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Global Climate Internal Variability in a 2000-year Control Simulation with Community Earth System Model(CESM) 被引量:13
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作者 WANG Zhiyuan LI Yao +1 位作者 LIU Bin LIU Jian 《Chinese Geographical Science》 SCIE CSCD 2015年第3期263-273,共11页
Using the low-resolution (T31, equivalent to 3.75°× 3.75°) version of the Community Earth System Model (CESM) from the National Center for Atmospheric Research (NCAR), a global climate simulation ... Using the low-resolution (T31, equivalent to 3.75°× 3.75°) version of the Community Earth System Model (CESM) from the National Center for Atmospheric Research (NCAR), a global climate simulation was carried out with fixed external forcing factors (1850 Common Era. (C.E.) conditions) for the past 2000 years. Based on the simulated results, spatio-temporal structures of surface air temperature, precipitation and internal variability, such as the E1 Nifio-Southem Oscillation (ENSO), the Atlantic Multi-decadal Oscilla- tion (AMO), the Pacific Decadal Oscillation (PDO), and the North Atlantic Oscillation (NAO), were compared with reanalysis datasets to evaluate the model performance. The results are as follows: 1) CESM showed a good performance in the long-term simulation and no significant climate drift over the past 2000 years; 2) climatological patterns of global and regional climate changes simulated by the CESM were reasonable compared with the reanalysis datasets; and 3) the CESM simulated internal natural variability of the climate system performs very well. The model not only reproduced the periodicity of ENSO, AMO and PDO events but also the 3-8 years vari- ability of the ENSO. The spatial distribution of the CESM-simulated NAO was also similar to the observed. However, because of weaker total irradiation and greenhouse gas concentration forcing in the simulation than the present, the model performances had some differences from the observations. Generally, the CESM showed a good performance in simulating the global climate and internal natu- ral variability of the climate system. This paves the way for other forced climate simulations for the past 2000 years by using the CESM. 展开更多
关键词 Community Earth System Model (CESM) climate simulation past 2000 years climate system intemal variability
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In-situ measure to internal stress of shotcrete layer in soft-rock roadway 被引量:13
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作者 Shulin Fang Jian Zhang 《International Journal of Coal Science & Technology》 EI CAS 2014年第3期321-328,共8页
In order to solve the difficult conditions of soft rock,water-trickling and hard-maintain of main air-return roadway in Tarangaole Colliery,high pretensioned stress and intensive bolt-shotcrete support program was des... In order to solve the difficult conditions of soft rock,water-trickling and hard-maintain of main air-return roadway in Tarangaole Colliery,high pretensioned stress and intensive bolt-shotcrete support program was designed and mechanical property of shotcrete layer was specially monitored through utilizing a type of concrete stress meter with oscillating chord after the program was carried out.It was indicated that,due to rock pressure and support resistance,the interior of shotcrete layer would emerge diverse stresses in axial,radial and tangential directions.With time passing internal stresses in three directions,whose average values were-0.061,0.043 and 0.517 MPa respectively,fluctuated first and then tended to stability slowly.The axial and radial stresses were relatively smaller than tangential stress which was 11,12 times the two formers respectively.Along the section of roadway,axial and tangential stresses distributed symmetrically and increased gradually from the top of arch to the waist of wall,but reduced at the foot of wall.Radial stresses reduced from the top of arch to the waist of arch first,and then increased in the waist of wall.Axial stresses were tensile substantially,except for stresses in arch vault tending to compressive,but all the radial stresses were compressive.Nevertheless,tangential stresses in the wall were compressive and tangential stresses in the arch were tensile.During the period of roadway excavating,the stress of shotcrete layer was less than its ultimate bearing capacity,with no significant stress concentration.At the end of this article,some suggests are given to shotcrete support design. 展开更多
