Infectious laryngotracheitis(ILT) is an important respiratory disease of chickens and annually causes significant economic losses in the poultry industry worldwide. ILT virus(ILTV) belongs to alphaherpesvirinae and th...Infectious laryngotracheitis(ILT) is an important respiratory disease of chickens and annually causes significant economic losses in the poultry industry worldwide. ILT virus(ILTV) belongs to alphaherpesvirinae and the Gallid herpesvirus 1 species. The transmission of ILTV is via respiratory and ocular routes. Clinical and post-mortem signs of ILT can be separated into two forms according to its virulence. The characteristic of the severe form is bloody mucus in the trachea with high mortality. The mild form causes nasal discharge, conjunctivitis, and reduced weight gain and egg production. Conventional polymerase chain reaction(PCR), nested PCR, real-time PCR, and loop-mediated isothermal amplification were developed to detect ILTV samples from natural or experimentally infected birds. The PCR combined with restriction fragment length polymorphism(RFLP) can separate ILTVs into several genetic groups. These groups can separate vaccine from wild type field viruses. Vaccination is a common method to prevent ILT. However, field isolates and vaccine viruses can establish latent infected carriers. According to PCR-RFLP results, virulent field ILTVs can be derived from modified-live vaccines. Therefore, modified-live vaccine reversion provides a source for ILT outbreaks on chicken farms. Two recently licensed commercial recombinant ILT vaccines are also in use. Other recombinant and gene-deficient vaccine candidates are in the developmental stages. They offer additional hope for the control of this disease. However, in ILT endemic regions, improved biosecurity and management practices are critical for improved ILT control.展开更多
[ Objective] This study aimed to screen Chinese herbal medicines against avian infectious laryngotracheitis virus and Escherichia coli. [ Method ] Conventional Chinese medicine plate dilution method, plate punching me...[ Objective] This study aimed to screen Chinese herbal medicines against avian infectious laryngotracheitis virus and Escherichia coli. [ Method ] Conventional Chinese medicine plate dilution method, plate punching method and test tube method were applied to screen Chinese herbal medicines against avian infectious laryngotracheitis virus based on chicken embryo inoculation experiment. [ Result ] Forsythia suspensa, Radix lsatidis, Isatis iadigotica, Lonicera japonica, Codonopsis pilosula, Astragalus membranaceus, Rhizoma Atractylodis Macrocephalae, C, tyeyrrhiza uralensis and Pericarpium granati had relatively strong anti-ILTV effect; among the Chinese herbal medicines against avian E. coli, Sanguisorba offwinalis, Fructus Mume, Rheum palmatum, Anemarrhena asphodeloides, Radix Scutellariae and Fagopyrum cymosum had relatively strong effect against avian E. coli Os, while other Chinese herbal medicines had relatively weak or no inhibitory effect on avian E. coli 0s and 05. [ Conclusion] This study laid the foundation for further development of Chinese herbal medicine compound preparations to treat avian infectious laryngotracheitis, avian colibacillosis and other viral diseases and bacterial diseases.展开更多
