Capillary zone electrophoresis (CZE) is a sensitive and rapid technique for determining traces of inorganic cations in water samples. CZE with indirect UV-diode array detection (CZE-DAD) was utilized to identify sever...Capillary zone electrophoresis (CZE) is a sensitive and rapid technique for determining traces of inorganic cations in water samples. CZE with indirect UV-diode array detection (CZE-DAD) was utilized to identify several inorganic cations in natural, potable, and wastewater samples. A pH 4.35 background electrolyte system was employed and consisted of 15 mM imidazole, 8 mM malonic acid, 2 mM 18-crown-6 ether as complexing agents, 10% v/v methanol as an organic modifier with indirect absorbance reference at 214 nm. The CZE method involved electromigration injection at 5 kV for 5 s, a separation voltage of 20 kV at 25℃, and a detection wavelength of 280 nm. Six main cations (ammonium , potassium K+, calcium Ca2+, sodium Na+, magnesium Mg2+, and lead Pb2+) were tested, and all but lead, were detected in the water samples at concentrations between 0.03 and 755 ppm with a detection limit ranging between 0.023 and 0.084 ppm. The successful evaluation of the proposed methodology allowed us to reliably detect and separate six metal ions in different water samples without any pretreatment. All water samples were collected from Northern New York towns and the Raquette River water system, the third longest river in New York State and the largest watershed of the central and western Adirondacks.展开更多
建立了一种利用胶束电动毛细管色谱-间接紫外检测法同时测定丙二酸、甲酸、酒石酸、苹果酸、琥珀酸、戊二酸、乙酸、乳酸和谷氨酸的新方法。以7.5mm o l/L邻苯二甲酸氢钾-1.5mm o l/L十六烷基三甲基溴化铵(用0.1m o l/L氢氧化钠调pH至6....建立了一种利用胶束电动毛细管色谱-间接紫外检测法同时测定丙二酸、甲酸、酒石酸、苹果酸、琥珀酸、戊二酸、乙酸、乳酸和谷氨酸的新方法。以7.5mm o l/L邻苯二甲酸氢钾-1.5mm o l/L十六烷基三甲基溴化铵(用0.1m o l/L氢氧化钠调pH至6.50)混合液作为电泳介质,检测波长为300nm,参比波长为210nm,未涂层弹性石英毛细管(50μm i.d.×64cm)为分离通道,在6m in内实现了9种酸的完全分离。9种有机酸的浓度与其峰面积在一定的范围呈良好的线性关系,检出限均低于1.5m g/L,迁移时间和峰面积的日内、日间相对标准偏差均小于6%。该法用于糖蜜酒精废液中有机酸的测定,结果令人满意,9种有机酸的样品加标回收率均在93%以上。展开更多
文摘Capillary zone electrophoresis (CZE) is a sensitive and rapid technique for determining traces of inorganic cations in water samples. CZE with indirect UV-diode array detection (CZE-DAD) was utilized to identify several inorganic cations in natural, potable, and wastewater samples. A pH 4.35 background electrolyte system was employed and consisted of 15 mM imidazole, 8 mM malonic acid, 2 mM 18-crown-6 ether as complexing agents, 10% v/v methanol as an organic modifier with indirect absorbance reference at 214 nm. The CZE method involved electromigration injection at 5 kV for 5 s, a separation voltage of 20 kV at 25℃, and a detection wavelength of 280 nm. Six main cations (ammonium , potassium K+, calcium Ca2+, sodium Na+, magnesium Mg2+, and lead Pb2+) were tested, and all but lead, were detected in the water samples at concentrations between 0.03 and 755 ppm with a detection limit ranging between 0.023 and 0.084 ppm. The successful evaluation of the proposed methodology allowed us to reliably detect and separate six metal ions in different water samples without any pretreatment. All water samples were collected from Northern New York towns and the Raquette River water system, the third longest river in New York State and the largest watershed of the central and western Adirondacks.
文摘建立了一种利用胶束电动毛细管色谱-间接紫外检测法同时测定丙二酸、甲酸、酒石酸、苹果酸、琥珀酸、戊二酸、乙酸、乳酸和谷氨酸的新方法。以7.5mm o l/L邻苯二甲酸氢钾-1.5mm o l/L十六烷基三甲基溴化铵(用0.1m o l/L氢氧化钠调pH至6.50)混合液作为电泳介质,检测波长为300nm,参比波长为210nm,未涂层弹性石英毛细管(50μm i.d.×64cm)为分离通道,在6m in内实现了9种酸的完全分离。9种有机酸的浓度与其峰面积在一定的范围呈良好的线性关系,检出限均低于1.5m g/L,迁移时间和峰面积的日内、日间相对标准偏差均小于6%。该法用于糖蜜酒精废液中有机酸的测定,结果令人满意,9种有机酸的样品加标回收率均在93%以上。
