh 《Geodesy and Geodynamics》 EI CSCD 2024年第1期82-99,共18页
Active tectonics in an area includes ongoing or recent geologic events.This paper investigates the tectonic influence on the subsidence,uplift and tilt of western Saurashtra through morphotectonic analysis of ten wate... Active tectonics in an area includes ongoing or recent geologic events.This paper investigates the tectonic influence on the subsidence,uplift and tilt of western Saurashtra through morphotectonic analysis of ten watersheds along with characteristics of relief and drainage orientation.Watersheds 7-9 to the north(N)are tectonically active,which can be linked with the North Kathiawar Fault System(NKFS)and followed by watersheds 6,10,1,4 and 5.Stream-length gradient index and sinuosity index indicate the effect of tectonic events along the master streams in watersheds 6-9.Higher R^(2)values of the linear curve fit for watershed 7 indicate its master stream is much more tectonically active than the others.The R^(2)curve fitting model and earthquake magnitude/depth analysis confirm the region to be active.The reactivation of the NKFS most likely led to the vertical movement of western Saurashtra. 展开更多
关键词 Active tectonics GEOMORPHOLOGY western Saurashtra ARCHEOLOGY North kathiawar fault system
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Geophysical and Crustal Movement Characteristic of Western Sichuan Basin
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作者 ZHOU Wenyue SUN Yanyun 《Acta Geologica Sinica(English Edition)》 SCIE CAS CSCD 2024年第S01期98-100,共3页
Analysis and research of large complex phenomena before and after the devastating earthquake to reduce the threat of natural disasters to human survival environment is of great significance.This paper analyzes the Wen... Analysis and research of large complex phenomena before and after the devastating earthquake to reduce the threat of natural disasters to human survival environment is of great significance.This paper analyzes the Wenchuan earthquake zone characteristics of gravity anomaly distributions,and then use edge detection and Euler deconvolution method to inverse Longmenshan gravity anomaly before earthquake.Fault distribution features and the general depth about the fault top of Longmenshan and its adjacent area before earthquake has been obtained.Morphology difference and possible earthquake formation have been analyzed through the Euler deconvolution result of gravity anomaly profile before and after the earthquake. 展开更多
关键词 edge detection gravity anomaly morphology difference western Sichuan Basin
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Food Hygiene and Safety Practices amongst Food Vendors in the Western Area, Sierra Leone
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作者 Philip John Kanu Hamid Turay 《Food and Nutrition Sciences》 CAS 2024年第6期421-431,共11页
Food safety and hygiene practices require a multisectoral approach including food, water, sanitation, waste management, transport, education, trade, policies and programs that enable safe food preparation, storage and... Food safety and hygiene practices require a multisectoral approach including food, water, sanitation, waste management, transport, education, trade, policies and programs that enable safe food preparation, storage and service. Unsafe food can cause illness keeping people from achieving their full potential and death. This was a descriptive study that uses a mixed method approach to derive insights into the characteristics of food vendors related to demography, knowledge, practices, infrastructure, compliance and recommendation for a policymaking framework. Using the Lemeshows’ sample size formula, 473 vendors from formal (restaurants) and informal (cookri-baffa/table top) sites were interviewed and observed. We found from discussions that respondents had a good understanding on how to keep food safe. However, observed practices were poor 93% handled food with their bare hands, 83% did not cover their hair, and 76% did not wear an apron whilst handling, preparing or serving food, 61% did not keep their finger nails clean or short and 57% did not wash their hand before preparing or serving food. Over half (51%) had access to a toilet but 32% reported their use required payment and emphasized their poor condition/inadequate management. Nearly half (47%) of the vending sites did not have a handwashing facility, with soap and water available. Only 7% reported having any authority oversight of food safety. Food safety and hygiene practices in most cookri shops and restaurants was ‘poor’ with very limited surveillance system in place by competent authorities for compliance of food operators. Hand washing, clean surroundings, and covered food were the most common and emphasized practices to mitigate the risks associated with unsafe food. 展开更多
关键词 Food Safety and Hygiene Formal and Informal Food Vendors western Area Sierra Leone
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Climate warming is significantly influenced by rising summer maximum temperatures: insights from tree-ring evidence of the Western Tianshan Mountains, China
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作者 Meng Ren Yu Liu +3 位作者 Qiufang Cai Qiang Li Huiming Song Changfeng Sun 《Journal of Forestry Research》 SCIE EI CAS CSCD 2024年第4期47-59,共13页