关键词 Soft-rock roadway Shotcrete layer intemal stress measure Oscillating-chord stress meter
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Application of Self-inhaling Internal Cooling Wheel in Vertical Surface Grinding 被引量:9
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作者 LI Xun 《Chinese Journal of Mechanical Engineering》 SCIE EI CAS CSCD 2014年第1期86-91,共6页
There is less research on vertical sculptured grinding technology. Especially in high vertical surface grinding process with the cup abrasive wheel, the thermal damage is prone to happen and undermine the grinding sur... There is less research on vertical sculptured grinding technology. Especially in high vertical surface grinding process with the cup abrasive wheel, the thermal damage is prone to happen and undermine the grinding surface integrity. This problem limits to improve the grinding efficiency and the grinding ratio greatly. Through the analysis of vertical surface grinding process and features in depth, this paper revealed the inherent mechanism of higher grinding temperature in the process of vertical sculptured grinding using the cup wheel. Based on the previous research achievements, the grinding experiments on TC4 (Ti-6A1-4V) and GH4169 are carried out utilizing the self-inhaling internal cooling wheel. The experimental results show that the self-inhaling internal cooling wheel can efficiently reduce the grinding surface temperature. Moreover, the inherent mechanism of reducing the grinding temperature using the internal cooling method is revealed. Meanwhile, under the same grinding conditions, the grinding ratio during the experiments on GH4169 using self-inhaling internal cooling method is about 3 times as high as using conventional external cooling method. And the grinding forces can be reduced by about 20%. This research revealed the inherent mechanism of higher grinding temperature in the process of vertical sculptured grinding using the cup wheel, which provides theoretical basis for the design and application of self-inhaling internal cooling wheel. At the same time, an efficient and non-invasive surface grinding method of TC4 and GH4169 is presented. 展开更多
关键词 vertical grinding intemal cooling grinding temperature grinding ratio
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On iterative learning control design for tracking iteration-varying trajectories with high-order internal model 被引量:7
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作者 Chenkun YIN Jianxin XU Zhongsheng HOU 《控制理论与应用(英文版)》 EI 2010年第3期309-316,共8页
In this paper, iterative learning control (ILC) design is studied for an iteration-varying tracking problem in which reference trajectories are generated by high-order internal models (HOLM). An HOlM formulated as... In this paper, iterative learning control (ILC) design is studied for an iteration-varying tracking problem in which reference trajectories are generated by high-order internal models (HOLM). An HOlM formulated as a polynomial operator between consecutive iterations describes the changes of desired trajectories in the iteration domain and makes the iterative learning problem become iteration varying. The classical ILC for tracking iteration-invariant reference trajectories, on the other hand, is a special case of HOlM where the polynomial renders to a unity coefficient or a special first-order internal model. By inserting the HOlM into P-type ILC, the tracking performance along the iteration axis is investigated for a class of continuous-time nonlinear systems. Time-weighted norm method is utilized to guarantee validity of proposed algorithm in a sense of data-driven control. 展开更多
关键词 ILC High-order intemal model Iteration-varying Nonlinear systems CONTINUOUS-TIME
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SIMULTANEOUS ANTERIOR AND POSTERIOR HEMIVERTEBRA RESECTION IN THE TREATMENT OF CONGENITAL KYPHOSCOLIOSIS 被引量:2
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作者 Li-xiang Ding Gui-xing Qiu Yi-peng Wang Jian-guo Zhang 《Chinese Medical Sciences Journal》 CAS CSCD 2005年第4期252-256, ,共5页
