Bi-doped carbon quantum dots functionalized liposomes with fluorescence visualization imaging for tumor diagnosis and treatment

Liposomes are one of the significant classes of antitumor nanomaterials and the most successful nanomedicine drugs in clinical translation. However, it is difficult to accurately reveal liposome delivery modes and drug release rates at different p H values to assess the biodistribution and drug delivery pathways in vivo. Here, we established a strategy to integrate Bi-doped carbon quantum dots(CQDs)with liposomes to produce fluorescence visualization and therapeutic effects, namely lipo/Bi-doped CQDs.Lipo/Bi-doped CQDs show good water solubility and physicochemical properties, which can be used for in vitro labeling of colon cancer(CT26) cells and in vivo imaging localization tracking tumors for monitoring. Simultaneously, thanks to the excellent p H sensitivity and ion doping characteristic of Bi-doped CQDs, lipo/Bi-doped CQDs can be used to reveal the drug release rate of liposomes at different p H values and exhibit potential effects in vivo antitumor therapy.

The Theoretical Defects in DRIS and the Restructuring of a New Approach

There are two kinds of theoretical defects export in the diagnosis and recommendationintegrated system (DRIS): the distribution of nutrient elements ratios with two normaldistribution displays an abnormal distribution of positive skewness; there is a blinddiagnostic area by DRIS on the occasion of the nutrient elements with equal ratios butwithout equal quantity. In the light of the quadratic form theory of multidimensionalnormal distribution and the view of balance of equal probability, the balance diagnosisand recommendation integrated system (BDRIS) was developed in this paper, which issuperior to DRIS and its diagnosis method is unified to critical value diagnosis. Whenthe correlation matrix of nutrient elements R=I(identity matrix), i.e., the effects ofelements antagonism is disregarded, BDRIS will be simplified into the critical valuediagnosis. In addition, the diagnosis program written in SAS language was also providedin this paper.

Preparation of NIR-II-excited Drug-loaded Liposomes and Their Antitumor Activity and in Vitro Imaging Studies

In recent years,with the rapid development of nano-technology,the combination of diagnosis and treatment by nanotechnologyhas brought new hope for mankind to overcome cancer.The Near-infrared two-region(NIR-II)imaging technology hasdeveloped rapidly in recent years because of its non-invasive nature,strong tissue penetration and overall imaging of small animals.In this study,a novel nano-drug delivery system(DOX-IR1061 cationic liposomes)was prepared,loaded with doxorubicin(DOX)as a chemotherapeutic drug and NIR-II-excited fluorescent probe IR1061 as an imaging agent,and the uptake ability of tumor cellswas enhanced by octadecylamine.DOX-IR1061 cationic liposomes have good NIR-II imaging ability,clear imaging and obvioussignal.Cell uptake indicated that palamine could enhance the uptake efficiency of cationic liposomes by tumor cells,while in vitroanti-tumor experiments revealed that the enhancement of uptake efficiency would enhance the anti-tumor effect of DOX.Therefore,DOX-IR1061 cationic liposomes we prepared have the potential to realize both tumor imaging and therapy,and to realize theintegration of rapid diagnosis and treatment,and provide new ideas for cancer cure and the development of the field of diagnosis andtreatment integration.

Thinking and Strategy on the Diagnosis and Treatment of Functional Gastrointestinal Disorders with Integrative Medicine

The functional gastrointestinal disorders (FGIDs) are a group of diseases mainly manifested as gastrointestinal functional disorders,including 45 kinds of different diseases,such as functional dyspepsia(FD), irritable bowel syndrome (IBS),functional constipation

Experiences on the Integrative Medical Diagnosis and Treatment of Acute Kidney Injury

The definition of acute renal failure（ARF） has not gotten common understanding yet in a long time,which leads to the difficulty in comparing the outcomes of some different studies,and has impacted the advance of diagnosis and treatment on the illness to certain extents. Most of the scholars hold that the attention paid to the early diagnosis and intervention of ARF was insufficient in recent years. Lots of clinical researchers indicated that even a slight impairment of renal function could result in the increasing of the morbidity and mortality of ARF.

Surface-enhanced Raman scattering technology based on TiO_(2)/Nb_(2)C coated microfluidic chip for monitoring glioma cells invasion in real time

Glioma is a malignant primary brain tumor that is extremely harmful to human beings.Therefore,studying the invasiveness of glioma cells is of great significance for the diagnosis and treatment of glioma.In this work,TiO_(2)/Nb_(2)C was prepared as a SERS substrate and combined with microfluidic chip to construct an invasion model capable of monitoring glioma invasion in real time.Both experimental data and density function theory(DFT)calculations showed that the significant SERS-enhancing effect of TiO_(2)/Nb_(2)C on methylene blue(MB)originated from the chemical magnification(CM)mechanism when MB was used as the adsorbed molecule.Based on this,we achieved a highly sensitive and targeted detection of vascular endothelial growth factor(VEGF),a biomarker for glioma with a low detection limit of 3.7 pg/m L,then quantified the invasive process in real time by detecting VEGF.Meanwhile,the depletion of reactive oxygen species(ROS)by TiO_(2)/Nb_(2)C can inhibit the invasion of glioma cells.For the first time,the invasion model combines SERS technology with microfluidic technology,while monitoring the cell invasion process in real time,the invasion process can be quantified by detecting the VEGF secreted by glioma cells during the invasion process,realizing the integration of diagnosis and treatment,and establish a new model for the biomedical analysis,clinical diagnosis and treatment of glioma.