As one of the regions most affected by global cli-mate warming,the Tianshan mountains has experienced sev-eral ecological crises,including retreating glaciers and water deficits.Climate warming in these mountains is c... As one of the regions most affected by global cli-mate warming,the Tianshan mountains has experienced sev-eral ecological crises,including retreating glaciers and water deficits.Climate warming in these mountains is considered mainly to be caused by increases in minimum temperatures and winter temperatures,while the influence of maximum temperatures is unclear.In this study,a 300-year tree-ring chronology developed from the Western Tianshan Moun-tains was used to reconstruct the summer(June-August)maximum temperature(T_(max6-8))variations from 1718 to 2017.The reconstruction explained 53.1% of the variance in the observed T_(max6-8).Over the past 300 years,the T_(max6-8)reconstruction showed clear interannual and decadal vari-abilities.There was a significant warming trend(0.18°C/decade)after the 1950s,which was close to the increasing rates of the minimum and mean temperatures.The increase in maximum temperature was also present over the whole Tianshan mountains and its impact on climate warming has increased.The T_(max6-8) variations in the Western Tianshan mountains were influenced by frequent volcanic eruptions combined with the influence of solar activity and the sum-mer North Atlantic Oscillation.This study reveals that cli-mate warming is significantly influenced by the increase in maximum temperatures and clarifies possible driving mech-anisms of temperature variations in the Western Tianshan mountains which should aid climate predictions. 展开更多
关键词 Tree rings western Tianshan mountains Temperature change Climate warming
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Climate warming is significantly influenced by rising summer maximum temperatures:insights from tree-ring evidence of the Western Tianshan Mountains,China
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作者 Meng Ren Yu Liu +3 位作者 Qiufang Cai Qiang Li Huiming Song Changfeng Sun 《Journal of Forestry Research》 SCIE EI CAS CSCD 2024年第5期142-154,共13页
As one of the regions most affected by global climate warming,the Tianshan mountains has experienced several ecological crises,including retreating glaciers and water deficits.Climate warming in these mountains is con... As one of the regions most affected by global climate warming,the Tianshan mountains has experienced several ecological crises,including retreating glaciers and water deficits.Climate warming in these mountains is considered mainly to be caused by increases in minimum temperatures and winter temperatures,while the influence of maximum temperatures is unclear.In this study,a 300-year tree-ring chronology developed from the Western Tianshan Mountains was used to reconstruct the summer(June-August)maximum temperature(Tmax6-8) variations from 1718 to2017.The reconstruction explained 53.1% of the variance in the observed Tmax6-8.Over the past 300 years,the Tmax6-8reconstruction showed clear interannual and decadal variabilities.There was a significant warming trend(0.18 ℃/decade) after the 1950s,which was close to the increasing rates of the minimum and mean temperatures.The increase in maximum temperature was also present over the whole Tianshan mountains and its impact on climate warming has increased.The Tmax6-8variations in the Western Tianshan mountains were influenced by frequent volcanic eruptions combined with the influence of solar activity and the summer North Atlantic Oscillation.This study reveals that climate warming is significantly influenced by the increase in maximum temperatures and clarifies possible driving mechanisms of temperature variations in the Western Tianshan mountains which should aid climate predictions. 展开更多
关键词 Tree rings western Tianshan mountains Temperature change Climate warming
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Impacts of hydropower-induced flow alterations on composition and diversity of riparian vegetation in the Western Himalayas: A case study in Uttarakhand, India
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作者 Dharmveer KAINTURA Sabyasachi DASGUPTA Dhanpal Singh CHAUHAN 《Journal of Mountain Science》 SCIE CSCD 2024年第4期1272-1286,共15页
The increasing demand for water and energy resources has led to widespread dam construction,particularly in ecologically sensitive regions like the Himalayan Range.This study focuses on the Uttarakhand state in the We... The increasing demand for water and energy resources has led to widespread dam construction,particularly in ecologically sensitive regions like the Himalayan Range.This study focuses on the Uttarakhand state in the Western Himalayas,where hydroelectric projects(HEPs)have significantly altered river flow regimes.The research investigates the impact of flow alterations on the composition and structure of riparian vegetation in the Garhwal Himalayas,specifically analysing four rivers regulated by hydroelectric projects.Utilizing the paired-reach comparison method,control(undisturbed),diverted(downstream of barrage/dam),and altered flow conditions(downstream of water outlet)were examined.The research