Antibacterial action of 4-[p-acetylamino benzenesulfonamido] selenomorpholine (SeB) was studied by microcalorimetry with an LKB-2277 Bioactivity Moni-tor. It was found that SeB had the capacity to inhibit the metaboli...Antibacterial action of 4-[p-acetylamino benzenesulfonamido] selenomorpholine (SeB) was studied by microcalorimetry with an LKB-2277 Bioactivity Moni-tor. It was found that SeB had the capacity to inhibit the metabolic growth of Escherichia coli. But in different sol-vent, the extent of inhibition is different. Removing the effect of solvent, we obtained the half-inhibition conce.ntra-tion(IC50) of SeB from the power-time curves. The value of IC50 of SeB was 1319μg ·mL-1.展开更多
Objective:To assess the in-vitro antihacterial activity and anti-inflammatory activity of orally administered different extracts(Hydro-alcoholic,methanolic,ethyl acetate and hexane)of Rauvolfia tetraphylla(R.tetraphyl...Objective:To assess the in-vitro antihacterial activity and anti-inflammatory activity of orally administered different extracts(Hydro-alcoholic,methanolic,ethyl acetate and hexane)of Rauvolfia tetraphylla(R.tetraphylla)root bark in Carrageetiaii induced acute inflammation in rats.Methods:In-vitro antibacterial activity was evaluated for extracts against four Gram positive and four Gram negative bacteria by using cylinder plate assay.Hydro-alcoholic extract(70%v/v ethanol)at 200,400 and 800 mg/kg doses and methanolic,ethyl acetate and hexane extracts at doses 100,200 and 400 mg/kg were tested for anti-inflammatory activity in Carrageenan induced rat paw oedema model and paw thickness was measured every one hour up to 6 hrs.Results:All extracts of R.tetraphylla root bark showed good zone of inhibition against tested bacterial strains.In Carrageenan induced inflammation model,hydro-alcoholic and methanolic extract of R.tetraphylla root bark at three different doses produced significant(P<0.00l)reduction when compared to vehicle treated control group and hexane,ethyl acetate extracts.Conclusions:In the present study extracts of R.tetraphylla root bark shows good in-vitro antibacterial activity and in-vivo anti-inflammatory activity in rats.展开更多
Objective:To access the in vitro antibacterial activity of Hibiscus rosa-sinensis(H.rosa-sinensis) flower extract against human pathogens.Methods:Antibacterial activity was evaluated by using disc and agar diffusion m...Objective:To access the in vitro antibacterial activity of Hibiscus rosa-sinensis(H.rosa-sinensis) flower extract against human pathogens.Methods:Antibacterial activity was evaluated by using disc and agar diffusion methods.The protein was run through poly acrylmide gel electrophoresis to view their protein profile.Results:The results showed that the cold extraction illustrates a maximum zone of inhibition against Bacillus suhtillis(B.suhtillis),Escherichia coli(E.coli) viz.,(17.00±2.91),(14.50±1.71)mm.followed by hot extraction against.E.coli.Salmonella sp.as(11.66 ±3.14),(10.60±3.09)mm.In methanol extraction showed a highest zone of inhibition recorded against B.suhtillis,E.coli as(18.86±0.18),(18.00±1.63) mm pursued by ethanol extraction shower) utmost zone of inhibition recorded against Salmonella sp.at(20.40±1.54) mm.The crude protein from flower showed a maximum inhibitory zone observed against Salmonella sp.,E.coli viz.,(16.55±1.16),(14.30±2.86)mm.The flower material can be taken as an alternative source of antibacterial agent against the human pathogens.Conclusions:The extracts of the H.rosasinensis art;proved to have potential antibacterial activity,further studies arc highly need for the drug development.展开更多
A new unsymmetrical Schiff base ligand (H2LLi) was synthesized using L-lysine, salicylaldehyde and 2-hydroxy-1-naphthaldehyde. Three solid metal complexes of this ligand \[Ln(H2L)(NO3)\] NO3·2H2O (Ln=La, Sm, Ho) ...A new unsymmetrical Schiff base ligand (H2LLi) was synthesized using L-lysine, salicylaldehyde and 2-hydroxy-1-naphthaldehyde. Three solid metal complexes of this ligand \[Ln(H2L)(NO3)\] NO3·2H2O (Ln=La, Sm, Ho) have been prepared and characterized by elemental analyses, IR spectra, UV spectra, TG-DTG and molar conductance. The antibacterial activities of the ligand and its complexes are also studied. The antibacterial experiments indicate that the ligand and its complexes possess antibacterial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis and that the complexes have higher activity than those of the ligand.展开更多