[ Objective] To isolate and identify infectious laryngotracheitis virus （ILTV） from chickens. [ Method] Larynx, trachea, liver and other organs were collected from infectious laryngotracheitis （ILT）-suspected laye...[ Objective] To isolate and identify infectious laryngotracheitis virus （ILTV） from chickens. [ Method] Larynx, trachea, liver and other organs were collected from infectious laryngotracheitis （ILT）-suspected layers. And ILTV TK gene was amplified from these specimens by PCR for initial diagnosis. Virus fluid was isolated and inoculated into SPF chicken embryos via allantoic cavity and chorioallantoic membrane （CAM）, respectively. Hyaluronic acid in allantoic fluid was detected, and CAM lesions were observed. The definite diagnosis was performed through animal regression test. [Result] A 1.3 kbp fragment was amplified from larynx and its secretion of the ILT-suspected chickens. And its amino acid sequence had 98.5% homology to that of ILTV TKgene published in GenBank. After the chicken embryos were inoculated with the isolated ILFV fluid, pox spots, giant polynuclear syncytial cells having intranuclear inclusion bodies were observed in CAM. After being challenged by the IL TV fluid, the chickens showed typical respiratory symptoms and pathological changes of ILT. [Coudusion] A field strain named ILTV XZ09 was isolated from larynx and its secretion of ILT-suspected chickens.展开更多
The secreted alphaherpesvirus glycoprotein G (gG) works differently from other proteins. Analysis of the role of ILTV gG in virus attachment, penetration, direct cell-to-cell spread (CTCS) and the growth curve showed ...The secreted alphaherpesvirus glycoprotein G (gG) works differently from other proteins. Analysis of the role of ILTV gG in virus attachment, penetration, direct cell-to-cell spread (CTCS) and the growth curve showed that gG or its antibody had no effect on ILTV attachment and penetration and that the gG antibody reduced the virus plaque size and the one-step growth curve on chicken embryo liver (CEL) cells, but gG did not affect the virus plaque size or the one-step growth curve on CEL cells. Laser scanning confocal microscopy (LSCM) detection showed that ILTV gG is located in the perinuclear region and the membrane of the CEL cells. These results suggested that ILTV gG might contribute to direct cell-to-cell transmission.展开更多
将重组疫苗在SPF鸡中进行连续传代评估表达ILTV g B和UL32基因的重组鸡痘病毒疫苗的安全性及稳定性。研究结果显示,将重组疫苗在SPF鸡体内连传5代,未观察到接种鸡有不良反应。接种鸡仅短暂排毒且重组病毒在环境中存在时间极短,而同居非...将重组疫苗在SPF鸡中进行连续传代评估表达ILTV g B和UL32基因的重组鸡痘病毒疫苗的安全性及稳定性。研究结果显示,将重组疫苗在SPF鸡体内连传5代,未观察到接种鸡有不良反应。接种鸡仅短暂排毒且重组病毒在环境中存在时间极短,而同居非免疫鸡未被感染,表明重组病毒不具有水平传播能力。免疫后14 d对第1代和第5代接种鸡进行鸡痘强毒株攻击,保护率均在90%以上,表明重组疫苗具有较好的保护力。第5代病毒与第1代病毒相比,UL32基因的序列无任何变异,表明该疫苗具有良好的遗传稳定性,且各代次毒株毒价稳定,无毒力返强现象。以上结果显示,表达ILTV g B和UL32基因的重组鸡痘病毒疫苗具有良好安全性和稳定性。展开更多
文摘Infectious laryngotracheitis(ILT) is an important respiratory disease of chickens and annually causes significant economic losses in the poultry industry worldwide. ILT virus(ILTV) belongs to alphaherpesvirinae and the Gallid herpesvirus 1 species. The transmission of ILTV is via respiratory and ocular routes. Clinical and post-mortem signs of ILT can be separated into two forms according to its virulence. The characteristic of the severe form is bloody mucus in the trachea with high mortality. The mild form causes nasal discharge, conjunctivitis, and reduced weight gain and egg production. Conventional polymerase chain reaction(PCR), nested PCR, real-time PCR, and loop-mediated isothermal amplification were developed to detect ILTV samples from natural or experimentally infected birds. The PCR combined with restriction fragment length polymorphism(RFLP) can separate ILTVs into several genetic groups. These groups can separate vaccine from wild type field viruses. Vaccination is a common method to prevent ILT. However, field isolates and vaccine viruses can establish latent infected carriers. According to PCR-RFLP results, virulent field ILTVs can be derived from modified-live vaccines. Therefore, modified-live vaccine reversion provides a source for ILT outbreaks on chicken farms. Two recently licensed commercial recombinant ILT vaccines are also in use. Other recombinant and gene-deficient vaccine candidates are in the developmental stages. They offer additional hope for the control of this disease. However, in ILT endemic regions, improved biosecurity and management practices are critical for improved ILT control.
