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Role of Activator Protein-1 in the Transcription of Interleukin-5 Gene Regulated by Protein Kinase C Signal in Asthmatic Human TLymphocytes 被引量:2
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作者 郭琦 徐永健 张珍祥 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2005年第2期147-150,共4页
Summary: In order to explore the role of activator protein-1 (AP-1) in the transcription of interleukin-5 (IL-5) gene regulated by protein kinase C (PKC) signal in peripheral blood T lymphocytes from asthmatic patient... Summary: In order to explore the role of activator protein-1 (AP-1) in the transcription of interleukin-5 (IL-5) gene regulated by protein kinase C (PKC) signal in peripheral blood T lymphocytes from asthmatic patient, T lymphocytes were isolated and purified from peripheral blood of each asthmatic patient. The T lymphocytes were randomly divided into 4 groups: group A (blank control), group B (treated with PKC agonist phorbol 12-myristate 13-acetate (PMA)), Group C (treated with PMA and AP-1 cis-element decoy oligodeoxynucleotides (decoy ODNs)), and group D (treated with PMA and AP-1 mutant decoy ODNs). The ODNs were transfected into the T cells of group C and D by cation liposome respectively. Reverse transcription-polymerase chain reaction (RT-PCR) was employed to assess IL-5 mRNA expression, and electrophoretic mobility shift assays (EMSA) for the activation of AP-1. The results showed that the activation of AP-1 (88 003.58±1 626.57) and the expression of IL-5 mRNA (0.8300±0.0294) in T lymphocytes stimulated with PMA were significantly higher than these in blank control (20 888.47±1103.56 and 0.3050±0.0208, respectively, P< 0.01), while the indexes (23 219.83±1 024.86 and 0.3425±0.0171 respectively) of T lymphocytes stimulated with PMA and AP-1 decoy ODNs were significantly inhibited, as compared with group B (P< 0.01). The indexes (87 107.41±1 342.92 and 0.8225±0.0222, respectively) in T lymphocytes stimulated with PMA and AP-1 mutant decoy ODNs did not exhibit significant changes, as compared with group B (P>0.05). The significant positive correlation was found between the activation of AP-1 and the expression of IL-5 mRNA (P< 0.01). It was concluded that AP-1 might participate in the signal transduction of PKC-triggered transcription of IL-5 gene in asthmatic T lymphocytes. This suggests the activation of PKC/AP-1 signal transduction cascade of T lymphocytes may play an important role in the pathogenesis of asthma. 展开更多
关键词 protein kinase C activator protein-1 signal transduction bronchial asthma interleukin-5 cis-element decoy oligodeoxynucleotides
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Suppressing high mobility group box-1 release alleviates morphine tolerance via the adenosine5'-monophosphate-activated protein kinase/heme oxygenase-1 pathway
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作者 Tong-Tong Lin Chun-Yi Jiang +10 位作者 Lei Sheng Li Wan Wen Fan Jin-Can Li Xiao-Di Sun Chen-Jie Xu Liang Hu Xue-Feng Wu Yuan Han Wen-Tao Liu Yin-Bing Pan 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第9期2067-2074,共8页
