The rs10954213 polymorphism and the haplotype diversity in interferon regulatory factor 5 （1RF5） play a special role in systemic lupus erythematosus （SLE） but with inconclusive results. We conducted a meta-analysi...The rs10954213 polymorphism and the haplotype diversity in interferon regulatory factor 5 （1RF5） play a special role in systemic lupus erythematosus （SLE） but with inconclusive results. We conducted a meta-analysis integrating case-control and haplotype variant studies in multiple ethnic populations to clearly discern the effect of these two variants on SLE. Eleven studies on the relation between rs10954213 polymorpisms in IRF5 and SLE were included and we selected a random effect model to calculate the pooled odds ratios （ORs） and the corresponding 95% confidence interval （95% CI）. A total of 6982 cases and 8077 controls were involved in the meta-analysis. The pooled results in- dicated that A allele was significantly associated with increased risk of SLE as compared with the IRF5 rS10954213 G allele （A vs. G, P〈0.00001） in all subjects. The same pattern of the results was also ob- tained in the European, African American, and Latin American. Asian population had a much lower prevalence of the A allele （49.1%） than any other population studied, and Europeans had the highest frequency of the IRF5 rs10954213 A allele （62.1%）. The significant association of increased SLE risk and TCA haplotype was indicated in the contrast of TCA vs. TTA as the pooled OR was 2.14 （P=0.002）. The same result was also found in the contrast of TCA vs. TTG as the pooled OR was 1.45 （P=-0.004）. This meta-analysis suggests that the A allele of rs10954213 and TCA haplotype （rs2004640-rs2070197-rs10954213） in IRF5 is associated with the increased risk of SLE in different ethnic groups, and its prevalence is ethnicity dependent.展开更多
目的:探讨系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMCs)中干扰素诱导基因AIM2的表达及其与疾病活动度的相关性。方法:运用半定量逆转录聚合酶链反应(RT-PCR)技术检测30例SLE患者(SLE组)与25例健康体检者(正常对照组)的AIM2 m RNA表...目的:探讨系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMCs)中干扰素诱导基因AIM2的表达及其与疾病活动度的相关性。方法:运用半定量逆转录聚合酶链反应(RT-PCR)技术检测30例SLE患者(SLE组)与25例健康体检者(正常对照组)的AIM2 m RNA表达水平,并且分析它们与尿蛋白、补体C3、补体C4、抗ds-DNA抗体及SLE疾病活动指数(SLEDAI积分)的相关性。结果:1与正常对照组比较,SLE组AIM2 m RNA表达显著增高(P<0.01);2 SLE组尿蛋白阳性患者的AIM2 m RNA表达水平较尿蛋白阴性患者及正常对照组显著增高(P<0.01),尿蛋白阴性组AIM2 m RNA表达水平与正常对照组比较,差异无统计学意义(P>0.05);3 SLE组AIM2 m RNA表达水平与补体C3呈显著负相关(r=-0.623,P<0.05),与抗ds-DNA抗体、SLEDAI积分呈显著正相关(r=0.621、r=0.503,P<0.05),与补体C4不相关(r=-0.426,P>0.05)。结论:SLE患者AIM2 m RNA的表达水平显著增高,并与肾脏损伤、疾病活动度相关。展开更多
