Standard parenteral nutrition solutions are mixtures comprising interacting components that may degrade themselves over time. The objective of this study was to investigate the physicochemical and microbiological stab...Standard parenteral nutrition solutions are mixtures comprising interacting components that may degrade themselves over time. The objective of this study was to investigate the physicochemical and microbiological stability of a hospital preparation for parenteral nutrition in neonatology. The analyses were performed throughout the storage of the preparations at 2–8 °C(up to 4 months). The extent of stability was based on the determination of amino acids dosage, visual and physicochemical properties(glucose and electrolytes concentrations, pH and osmolality measurements, particle counting) and microbiological analysis(sterility test). A thermal degradation of ascorbic acid was conducted to evaluate the antioxidant properties of the parenteral mixture. Physicochemical and microbiological controls were found to comply with the specifications. Amino acids showed a good stability throughout the 4 months storage except for cysteine, which was progressively degraded to cystine, conferring a yellow coloration to parenteral solutions. Parenteral nutrition standards solutions remain stable for 4 months at 2–8 °C,ensuring safe administration in preterm infants.展开更多
The stabilities of two kinds of solutions (30 mg/mL) of Ampicillin sodium in 0.9% NaCl in water (NS, normal saline) and in sterile water (SW) in the intravenous elastomeric infusion device (Accufuser®) were ev...The stabilities of two kinds of solutions (30 mg/mL) of Ampicillin sodium in 0.9% NaCl in water (NS, normal saline) and in sterile water (SW) in the intravenous elastomeric infusion device (Accufuser®) were evaluated based on recommended solutions and storage periods. The injectable NS- and SW-Ampicillin solutions in the Accufuser® device were stored and evaluated at controlled temperature (room temperature, 25℃ ± 2℃ and cold temperature, 4℃ ± 2℃) during 7 days. Effects of the periods of storage (from 0 to 7 days) and the temperatures of storage (RT and CT) on the physico-chemical appearances and concentrations of active compounds were determined. The visual clarity, pH, and concentrations of Ampicillin were determined by stability-indicating high-performance liquid chromatography (HPLC)-ultraviolet (UV) detection. The results showed that the amount of Ampicillin in studied solutions gradually decreased with time. The Ampicillin in NS, which was stored in CT, was relatively stable, retaining 94% of its original amount up to 7 days. The solution that showed least stability was Ampicillin in SW, which was stored in RT, retaining 80% of its original amount. Generally, solutions that were stored in CT were more stable than the solutions that were stored in RT. No significant changes in physical appearance or color of the solutions were observed during the study. Particles were not detected in any solution samples. In summary, two kinds of solutions of Ampicillin sodium, in NS and SW, showed different chemical stabilities with time in intravenous infusion device without any significant physical changes and retained about 94% vs 89% and 83% vs 80% of initial concentrations after 7 days in CT and RT, respectively. We suggest that 30 mg/mL of Ampicillin sodium in NS solution in an Accufuser® infusion device which is stored in CT can be applicable for 7 days in clinical situations.展开更多
The N-glycine constant infusion method was used to study protein turnover in normal adults and perioperative patients.The protein turnover rate (Q),synthetic rate (S) and catabolic rate (C) were derived according to P...The N-glycine constant infusion method was used to study protein turnover in normal adults and perioperative patients.The protein turnover rate (Q),synthetic rate (S) and catabolic rate (C) were derived according to Picou's two-pool system model,and the“end-product averaging technique”showed the ^(15)N-glycine constant iufusion method to be reliable,with a reproducibility of ±5.1%.This measurement can be repeated after a short period,so it should be useful for surgical nutrition study. Our results show the following:Q was significantly increased after operation (3.4± 0.6 to 5.0±0.5 g protein/kg/d,P<0.01),and S (2.6±0.5 to 3.7±0.6 g protein/kg/d,P<0.05) and C (2.3±0.5 to 3.9±0.5 g protein/kg/d,P<0.01) were increased as well.This suggests that the protein degradation rate outstripped the synthesis rate on post-operative day three (POD+3) when traditional (glutamine-free) total parenteral nutrition support was used.展开更多
A physically accurate and computationally effective pure finite element method （FEM） was developed to simulate the isothermal resin infusing process. The FEM was based on conservation of resin muss at and instant of...A physically accurate and computationally effective pure finite element method （FEM） was developed to simulate the isothermal resin infusing process. The FEM was based on conservation of resin muss at and instant of time and was objective of resin film infusion （RFI） fiber impregnation and mold filling . The developed computer code was able to simulate the resin infusing visually. A numerical example presented here demonstrated that compared with traditional finite element/ control-volume （FE/CV）, and FEM was physically accurate and computationally efficient.展开更多
文摘Standard parenteral nutrition solutions are mixtures comprising interacting components that may degrade themselves over time. The objective of this study was to investigate the physicochemical and microbiological stability of a hospital preparation for parenteral nutrition in neonatology. The analyses were performed throughout the storage of the preparations at 2–8 °C(up to 4 months). The extent of stability was based on the determination of amino acids dosage, visual and physicochemical properties(glucose and electrolytes concentrations, pH and osmolality measurements, particle counting) and microbiological analysis(sterility test). A thermal degradation of ascorbic acid was conducted to evaluate the antioxidant properties of the parenteral mixture. Physicochemical and microbiological controls were found to comply with the specifications. Amino acids showed a good stability throughout the 4 months storage except for cysteine, which was progressively degraded to cystine, conferring a yellow coloration to parenteral solutions. Parenteral nutrition standards solutions remain stable for 4 months at 2–8 °C,ensuring safe administration in preterm infants.