Opioids,such as morphine,are the most potent drugs used to treat pain.Long-term use results in high tolerance to morphine.High mobility group box-1(HMGB1) has been shown to participate in neuropathic or inflammatory p... Opioids,such as morphine,are the most potent drugs used to treat pain.Long-term use results in high tolerance to morphine.High mobility group box-1(HMGB1) has been shown to participate in neuropathic or inflammatory pain,but its role in morphine tolerance is unclear.In this study,we established rat and mouse models of morphine tolerance by intrathecal injection of morphine for 7 consecutive days.We found that morphine induced rat spinal cord neurons to release a large amount of HMGB1.HMGB1 regulated nuclear factor κB p65 phosphorylation and interleukin-1β production by increasing Toll-like receptor 4receptor expression in microglia,thereby inducing morphine tolerance.Glycyrrhizin,an HMGB1 inhibito r,markedly attenuated chronic morphine tole rance in the mouse model.Finally,compound C(adenosine 5’-monophosphate-activated protein kinase inhibitor) and zinc protoporphyrin(heme oxygenase-1 inhibitor)alleviated the morphine-induced release of HMGB1 and reduced nuclear factor κB p65 phosphorylation and interleukin-1β production in a mouse model of morphine tolerance and an SH-SY5Y cell model of morphine tole rance,and alleviated morphine tolerance in the mouse model.These findings suggest that morphine induces HMGB1 release via the adenosine 5’-monophosphate-activated protein kinase/heme oxygenase-1 signaling pathway,and that inhibiting this signaling pathway can effectively reduce morphine tole rance. 展开更多
关键词 adenosine 5’-monophosphate-activated protein kinase heme oxygenase-1 high mobility group box-1 interleukin- MICROGLIA morphine tolerance NEUROINFLAMMATION neuron nuclear factor-κB p65 Toll-like receptor 4
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辨证贴药对过敏性哮喘患者IL-5及ECP的影响 被引量:23
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作者 李月梅 赖新生 +1 位作者 庄礼兴 江钢辉 《中国针灸》 CAS CSCD 北大核心 2002年第2期119-120,共2页
目的 :探讨穴位敷贴的治哮机制。方法 :将 2 0 9例哮喘患者随机分为 2组 ,其中辨证贴药组12 5例 ,脱敏组 84例 ,分别观察两组治疗前后白介素 5 (IL 5 )及嗜酸性阳离子蛋白 (ECP)水平。 结果 :穴位敷贴后患者的IL 5及ECP含量明显降低 ,... 目的 :探讨穴位敷贴的治哮机制。方法 :将 2 0 9例哮喘患者随机分为 2组 ,其中辨证贴药组12 5例 ,脱敏组 84例 ,分别观察两组治疗前后白介素 5 (IL 5 )及嗜酸性阳离子蛋白 (ECP)水平。 结果 :穴位敷贴后患者的IL 5及ECP含量明显降低 ,与治疗前比较差异具有显著性意义 (P <0 0 1;P<0 0 5 ) ,且辨证贴药组对IL 5的影响程度明显大于对照组 ,组间比较差异有显著意义 (P <0 0 5 )。结论 展开更多
关键词 哮喘 穴位疗法 白细胞介素5 穴位贴敷法 辩证分型
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5-氨基酮戊酸光动力疗法治疗肛周-肛管尖锐湿疣护理体会 被引量:9
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作者 王娜 杨春香 +2 位作者 王娟 王芳兰 张晓梅 《中国皮肤性病学杂志》 CAS 北大核心 2009年第11期751-752,共2页
目的探讨5-氨基酮戊酸光动力疗法治疗肛周-肛管尖锐湿疣患者的护理方法。方法对30例肛周-肛管尖锐湿疣患者进行光动力治疗,密切观察治疗各阶段的精心护理对临床疗效的影响。结果经3次治疗后27例患者皮损完全消退,恢复正常黏膜结构;3例... 目的探讨5-氨基酮戊酸光动力疗法治疗肛周-肛管尖锐湿疣患者的护理方法。方法对30例肛周-肛管尖锐湿疣患者进行光动力治疗,密切观察治疗各阶段的精心护理对临床疗效的影响。结果经3次治疗后27例患者皮损完全消退,恢复正常黏膜结构;3例肛管、肛周均受累的患者在治疗4周后复发,经再次治疗未见复发。结论加强光动力治疗前、中、后的各项护理,有助于取得较好的疗效。 展开更多
关键词 尖锐湿疣 肛周-肛管 光动力疗法 5-氨基酮戊酸 护理
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5-脂氧酶/半胱氨酰白三烯途径不参与大鼠C6胶质瘤细胞缺氧缺糖损伤 被引量:3
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作者 黄雪琴 黄晓佳 +4 位作者 张丽慧 戚玲玲 卢韵碧 张纬萍 魏尔清 《浙江大学学报(医学版)》 CAS CSCD 2008年第5期456-462,共7页
目的:观察缺氧缺糖(OGD)是否损伤大鼠C6胶质瘤细胞,以及5-脂氧酶(5-LOX)/半胱氨酰白三烯(CysLT)途经是否参与OGD损伤。方法:在OGD处理并恢复不同时间后,观察C6细胞活性变化,以及5-LOX抑制剂和CysLT受体拮抗剂的影响;以免疫细胞... 目的:观察缺氧缺糖(OGD)是否损伤大鼠C6胶质瘤细胞,以及5-脂氧酶(5-LOX)/半胱氨酰白三烯(CysLT)途经是否参与OGD损伤。方法:在OGD处理并恢复不同时间后,观察C6细胞活性变化,以及5-LOX抑制剂和CysLT受体拮抗剂的影响;以免疫细胞化学法观察5-LOX蛋白的细胞内分布;以RT-PCR法检测CysLT1和CysLT2受体mRNA表达;并观察白三烯D4(LTD4)对C6细胞的作用。结果:OGD4-8h并恢复24-72h后,可诱导C6细胞损伤;5-LOX抑制剂和CysLT受体拮抗剂对OGD损伤无明显作用。OGD未诱导5-LOX的核膜移位。C6细胞高表达CysLT2受体,但CysLT1受体表达很微弱,OGD不影响它们的表达。此外,LTD4对C6细胞没有明显作用。结论:OGD可诱导C6细胞损伤,但5-LOX/CysLT途径不参与OGD诱导的损伤。 展开更多
关键词 神经胶质瘤 缺氧缺血 白三烯类/分析 白三烯B4/分析 半胱氨酸/分析 白三烯拮抗剂 花生四烯酸盐5-脂氧合酶 细胞 培养的
