目的通过证据图描述和总结白介素-6(IL-6)/白介素-6受体(IL-6R)与多种健康结局孟德尔随机化研究中的证据,明确其分布,以期为该领域研究提供参考。方法系统检索PubMed、EMBASE、Web of Science、Scopus、中国知识基础设施工程、中国生物...目的通过证据图描述和总结白介素-6(IL-6)/白介素-6受体(IL-6R)与多种健康结局孟德尔随机化研究中的证据,明确其分布,以期为该领域研究提供参考。方法系统检索PubMed、EMBASE、Web of Science、Scopus、中国知识基础设施工程、中国生物医学文献服务系统、万方数据库、维普数据库中IL-6/IL-6R与多种疾病的孟德尔随机化研究,采用图表结合形式综合分析和展示纳入研究的基本信息、研究方法和研究结局。结果最终纳入38篇孟德尔随机化研究,2020年以后相关研究数增加趋势明显,纳入研究探讨IL-6、IL-6R、sIL-6R、gp130、IL-6RA 5种不同的表型和65种不同疾病间的因果关系。其中,遗传预测的IL-6水平与终末期肾病、精神分裂症、冠状动脉疾病和心房颤动存在因果关系,IL-6R与黑色素瘤、髓系白血病、膀胱癌、喉癌、眼癌和胆囊癌的发生率存在因果关系,sIL-6R水平与肺动脉高压、哮喘、抑郁、多发性硬化症、腹主动脉瘤等疾病患病率存在因果关系。结论孟德尔随机化研究提供了关于IL-6/IL-6R在多种健康结局中的因果作用的重要证据,涵盖精神心理、炎症、癌症和心血管疾病等,反映了IL-6/IL-6R在多种疾病中的广泛影响和IL-6领域研究的重要性。展开更多
AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients un...AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients undergoing coronary artery bypass grafting.Cultured cells were treated with either TNC(0.1μmol/L,24 h)or a recombinant protein corresponding to different domains of the TNC protein;fibrinogen-like globe(FBG)and fibronectin typeⅢ-like repeats(TNⅢ5-7)(both 1μmol/L,24 h).The expression of the proinflammatory cytokines;interleukin(IL)-6,IL-1β,TNFαand the matrix metalloproteinases;MMPs(MMP1,2,3,9,10,MT1-MMP)was assessed using real time RT-PCR and western blot analysis.RESULTS:TNC increased both IL-6 and MMP3(P<0.01)mR NA levels in cultured human CMF but had no significant effect on the other markers studied.The increase in IL-6 mR NA expression was mirrored by an increase in protein secretion as assessed by enzymelinked immunosorbant assay(P<0.01).Treating CMF with the recombinant protein FBG increased IL-6mR NA and protein(P<0.01)whereas the recombinant protein TNⅢ5-7 had no effect.Neither FBG nor TNⅢ5-7 had any significant effect on MMP3 expression.The expression of toll-like receptor 4(TLR4)in human CMF was confirmed by real time RT-PCR,western blot and immunohistochemistry.Pre-incubation of cells with TLR4neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mR NA and protein expression.CONCLUSION:TNC up-regulates IL-6 expression in human CMF,an effect mediated through the FBG domain of TNC and via the TLR4 receptor.展开更多