Objective To evaluate the efficacy of simultaneous anterior and posterior hemivertebra resection in the treatment of congenital kyphoscoliosis caused by fully-segmented hemivertebra. Methods Twenty-one consecutive ca... Objective To evaluate the efficacy of simultaneous anterior and posterior hemivertebra resection in the treatment of congenital kyphoscoliosis caused by fully-segmented hemivertebra. Methods Twenty-one consecutive cases with congenital kyphoscoliosis, which were 8 males and 13 females, underwent one-stage operation of anterior hemivertebra resection and posterior instrumentation. All of the hemivertebra were fully-segmented. The average age at surgery was 11.4 (range, 1.5-16) years old. Results The average follow-up was 23 (range, 6-50) months. The average Cobb's angle of the coronal curve was 51.4° (30°-120°) before surgery, 16.9° (0°-54°) after surgery, and 19.5° at latest follow-up. The angle of segmental kyphosis was 37.1° (0°-95°) before surgery and 16.1° (0°-48°) after surgery, and 18.2° at latest follow-up. Apical translation was improved from 4.7 cm to 1.9 cm. The average fusion segments were 4.8 (range, 2-10) segments. Complication included pressure sore in one case, pedicle cutting by pedicle screw in one case and elongation of the curve in one case. There was no obvious pseudarthrosis, decompensation, and kyohosis deformity aggravation at latest follow-up. Conclusion One-stage hemivortebra resection has a good result in the surgical treatment of congenital kyphoscoliosis caused by fully-segmented hemivertebra, and may shorten fusion levels if performed at an earlier age. 展开更多
关键词 congenital scoliosis KYPHOSIS hemivertebra resection intemal fixators
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Backlash analysis of a new planetary gearing with internal gear ring 被引量:2
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作者 朱才朝 肖宁 《Journal of Chongqing University》 CAS 2010年第3期151-158,共8页
Due to low carrying capacity and short life of current ring reducer,we proposed a new planetary gearing with internal gear ring,which consisted of two stages.One was an involute planetary drive and the other was an N-... Due to low carrying capacity and short life of current ring reducer,we proposed a new planetary gearing with internal gear ring,which consisted of two stages.One was an involute planetary drive and the other was an N-type planetary drive with small teeth difference.The kinematic calculation was conducted based on the analysis of structural composition and working principle.Formulas of backlash calculation for the new planetary gearing were derived by using probabilistic theory and the analytical model was created at the same time.Then,main factors that affect the systematic backlash were introduced and the effects of manufacture error,misaligments and roller bearing parameters on the distribution of backlash were presented.The influence of gear backlash on systematic return difference was performed based on the backlash mathematic model proposed.The results show that the backlash is a little large,of which the backlash of bearing takes a greater part than two gearing stages.So we presented some practical methods to reduce the systematic backlash. 展开更多
关键词 planetary gearing with intemal gear ring probabilistic theory return difference gear backlash
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Cooling of a Diesel Reformate Fuelled Solid Oxide Fuel Cell by Internal Reforming of Methane: A Modelling Study 被引量:1
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作者 黄肖微 Alexander Kromp 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2013年第3期324-331,共8页
In this paper a system combining a diesel reformer using catalytic partial oxidation (CPOX) with the Solid Oxide Fuel Cell (SOFC) for Auxiliary Power Unit (APU) applications is modeled with respect to the coolin... In this paper a system combining a diesel reformer using catalytic partial oxidation (CPOX) with the Solid Oxide Fuel Cell (SOFC) for Auxiliary Power Unit (APU) applications is modeled with respect to the cooling effect provided by internal reforming of methane in anode gas channel. A model mixture consisting of 80% n-hexadecane and 20%..!