In aquaculture,it is important to estimate in advance how much food cultured animals would take.The rate of food consumption by cultured animals to available food amount is defined as the food intake rate(FIR) in this...In aquaculture,it is important to estimate in advance how much food cultured animals would take.The rate of food consumption by cultured animals to available food amount is defined as the food intake rate(FIR) in this paper.To some extents,FIR reflects the quality of food,the health of cultured animals and the delivery efficiency.In practice,it is difficult to estimate in advance the accurate quantity of food that cultured animal needs.Usually,food is provided more than the need by animals,causing excess food that may pollute water and environment.Our experiments in past years show that FIR at 80% is recommended.展开更多
The objective of this study was to examine the effects of addition of inoculant to high-nitrogen (N) fertilized timothy on fermentation, nutritive value, and feed intake of silage. The silage of timothy cultivated wit...The objective of this study was to examine the effects of addition of inoculant to high-nitrogen (N) fertilized timothy on fermentation, nutritive value, and feed intake of silage. The silage of timothy cultivated with a N fertilizer rate at two levels (high level (H), standard level (S)) was prepared with (SI, HI) or without (SC, HC) an inoculants. The CP content of H increased by 38 g·kg-1 DM compared with that of S, and the WSC and ADF contents decreased compared with those of S. Regarding the fermentation of silage, the pH and NH3-N ratio was significantly lower in the silage with inoculant (SI and HI), showing improvement of the fermentation compared with those of the silage without the addition (SC and HC). The CP content was significantly higher in HC and HI than in SC and SI, and the NDF content was the lowest in HI among the four treatments. The CP digestibility of HC and HI was significantly higher than those of SC and SI. The EE digestibility of SI was significantly higher than that of SC, and that of HI was significantly higher than that of HC. The DCP contents were significantly higher in HC and HI than in SC and SI. The TDN content was the lowest in SC and highest in HI. The DCP intakes of HC and HI were significantly higher than those of SC and SI. When the N fertilizer rate was increased, the DCP content and DCP intake of the silage increased, and the addition of inoculant improved the fermentation and increased the TDN content.展开更多
Reproduction is the highest energy demand period for small mammals, during which both energy intake and expenditure are increased to cope with elevated energy requirements of offspring growth and somatic protection. O...Reproduction is the highest energy demand period for small mammals, during which both energy intake and expenditure are increased to cope with elevated energy requirements of offspring growth and somatic protection. Oxidative stress life history theory proposed that reactive oxygen species（ROS） were produced in direct proportion to metabolic rate, resulting in oxidative stress and damage to macromolecules. In the present study, several markers of oxidative stress and antioxidants activities were examined in brain, liver, kidneys, skeletal muscle and small intestine in non-lactating（Non-Lac） and lactating（Lac） KM mice. Uncoupling protein（ucps） gene expression was examined in brain, liver and muscle. During peak lactation, gross energy intake was 254% higher in Lac mice than in Non-Lac mice. Levels of H2O2 of Lac mice were 17.7% higher in brain（P〈0.05）, but 21.1%（P〈0.01） and 14.5%（P〈0.05） lower in liver and small intestine than that of Non-Lac mice. Malonadialdehyde（MDA） levels of Lac mice were significantly higher in brain, but lower in liver, kidneys, muscle and small intestine than that of Non-Lac mice. Activity of glutathione peroxidase（GSH-PX） was significantly decreased in brain and liver in the Lac group compared with that in the Non-Lac group. Total antioxidant capacity（TAOC） activity of Lac mice was significantly higher in muscle, but lower in kidneys than Non-Lac mice. Ucp4 and ucp5 gene expression of brain was 394% and 577% higher in Lac mice than in Non-Lac mice. These findings suggest that KM mice show tissuedependent changes in both oxidative stress and antioxidants. Activities of antioxidants may be regulated physiologically in response to the elevated ROS production in several tissues during peak lactation. Regulations of brain ucp4 and ucp5 gene expression may be involved in the prevention of oxidative damage to the tissue.展开更多
