Construction of the Demonstration Area for the Inheritance and Development of Yimeng Red Culture Based on Digital Empowerment

Yimeng red culture is an important part of the excellent culture of the Chinese nation.It is of great practical significance and long-term strategic significance to build the demonstration area for the inheritance and development of Yimeng red culture.Combining digital empowerment with the inheritance and development of Yimeng red culture is a new idea that conforms to the development of the times.In this paper,on the basis of combing Yimeng red cultural resources and combining with the current situation of inheritance and development of Yimeng red culture,the strategic layout and implementation approach of the demonstration area for the inheritance and development of Yimeng red culture were put forward from the perspective of digital empowerment.

Speech at the Meeting on Cultural Inheritance and Development

Today,we convened a meeting on cultural inheritance and development.Preceding this event,I visited the newly built China National Archives of Publications and Culture and the Chinese Archaeological Museum at the Chinese Academy of History and found them exceptionally insightful.

Cytological Mechanism of Cytoplasmic Inheritance in Pinus tabulaeformis: Ⅱ. Transmission of Male and Female Organelles During Fertilization and Proembryo Development

In an earlier report the ultrastructure and nucleoid organelles of male gamete in Pinus tabulaeformis Carr. have been described. Presently, the ultrastructure of the cytoplasm of the egg cell and pollen tube—immediately before fertilization and during cytoplasmic transmission of male gametophyte—has been described for the same species. The fate of parental plastids and mitochondria in the proembryo has also been followed. The mature egg cell contains a large amount of mitochondria, but seems to lack normal plastids. Most plastids have transformed into large inclusions. Apart from the large inclusions, there are abundant small inclusions and other organelles in the egg cell. During fertilization, pollen tube penetrates into the egg cell at the micropylar end and thereafter the contents are released. Plastid and mitochondrion of male origin are lacking near the fusing sperm_egg nuclei. The second sperm nucleus—not involved in karyogamy—remains at a site near the receptive vacuole. This nucleus is surrounded by large amount of male cytoplasm containing mixed organelles from the sperm cell, tube cell, and egg cell. At the free nuclear proembryo stage, organelles of male and female origin are visible in the perinucleus_cytoplasmic zone. Most of the mitochondria have the same morphological features as those in the egg cell. Some of the mitochondria appear to have originated from the sperm and tube cells. Plastids are most likely of male gametophyte origin because they have similar appearance as those of the sperm and tube cell. Large inclusions in the egg cell become vacuole_like. Paternal plastids have been incorporated into the neocytoplasm of the proembryo. In the cellular proembryo, maternal mitochondria are more abundant. Plastids resembling those of the sperm and tube cell are still present. These cytological results clearly show that in P. tabulaeformis, plastids are inherited paternally and mitochondria bipaternally. The cytological mechanism of plastid and mitochondrion inheritance in gymnosperm is discussed.

New aspects of a small GTPase RAB35 in brain development and function

In eukaryotic cells,organelles in the secretory,lysosomal,and endocytic pathways actively exchange biological materials with each other through intracellular membrane trafficking,which is the process of transporting the cargo of proteins,lipids,and other molecules to appropriate compartments via transport vesicles or intermediates.These processes are strictly regulated by various small GTPases such as the RAS-like in rat brain(RAB)protein family,which is the largest subfamily of the RAS superfamily.Dysfunction of membrane trafficking affects tissue homeostasis and leads to a wide range of diseases,including neurological disorders and neurodegenerative diseases.Therefore,it is important to understand the physiological and pathological roles of RAB proteins in brain function.RAB35,a member of the RAB family,is an evolutionarily conserved protein in metazoans.A wide range of studies using cultured mammalian cells and model organisms have revealed that RAB35 mediates various processes such as cytokinesis,endocytic recycling,actin bundling,and cell migration.RAB35 is also involved in neurite outgrowth and turnover of synaptic vesicles.We generated brain-specific Rab35 knockout mice to study the physiological roles of RAB35 in brain development and function.These mice exhibited defects in anxiety-related behaviors and spatial memory.Strikingly,RAB35 is required for the precise positioning of pyramidal neurons during hippocampal development,and thereby for normal hippocampal lamination.In contrast,layer formation in the cerebral cortex occurred superficially,even in the absence of RAB35,suggesting a predominant role for RAB35 in hippocampal development rather than in cerebral cortex development.Recent studies have suggested an association between RAB35 and neurodegenerative diseases,including Parkinson's disease and Alzheimer's disease.In this review,we provide an overview of the current understanding of subcellular functions of RAB35.We also provide insights into the physiological role of RAB35 in mammalian brain development and function,and discuss the involvement of RAB35 dysfunction in neurodegenerative diseases.

