OBJECTIVE: To investigate the effect of Shenfuqiangxin capsule and the underlying mechanism on cardio-renal syndrome(CRS) in rats induced by infrarenal aortic-clamping after renal ischaemia.METHODS: Male Wistar rats u...OBJECTIVE: To investigate the effect of Shenfuqiangxin capsule and the underlying mechanism on cardio-renal syndrome(CRS) in rats induced by infrarenal aortic-clamping after renal ischaemia.METHODS: Male Wistar rats underwent infrarenal aortic-clamping after renal ischaemia or sham operation. The surviving CRS rats were divided randomly into three groups: CRS group(CRS + 10 m L·kg-1·d-1pure water by gavage), SFQX group(CRS + 13.2 g crude drug·kg- 1·d- 1Shenfuqiangxin by gavage),and handleregionpeptide(HRP)group(CRS+10mg/kg HRP by vein). Sham operation rats were given10 m L·kg-1·d-1pure water. Treatments were given8 weeks after surgery, which lasted for 4 weeks. Therats were detected for heart structure and function by transthoracic echocardiography. PPR m RNA was detected by Reverse Transcription Polymerase Chain Reaction(RT-PCR). To determine whether the mitogen-activated protein kinase(MAPK) signal pathway is included in the heart and kidney protective function of Shenfuqiangxin capsule, MAPK related proteins such as posophorylated C-Jun amino terminal kinase(p-JNK), posophorylated extracellular signal-regulated kinase ?(p-ERK1/2), posophorylated p38(p-p38) were examined by Western Blot.Apoptosis in heart and kidney tissues were detected by d UTP Nick End Labeling staining.RESULTS: Shenfuqiangxin capsule alleviated myocardial apoptosis and inhibited PRR m RNA expression and p-JNK, p-ERK1/2, p-p38 proteins expression in CRS rats.CONCLUSION: All the results suggest that Shenfuqiangxin capsule improves the injured heart and kidney function maybe through inhibition of MAPK response pathway.展开更多
目的:观察大鼠肾下腹主动脉缺血再灌注对心肌肌浆网钙泵(sarcoplasmic reticulum Ca2+-ATPase,SERCA)的活性及其基因表达的影响。方法:复制大鼠肾下腹主动脉缺血再灌注(ischemia and reperfusion,I/R)模型。将SD雄性大鼠随机分为假手术...目的:观察大鼠肾下腹主动脉缺血再灌注对心肌肌浆网钙泵(sarcoplasmic reticulum Ca2+-ATPase,SERCA)的活性及其基因表达的影响。方法:复制大鼠肾下腹主动脉缺血再灌注(ischemia and reperfusion,I/R)模型。将SD雄性大鼠随机分为假手术组,缺血30min分别再灌注2、12、24和48h(I/R2h组、I/R12h组、I/R24h组、I/R48h组)。采用无机磷比色法检测缺血再灌注不同时间点心肌肌浆网SERCA活性,反转录酶PCR技术测定SERCA基因的表达。结果:I/R24h组、I/R48h组SERCA的活性及基因表达均显著低于假手术组(P<0.01);I/R12h组SERCA的活性均低于假手术组(P<0.01),但基因表达无明显变化(P>0.05);I/R2h组SERCA的活性及其基因表达与假手术组差异均无统计学意义(P>0.05)。结论:肾下腹主动脉缺血再灌注过程严重影响SERCA的活性及其基因的表达。展开更多
目的:根据2018国际妇产科联盟(FIGO)剖宫产时胎盘植入性疾病(PAS)临床分级系统,评估腹主动脉球囊预置术在凶险性前置胎盘(PPP)伴不同分级PAS剖宫产术中的应用价值。方法:回顾性分析2014年1月至2020年12月在上海交通大学医学院附属国际...目的:根据2018国际妇产科联盟(FIGO)剖宫产时胎盘植入性疾病(PAS)临床分级系统,评估腹主动脉球囊预置术在凶险性前置胎盘(PPP)伴不同分级PAS剖宫产术中的应用价值。方法:回顾性分析2014年1月至2020年12月在上海交通大学医学院附属国际和平妇幼保健院产科住院并剖宫产分娩的230例PPP合并PAS产妇的临床资料,剖宫产前行腹主动脉球囊预置术后为观察组(160例),未行预置术的为对照组(70例),根据2018年FIGO的PAS分级标准进一步分组,比较不同PAS分级标准的两组患者术中、术后及并发症等情况。结果:(1)PAS分级2级、PAS分级3级中观察组与对照组的手术时间、术中出血量、术中输血、术后血红蛋白的下降、子宫切除率比较,差异均无统计学意义(P>0.05)。而PAS分级4~5级中观察组孕妇术中出血量明显少于对照组(3533.3±2391.4 ml vs 4293.6±1235.4 ml,P<0.05),且术中全子宫切除率低于对照组(5.0%vs 33.3%,P<0.05)。(2)两组产妇术后入住ICU时间、术后住院时间、术后抗生素使用时间、术后病率差异无统计学意义(P>0.05)。(3)两组新生儿出生体质量、Apgar评分<7分、新生儿住院率比较,差异均无统计学意义(P>0.05)。结论:术前联合超声检查及MRI检查充分评估,经多学科协作,对于PAS分级4~5级及以上水平患者建议术前行腹主动脉球囊预置术,以期望减少术中出血量并降低子宫切除率。展开更多