-methylnaphthalin is used to simulate the commercial diesel. The modelling consists of several steps. First, equilibrium gas composition at the exit of CPOX reformer is modelled in terms oxygen to car- bon (O/C) ratio, fuel utilization ratio and anode gas recirculation. Second, product composition, especially methane content, is determined for the me.th.an, ation process at the operating temperatures ra:ng!ng from 500 ℃to 520 ℃.Finally, the cooling power provided by internal reforming of methane in SOFC fuel channel is calculated for two concepts to increase the methane content of the diesel reformate. The results show that the first concept, operating the diesel reformer at low O/C ratio and/or, recirculation rat!o, is not realizable due to high probability of coke formation, whereas the second concept, combining a methanation process with CPOX, can provide a significant cool- ing effect in addition to the conventional c?oling concept which needs higher levels of excess air. 展开更多
关键词 diesel reformate METHANATION intemal reforming COOLING model solid oxide fuel cell-auxiliary power unit
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The impact of macular surgery in different grades of epiretinal membrane 被引量:1
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作者 Cosar Batman Mehmet Citirik 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2017年第12期1877-1882,共6页
AIM:To assess the impact of macular surgery on the functional and anatomic outcomes of the patients in different grades of epiretinal membrane(ERM).METHODS:Seventy-one eyes of 71 patients who underwent 23-gauge tr... AIM:To assess the impact of macular surgery on the functional and anatomic outcomes of the patients in different grades of epiretinal membrane(ERM).METHODS:Seventy-one eyes of 71 patients who underwent 23-gauge transconjunctival sutureless pars plana vitrectomy for primary isolated ERM were evaluated in this study.RESULTS:There were 38 females(53.5%) and 33 males(46.5%).The average age of the patients was 68.1 y(range 42-89 y).Mean follow up period was 14 mo(range 6-26 mo).The cases were divided into two subgroups of cellophane maculopathy(CM) and macular pucker(MP).An improvement was observed in the postoperative best-corrected visual acuity(BCVA),as well as a decrement in central foveal thickness(CFT) in both groups(both of these being statistically significant;P=0.001).In comparison between two groups,it was found that there was a significant improvement on BCVA and CFT in CM group than MP group(P=0.01).Furthermore,the postoperative fundus findings regarding RPE alterations and macular edema were significantly higher in MP group when compared to the CM group(P=0.01).CONCLUSION:ERM and internal limiting membrane peeling surgery can lead to a significant reduction of CFT and visual improvements in idiopathic ERM.A long-term ERM persistence will cause unrecoverable retinal damage and visual loss. 展开更多
关键词 cellophane maculopathy epiretinal membrane intemal limiting membrane peeling macular pucker membranepeeling VITRECTOMY
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Dynamic analysis of subway station subjected to internal blast loading 被引量:1
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作者 何伟 陈健云 郭靖 《Journal of Central South University》 SCIE EI CAS 2011年第3期917-924,共8页
The study was motivated by the fact that explosion inside the subway structure may not only cause direct life loss,but also damage the subway structure and lead to further loss of lives and properties.The propagation ... The study was motivated by the fact that explosion inside the subway structure may not only cause direct life loss,but also damage the subway structure and lead to further loss of lives and properties.The propagation law of explosion wave in the subway station was analyzed and a simplified model of overpressure in the subway station was also proposed.Whereafter,the improved dynamic cam-clay model of soil and the concrete damaged plasticity constitutive model were used for the dynamic analysis of the subway station.Meanwhile,the influences of soil stiffness and burial depth on the dynamic response of the subway station were looked into.The results show that the multi-peak overpressure in the subway station does not appear,and large stresses concentrate on the central column and the floor slab of the subway station,so some special reinforcement measures should be taken in these parts.The effect of soil stiffness and burial depth on the stress of the central column is little;however,the effect on the stress of the station side wall is relatively obvious. 展开更多