[Objectives] This study was conducted to determine in-vitro antibacterial effect of 58 Chinese herbs on Staphylococcus aureus,and screen single Chinese herbs with better in-vitro antibacterial effect for formulation o...[Objectives] This study was conducted to determine in-vitro antibacterial effect of 58 Chinese herbs on Staphylococcus aureus,and screen single Chinese herbs with better in-vitro antibacterial effect for formulation of prescription. [Methods]Effective components in Chinese herbs were extracted with water,and inhibition zone diameters and minimal inhibitory concentrations of the 58 Chinese herbs against S. aureus were determined by agar plate punching method and micro two-fold method,respectively. [Results] Twenty two kinds of Chinese herbs including Terminalia chebula Retz.,Anemarrhena asphodeloides Bunge.,Prunella vulgaris L,Rheum palmatum L. and Mosla chinensis Maxim. had better antibacterial effect,among which T. chebula Retz.,A. asphodeloides Bunge.,P. vulgaris L,R. palmatum L. and C. sappan Linn showed the best antibacterial effect,with average inhibition zone over 20 mm and MIC lower than 15. 6 mg/ml.[Conclusions]Traditional Chinese herbs have certain antibacterial effect,and this study will provide important theoretical reference for clinical medication and development of prescription.展开更多
以“草果”“菌”“病毒”“化学成分”“作用机制”“Amomum tsao-ko”“Antibacterial”“Antimicrobial”“Virus”“Chemical composition”“Mechanism”等为关键词,在中国知网、维普数据库、中国专利网、PubMed、Web of Science...以“草果”“菌”“病毒”“化学成分”“作用机制”“Amomum tsao-ko”“Antibacterial”“Antimicrobial”“Virus”“Chemical composition”“Mechanism”等为关键词,在中国知网、维普数据库、中国专利网、PubMed、Web of Science等数据库中组合查询到2011~2023年中的有效文献57篇,对草果抗菌物质基础、抗菌活性与机理、抗病毒活性等方面进行归纳与总结。表明草果具有广谱的抗微生物活性,对细菌、真菌和HSV-1病毒均具有不同程度的体外抑制作用和体内抗感染作用,其中挥发油、酚酸、黄酮及双环壬烷类为草果抗菌物质基础,作用机制主要表现为破坏细胞壁、细胞膜的完整性及抑制病原菌的呼吸代谢过程。但目前对草果抗微生物作用多局限于粗提物的体外抑制,体内各个药物组分之间的相互作用并不明确,且缺乏毒理学和药动学的相关研究,其安全性及药效物质基础尚需进一步深入探索。展开更多
文摘Antibacterial action of 4-[p-acetylamino benzenesulfonamido] selenomorpholine (SeB) was studied by microcalorimetry with an LKB-2277 Bioactivity Moni-tor. It was found that SeB had the capacity to inhibit the metabolic growth of Escherichia coli. But in different sol-vent, the extent of inhibition is different. Removing the effect of solvent, we obtained the half-inhibition conce.ntra-tion(IC50) of SeB from the power-time curves. The value of IC50 of SeB was 1319μg ·mL-1.
文摘Objective:To assess the in-vitro antihacterial activity and anti-inflammatory activity of orally administered different extracts(Hydro-alcoholic,methanolic,ethyl acetate and hexane)of Rauvolfia tetraphylla(R.tetraphylla)root bark in Carrageetiaii induced acute inflammation in rats.Methods:In-vitro antibacterial activity was evaluated for extracts against four Gram positive and four Gram negative bacteria by using cylinder plate assay.Hydro-alcoholic extract(70%v/v ethanol)at 200,400 and 800 mg/kg doses and methanolic,ethyl acetate and hexane extracts at doses 100,200 and 400 mg/kg were tested for anti-inflammatory activity in Carrageenan induced rat paw oedema model and paw thickness was measured every one hour up to 6 hrs.Results:All extracts of R.tetraphylla root bark showed good zone of inhibition against tested bacterial strains.In Carrageenan induced inflammation model,hydro-alcoholic and methanolic extract of R.tetraphylla root bark at three different doses produced significant(P<0.00l)reduction when compared to vehicle treated control group and hexane,ethyl acetate extracts.Conclusions:In the present study extracts of R.tetraphylla root bark shows good in-vitro antibacterial activity and in-vivo anti-inflammatory activity in rats.