Entomophagy has gained popularity as a novel approach to addressing food and nutrition insecurity, particularly in sub-tropical and tropical countries. Cockroaches, for example, have the potential to alleviate nutriti...Entomophagy has gained popularity as a novel approach to addressing food and nutrition insecurity, particularly in sub-tropical and tropical countries. Cockroaches, for example, have the potential to alleviate nutritional deficiencies, as well as the erratic supply of protein in feeds. They can also be reared on locally available organic substrates. This study investigated growth performance and survival of german cockroach (Blattella germanica) reared on locally available organic matter substrates as treatments;spent brewers’ grain (Treatment A), Treatment B consisting of (40%: wheat bran: 40% spent brewers’ grain: 20% Caridina nilotica), wheat bran (Treatment C), and C. nilotica (Treatment D) and in a completely randomised design (CRD). Each treatment was replicated four times, while in each replicate 20 - 30 nymphs were reared for forty-two days. Feed intake for all the treatments was not significantly different. There was a significant difference (P 0.05) in mature weight, average daily weight gain, live weight, and survival during rearing period. Cockroach performed well on treatment B and recorded highest mature weight, overall performance index, survival was 90.25 mg, 197.35, 96%. The least perfomance was reported in Treatment C;1.78, 83, 143.17 and 72.5 for average growth rate, survival, overall perfomance and mature weight respectively. B. Based on these findings, a single feed as used in this study was inferior to the composite (Treatment B) in all parameters of growth performance and survival. The composite diet (Treatment B) could be suitable substrate for mass production of B. germanica for feed and food.展开更多
Deprivation is an environmental experience that organisms are continually exposed to. However, few studies analyze deprivation effects on the consumption pattern during the post-deprivation period systematically. The ...Deprivation is an environmental experience that organisms are continually exposed to. However, few studies analyze deprivation effects on the consumption pattern during the post-deprivation period systematically. The aim of this study was to evaluate the deprivation effect on feeding pattern, growth rate and body weight during post-deprivation. Sixteen albino rats (3 months old at the beginning of the experiment) were exposed to 72-hour total food deprivation every 15 days. Food and water were freely available during non-deprivation periods. Rats completed three cycles comprised by a free access period followed by food deprivation. Once deprivation was removed, food and water consumption increased and then decreased, while body weight was recovered. Growth rate increased after every deprivation period and reached similar levels to those presented by the control group. Depriving rats from one commodity (i.e., food) cause them to restrain their consumption of the other freely available commodity (i.e., water). Results confirm that food deprivation modifies growth rate, water and food consumption. Additionally, during post-deprivation period, differences between males and females were not registered.展开更多
Purpose: Heart rate variability (HRV) is acknowledged as a useful tool to estimate autonomic function. Fast Fourier transform (FFT) and autoregressive model (AR) are used for power spectral analysis of HRV. However, t...Purpose: Heart rate variability (HRV) is acknowledged as a useful tool to estimate autonomic function. Fast Fourier transform (FFT) and autoregressive model (AR) are used for power spectral analysis of HRV. However, there is little evidence of agreement between FFT and AR in relation to HRV following food intake in females. In the present study, we applied both FFT and AR after food intake during the follicular and luteal phases, and compared raw low-frequency (LF) and high- frequency (HF) powers, and LF/HF ratio obtained with the two power-spectral analytical methods. Methods: All subjects participated in two sessions: follicular phase session and luteal phase session. In each session, R-R intervals were continuously recorded before and after meals, and power spectral analysis of heart rate variability was performed. We analyzed low-frequency power (LF: 0.04 - 0.15 Hz) and high-frequency power (HF: 0.15 - 0.40 Hz) by using FFT and AR. LF and HF power were computed for each 30 sec, 1 min, 2.5 min, and 5 min of the 5-min R-R data before meal intake and at 20, 40, 60 and 80 min after meal intake. The LF/HF ratio was calculated as an index of sympathovagal balance. Results: In the present study, after 30 sec and 1 min of segment analysis, there was little interchangeability between AR and FFT in LF, HF, and LF/HF ratio in both follicular and luteal phases. In 2.5 min or 5 min of segment analysis, there was interchangeability between FFT and AR in LF and HF, but not in the LF/HF ratio in both follicular and luteal phases. Additionally, FFT underestimated HRV compared with AR, and the extent of underestimation increased with increasing AR value. Conclusion: FFT underestimated HRV compared with AR, and FFT correlated poorly with AR when the analysis segment was shortened.展开更多
基金Supported by the National Science Foundation of China, Key Lab. of Marine Ecology & Environment, Ministry of Education, Ocean University of Chinathe National High-Technology R&D Program of China (863 Program, No. 2006AA09Z418)
文摘In aquaculture,it is important to estimate in advance how much food cultured animals would take.The rate of food consumption by cultured animals to available food amount is defined as the food intake rate(FIR) in this paper.To some extents,FIR reflects the quality of food,the health of cultured animals and the delivery efficiency.In practice,it is difficult to estimate in advance the accurate quantity of food that cultured animal needs.Usually,food is provided more than the need by animals,causing excess food that may pollute water and environment.Our experiments in past years show that FIR at 80% is recommended.