关键词 subway station intemal explosion nonlinear dynamic analysis influence factor
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VIDEO-ASSISTED THORACOSCOPIC CORRECTION AND FUSION OF SCOLIOSIS 被引量:1
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作者 Bin Yu Jian-guo Zhang Gui-xing Qiu Yi-peng Wang Xin-yu Yang 《Chinese Medical Sciences Journal》 CAS CSCD 2007年第3期144-151,共8页
Objective To evaluate the operative technique and preliminary results of video-assisted thoracoscopic anterior correction and fusion of scoliosis. Methods Eleven cases underwent thoracoscopic anterior correction and f... Objective To evaluate the operative technique and preliminary results of video-assisted thoracoscopic anterior correction and fusion of scoliosis. Methods Eleven cases underwent thoracoscopic anterior correction and fusion of scoliosis from March 2003 to April 2005 in our hospital were reviewed. They were all females with an average age of 13.1 years old. Of which, 9 cases were idiopathic scoliosis, 1 case was congenital scoliosis, and 1 case was Marian syndrome scoliosis. The coronal Cobb angle and apical vertebral translation before and after surgery as well as at final follow-up were measured. The operation time, blood loss during operation, and peri-operative complications were recorded. The mean operation time was 6. 4 hours, mean instrumented vertebrae were 6.4 segments, and mean blood loss during operation was 364 mU The coronal Cobb angles of the thoracic curve before and after surgery were 45.5° and 15.4° respectively, with an average correction rate of 65.4%. The lumbar curve was corrected from 28.4° to 11.8° , with an average simultaneous correction rate of 57.2%. All of the patients were followed up regularly with an average time of 21.4 months. At the final foUow-up, the coronal Cobb angles of the thoracic and lumbar curves were 19.0° and 20. 1° , with a 3.6° and 8. 3° loss of correction, respectively. The apical vertebral translation was improved from 32. 3 mm to 10.5 mm for the thoracic curve, and from 13.1 mm to 8.2 mm for the lumbar curve. There were 6 cases with peri-opemtive complications, including 1 case of thoracic effusion, 1 case of chylothomx, 1 case of locking plug loosing, 2 cases of aggravation of the unfused lumbar curve ( 1 case also with thoracolumbar kyphosis), and 1 case with a screw tip causing a contour deformity of the aorta. And 4 of them underwent revision surgery.Conclusions Video-assisted thoracoscopic anterior correction and fusion of scoliosis has good correction capability, less intraoperative bleeding, and favorable cosmetic effect for mild and moderate thoracic scoliosis, but with higher rates of cor- rection loss of the lumbar curve and peri-operative complications. A surgeon should be cautious to perform this technique. 展开更多
关键词 SCOLIOSIS anterior fusion THORACOSCOPY intemal fixation
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Improve regression-based models for prediction of internal-bond strength of particleboard using Buckingham's pi-theorem 被引量:1
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作者 Akbar Rostampour Haftkhani Mohammad Arabi 《Journal of Forestry Research》 SCIE CAS CSCD 2013年第4期735-740,共6页
Internal bond (IB) strength is one of the most important me- chanical properties that indicate particleboard quality. The aim of this study was to find a simple regression model that considers the most important par... Internal bond (IB) strength is one of the most important me- chanical properties that indicate particleboard quality. The aim of this study was to find a simple regression model that considers the most important parameters that can influence on IB strength. In this study, IB strength was predicted by three kinds of equations (linear, quadratic, and exponential) that were based on the percentage of adhesive (8%, 9.5%, and 11%), particle size (+5, -5 +8, -8 12, and -12 mesh), and density (0.65, 0.7, and 0.75 g/cm3). Our analysis of the results (using SHAZAM 9 software) showed that the exponential function best fitted the experi- mental data and predicted the IB strength with 18~,/0 error. In order de- crease the error percentage, the Buckingham Pi theorem was used to build regression models for predicting IB strength based on particle size, 展开更多
关键词 Buckingham's Pi theorem intemal bond strength particle-board screw withdrawal resistance.