文摘Objective:To access the in vitro antibacterial activity of Hibiscus rosa-sinensis(H.rosa-sinensis) flower extract against human pathogens.Methods:Antibacterial activity was evaluated by using disc and agar diffusion methods.The protein was run through poly acrylmide gel electrophoresis to view their protein profile.Results:The results showed that the cold extraction illustrates a maximum zone of inhibition against Bacillus suhtillis(B.suhtillis),Escherichia coli(E.coli) viz.,(17.00±2.91),(14.50±1.71)mm.followed by hot extraction against.E.coli.Salmonella sp.as(11.66 ±3.14),(10.60±3.09)mm.In methanol extraction showed a highest zone of inhibition recorded against B.suhtillis,E.coli as(18.86±0.18),(18.00±1.63) mm pursued by ethanol extraction shower) utmost zone of inhibition recorded against Salmonella sp.at(20.40±1.54) mm.The crude protein from flower showed a maximum inhibitory zone observed against Salmonella sp.,E.coli viz.,(16.55±1.16),(14.30±2.86)mm.The flower material can be taken as an alternative source of antibacterial agent against the human pathogens.Conclusions:The extracts of the H.rosasinensis art;proved to have potential antibacterial activity,further studies arc highly need for the drug development.
文摘A new unsymmetrical Schiff base ligand (H2LLi) was synthesized using L-lysine, salicylaldehyde and 2-hydroxy-1-naphthaldehyde. Three solid metal complexes of this ligand \[Ln(H2L)(NO3)\] NO3·2H2O (Ln=La, Sm, Ho) have been prepared and characterized by elemental analyses, IR spectra, UV spectra, TG-DTG and molar conductance. The antibacterial activities of the ligand and its complexes are also studied. The antibacterial experiments indicate that the ligand and its complexes possess antibacterial activity against Escherichia coli, Staphylococcus aureus and Bacillus subtilis and that the complexes have higher activity than those of the ligand.
基金Supported by Science and Technology Planning Project of Qinhuangdao City(201602A185)
文摘[Objectives] This study was conducted to determine in-vitro antibacterial effect of 58 Chinese herbs on Staphylococcus aureus,and screen single Chinese herbs with better in-vitro antibacterial effect for formulation of prescription. [Methods]Effective components in Chinese herbs were extracted with water,and inhibition zone diameters and minimal inhibitory concentrations of the 58 Chinese herbs against S. aureus were determined by agar plate punching method and micro two-fold method,respectively. [Results] Twenty two kinds of Chinese herbs including Terminalia chebula Retz.,Anemarrhena asphodeloides Bunge.,Prunella vulgaris L,Rheum palmatum L. and Mosla chinensis Maxim. had better antibacterial effect,among which T. chebula Retz.,A. asphodeloides Bunge.,P. vulgaris L,R. palmatum L. and C. sappan Linn showed the best antibacterial effect,with average inhibition zone over 20 mm and MIC lower than 15. 6 mg/ml.[Conclusions]Traditional Chinese herbs have certain antibacterial effect,and this study will provide important theoretical reference for clinical medication and development of prescription.
文摘以“草果”“菌”“病毒”“化学成分”“作用机制”“Amomum tsao-ko”“Antibacterial”“Antimicrobial”“Virus”“Chemical composition”“Mechanism”等为关键词,在中国知网、维普数据库、中国专利网、PubMed、Web of Science等数据库中组合查询到2011~2023年中的有效文献57篇,对草果抗菌物质基础、抗菌活性与机理、抗病毒活性等方面进行归纳与总结。表明草果具有广谱的抗微生物活性,对细菌、真菌和HSV-1病毒均具有不同程度的体外抑制作用和体内抗感染作用,其中挥发油、酚酸、黄酮及双环壬烷类为草果抗菌物质基础,作用机制主要表现为破坏细胞壁、细胞膜的完整性及抑制病原菌的呼吸代谢过程。但目前对草果抗微生物作用多局限于粗提物的体外抑制,体内各个药物组分之间的相互作用并不明确,且缺乏毒理学和药动学的相关研究,其安全性及药效物质基础尚需进一步深入探索。