文摘The objective of this study was to examine the effects of addition of inoculant to high-nitrogen (N) fertilized timothy on fermentation, nutritive value, and feed intake of silage. The silage of timothy cultivated with a N fertilizer rate at two levels (high level (H), standard level (S)) was prepared with (SI, HI) or without (SC, HC) an inoculants. The CP content of H increased by 38 g·kg-1 DM compared with that of S, and the WSC and ADF contents decreased compared with those of S. Regarding the fermentation of silage, the pH and NH3-N ratio was significantly lower in the silage with inoculant (SI and HI), showing improvement of the fermentation compared with those of the silage without the addition (SC and HC). The CP content was significantly higher in HC and HI than in SC and SI, and the NDF content was the lowest in HI among the four treatments. The CP digestibility of HC and HI was significantly higher than those of SC and SI. The EE digestibility of SI was significantly higher than that of SC, and that of HI was significantly higher than that of HC. The DCP contents were significantly higher in HC and HI than in SC and SI. The TDN content was the lowest in SC and highest in HI. The DCP intakes of HC and HI were significantly higher than those of SC and SI. When the N fertilizer rate was increased, the DCP content and DCP intake of the silage increased, and the addition of inoculant improved the fermentation and increased the TDN content.
基金supported by grants from Wenzhou University（14SK51A,14SK52A）the National Natural Science Foundation of China（31270458）grant from Zhejiang Province（pd2013374）
文摘Reproduction is the highest energy demand period for small mammals, during which both energy intake and expenditure are increased to cope with elevated energy requirements of offspring growth and somatic protection. Oxidative stress life history theory proposed that reactive oxygen species（ROS） were produced in direct proportion to metabolic rate, resulting in oxidative stress and damage to macromolecules. In the present study, several markers of oxidative stress and antioxidants activities were examined in brain, liver, kidneys, skeletal muscle and small intestine in non-lactating（Non-Lac） and lactating（Lac） KM mice. Uncoupling protein（ucps） gene expression was examined in brain, liver and muscle. During peak lactation, gross energy intake was 254% higher in Lac mice than in Non-Lac mice. Levels of H2O2 of Lac mice were 17.7% higher in brain（P〈0.05）, but 21.1%（P〈0.01） and 14.5%（P〈0.05） lower in liver and small intestine than that of Non-Lac mice. Malonadialdehyde（MDA） levels of Lac mice were significantly higher in brain, but lower in liver, kidneys, muscle and small intestine than that of Non-Lac mice. Activity of glutathione peroxidase（GSH-PX） was significantly decreased in brain and liver in the Lac group compared with that in the Non-Lac group. Total antioxidant capacity（TAOC） activity of Lac mice was significantly higher in muscle, but lower in kidneys than Non-Lac mice. Ucp4 and ucp5 gene expression of brain was 394% and 577% higher in Lac mice than in Non-Lac mice. These findings suggest that KM mice show tissuedependent changes in both oxidative stress and antioxidants. Activities of antioxidants may be regulated physiologically in response to the elevated ROS production in several tissues during peak lactation. Regulations of brain ucp4 and ucp5 gene expression may be involved in the prevention of oxidative damage to the tissue.
