Correlation between single nucleotide polymorphism of rs3811047 in IL-1 F7 gene and rheumatoid arthritis susceptibility among Han population in central plains of China

Objective:To discuss the association between single nucleotide polymorphism(SNP) of rs3811047 in IL-1 F7 gene and rheumatoid arthritis(RA) susceptibility among the Han population in central plains of China.Methods:A total of 276 RA patients admitted to our hospital from December 2009 to December 2011 together with 276 healthy physical examinees in the same period were chosen as the subjects.The typing for rs3811047 SNP in IL-1 F7 gene was carried out by using ligase detection reaction and polymerase chain reaction technique.And the frequency of each allele and genotypes distribution was calculated so as to evaluate the association between genotype distribution and RA susceptibility.Results:The frequency of A allele of rs3811047 in IL-1 F7 gene in RA group and control group was 16.27%and 17.68%,respectively,and that of G allele in two groups was 83.73%and 82.32%,respectively.The difference between two groups wasn’t statistical significant(P 】0.05).The frequency of genotype AA,AG and GG in RA group was 2.19%,27.84%and 69.97%,respectively,while that in control group was 2.94%,29.78%and 67.28%, respectively.The difference of distribution of three genotypes was not statistically significant (P 】0.05).RA patients with A allele were better than those without A allele in joint swelling index, rest pain,HAQ scoring and blood sedimentation.There was significant difference between two groups in above indexes(P【0.05/P【0.01).Conclusions:No significant correlation between RA susceptibility among the Han population in central plains of China and rs3811047 SNP inIL-1 F7 gene is observed.However,A allele of rs3811047 has certain influence on the condition of RA patients.

白细胞介素-1F7基因单核苷酸多态性对强直性脊柱炎易感性与病情的影响

目的探讨白细胞介素（IL）-1F7基因rs3811047位点单核苷酸多态性（SNP）对强直性脊柱炎（AS）易感性和临床表现型的影响。方法收集As患者158例和同期健康献血人群181名，采用连接酶检测反应（LDR—PCR）方法检测IL-1F7基因rs3811047位点SNP，分析其等位基因频率及基因型频率在AS和对照组中的分布，并比较不同基因型AS患者间临床表现型的差别。结果AS患者和对照人群中rs3811047位点A等位基因频率（12．03％，17．68％）和G等位基因频率（87．97％，82．32％）的分布差异有统计学意义（X^2=4．2204，P=0．0399）；AA，AG，GG基因型频率在As中分别为0，24．05％，75．95％，与对照组分布（2．76％，29．83％，67．41％）相比，差异亦有统计学意义（X^2=6．2675，P=0．043）。AG基因型的As患者中人类白细胞抗原（HLA）-B27阳性率为70．27％（26／37），明显低于GG基因型AS中HIA—B27的阳性率94．23％（98／104），差异有统计学意义（x^2=2．168，P=0．030）；其红细胞沉降率和C反应蛋白水平明显亦低于GG基因型组（t=2．971，P=0．013；t=3.300，P=0．001）。结论安徽籍汉族人群AS易感性与IL-1F7基因rs3811047位点SNP有关，其基因型对AS的临床表现型有影响，携带A等位基因患者的炎症表现轻于不携带A等位基因的患者。