reveals diverse and unique riparian ecosystems,with 89 genera and 113 taxa identified,showcasing the dominance of families like Asteraceae and Lamiaceae.The study unveils the structural importance of key species such as Berberis asiatica and Artemisia nilagirica.The density,diversity,and richness of shrub and herb species vary significantly across flow conditions.Notably,altered flow conditions demonstrate resilience in vegetation structure,while diverted conditions exhibit decreased species richness and density.The study emphasizes the importance of nuanced environmental flow management for mitigating adverse effects on riparian biodiversity in the fragile Himalayan region.These findings contribute to the global discourse on dam impacts and riparian ecology,shedding light on the complexities of this dynamic relationship in a vulnerable ecosystem. 展开更多
关键词 Dam construction Water stress western Himalayas Disturbed flow regimes Riparian ecosystem
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Impact of typical environments in China’s western mountainous areas on the durability of railway concrete:a review
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作者 Jiahe Wang Yanbin Tan +3 位作者 Huajian Li Xinhua Zhong Jiaxuan Wang Yu Gao China 《Railway Sciences》 2024年第5期650-669,共20页
Purpose–This study aims to analyze the impact mechanism of typical environments in China’s western mountainous areas on the durability of railway concrete and propose measures to improve durability.Design/methodolog... Purpose–This study aims to analyze the impact mechanism of typical environments in China’s western mountainous areas on the durability of railway concrete and propose measures to improve durability.Design/methodology/approach–With the continuous promotion of infrastructure construction,the focus of China’s railway construction has gradually shifted to the western region.The four typical environments of large temperature differences,strong winds and dryness,high cold and low air pressure unique to the western mountainous areas of China have adverse effects on the durability of typical railway structure concrete(bridges,ballastless tracks and tunnels).This study identified the characteristics of four typical environments in the western mountainous areas of China through on-site research.The impact mechanism of the four typical environments on the durability of concrete in different structural parts of railways has been explored through theoretical analysis and experimental research;Finally,a strategy for improving the durability of railway concrete suitable for the western mountainous areas of China was proposed.Findings–The daily temperature difference in the western mountainous areas of China is more than twice that of the plain region,which will lead to significant temperature deformation and stress in the multi-layered structure of railway ballastless tracks.It will result in cracking.The wind speed in the western plateau region is about 2.5 to 3 times that of the plain region,and the average annual rainfall is only 1/5 of that in the plain region.The drying effect on the surface of casting concrete will significantly accelerate its cracking process,leading to serious durability problems.The environmental temperature in the western mountainous areas of China is generally low,and there are more freeze-thaw cycles,which will increase the risk of freeze-thaw damage to railway concrete.The environmental air pressure in the western plateau region is only 60%of that in the plain region.The moisture inside the concrete is more likely to diffuse into the surrounding environment under the pressure difference,resulting in greater water loss and shrinkage deformation of the concrete in the plateau region.The above four issues will collectively lead to the rapid deterioration of concrete durability in the western plateau region.The corresponding durability improvement suggestions from theoretical research,new technology development and standard system was proposed in this paper.Originality/value–The research can provide the mechanism of durability degradation of railway concrete in the western mountainous areas of China and corresponding improvement strategies. 展开更多
关键词 western mountainous areas CONCRETE DURABILITY ENVIRONMENT
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Track-Pattern-Based Characteristics of Extratropical Transitioning Tropical Cyclones in the Western North Pacific
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作者 Hong HUANG Dan WU +2 位作者 Yuan WANG Zhen WANG Yu LIU 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2024年第6期1251-1263,共13页