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Cerebrovascular hemodynamics in patients with cerebral arteriosclerosis
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作者 Jianbo Yang Changcong Cui Chengbin Wu 《Neural Regeneration Research》 SCIE CAS CSCD 2011年第32期2532-2536,共5页
The present study observed hemodynamic changes in 26 patients with cerebral arteriosclerosis using a cerebral circulation dynamics detector and transcranial Doppler. In patients with cerebral arteriosclerosis the bloo... The present study observed hemodynamic changes in 26 patients with cerebral arteriosclerosis using a cerebral circulation dynamics detector and transcranial Doppler. In patients with cerebral arteriosclerosis the blood supply and flow rate in the bilateral carotid arteries and the blood flow rate in the anterior cerebral and middle cerebral arteries were similar to normal controls, but the cerebral vascular resistance, critical pressure and pulsatility index were increased, and cerebral arterial elasticity and cerebral blood flow autoregulation were decreased. Compared with the lesioned hemisphere of patients with cerebral infarction, the total blood supply and blood flow rate of patients with cerebral arteriosclerosis were higher. Compared with normal subjects, patients with cerebral arteriosclerosis exhibited cognitive disturbances, mainly in short-term memory, attention, abstract capability, and spatial and executive dysfunction. Results showed that cerebral arteriosclerosis does not directly affect the blood supply of a cerebral hemisphere, but affects cognitive function. The increased cerebral vascular resistance and reduced autoregulation of cerebral blood vessels may be important hemodynamic mechanisms of arteriosclerosis-induced cerebral infarction. 展开更多
关键词 HEMODYNAMICS cerebral arteriosclerosis cognitive disturbances cerebral infarction intemal carotid
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Responses of wind-induced internal pressure in a two-compartment building with a dominant opening and background porosity Part 2:Parameter analyses and design equations
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作者 全涌 余先锋 顾明 《Journal of Central South University》 SCIE EI CAS 2012年第11期3225-3235,共11页
Analyses of the effects of some parameters were performed to determine the admittance functions in a common two-compartment building with background porosity by the imposed excitation method.Variations of the magnific... Analyses of the effects of some parameters were performed to determine the admittance functions in a common two-compartment building with background porosity by the imposed excitation method.Variations of the magnification factors of fluctuating internal pressures were analyzed using 96 model cases under random fluctuating external pressure,and then corresponding design equations were fitted.The results show that the Helmholtz resonance peaks of the admittance functions in both compartments increase with increasing the area of windward or partition wall opening.With increasing the volume of the compartment with an external opening,the resonance peak in this compartment at the higher Helmholtz frequency significantly decreases,at the same time,the resonance peak in the other compartment at the lower Helmholtz frequency also decreases.With increasing the volume of the compartment with background porosity,both resonance peaks in this compartment at the lower and higher Helmholtz frequencies decrease,meanwhile,the resonance peak at the lower Helmholtz frequency for the other compartment also decreases,whereas the resonance peak at the higher Helmholtz frequency increases.Both resonance peaks of the admittance functions in the two compartments decrease with increasing the amplitude of fluctuating external pressure coefficients or reference wind speed. 展开更多
关键词 dominant opening intemal pressure background porosity parameter analyses design equations
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Numerical Simulation on the Interaction of an Internal Solitary Wave with a Step-Type Topography
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作者 XU Zhaoting LI Qun CHEN Xu YU Jia 《Journal of Ocean University of China》 SCIE CAS 2008年第2期119-123,共5页
Based on a nonhydrostatic numerical ocean model developed by one of the authors, the interaction of an intemal solitary wave with a step-type topography was investigated. Over the step topography, the flow pattern cou... Based on a nonhydrostatic numerical ocean model developed by one of the authors, the interaction of an intemal solitary wave with a step-type topography was investigated. Over the step topography, the flow pattern could be classified into three categories: 1) the propagation and spatial structure of the internal solitary wave was little influenced by the bottom topography, 2) the internal solitary wave was significantly distorted by the blocking effect of the topography without the occurrence of wave breaking and 3) the internal solitary wave was broken as it encountered and passed over the bottom topography. A detailed description of the processes leading to wave breaking is given in this paper together with energy budget analysis. The results revealed that the maximum of the energy dissipation rate is no more than 40%, which is consistent with available experimental data. 展开更多