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哮喘患儿血清白细胞介素-5和IgE与肺功能的相关性研究 被引量:1
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作者 陈晓锐 彭俊旭 +2 位作者 安正红 郑华 李鹤 《山西医药杂志》 CAS 2014年第7期754-756,共3页
目的检测支气管哮喘患儿外周血白细胞介素(IL)-5、总IgE(TIgE)和肺功能水平,探讨IL-5、TIgE与肺功能水平及支气管哮喘之间的相关性。方法选取35例门诊随诊的哮喘缓解期患儿作为哮喘组,30名同期健康体检儿童作为健康对照组,2组儿童均于... 目的检测支气管哮喘患儿外周血白细胞介素(IL)-5、总IgE(TIgE)和肺功能水平,探讨IL-5、TIgE与肺功能水平及支气管哮喘之间的相关性。方法选取35例门诊随诊的哮喘缓解期患儿作为哮喘组,30名同期健康体检儿童作为健康对照组,2组儿童均于清晨空腹取外周静脉血1.0mL制备血清,应用双抗体夹心酶联免疫吸附试验(ELISA)法测定2组儿童血清IL-5、TIgE,并行肺功能检测。结果哮喘组患儿血清IL-5、TIgE水平均较健康对照组升高,差异有统计学意义(t=6.84、21.63,P<0.01),肺功能用力呼出25%肺活量时呼气流量(FEF25%)、用力呼出50%肺活量时呼气流量(FEF50%)哮喘组较健康对照组水平明显下降(t=-2.258、-2.428,P<0.05),而第1秒用力呼气容积占用力肺活量的比值(FEV1/FVC%)差异无统计学意义;IL-5与FEV1/FVC%、FEF25%、FEF50%无相关性,TIgE与FEV1/FVC%、FEF25%、FEF50%呈负相关(r=-0.534、-0.392、-0.394,P<0.05)。结论哮喘患儿体内存在Th1/Th2免疫失衡,IL-5和TIgE参与气道高反应与呼吸道重塑的病理生理过程,TIgE即使在哮喘缓解期也仍然和肺功能密切相关,可以成为辅助判定哮喘严重程度的参考指标。 展开更多
关键词 哮喘 白细胞介素-5 肺功能测定 儿童 interleukin-5
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Transplantation of human placental chorionic plate-derived mesenchymal stem cells for repair of neurological damage in neonatal hypoxic-ischemic encephalopathy
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作者 Lulu Xue Ruolan Du +8 位作者 Ning Bi Qiuxia Xiao Yifei Sun Ruize Niu Yaxin Tan Li Chen Jia Liu Tinghua Wang Liulin Xiong 《Neural Regeneration Research》 SCIE CAS CSCD 2024年第9期2027-2035,共9页
Neonatal hypoxic-ischemic encephalopathy is often associated with permanent cerebral palsy,neurosensory impairments,and cognitive deficits,and there is no effective treatment for complications related to hypoxic-ische... Neonatal hypoxic-ischemic encephalopathy is often associated with permanent cerebral palsy,neurosensory impairments,and cognitive deficits,and there is no effective treatment for complications related to hypoxic-ischemic encephalopathy.The therapeutic potential of human placental chorionic plate-derived mesenchymal stem cells for various diseases has been explored.However,the potential use of human placental chorionic plate-derived mesenchymal stem cells for the treatment of neonatal hypoxic-ischemic encephalopathy has not yet been investigated.In this study,we injected human placental chorionic plate-derived mesenchymal stem cells into the lateral ventricle of a neonatal hypoxic-ischemic encephalopathy rat model and observed significant improvements in both cognitive and motor function.Protein chip analysis showed that interleukin-3 expression was significantly elevated in neonatal hypoxic-ischemic encephalopathy model rats.Following transplantation of human placental chorionic plate-derived mesenchymal stem cells,interleukin-3 expression was downregulated.To further investigate the role of interleukin-3 in neonatal hypoxic-ischemic encephalopathy,we established an in vitro SH-SY5Y cell model of hypoxic-ischemic injury through oxygen-glucose deprivation and silenced interleukin-3 expression using small interfering RNA.We found that the activity and proliferation of SH-SY5Y cells subjected to oxygen-glucose deprivation were further suppressed by interleukin-3 knockdown.Furthermore,interleukin-3 knockout exacerbated neuronal damage and cognitive and motor function impairment in rat models of hypoxic-ischemic encephalopathy.The findings suggest that transplantation of hpcMSCs ameliorated behavioral impairments in a rat model of hypoxic-ischemic encephalopathy,and this effect was mediated by interleukin-3-dependent neurological function. 展开更多