文摘Entomophagy has gained popularity as a novel approach to addressing food and nutrition insecurity, particularly in sub-tropical and tropical countries. Cockroaches, for example, have the potential to alleviate nutritional deficiencies, as well as the erratic supply of protein in feeds. They can also be reared on locally available organic substrates. This study investigated growth performance and survival of german cockroach (Blattella germanica) reared on locally available organic matter substrates as treatments;spent brewers’ grain (Treatment A), Treatment B consisting of (40%: wheat bran: 40% spent brewers’ grain: 20% Caridina nilotica), wheat bran (Treatment C), and C. nilotica (Treatment D) and in a completely randomised design (CRD). Each treatment was replicated four times, while in each replicate 20 - 30 nymphs were reared for forty-two days. Feed intake for all the treatments was not significantly different. There was a significant difference (P 0.05) in mature weight, average daily weight gain, live weight, and survival during rearing period. Cockroach performed well on treatment B and recorded highest mature weight, overall performance index, survival was 90.25 mg, 197.35, 96%. The least perfomance was reported in Treatment C;1.78, 83, 143.17 and 72.5 for average growth rate, survival, overall perfomance and mature weight respectively. B. Based on these findings, a single feed as used in this study was inferior to the composite (Treatment B) in all parameters of growth performance and survival. The composite diet (Treatment B) could be suitable substrate for mass production of B. germanica for feed and food.
文摘Deprivation is an environmental experience that organisms are continually exposed to. However, few studies analyze deprivation effects on the consumption pattern during the post-deprivation period systematically. The aim of this study was to evaluate the deprivation effect on feeding pattern, growth rate and body weight during post-deprivation. Sixteen albino rats (3 months old at the beginning of the experiment) were exposed to 72-hour total food deprivation every 15 days. Food and water were freely available during non-deprivation periods. Rats completed three cycles comprised by a free access period followed by food deprivation. Once deprivation was removed, food and water consumption increased and then decreased, while body weight was recovered. Growth rate increased after every deprivation period and reached similar levels to those presented by the control group. Depriving rats from one commodity (i.e., food) cause them to restrain their consumption of the other freely available commodity (i.e., water). Results confirm that food deprivation modifies growth rate, water and food consumption. Additionally, during post-deprivation period, differences between males and females were not registered.
文摘Purpose: Heart rate variability (HRV) is acknowledged as a useful tool to estimate autonomic function. Fast Fourier transform (FFT) and autoregressive model (AR) are used for power spectral analysis of HRV. However, there is little evidence of agreement between FFT and AR in relation to HRV following food intake in females. In the present study, we applied both FFT and AR after food intake during the follicular and luteal phases, and compared raw low-frequency (LF) and high- frequency (HF) powers, and LF/HF ratio obtained with the two power-spectral analytical methods. Methods: All subjects participated in two sessions: follicular phase session and luteal phase session. In each session, R-R intervals were continuously recorded before and after meals, and power spectral analysis of heart rate variability was performed. We analyzed low-frequency power (LF: 0.04 - 0.15 Hz) and high-frequency power (HF: 0.15 - 0.40 Hz) by using FFT and AR. LF and HF power were computed for each 30 sec, 1 min, 2.5 min, and 5 min of the 5-min R-R data before meal intake and at 20, 40, 60 and 80 min after meal intake. The LF/HF ratio was calculated as an index of sympathovagal balance. Results: In the present study, after 30 sec and 1 min of segment analysis, there was little interchangeability between AR and FFT in LF, HF, and LF/HF ratio in both follicular and luteal phases. In 2.5 min or 5 min of segment analysis, there was interchangeability between FFT and AR in LF and HF, but not in the LF/HF ratio in both follicular and luteal phases. Additionally, FFT underestimated HRV compared with AR, and the extent of underestimation increased with increasing AR value. Conclusion: FFT underestimated HRV compared with AR, and FFT correlated poorly with AR when the analysis segment was shortened.