基金Supported by Project of Science and Technology Department of Hebei Province(08820412D)Project of Qinghuangdao Municipal Science and Technology Bureau[QKJ(2003)No.30-35]+1 种基金Project of Shijiazhuang Municipal Science and Technology Bureau(07150193A)Scientific Research Innovation Team of Hebei Normal University of Science and Technology(CXTD201201)
文摘[ Objective] This study aimed to screen Chinese herbal medicines against avian infectious laryngotracheitis virus and Escherichia coli. [ Method ] Conventional Chinese medicine plate dilution method, plate punching method and test tube method were applied to screen Chinese herbal medicines against avian infectious laryngotracheitis virus based on chicken embryo inoculation experiment. [ Result ] Forsythia suspensa, Radix lsatidis, Isatis iadigotica, Lonicera japonica, Codonopsis pilosula, Astragalus membranaceus, Rhizoma Atractylodis Macrocephalae, C, tyeyrrhiza uralensis and Pericarpium granati had relatively strong anti-ILTV effect; among the Chinese herbal medicines against avian E. coli, Sanguisorba offwinalis, Fructus Mume, Rheum palmatum, Anemarrhena asphodeloides, Radix Scutellariae and Fagopyrum cymosum had relatively strong effect against avian E. coli Os, while other Chinese herbal medicines had relatively weak or no inhibitory effect on avian E. coli 0s and 05. [ Conclusion] This study laid the foundation for further development of Chinese herbal medicine compound preparations to treat avian infectious laryngotracheitis, avian colibacillosis and other viral diseases and bacterial diseases.
文摘[ Objective] To isolate and identify infectious laryngotracheitis virus （ILTV） from chickens. [ Method] Larynx, trachea, liver and other organs were collected from infectious laryngotracheitis （ILT）-suspected layers. And ILTV TK gene was amplified from these specimens by PCR for initial diagnosis. Virus fluid was isolated and inoculated into SPF chicken embryos via allantoic cavity and chorioallantoic membrane （CAM）, respectively. Hyaluronic acid in allantoic fluid was detected, and CAM lesions were observed. The definite diagnosis was performed through animal regression test. [Result] A 1.3 kbp fragment was amplified from larynx and its secretion of the ILT-suspected chickens. And its amino acid sequence had 98.5% homology to that of ILTV TKgene published in GenBank. After the chicken embryos were inoculated with the isolated ILFV fluid, pox spots, giant polynuclear syncytial cells having intranuclear inclusion bodies were observed in CAM. After being challenged by the IL TV fluid, the chickens showed typical respiratory symptoms and pathological changes of ILT. [Coudusion] A field strain named ILTV XZ09 was isolated from larynx and its secretion of ILT-suspected chickens.
文摘The secreted alphaherpesvirus glycoprotein G (gG) works differently from other proteins. Analysis of the role of ILTV gG in virus attachment, penetration, direct cell-to-cell spread (CTCS) and the growth curve showed that gG or its antibody had no effect on ILTV attachment and penetration and that the gG antibody reduced the virus plaque size and the one-step growth curve on chicken embryo liver (CEL) cells, but gG did not affect the virus plaque size or the one-step growth curve on CEL cells. Laser scanning confocal microscopy (LSCM) detection showed that ILTV gG is located in the perinuclear region and the membrane of the CEL cells. These results suggested that ILTV gG might contribute to direct cell-to-cell transmission.
文摘将重组疫苗在SPF鸡中进行连续传代评估表达ILTV g B和UL32基因的重组鸡痘病毒疫苗的安全性及稳定性。研究结果显示,将重组疫苗在SPF鸡体内连传5代,未观察到接种鸡有不良反应。接种鸡仅短暂排毒且重组病毒在环境中存在时间极短,而同居非免疫鸡未被感染,表明重组病毒不具有水平传播能力。免疫后14 d对第1代和第5代接种鸡进行鸡痘强毒株攻击,保护率均在90%以上,表明重组疫苗具有较好的保护力。第5代病毒与第1代病毒相比,UL32基因的序列无任何变异,表明该疫苗具有良好的遗传稳定性,且各代次毒株毒价稳定,无毒力返强现象。以上结果显示,表达ILTV g B和UL32基因的重组鸡痘病毒疫苗具有良好安全性和稳定性。