关键词 stratified flow intemal solitary wave energy dissipation
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Percutaneous closure of left internal mammary artery-to-pulmonary artery fistula using the domestic vascular plug:a case report
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作者 Chen Shaoping Wang Zhongkai Zhao Xianxian Qin Yongwen 《Journal of Medical Colleges of PLA(China)》 CAS 2010年第4期247-251,共5页
A 55-year-old man was admitted for transcatheter closure because of pulmonary arteriovenous fistula by magnetic resonance angiography (MRA). He had a history of occasional chest pain more than one year. Angiography ... A 55-year-old man was admitted for transcatheter closure because of pulmonary arteriovenous fistula by magnetic resonance angiography (MRA). He had a history of occasional chest pain more than one year. Angiography didn't reveal significant stenosis at coronary artery. The patient was found a continuous grade 2/6 murmur over the left upper parastenal area one month ago. Chest MRA revealed a possible left superior pulmonary arteriovenous fistula. For diagnosis and localization of the fistula, aortography and selective angiography of the intemal mammary artery was performed and presented a left internal mammary artery-to-pulmonary artery fistula. The fistula was successfully closed using an 12 mm domestic vascular plug. Chest MRA showed that the fistula disappeared at two-month follow-up 展开更多
关键词 intemal mammary artery-to-pulmonary artery fistula Transcatheter closure Vascular plug
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The Audit of Business Strategy
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《Economics World》 2017年第4期316-321,共6页
The strategy audit involves evaluation of the actual direction of a business and comparing that act to the direction required to succeed in a changing environment. A company's actual direction is the sum of what it s... The strategy audit involves evaluation of the actual direction of a business and comparing that act to the direction required to succeed in a changing environment. A company's actual direction is the sum of what it should and should not do, how well the organisation is internally aligned to support the strategy, and how viable the strategy is when compared to external market, competitor and financial realities. These categories, the internal assessment, and the external or environmental assessment, make up the major elements of a strategy audit. In this paper, it presents such a methodology which ensures an integrated process to evaluate the company's business strategy. 展开更多
关键词 STRATEGY RISK analysis EXTERNAL intemal environment RESOURCES
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Construction of recombinant adeno-associated virus vector co-expressing hVEGF_(165) and hBMP_7 gene
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作者 Zhibin Shi Xianghui Huang +3 位作者 Kunzheng Wang Xiaoqian Dang Pei Yang Pengbo Yu 《Journal of Nanjing Medical University》 2008年第4期205-210,共6页
Objective: To construct recombinant adeno-associated virus co-expressing human vascular epithelial growth factor 165(hVEGF165) and bone morphogenetic protein 7(hBMPT), measure the virus titer and verify the recom... Objective: To construct recombinant adeno-associated virus co-expressing human vascular epithelial growth factor 165(hVEGF165) and bone morphogenetic protein 7(hBMPT), measure the virus titer and verify the recombination. Methods:The AAV helper-free system was used as basis to generate recombinant AAV. The IRES sequence of plasmid plRES was cut down and subcloned into ITR/ MCS containing vector pAAV-MCS to construct recombinant plasmid pAAV-MCSa-IRES-MCSb. The hVEGF165 and hBMP7 gene was amplified by PCR and inserted into upstream MCSa and downstream MCSb respectively. Then, recombinant plasmid pAAV- hVEGF165-IRES-hBMP7, pAAV-RC and pHelper were co-transfected into AAV-293 cells to complete rAAV-hVEGF165-IRES-hBMP7 packaging. The GFP labeled rAAV-IRES-GFP was simultaneously packaged by using the parallel plasmid pAAV-IRES-hrGFP. The efficiency of AAV packaging was monitored under fluorescent microscope and recombinant viral particles were harvested from infected AAV-293 cells. The virus titer was measured by infecting AAV-HT1080 cells, and the recombinant AAV-hVEGF165-IRES-hBMP7 was verified by PCR of the exogenous interest genes. Results:Recombinant pAAV-hVEGF165-IRES-hBMP7 was verified by double digestion. GFP expression in AAV-293 could be observed under fluorescent microscope 72 h after transfection and the system provided a high packing ratio of 95%. The recombinant adeno-associated virus has a high titer of 5.5 ×10^11vp/ml, and AAV-HT 1080 was infected at a ratio of 90%. The recombinant virus was confirmed by PCR of exogenous hBMP7 and hVEGF165 gene. Conclusion:Recombinant rAAV-hVEGF165-IRES-hBMP7 was successfully constructed with a high virus titer, which may offer foundation for in vitro and in vivo experiments of hVEGF165 and hBMP7 co-expression and provide a new method for gene therapy of bone regeneration. 展开更多