关键词 behavioral evaluations gene knockout human neuroblastoma cells(SH-SY5Y) human placental chorionic derived mesenchymal stem cells interleukin-3 neonatal hypoxic-ischemic encephalopathy nerve injury oxygen-glucose deprivation protein chip small interfering RNA
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Cytotoxic effect of interleukin-8 in retinal ganglion cells and its possible mechanisms 被引量:4
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作者 Jing-Jing Wang Walana Williams +6 位作者 Bing Wang Jing Wei Xia Lu Jya-Wei Cheng John R Gordon Jing-Min Li Fang Li 《International Journal of Ophthalmology(English edition)》 SCIE CAS 2018年第8期1277-1283,共7页
AIM: To investigate the effect of interleukin-8(IL-8) on neural retinal ganglion cells(RGCs) and whether it can be alleviated by G31P. METHODS: RGC-5 cells were exposed to IL-8 with or without its specific recep... AIM: To investigate the effect of interleukin-8(IL-8) on neural retinal ganglion cells(RGCs) and whether it can be alleviated by G31P. METHODS: RGC-5 cells were exposed to IL-8 with or without its specific receptor antagonist G31P for 24h, and the cell viability was assessed by Cell Counting Kit 8(CCK-8). Apoptosis was measured by examining nuclear morphology and quantifying with flow cytometry. Reverse transcription quantitative real-time polymerase chain reaction(RT-qP CR) and Western blot were used to investigate the expression of apoptosis-related genes. RESULTS: CCK-8 assay showed that IL-8 significantly inhibits the viability of RGC-5 cells in a dose-dependent manner. Cell apoptosis assays exhibited higher apoptotic rate in IL-8 treatment group compared to control group. We further found that IL-8 could promote Bax and caspase-3 expressions, but decrease the level of Bcl-2 in the aspect of m RNA and protein. However, pre-treatment with G31P partly attenuated these effects in RGC-5 cells(P〈0.05).CONCLUSION: These results indicate that anti-proliferation effects of IL-8 through induction of cell apoptosis regulated by Bcl-2, Bax and caspase-3 expressions, can be ameliorated by G31P. 展开更多
关键词 GLAUCOMA INFLAMMATION interleukin-8 retinalganglion cell-5 APOPTOSIS G31P
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Constituents of the anti-asthma herbal formula ASHMI^(TM) synergistically inhibit IL-4 and IL-5 secretion by murine Th2 memory cells,and eotaxin by human lung fibroblasts in vitro 被引量:15
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作者 Bolleddula Jayaprakasam Nan Yang +4 位作者 Ming-Chun Wen Rong Wang Joseph Goldfarb Hugh Sampson Xiu-Min Li 《Journal of Integrative Medicine》 SCIE CAS CSCD 2013年第3期195-205,共11页
OBJECTIVE: Anti-asthma herbal medicine intervention (ASHMITM), a combination of three tradi- tional Chinese medicinal herbs developed in our laboratory, has demonstrated efficacy in both mouse models of allergic as... OBJECTIVE: Anti-asthma herbal medicine intervention (ASHMITM), a combination of three tradi- tional Chinese medicinal herbs developed in our laboratory, has demonstrated efficacy in both mouse models of allergic asthma, and a double-blind placebo-controlled clinical trial in patients with asthma. This study was designed to determine if the anti-inflammatory effects of individual herbal constituents of ASHMITM exhibited synergy. METHODS: Effects of ASHMI and its components aqueous extracts of Lingzhi (Ganoderma lucidum), Kushen (Sophora flavescens) and Gancao (Glycyrrhiza uralensis), on Th2 cytokine secretion by murine memory Th2 cells (D10.G4.1) and