Establishment of human cerebral organoid systems to model early neural development and assess the central neurotoxicity of environmental toxins

Human brain development is a complex process,and animal models often have significant limitations.To address this,researchers have developed pluripotent stem cell-derived three-dimensional structures,known as brain-like organoids,to more accurately model early human brain development and disease.To enable more consistent and intuitive reproduction of early brain development,in this study,we incorporated forebrain organoid culture technology into the traditional unguided method of brain organoid culture.This involved embedding organoids in matrigel for only 7 days during the rapid expansion phase of the neural epithelium and then removing them from the matrigel for further cultivation,resulting in a new type of human brain organoid system.This cerebral organoid system replicated the temporospatial characteristics of early human brain development,including neuroepithelium derivation,neural progenitor cell production and maintenance,neuron differentiation and migration,and cortical layer patterning and formation,providing more consistent and reproducible organoids for developmental modeling and toxicology testing.As a proof of concept,we applied the heavy metal cadmium to this newly improved organoid system to test whether it could be used to evaluate the neurotoxicity of environmental toxins.Brain organoids exposed to cadmium for 7 or 14 days manifested severe damage and abnormalities in their neurodevelopmental patterns,including bursts of cortical cell death and premature differentiation.Cadmium exposure caused progressive depletion of neural progenitor cells and loss of organoid integrity,accompanied by compensatory cell proliferation at ectopic locations.The convenience,flexibility,and controllability of this newly developed organoid platform make it a powerful and affordable alternative to animal models for use in neurodevelopmental,neurological,and neurotoxicological studies.

Postnatal development of rat retina:a continuous observation and comparison between the organotypic retinal explant model and in vivo development

The organotypic retinal explant culture has been established for more than a decade and offers a range of unique advantages compared with in vivo experiments and cell cultures.However,the lack of systematic and continuous comparison between in vivo retinal development and the organotypic retinal explant culture makes this model controversial in postnatal retinal development studies.Thus,we aimed to verify the feasibility of using this model for postnatal retinal development studies by comparing it with the in vivo retina.In this study,we showed that postnatal retinal explants undergo normal development,and exhibit a consistent structure and timeline with retinas in vivo.Initially,we used SOX2 and PAX6 immunostaining to identify retinal progenitor cells.We then examined cell proliferation and migration by immunostaining with Ki-67 and doublecortin,respectively.Ki-67-and doublecortin-positive cells decreased in both in vivo and explants during postnatal retinogenesis,and exhibited a high degree of similarity in abundance and distribution between groups.Additionally,we used Ceh-10 homeodomain-containing homolog,glutamate-ammonia ligase(glutamine synthetase),neuronal nuclei,and ionized calcium-binding adapter molecule 1 immunostaining to examine the emergence of bipolar cells,Müller glia,mature neurons,and microglia,respectively.The timing and spatial patterns of the emergence of these cell types were remarkably consistent between in vivo and explant retinas.Our study showed that the organotypic retinal explant culture model had a high degree of consistency with the progression of in vivo early postnatal retina development.The findings confirm the accuracy and credibility of this model and support its use for long-term,systematic,and continuous observation.

