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硼替佐米对K562细胞株粘附分子ICAM-1表达的影响
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作者 陆世丰 陆化 +4 位作者 刘澎 王永韧 王丽霞 张建富 李建勇 《中国误诊学杂志》 CAS 2007年第27期6472-6474,共3页
目的:探讨蛋白酶体抑制剂硼替佐米(万珂,Valcade)对白血病K562细胞株细胞问粘附分子(ICAM-1)表达的影响。方法:用含10%FBS的RPMI1640培养液体外培养K562细胞,分别用0、10、20、30、50、100 nmol/L的硼替佐米干预K562细胞6h后收集,用反... 目的:探讨蛋白酶体抑制剂硼替佐米(万珂,Valcade)对白血病K562细胞株细胞问粘附分子(ICAM-1)表达的影响。方法:用含10%FBS的RPMI1640培养液体外培养K562细胞,分别用0、10、20、30、50、100 nmol/L的硼替佐米干预K562细胞6h后收集,用反转录聚合酶链反应(RT-PCR)分析K562细胞ICAM-1表达的变化。并用20 nmol/L浓度硼替佐米作用K562细胞株不同时间(0,6,12,24,48h)分析K562细胞ICAM-1表达的变化。结果:硼替佐米明显抑制K562细胞ICAM-1的表达(P<0.05),在硼替佐米浓度为0~20 nmol/L时成正比关系,当浓度>20 nmol/L各组差异无显著性。以20 nmol/L浓度作用K562细胞株不同时间后,ICAM-1的表达较作用前明显下降.并且这种效应持续存在。结论:K562细胞株ICAM-1基因表达异常增高,硼替佐米可抑制其表达量。 展开更多
关键词 硼酸化物/药理学 吡嗪类/药理学 抗肿瘤药/治疗应用 胞间粘附分子1/药物作用/代谢 K562细胞/药物作用/代谢 白血病/药物疗法 体外研究
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Advances in Experimental Studies on Treatment of Psoriasis by Traditional Chinese Medicine Zhou Yongsheng 被引量:2
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作者 张洪儒 瞿幸 周永生 《Journal of Traditional Chinese Medicine》 SCIE CAS CSCD 2002年第1期61-66,共6页
Traditional Chinese medicine has demonstrated its strength in treating psoriasis, which is characterized by a variety of methods of treatment, flexible use of drugs, high efficacy, low recurrence, and few side-effects... Traditional Chinese medicine has demonstrated its strength in treating psoriasis, which is characterized by a variety of methods of treatment, flexible use of drugs, high efficacy, low recurrence, and few side-effects. Experimental studies on the mechanism governing the TCM treatment of psoriasis have been conducted at the cellular level and the molecular level. The studies on proliferation and differentiation of keratinocytes and the inhibitory effect against them, apoptosis, intercellular adhesion molecules, cytokine, and haemorheology indicate that the laboratory studies on the treatment of psoriasis have now entered a very high stage since the eighties. The studies on relationship between typing of psoriasis based on TCM differentiation of symptoms and signs and changes in some laboratory indexes have been carried out by some researchers to explore the inherent pathological changes in typing of psoriasis based on TCM differentiation of symptoms and signs and to provide scientific basis for the differentiation and typing under a unified standard. However, the present studies lack depth and scope in the methods. In future, the laboratory studies should be enhanced to go further into the principles governing the TCM treatment of psoriasis and to seek new drugs that will be even more effective for psoriasis and can bring its relapse under control. 展开更多
关键词 ANIMALS Cell Differentiation Cell Division drug Combinations drugs Chinese Herbal Humans intercellular adhesion molecule-1 KERATINOCYTES PSORIASIS
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葛根素对大鼠脑缺血/再灌注损伤缺血区细胞粘附分子ICAM-1的影响 被引量:6
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作者 涂强 林捷 +3 位作者 谭小平 马兰 成祥林 朱遂强 《慢性病学杂志》 2010年第8期782-784,共3页
目的探讨葛根素对大鼠脑缺血/再灌注损伤缺血区细胞粘附分子ICAM-1的影响。方法采用模拟临床上缺血性卒中的大鼠中脑动脉阻塞局灶性脑缺血/再灌注模型,预先给予葛根素腹腔注射7d后,运用免疫组织化学方法 ,检测大鼠脑缺血/再灌注损伤脑... 目的探讨葛根素对大鼠脑缺血/再灌注损伤缺血区细胞粘附分子ICAM-1的影响。方法采用模拟临床上缺血性卒中的大鼠中脑动脉阻塞局灶性脑缺血/再灌注模型,预先给予葛根素腹腔注射7d后,运用免疫组织化学方法 ,检测大鼠脑缺血/再灌注损伤脑组织中ICAM-1蛋白的表达。结果葛根素能明显降低脑组织缺血区血管内皮细胞ICAM-1蛋白的表达,与模型组相比差异有统计学意义(P<0.01)。结论葛根素能明显抑制大鼠局灶性脑缺血/再灌注所致炎症反应,对大鼠脑缺血-再灌注造成的脑血管内皮损伤具有明显保护作用。 展开更多
关键词 葛根素/治疗应用/药理学 脑缺血/药物疗法 再灌注损伤/药物疗法 胞间粘附分子1/代谢/药物作用
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