eotaxin-1 secretion by human lung fibroblast (HLF-1) cells were determined by measuring levels in culture supernatants by enzyme- linked immunosorbent assay. Potential synergistic effects were determined by computing interaction indices from concentration-effect curve parameters. RESULTS: Individual Lingzhi, Kushen and Gancao extracts and ASHMI (the combination of individual extracts) inhibited production of interleukin (IL)-4 and IL-5 by murine memory Th2 cells and eotaxin-1 production by HLF-1 cells. The mean 25%-inhibitory-concentration (IC2s) values (mg/mL) forASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 30.9, 79.4, 123, and 64.6, respectively; for IL-5 production were 30.2, 263, 123.2 and 100, respectively; for eotaxin-1 were 13.2, 16.2, 30.2, and 25.1, respectively. The IC50values (mg/mL) for ASHMI, Lingzhi, Kushen and Gancao for IL-4 production were 158.5, 239.9, 446.7, and 281.8, respectively; for eotaxin-1 were 38.1, 33.1, 100, and 158.5, respectively. The interaction indices of ASHMI constituents at IC25 were 0.35 for IL-4, 0.21 for IL-5 and 0.59 for eotaxin-l. The interaction indices at IC^0 values were 0.50 for IL-4 and 0.62 for eotaxin-1 inhibition. Inhibition of IL-5 did not reach IC^0 values. All interaction indices were below 1 which indicated synergy. CONCLUSION: By comparing the interaction index values, we find that constituents in ASHMITM synergistically inhibited eotaxin-1 production as well as Th2 cytokine production. 展开更多
关键词 medicine Chinese traditional medicine herbal plant extracts anti-asthma herbal medicine intervention (ASHMI) anti-asthmatic agents chemokine CCL11 interleukin-4 interleukin -5 in vitro
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肛瘘病人手术前后血清中IgA、血清淀粉蛋白A、趋化因子5和炎性因子水平变化与肛门功能及疗效的关系 被引量:14
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作者 梁艳 《临床外科杂志》 2020年第5期460-463,共4页
目的探讨肛瘘病人手术前后血清中IgA、血清淀粉蛋白A(SAA)、趋化因子5(CCL5)和炎性因子水平变化与肛门功能及疗效的关系。方法 2017年10月~2019年1月我院收治的肛瘘病人114例,所有病人均接受手术治疗。采用酶联免疫吸附法检测血清中IgA... 目的探讨肛瘘病人手术前后血清中IgA、血清淀粉蛋白A(SAA)、趋化因子5(CCL5)和炎性因子水平变化与肛门功能及疗效的关系。方法 2017年10月~2019年1月我院收治的肛瘘病人114例,所有病人均接受手术治疗。采用酶联免疫吸附法检测血清中IgA、SAA、CCL5和白细胞介素(IL)-4、IL-6及IL-10水平,比较手术前后血清中IgA、SAA、CCL5和IL-4、IL-6及IL-10炎性因子水平变化与肛门功能和手术效果的关系。结果治疗后,病人IgA、SAA、CCL5、IL-4、IL-6和IL-10水平、Wexner评分均显著下降,差异有统计学意义(P<0.05);术后IgA、SAA、CCL5、IL-4、IL-6及IL-10低水平组病人的手术效果比高水平组更好,差异有统计学意义(P<0.05);IgA、SAA、CCL5、IL-4、IL-6和IL-10与病程、Wexner评分呈正相关(P<0.05),与治疗效果呈负相关(P<0.05)。结论肛瘘病人手术后血清中IgA、SAA、CCL5和炎性因子水平均低于手术前,血清中IgA、SAA、CCL5和炎性因子水平与手术效果和病人术后肛门功能密切相关。 展开更多
关键词 肛瘘 免疫球蛋白A 血清淀粉蛋白A 趋化因子5 炎性因子 肛门功能
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The Effect of Ginkgo Biloba Extract on the Expression of PKCα in the Inflammatory Cells and the Level of IL-5 in Induced Sputum of Asthmatic Patients 被引量:2
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作者 唐以军 徐永健 +6 位作者 熊盛道 倪望 陈仕新 高宝安 叶涛 曹勇 杜春玲 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2007年第4期375-380,共6页
To investigate the effect of the Ginkgo Biloba Extract (GBE) on the asthma and examine its possible mechanisms, 75 asthma patients were divided into 4 groups and the patients were respectively treated with fluticaso... To investigate the effect of the Ginkgo Biloba Extract (GBE) on the asthma and examine its possible mechanisms, 75 asthma patients were divided into 4 groups and the patients were respectively treated with fluticasone propionate for 2 weeks or 4 weeks, or treated with fluticasone propionate plus GBE for 2 weeks or 4 weeks. Fifteen healthy volunteers