Inheritance of Powdery Mildew Resistance in Cucumber (Cucumis sativus L.) and Development of an AFLP Marker for Resistance Detection

Cucumber powdery mildew is one of the most destructive diseases of cucumber throughout the world. In the present study, inheritance of powdery mildew resistance in three crosses, and linkage of resistance with amplified fragment length polymorphism （AFLP） markers are studied to formulate efficient strategies for breeding cultivars resistant to powdery mildew. The joint analysis of multiple generations and AFLP technique has been applied in this study. The best model is the one with two major genes, additive, dominant, and epistatic effects, plus polygenes with additive, dominant, and epistatic effects （E-l-0 model）. The heritabilities of the major genes varied from 64.26% to 97.82%, and susceptibility was incompletely dominant for the two major genes in the three crosses studied. The additive effects of the two major genes and the dominant effect of the second major gene were high, and the epistatic effect of the additive-dominant between the two major genes was the highest in cross I . In cross II, the absolute value of the additive effect, dominant effect, and potential ratio of the first major gene were far higher than those of the second major gene, and the epistatic effect of the additive-additive was the highest. The genetic parameters of the two major genes in cross III were similar to those in cross II. Correlation and regression analyses showed that marker E25/M63-103 was linked to a susceptible gene controlling powdery mildew resistance. The marker could account for 19.98% of the phenotypic variation. When the marker was tested on a diverse set of 29 cucumber lines, the correlation between phenotype and genotype was not significant, which suggested cultivar specialty of gene expression or different methods of resistance to powdery mildew. The target DNA fragment was 103 bp in length, and only a small part was found to be homologous to DNA in the other species evaluated, which indicated that it was unique to the cucumber genome.

Inheritance,Innovation and High-quality Development of Tea Industry in Xianning City,Hubei Province

Xianning City is a main tea producing area in Hubei Province,the capital of"border-selling tea"in China,and an important starting point for the Ten-Thousand-Mile Tea Road across the Eurasian continent.This paper studies the tea industry in Xianning from the aspects of top 100 counties of tea industry,important agricultural cultural heritage,famous Chinese historical and cultural villages,China’s time-honored brands,major brands,and designated production enterprises of border-selling tea.Besides,it introduces intellectual property resources of the tea industry from traditional knowledge,biological genetic resources,new plant varieties,patents,geographical indications,trademarks,etc.Then,it analyzes major problems in the inheritance,innovation and high-quality development of Xianning tea,especially the border-selling tea.Finally,it comes up with constructive recommendations of starting the cultural inheritance and development project and upgrade project of the tea industry to build the capital of China’s border-selling tea.

Inheritance,Innovation and Development of Wuling Mountain Area in Hubei Province Based on Intellectual Property Rights

Using the methods of field survey and literature collection,this paper firstly introduces the intellectual property resources of the Wuling Mountain Area in Hubei Province from folk literature and arts,traditional knowledge,biological genetic resources,new plant varieties,patents,geographical indications,and trademarks.Then,it analyzes the main problems in the protection and inheritance,innovation and development of intellectual property rights(IPRs).In addition,it discusses the strategy of inheritance,innovation and high-quality development of this ethnic area in the context of the strategy of strengthening the country with intellectual property rights and the strategy of rural revitalization.Finally,it comes up with recommendations including carrying forward and inheriting folk literature and art,protecting traditional knowledge of traditional craftsmanship,inheriting and innovating to develop traditional Chinese medicine and ethnic medicine,improving the protection and use of geographical indications,and strengthening the IPR protection.

Study on the Inheritance,Innovation and Development of Scutellaria baicalensis Georgi in the Treatment of COVID-19

The discovery of highly effective medicine"Qingfei Paidu Decoction"for COVID-19 is a new era gift of traditional Chinese medicine to the world.The traditional Chinese medicine Scutellaria baicalensis Georgi,as a medicinal plant in the family of Labiatae,is the dominant variety of genuine medicinal material in North China.It is the leading traditional Chinese medicine in the advantageous area of agricultural products with Chinese characteristics in Shangdang of Changzhi City,and it is one of the 21 herbal medicines in the new national prescription"Qingfei Paidu Decoction"for the rapid and effective treatment of COVID-19.This paper introduces S.baicalensis Georgi and its traditional Chinese medicine GAP base,ecologically suitable planting county and medicinal value in poor areas,and studies the intellectual property resources related to S.baicalensis Georgi,such as traditional knowledge,biological genetic resources,patents,new plant varieties,geographical indications,and trademarks.This paper mainly discusses the inheritance essence,innovation and high-quality development strategy of S.baicalensis Georgi under the rural revitalization strategy and traditional Chinese medicine development strategy from the aspects of intellectual property rights,important agricultural cultural heritage,advantageous areas of characteristic agricultural products and so on.