Based on the Regional Specialized Meteorological Center(RSMC)Tokyo-Typhoon Center best-track data and the NCEP-NCAR reanalysis dataset,extratropical transitioning(ET)tropical cyclones(ETCs)over the western North Pacif... Based on the Regional Specialized Meteorological Center(RSMC)Tokyo-Typhoon Center best-track data and the NCEP-NCAR reanalysis dataset,extratropical transitioning(ET)tropical cyclones(ETCs)over the western North Pacific(WNP)during 1951–2021 are classified into six clusters using the fuzzy c-means clustering method(FCM)according to their track patterns.The characteristics of the six hard-clustered ETCs with the highest membership coefficient are shown.Most tropical cyclones(TCs)that were assigned to clusters C2,C5,and C6 made landfall over eastern Asian countries,which severely threatened these regions.Among landfalling TCs,93.2%completed their ET after landfall,whereas 39.8%of ETCs completed their transition within one day.The frequency of ETCs over the WNP has decreased in the past four decades,wherein cluster C5 demonstrated a significant decrease on both interannual and interdecadal timescales with the expansion and intensification of the western Pacific subtropical high(WPSH).This large-scale circulation pattern is favorable for C2 and causes it to become the dominant track pattern,owning to it containing the largest number of intensifying ETCs among the six clusters,a number that has increased insignificantly over the past four decades.The surface roughness variation and three-dimensional background circulation led to C5 containing the maximum number of landfalling TCs and a minimum number of intensifying ETCs.Our results will facilitate a better understanding of the spatiotemporal distributions of ET events and associated environment background fields,which will benefit the effective monitoring of these events over the WNP. 展开更多
关键词 western North Pacific tropical cyclone extratropical transition fuzzy c-means clustering method
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Introduction of Western Medicines into China and Improvement on Decoctions during Modern China
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作者 ZHU Ruisi 《Chinese Medicine and Culture》 2024年第2期128-142,共15页
During the late Qing dynasty(1840 A.D.-1912 A.D.),a large quantity of Western medicines entered China,which continuously impacted the traditional Chinese medicine(TCM)market and revealed the shortcomings of Chinese me... During the late Qing dynasty(1840 A.D.-1912 A.D.),a large quantity of Western medicines entered China,which continuously impacted the traditional Chinese medicine(TCM)market and revealed the shortcomings of Chinese medicines.Some personages in the TCM community followed the trend of learning from the West,and attempted to reform TCM,with the improvement on decoction becoming an important aspect of this effort.Through debates and trials,the improvement on decoction underwent three stages of conceptual evolution:“taking Chinese medicines as the foundation and referring to the dosage forms of Western medicines”,“introducing Western techniques to serve the preparation of decoctions”and“integrating the theories of TCM and Western medicine to improve decoctions”.The study highlights the effective complementarity between modern TCM and Western medicine in the field of pharmacy,and provides valuable experience and support for the reevaluation of the value of TCM in contemporary society. 展开更多
关键词 western medicine IMPROVEMENT DECOCTION Traditional Chinese medicine Scientization
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Research on the development methodology for clinical practice guidelines for organic integration of traditional Chinese medicine and Western medicine
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作者 Ying-Hui Jin Yan-Ping Wang +22 位作者 Ying-Lan Xie Gui-Hua Tian Xiao-Yu Zhang Nan-Nan Shi Ke-Hu Yang Xin Sun Yao-Long Chen Da-Rong Wu Xin-Feng Guo Long Ge Chen Zhao Cheng Lu Yin Jiang Jing Guo Si-Yu Yan Yong-Bo Wang Qiao Huang Xiang-Ying Ren Ying-Yue Rao Yun-Yun Wang Meng-Qian Yuan Xian-Tao Zeng Hong-Cai Shang 《Military Medical Research》 SCIE CAS CSCD 2024年第3期313-322,共10页
Integrated traditional Chinese medicine(TCM)and Western medicine(WM)is a new medical science grounded in the knowledge bases of both TCM and WM,which then forms a unique modern medical system in China.Integrated TCM a... Integrated traditional Chinese medicine(TCM)and Western medicine(WM)is a new medical science grounded in the knowledge bases of both TCM and WM,which then forms a unique modern medical system in China.Integrated TCM and WM has a long history in China,and has made important achievements in the process of clinical diagnosis and treatment.However,the methodological defects in currently published clinical practice guidelines(CPGs)limit its development.The organic integration of TCM and WM is a deeper integration of TCM and WM.To realize the progression of"integration"to"organic integration",a targeted and standardized guideline development methodology is needed.Therefore,the purpose of this study is to establish a standardized development procedure for clinical practice guidelines for the organic integration of TCM and WM to promote the systematic integration of TCM and WM research results into clinical practice guidelines in order to achieve optimal results as the whole is greater than the sum of the parts. 展开更多
关键词 METHODOLOGY Traditional Chinese medicine western medicine Organic integration Clinical practice guidelines(CPGs)
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Environmental Conditions Conducive to the Formation of Multiple Tropical Cyclones over the Western North Pacific
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作者 Yining GU Ruifen ZHAN Xiaomeng LI 《Advances in Atmospheric Sciences》 SCIE CAS CSCD 2024年第10期2027-2042,共16页