目的探讨系统性红斑狼疮(system ic lupus erythem atosus,SLE)患者CD4+T细胞mRNA中干扰素诱导蛋白30基因(IP-30)的表达水平与SLE疾病活动度的相关性。方法收集23例SLE患者和10例正常对照人群的临床资料。取外周血用免疫磁珠法分离出CD...目的探讨系统性红斑狼疮(system ic lupus erythem atosus,SLE)患者CD4+T细胞mRNA中干扰素诱导蛋白30基因(IP-30)的表达水平与SLE疾病活动度的相关性。方法收集23例SLE患者和10例正常对照人群的临床资料。取外周血用免疫磁珠法分离出CD4+T细胞,抽提RNA并逆转录合成cDNA,运用GLG I方法从LongSAGE标签库中筛选出IP-30,比较该基因在系统性红斑狼疮疾病活动指数(SLEDAI)不同的SLE患者中表达的差异。结果①SLE患者组IP-30的表达量显著高于正常对照(P=0.01,P<0.05)。SLEDAI≥10组和SLEDAI<10组其IP-30的表达量显著高于正常对照(P分别为0.01和0.047),但两组间比较无显著差别(P=0.149)。②SLE有狼疮肾炎组和SLE无狼疮肾炎组相比IP-30表达量有差异(P<0.05)。③IP-30的表达量随SLE的活动水平升高而明显增加,与SLEDAI具有显著的正相关(r=0.830,P<0.01),与补体C3和外周血白细胞数成负相关(r=-0.517,r=-0.424,P<0.05)。结论CD4+T细胞IP-30水平表达升高提示IP-30可能参与SLE的发病,并对SLE活动度的判断和狼疮肾炎的诊断有一定的意义。展开更多
基金supported by the Program for New Century Excellent Talents from the Ministry of Education of China (No.NCET-09-0390)
文摘The rs10954213 polymorphism and the haplotype diversity in interferon regulatory factor 5 （1RF5） play a special role in systemic lupus erythematosus （SLE） but with inconclusive results. We conducted a meta-analysis integrating case-control and haplotype variant studies in multiple ethnic populations to clearly discern the effect of these two variants on SLE. Eleven studies on the relation between rs10954213 polymorpisms in IRF5 and SLE were included and we selected a random effect model to calculate the pooled odds ratios （ORs） and the corresponding 95% confidence interval （95% CI）. A total of 6982 cases and 8077 controls were involved in the meta-analysis. The pooled results in- dicated that A allele was significantly associated with increased risk of SLE as compared with the IRF5 rS10954213 G allele （A vs. G, P〈0.00001） in all subjects. The same pattern of the results was also ob- tained in the European, African American, and Latin American. Asian population had a much lower prevalence of the A allele （49.1%） than any other population studied, and Europeans had the highest frequency of the IRF5 rs10954213 A allele （62.1%）. The significant association of increased SLE risk and TCA haplotype was indicated in the contrast of TCA vs. TTA as the pooled OR was 2.14 （P=0.002）. The same result was also found in the contrast of TCA vs. TTG as the pooled OR was 1.45 （P=-0.004）. This meta-analysis suggests that the A allele of rs10954213 and TCA haplotype （rs2004640-rs2070197-rs10954213） in IRF5 is associated with the increased risk of SLE in different ethnic groups, and its prevalence is ethnicity dependent.
文摘目的:探讨系统性红斑狼疮(SLE)患者外周血单个核细胞(PBMCs)中干扰素诱导基因AIM2的表达及其与疾病活动度的相关性。方法:运用半定量逆转录聚合酶链反应(RT-PCR)技术检测30例SLE患者(SLE组)与25例健康体检者(正常对照组)的AIM2 m RNA表达水平,并且分析它们与尿蛋白、补体C3、补体C4、抗ds-DNA抗体及SLE疾病活动指数(SLEDAI积分)的相关性。结果:1与正常对照组比较,SLE组AIM2 m RNA表达显著增高(P<0.01);2 SLE组尿蛋白阳性患者的AIM2 m RNA表达水平较尿蛋白阴性患者及正常对照组显著增高(P<0.01),尿蛋白阴性组AIM2 m RNA表达水平与正常对照组比较,差异无统计学意义(P>0.05);3 SLE组AIM2 m RNA表达水平与补体C3呈显著负相关(r=-0.623,P<0.05),与抗ds-DNA抗体、SLEDAI积分呈显著正相关(r=0.621、r=0.503,P<0.05),与补体C4不相关(r=-0.426,P>0.05)。结论:SLE患者AIM2 m RNA的表达水平显著增高,并与肾脏损伤、疾病活动度相关。