The Significant Role of the inheritance of Traditional Chinese Culture in Sports Development

The development of Chinese sports undertakings has inherited the connotation of traditional Chinese culture,effectively interpreting the national spirit,temperament and courage. Hence,while constructing Chinese sports culture and sports humanistic ideas,we can abide by the ideas of the traditional culture,thereby driving C hinese sports towards the innovative direction of national sport,ecological sports,leisure sports. On this basis,it is necessary to emphasize newrequirements for the dynamic development of sports spirit,sports humanity and sports culture,to fully display the intrinsic value of traditional culture in sports undertakings,which is conducive to accelerating the newnormal of sports undertakings in C hina.

Inheritance and Sustainable Development of Concession Gardens of Tianjin City,China

Through reviewing development history of concession gardens in Tianjin City,features,advantages and problems of these gardens were analyzed.It was stressed that cultural context should be inherited,and features of the city highlighted.The gardens should be protected from the perspective of development,combined with urban green space system,integrated with cultural life of local citizens,and coordinated with contemporary architectural style of the city.In addition,plant landscapes should be enriched,and infrastructure of the gardens supplemented to properly handle the relationship between urban construction and protection of historical image,and provide reference for inheriting cultural context of the city in the urban development.

Inheritance and Sustainable Development: Countermeasures for the Development of Tianjin Concession Parks

Through the overview of the development history of Tianjin Concession Parks, this paper analyzed the historical significance of Tianjin Concession Parks and put forward its existing problems, then summed up a series of strategies of sustainable development as follows: adhering to the Florence Charter; retaining the original form and texture of the Concession Parks; adopting a dynamic philosophy of protection and preservation; integrating with citizens' social life; supplementing and improving infrastructure facilities; and achieving environmental regeneration by planting. All these efforts are expected to contribute to making a balance between the development and construction of urban cities and the preservation of historical features, as well as provide lessons for inheriting urban history and culture in the future.

Strengthen the Four Confidences:Inheritance and Development of the Spirit of the May 4th Movement

The spirit of the May 4th Movement has a profound influence on China’s self-confidence in its path,political system,guiding theories,and culture.This spirit,which conceived the choice of socialist path,has become the foundation of confidence in the socialist path with Chinese characteristics.The Marxist theory contained in the spirit is the core of the theoretical self-confidence of socialism;the socialist system bred by the spirit has borne the fruit of confidence in the socialist system with Chinese characteristics;the new culture movement produced by the spirit is the thrust of cultural self-confidence of socialism with Chinese characteristics.Inheriting and developing the spirit of the May Fourth Movement in the new era is conducive to strengthen the Four Confidences.

Development of organelle single nucleotide polymorphism (SNP) markers and their application for the identification of cytoplasmic inheritance patterns in Pyropia yezoensis (Bangiales,Rhodophyta)

The genus Pyropia contains several important cultivated species.Genetic research in nori species has mainly focused on the cell nucleus,with few studies on organelles(chloroplast and mitochondria).Due to the high copy numbers of organelles in cells,which influence the development and traits of algae,it is necessary to study their genetic mechanism.In this study,the marine red alga Pyropia yezoensis,an important economic macroalga,was selected as the study object.To investigate organelle(chloroplast and mitochondria)inheritance in P.yezoensis,the wild type RZ(maternal strain)was crossed with the red mutant HT(paternal strain)and 30 color-sectors from 11 F1 gametophytic blades were examined.The complete chloroplast and mitochondrial genomes of the red mutant(HT)were assembled for the first time.One reliable and stable single nucleotide polymorphism(SNP)loci filtrated by bioinformatics analysis was used as a molecular marker for chloroplast and mitochondrial DNA,respectively,in subsequent experiments.PCR amplification and sequence analysis showed that the haplotypes of color-sectors detected were consistent with those of the maternal parent,confirming that both chloroplast and mitochondrial genomes were inherited maternally in P.yezoensis.The inheritance pattern of organelles in P.yezoensis can be used to guide the hybridization and breeding of nori.Additionally,the organelle SNP markers developed in this study can be applied in subsequent genetic research.

