Moderating effect of synthesized docosahexaenoic acid-enriched phosphatidylcholine on production of Th1 and Th2 cytokine in lipopolysaccharide-induced inflammation

Objective: To evaluate effects of docosahexaenoic acid-enriched phosphatidylcholine(DHAPC) on cytokine production induced by lipopolysaccharide(LPS). Methods: The culture supernatants of splenocytes exposed to DHA-PC along with LPS were harvested to determine the production of Th 1(IFN-kines. Cytokines were m毭 and IL-2) and Th2 [IL-4, IL-5, IL-6 and IL-12/IL-23(p40)] cytoeasured using ELISA. Results: Co-administration of DHAPC with LPS resulted in significantly lower IL-2 expression compared to that observed with administration of only LPS(P<0.01). Treatment with DHA-PC and LPS significantly increased IL-5 expression(P<0.01). Moreover, co-administration of DHA-PC with LPS significantly decreased IL-6 and IL-12/IL-23(p40) expressions compared to that observed with administration of only LPS(P<0.01). Conclusions: Our results suggest that DHA-PC inhibits pro-inflammatory cytokines [IL-2, IL-6 and IL-12/IL-23(p40)] expression on induction of inflammation.

微小核糖核酸-10a在炎症性肠病中的表达及其临床意义

目的研究微小RNA（miRNA）-10a在IBD患者肠黏膜、血清及外周血单个核细胞（PBMC）中的表达，探讨其在疾病发生过程中的作用及意义。方法收集9例活动期UC患者、11例活动期CD患者及8名肠镜无异常者的内镜肠黏膜活组织检查标本，12例活动期UC患者、13例活动期CD患者及9名健康对照者的血清标本，9例活动期UC患者、11例活动期CD患者及8名健康对照者的PBMC标本，采用荧光定量PCR技术检测肠黏膜内、血清及PBMC中miRNA10a表达以及肠黏膜内IL-12／IL-23p40表达。收集活动期UC和CD患者各8例，取英夫利昔（IFX）治疗前和经IFX治疗3次后6周的肠黏膜检测miRNA-10a表达。另收集同期的11例活动期UC患者和10例活动期CD患者的肠黏膜活组织检查标本在体外使用IFX刺激培养18h，检测肠黏膜细胞miRNA-10a表达。三样本比较采用单因素方差分析，两样本采用配对t检验，相关性分析采用Spearman检验。结果与健康对照组相比，UC及CD患者肠黏膜组织、血清、PBMC中miRNA-10a表达显著降低（F=38．45、30．46、14．74，P均〈0．05），UC及CD组间的表达量差异无统计学意义（P〉0．05），UC及CD患者肠黏膜组织IL-12／IL-23p40表达明显升高（F=32．90，P〈O．05）。CD患者结肠黏膜中IL-12／IL-23p40与miRNA-10a表达呈负相关（r=-0．8545，P〈0．01）。经IFX治疗3次后，IBD患者肠黏膜miRNA10a表达明显升高（t=3．341、3．382，P均〈0．05）。IFX体外刺激后肠黏膜miRNA-10a表达明显升高（t=3．095、7．193，P均〈0．05）。结论miRNA-10a与IBD患者炎性反应密切相关，其对IL-12／IL-23p40有靶向作用，miRNA-10a可能成为IBD治疗新靶点。