There is limited understanding regarding the formation of multiple tropical cyclones(MTCs).This study explores the environmental conditions conducive to MTC formation by objectively determining the atmospheric circula... There is limited understanding regarding the formation of multiple tropical cyclones(MTCs).This study explores the environmental conditions conducive to MTC formation by objectively determining the atmospheric circulation patterns favorable for MTC formation over the western North Pacific.Based on 199 MTC events occurring from June to October 1980–2020,four distinct circulation patterns are identified:the monsoon trough(MT)pattern,accounting for 40.3%of occurrences,the confluence zone(CON)pattern at 26.2%,the easterly wave(EW)pattern at 17.8%,and the monsoon gyre(MG)pattern at 15.7%.The MT pattern mainly arises from the interaction between the subtropical high and the monsoon trough,with MTCs forming along the monsoon trough and its flanks.The CON pattern is affected by the subtropical high,the South Asian high,and the monsoon trough,with MTCs emerging at the confluence zone where the prevailing southwesterly and southeasterly flows converge.The EW pattern is dominated by easterly flows,with MTCs developing along the easterly wave train.MTCs in the MG pattern arise within a monsoon vortex characterized by strong southwesterly flows.A quantitative analysis further indicates that MTC formation in the MT pattern is primarily governed by mid-level vertical velocity and low-level vorticity,while mid-level humidity and vertical velocity are significantly important in the other patterns.The meridional shear and convergence of zonal winds are essential in converting barotropic energy from the basic flows to disturbance kinetic energy,acting as the primary source for eddy kinetic energy growth. 展开更多
关键词 multiple tropical cyclones western North Pacific circulation pattern monsoon trough barotropic energy conversion
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Morphological and molecular description of a new species of sandfly,Sergentomyia(Neophlebotomus)ashwanii sp.nov.(Diptera:Psychodidae)from Western Ghats,India
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作者 Prasanta Saini Harish Kumar Shah +3 位作者 Mathew Jessu Sonia T Tom Anns KP Amju 《Asian Pacific Journal of Tropical Medicine》 SCIE CAS 2024年第5期226-234,共9页
Objective:To report a new species of sandfly,Sergentomyia(Neophlebotomus)ashwanii sp.nov.(Diptera:Psychodidae)from Western Ghats,India.Methods:A systematic sandfly survey was conducted in the Thrissur and Kollam distr... Objective:To report a new species of sandfly,Sergentomyia(Neophlebotomus)ashwanii sp.nov.(Diptera:Psychodidae)from Western Ghats,India.Methods:A systematic sandfly survey was conducted in the Thrissur and Kollam districts of Kerala,India using mechanical aspirators,light and sticky traps,both indoor and outdoor habitats,for a period of one year.Deoxyribonucleic acid barcoding of samples was performed targeting mitochondrial cytochrome oxidase I(COI)gene and sequence generated was subjected to phylogenetic analysis.Results:Sergentomyia(Neophlebotomus)ashwanii,a new sandfly species is recorded and described in this communication.A single row of 10-12 pointed teeth in the cibarium with 4-6 small denticles or fore-teeth are the key characteristics that is distinctive from other members of the subgenus Neophlebotomus.Mitochondrial COI barcode followed by phylogenetic analysis of the nucleotide sequence confirms that specimens of the species belong to the same taxonomic group while the genetic distance(14.2%)with the congeners established it to be a different species.Conclusions:The Western Ghats'being an important biodiversity hotspot and has dearth of systematic entomological surveys on sandflies.The current study tried to fill the void and also report a new sandfly species. 展开更多
关键词 Sergentomyia(Neophlebotomus)ashwanii COI barcode western Ghats Phlebotomine sandflies
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An Ultimate Approach to Retarding the Aging Process by Integrating Traditional Chinese Medicine and Western Medicine
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作者 Kam Ming Ko Hoi Yan Leung 《Chinese Medicine》 CAS 2024年第3期35-48,共14页
Aging and death are unavoidable in life. While immortality may be impossible, many people dream of living a long and healthy life. Throughout history, humans have searched for ways to stay young, but have not found an... Aging and death are unavoidable in life. While immortality may be impossible, many people dream of living a long and healthy life. Throughout history, humans have searched for ways to stay young, but have not found an effective way. This may be because the methods used do not target the causes of aging directly. To address this, we investigated how to delay aging using traditional Chinese medicine (TCM) and Western medicine approaches. In this article, we will explain the causes of aging in the context of TCM and Western medicine and suggest methods to delay it. By integrating TCM and Western medicine, I hope to help everyone age healthily and enjoy a long life. 展开更多
关键词 Aging Traditional Chinese Medicine Zang Xiang System Zheng Qi western Medicine Mitochondria MITOPHAGY Senolytics Glutathione Antioxidant Response
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