Inheritance,Innovation,Development and Intellectual Property Protection of Tea Industry in Wuling Mountain Area of Hubei Province

Wuling Mountain Area of Hubei Province is a key tea-producing area in China,and the tea industry is its agricultural pillar industry.First,this paper introduces the tea industry in Wuling Mountain Area.Then,from traditional knowledge,biological genetic resources,new plant varieties,patents,geographical indications,trademarks,etc.,it studies the intellectual property resources of its tea industry.Next,it analyzes main problems of its intellectual property protection and inheritance,innovation and development.It also discusses the strategy of inheritance,innovation and high-quality development of the tea industry in this ethnic area in the context of the strategy of strengthening the country with intellectual property rights and the strategy of rural revitalization.Finally,it comes up with pertinent recommendations,including implementing the traditional tea production process and technical protection project,protecting the biological genetic resources of tea trees,cultivating new varieties of tea plants bred independently,creating high value patents,building regional public brands,and strengthening intellectual property protection.

Inheritance and Development of Guangxi Minority Musical Instruments and Their Integration With Operas

The traditional musical instrument of minorities converges the evolution of ethnic group culture and history,and ethnic musical instruments are a core component of minority culture.The traditional instruments of Guangxi minorities are rich and varied,and an all-round cultural inheritance and protection mechanism should be used for ethnic musical instruments,so that the performance form of classical musical instruments of minorities can be more widely understood and mastered.Therefore,in this paper,we discuss the specific practice idea of the inheritance and development of classical musical instruments of Guangxi minorities,and facilitate the innovation of development path of ethnic musical instruments by taking the inheritance form of integrating musical instruments with operas.

Characteristics and Commonness:Regional Cultural Development and Inheritance of Chinese Excellent Culture

Different regional cultures in modern China have their own characteristics while sharing some commonness,due to regional traits as well as common routes for development.Shanxi merchants’culture,as a typical representative of Chinese regional culture,reflects Chinese spirits,such as hard work,innovation and honesty and so on from the history of its development,change and cultural connotation,which reveals its macro commonness and micro characteristics.The essence of Shanxi merchants’culture can promote China’s economic development as the excellence of Chinese culture.

The Research on Historical Evolution and Inheritance Development of Ansai waist drum in China

This article from the perspective of sports science and sociology to explain the origin and development ofansai waist drum, analyzes the historical evolution of ansai waist drum, analysis of ansai waist drum in problems existing in the process of development, and according to the analysis and the reality put forward the countermeasures of development of ansai waist drum inheritance： transmission of ansai waist drum based on school, and innovation of art form, ＂waist drum troupe＂ building, the establishment of various rules and regulations and the drummer cultivation mechanism and strengthening network, books, magazines and other publicity and performances.

On the Inheritance and Development of Local Traditional Food-Take Quzhou Longyou Sponge Cake as an Example

Chinese traditional food always refers to the food with complete color,fragrance,and taste,each of which has its own unique cultural connotation and embodies China’s extensive and profound traditional culture.As one of the traditional specialties in Quzhou,Longyou Sponge Cake has a unique regional flavor and cultural heritage.The written history of Sponge Cake originated in the Ming Dynasty.In the 600-year long history,Sponge Cake has a deep historical and cultural connotation,which is deeply loved by the local people and gradually forms a unique cake culture.However,in recent years,due to insufficient publicity,cumbersome production techniques,lack of“culture+”brand strategy development,and other issues,it is difficult to be promoted and inherited by the local government and people,and it is difficult to go out of Quzhou and into the vision of Chinese people.Therefore,it is extremely important to study and explore a path suitable for the sustainable development of Sponge Cake in view of the development status of Quzhou’s characteristic food Sponge Cake.