文摘目的通过证据图描述和总结白介素-6(IL-6)/白介素-6受体(IL-6R)与多种健康结局孟德尔随机化研究中的证据,明确其分布,以期为该领域研究提供参考。方法系统检索PubMed、EMBASE、Web of Science、Scopus、中国知识基础设施工程、中国生物医学文献服务系统、万方数据库、维普数据库中IL-6/IL-6R与多种疾病的孟德尔随机化研究,采用图表结合形式综合分析和展示纳入研究的基本信息、研究方法和研究结局。结果最终纳入38篇孟德尔随机化研究,2020年以后相关研究数增加趋势明显,纳入研究探讨IL-6、IL-6R、sIL-6R、gp130、IL-6RA 5种不同的表型和65种不同疾病间的因果关系。其中,遗传预测的IL-6水平与终末期肾病、精神分裂症、冠状动脉疾病和心房颤动存在因果关系,IL-6R与黑色素瘤、髓系白血病、膀胱癌、喉癌、眼癌和胆囊癌的发生率存在因果关系,sIL-6R水平与肺动脉高压、哮喘、抑郁、多发性硬化症、腹主动脉瘤等疾病患病率存在因果关系。结论孟德尔随机化研究提供了关于IL-6/IL-6R在多种健康结局中的因果作用的重要证据,涵盖精神心理、炎症、癌症和心血管疾病等,反映了IL-6/IL-6R在多种疾病中的广泛影响和IL-6领域研究的重要性。
文摘AIM:To investigate the effect of Tenascin C(TNC)on the expression of pro-inflammatory cytokines and matrixmetalloproteinases in human cardiac myofibroblasts(CMF).METHODS:CMF were isolated and cultured from patients undergoing coronary artery bypass grafting.Cultured cells were treated with either TNC(0.1μmol/L,24 h)or a recombinant protein corresponding to different domains of the TNC protein;fibrinogen-like globe(FBG)and fibronectin typeⅢ-like repeats(TNⅢ5-7)(both 1μmol/L,24 h).The expression of the proinflammatory cytokines;interleukin(IL)-6,IL-1β,TNFαand the matrix metalloproteinases;MMPs(MMP1,2,3,9,10,MT1-MMP)was assessed using real time RT-PCR and western blot analysis.RESULTS:TNC increased both IL-6 and MMP3(P<0.01)mR NA levels in cultured human CMF but had no significant effect on the other markers studied.The increase in IL-6 mR NA expression was mirrored by an increase in protein secretion as assessed by enzymelinked immunosorbant assay(P<0.01).Treating CMF with the recombinant protein FBG increased IL-6mR NA and protein(P<0.01)whereas the recombinant protein TNⅢ5-7 had no effect.Neither FBG nor TNⅢ5-7 had any significant effect on MMP3 expression.The expression of toll-like receptor 4(TLR4)in human CMF was confirmed by real time RT-PCR,western blot and immunohistochemistry.Pre-incubation of cells with TLR4neutralising antisera attenuated the effect of both TNC and FBG on IL-6 mR NA and protein expression.CONCLUSION:TNC up-regulates IL-6 expression in human CMF,an effect mediated through the FBG domain of TNC and via the TLR4 receptor.
文摘目的 探究芪地桃蛭二蝉方联合他克莫司治疗膜性肾病的效果及对抗磷脂酶A2受体(PLA2R)、B淋巴细胞活化因子(BAFF)及白细胞介素-6(IL-6)水平的影响。方法 选取2017年11月至2020年4月收治的84例膜性肾病患者为研究对象,将其随机分为对照组与联合组,各42例。对照组给予基础治疗加他克莫司治疗,联合组在对照组基础上加芪地桃蛭二蝉方治疗。比较两组的治疗效果。结果 联合组的治疗总有效率高于对照组,差异具有统计学意义(P<0.05)。治疗前,两组的24 h尿蛋白定量(24 h UPQ)、人血清白蛋白(HSA)、血清肌酐浓度(Scr)水平比较,差异无统计学意义(P>0.05);治疗后,两组的24 h UPQ、Scr水平均明显降低,HSA水平均明显升高,且联合组优于对照组,差异具有统计学意义(P<0.05)。治疗前,两组的PLA2R、BAFF及IL-6水平比较,差异无统计学意义(P>0.05);治疗后,两组的PLA2R、BAFF及IL-6水平均明显降低,且联合组低于对照组,差异具有统计学意义(P<0.05)。联合组的不良反应总发生率低于对照组,差异具有统计学意义(P<0.05)。结论 芪地桃蛭二蝉方联合他克莫司治疗膜性肾病效果显著,能够明显降低PLA2R、BAFF及IL-6水平,安全可靠,值得推广。