基金Natural Science Foundation-funded Project:Study on Mechanism of Invigorate Qi Warm Yang Activate Blood And Promote Dieresis Treatment Adjusted By MAPK Mediated by(P)RR(No.30973795)Sponsored by the the National Natural Science Foundation of China(No.81202801)
文摘OBJECTIVE: To investigate the effect of Shenfuqiangxin capsule and the underlying mechanism on cardio-renal syndrome(CRS) in rats induced by infrarenal aortic-clamping after renal ischaemia.METHODS: Male Wistar rats underwent infrarenal aortic-clamping after renal ischaemia or sham operation. The surviving CRS rats were divided randomly into three groups: CRS group(CRS + 10 m L·kg-1·d-1pure water by gavage), SFQX group(CRS + 13.2 g crude drug·kg- 1·d- 1Shenfuqiangxin by gavage),and handleregionpeptide(HRP)group(CRS+10mg/kg HRP by vein). Sham operation rats were given10 m L·kg-1·d-1pure water. Treatments were given8 weeks after surgery, which lasted for 4 weeks. Therats were detected for heart structure and function by transthoracic echocardiography. PPR m RNA was detected by Reverse Transcription Polymerase Chain Reaction(RT-PCR). To determine whether the mitogen-activated protein kinase(MAPK) signal pathway is included in the heart and kidney protective function of Shenfuqiangxin capsule, MAPK related proteins such as posophorylated C-Jun amino terminal kinase(p-JNK), posophorylated extracellular signal-regulated kinase ?(p-ERK1/2), posophorylated p38(p-p38) were examined by Western Blot.Apoptosis in heart and kidney tissues were detected by d UTP Nick End Labeling staining.RESULTS: Shenfuqiangxin capsule alleviated myocardial apoptosis and inhibited PRR m RNA expression and p-JNK, p-ERK1/2, p-p38 proteins expression in CRS rats.CONCLUSION: All the results suggest that Shenfuqiangxin capsule improves the injured heart and kidney function maybe through inhibition of MAPK response pathway.
文摘目的:观察大鼠肾下腹主动脉缺血再灌注对心肌肌浆网钙泵(sarcoplasmic reticulum Ca2+-ATPase,SERCA)的活性及其基因表达的影响。方法:复制大鼠肾下腹主动脉缺血再灌注(ischemia and reperfusion,I/R)模型。将SD雄性大鼠随机分为假手术组,缺血30min分别再灌注2、12、24和48h(I/R2h组、I/R12h组、I/R24h组、I/R48h组)。采用无机磷比色法检测缺血再灌注不同时间点心肌肌浆网SERCA活性,反转录酶PCR技术测定SERCA基因的表达。结果:I/R24h组、I/R48h组SERCA的活性及基因表达均显著低于假手术组(P<0.01);I/R12h组SERCA的活性均低于假手术组(P<0.01),但基因表达无明显变化(P>0.05);I/R2h组SERCA的活性及其基因表达与假手术组差异均无统计学意义(P>0.05)。结论:肾下腹主动脉缺血再灌注过程严重影响SERCA的活性及其基因的表达。
文摘目的:根据2018国际妇产科联盟(FIGO)剖宫产时胎盘植入性疾病(PAS)临床分级系统,评估腹主动脉球囊预置术在凶险性前置胎盘(PPP)伴不同分级PAS剖宫产术中的应用价值。方法:回顾性分析2014年1月至2020年12月在上海交通大学医学院附属国际和平妇幼保健院产科住院并剖宫产分娩的230例PPP合并PAS产妇的临床资料,剖宫产前行腹主动脉球囊预置术后为观察组(160例),未行预置术的为对照组(70例),根据2018年FIGO的PAS分级标准进一步分组,比较不同PAS分级标准的两组患者术中、术后及并发症等情况。结果:(1)PAS分级2级、PAS分级3级中观察组与对照组的手术时间、术中出血量、术中输血、术后血红蛋白的下降、子宫切除率比较,差异均无统计学意义(P>0.05)。而PAS分级4~5级中观察组孕妇术中出血量明显少于对照组(3533.3±2391.4 ml vs 4293.6±1235.4 ml,P<0.05),且术中全子宫切除率低于对照组(5.0%vs 33.3%,P<0.05)。(2)两组产妇术后入住ICU时间、术后住院时间、术后抗生素使用时间、术后病率差异无统计学意义(P>0.05)。(3)两组新生儿出生体质量、Apgar评分<7分、新生儿住院率比较,差异均无统计学意义(P>0.05)。结论:术前联合超声检查及MRI检查充分评估,经多学科协作,对于PAS分级4~5级及以上水平患者建议术前行腹主动脉球囊预置术,以期望减少术中出血量并降低子宫切除率。