关键词 adeno-associated virus human vascular endothelial factor human bone morphogenetic protein intemal ribosome entry site
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The Effectiveness of Internal Communication in Media Organization
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作者 Ewa Jaska Agnieszka Werenowska 《Economics World》 2014年第2期137-146,共10页
A well-organized system of internal communication is vital in any organization open to improvement and change, pursuing better management of its resources and caring forgood work atmosphere. Internal communication is ... A well-organized system of internal communication is vital in any organization open to improvement and change, pursuing better management of its resources and caring forgood work atmosphere. Internal communication is primarily to provide efficient, mutual flow of information between employees and management staff at all levels in each area of the organization. In order to present internal communication tools and evaluate their effectiveness a survey study was conducted among the employees of the listed media company Cyfrowy Polsat, one of the two leading private TV groups in Poland. The study involved 136 employees (17% of total employment). The majority of respondents were male (85%). All the respondents were 23 to 47 years of age. Almost half were below 24 years of age and 38% represented the age group 25-34 years of age. The majority of respondents declared higher education with master degree and working time exceeding three years. The survey showed that the dominant form of communication is message distribution via electronic channels which confirmed the research assumption that there was a dehumanization of contacts and indirect communication dominated over direct contact. Limited direct contact was also recognized as the major cause of conflicts and disputes. The study was based on the analysis of literature and source materials (reports, statistics, newsletters, and legal acts). Additionally, the paper provides the presentation of Cyfrowy Polsat's position in the Polish media market as well as the strategies and objectives of the company. 展开更多
关键词 intemal communication media organization direct communication indirect communication forms of communication media market
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Facebook in the External Communication of High Schools
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作者 Peter Mikulas 《Journalism and Mass Communication》 2012年第9期939-945,共7页
The study is based on the i^dea of intersection of two current trends: The increasing importance of social networking services on the one hand, and on the other hand, the increase in the high schools' need to be suc... The study is based on the i^dea of intersection of two current trends: The increasing importance of social networking services on the one hand, and on the other hand, the increase in the high schools' need to be successful on the (school) market. Facebook, the most popular social networking service currently, is gradually transforming its form from the leisure and entertainment media towards the more serious media suitable for many traditional types of messages. At the same time, high schools, in addition to traditional forms of reaching out the public, are trying to find and use new forms of communication media, including social networking services. In this study, the author will focus on the current status of the social networking service----Facebook, used as a communication tool of high schools in Slovakia. The presented data are based on exploratory research followed by discursive analysis. 展开更多
关键词 external and intemal communication of high schools social media FACEBOOK
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Internal Variable Formulations of Static and Dynamic Elastic-Plastic Problems Using Liapunov Functions: Algorithmic Aspects
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作者 Modify Andrew Elton Kaunda 《Journal of Mechanics Engineering and Automation》 2011年第2期147-162,共16页
The fundamental problem of an elastic-plastic body subjected to incremental loading is reviewed using a compact internal variable approach based on work carried out at the University of Cape Town in which a quadratic ... The fundamental problem of an elastic-plastic body subjected to incremental loading is reviewed using a compact internal variable approach based on work carried out at the University of Cape Town in which a quadratic functional was developed for the free energy using Taylor series. Now the departure from that approach is the focus on developing the Liapunov function for the nonlinear differential equations of motion. Static and dynamic equations of motion are derived and shown to meet the requirements of the Liapunov function. As a consequence, time integration parameters that are used in the discrete formulations are easily obtained based on the same requirements. The resulting generalized Newton-Raphson scheme is stable in the sense of Liapunov's direct method. 展开更多
关键词 Elastic-plastic problems intemal variables forward-backward difference scheme Liapunov function generalizedNewton-Raphson scheme.
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