基金supported by Division of Molecular Therapeutics Development,Hanyang Biomedical Research Institute,Hanyang University and Woo Young Medical Co.LTD.,Seoul,South Korea.
文摘The stabilities of two kinds of solutions (30 mg/mL) of Ampicillin sodium in 0.9% NaCl in water (NS, normal saline) and in sterile water (SW) in the intravenous elastomeric infusion device (Accufuser®) were evaluated based on recommended solutions and storage periods. The injectable NS- and SW-Ampicillin solutions in the Accufuser® device were stored and evaluated at controlled temperature (room temperature, 25℃ ± 2℃ and cold temperature, 4℃ ± 2℃) during 7 days. Effects of the periods of storage (from 0 to 7 days) and the temperatures of storage (RT and CT) on the physico-chemical appearances and concentrations of active compounds were determined. The visual clarity, pH, and concentrations of Ampicillin were determined by stability-indicating high-performance liquid chromatography (HPLC)-ultraviolet (UV) detection. The results showed that the amount of Ampicillin in studied solutions gradually decreased with time. The Ampicillin in NS, which was stored in CT, was relatively stable, retaining 94% of its original amount up to 7 days. The solution that showed least stability was Ampicillin in SW, which was stored in RT, retaining 80% of its original amount. Generally, solutions that were stored in CT were more stable than the solutions that were stored in RT. No significant changes in physical appearance or color of the solutions were observed during the study. Particles were not detected in any solution samples. In summary, two kinds of solutions of Ampicillin sodium, in NS and SW, showed different chemical stabilities with time in intravenous infusion device without any significant physical changes and retained about 94% vs 89% and 83% vs 80% of initial concentrations after 7 days in CT and RT, respectively. We suggest that 30 mg/mL of Ampicillin sodium in NS solution in an Accufuser® infusion device which is stored in CT can be applicable for 7 days in clinical situations.
文摘The N-glycine constant infusion method was used to study protein turnover in normal adults and perioperative patients.The protein turnover rate (Q),synthetic rate (S) and catabolic rate (C) were derived according to Picou's two-pool system model,and the“end-product averaging technique”showed the ^(15)N-glycine constant iufusion method to be reliable,with a reproducibility of ±5.1%.This measurement can be repeated after a short period,so it should be useful for surgical nutrition study. Our results show the following:Q was significantly increased after operation (3.4± 0.6 to 5.0±0.5 g protein/kg/d,P<0.01),and S (2.6±0.5 to 3.7±0.6 g protein/kg/d,P<0.05) and C (2.3±0.5 to 3.9±0.5 g protein/kg/d,P<0.01) were increased as well.This suggests that the protein degradation rate outstripped the synthesis rate on post-operative day three (POD+3) when traditional (glutamine-free) total parenteral nutrition support was used.
基金Funded bythe National Natural Science Foundation of China(No50573060)
文摘A physically accurate and computationally effective pure finite element method （FEM） was developed to simulate the isothermal resin infusing process. The FEM was based on conservation of resin muss at and instant of time and was objective of resin film infusion （RFI） fiber impregnation and mold filling . The developed computer code was able to simulate the resin infusing visually. A numerical example presented here demonstrated that compared with traditional finite element/ control-volume （FE/CV）, and FEM was physically accurate and computationally efficient.