served as healthy controls. Sputum inhalation with inhaling hypertonic saline (4%-5%) was performed. Lung ventilatory function and forced expiratory volume in one second (FEVI) were measured. The numbers of different cells in induced sputum were calculated. The expression of PKCα in the cells was immunocytochemically detected and the percentages of positive cells in different cells were counted. Interleukin-5 (IL-5) in sputum supernatants was detected with enzyme-linked immunosorbent assay. The percentage of eosinophils, lymphocytes, PKCα positive inflammatory cells and the concentration of IL-5 in asthmatic patients were higher than those in the controls (P〈0.05), and the eosinophils, lymphocytes, positive expression of PKCα and the level of IL-5 were significantly decreased in asthmatic patients after they were treated with fluticasone propionate or fluticasone propionate plus GBE. However, they were still significantly higher than those of the controls. Compared to the group treated with glucocorticosteroid for 2 weeks, no significant decrease was found in the percentage of eosinophils, lymphocytes, PKCα positive inflammatory cells and the IL-5 in the supernatant of induced sputum. Compared with the group treated with glucocorticosteroid for 2 or 4 weeks, significant decrease in the same parameters was observed in the group treated with fluticasone propionate and GBE for 4 weeks. The IL-5 level in the supernatant of induced sputum was positively correlated with the percentage of PKCα-positive inflammatory cells and the percentage of eosinophils in the induced sputum in asthma patient groups respectively (n=150, r=0.83, P〈0.01; n=150, r=0.76, P〈0.01). The FEVI was negatively correlated with the percentage of PKCα-positive inflammatory cells and the IL-5 levels in supernatant of induced sputum in asthma patients respectively (n=150, r=-0.77, P〈0.01; n=150, r=- 0.64, P〈0.01). It is concluded that GBE could significantly decrease the infiltration of inflammatory cells such as eosinophils and lymphocytes in the asthmatic airway and relieve the airway inflammation. GBE may decrease the activation of the PKCα in the inflammatory cells and thereby decrease the IL-5 level in induced sputum. GBE may be used as a complement to the glucocorticosteroid therapy for asthma. 展开更多
关键词 bronchial Asthma glucocorticosteroid Ginkgo Biloba Extract proteinase C interleukin-5
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(5R)-5-hydroxytriptolide inhibits the inflammatory cascade reaction in astrocytes 被引量:1
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作者 Yan-Qiu Cui Yan Zheng +3 位作者 Gui-Lian Tan Dong-Mei Zhang Jun-Ya Wang Xiao-Min Wang 《Neural Regeneration Research》 SCIE CAS CSCD 2019年第5期913-920,共8页
Many studies have shown that(5R)-5-hydroxytriptolide is the optimal modified analogue of triptolide, possessing comparable immunosuppressive activity but much lower cytotoxicity than triptolide. Whether(5R)-5-hydroxyt... Many studies have shown that(5R)-5-hydroxytriptolide is the optimal modified analogue of triptolide, possessing comparable immunosuppressive activity but much lower cytotoxicity than triptolide. Whether(5R)-5-hydroxytriptolide has preventive effects on neuroinflammation is unclear. This study was designed to pretreat primary astrocytes from the brains of neonatal Sprague-Dawley rats with 20, 100 and 500 nM(5R)-5-hydroxytriptolide for 1 hour before establishing an in vitro neuroinflammation model with 1.0 μg/mL lipopolysaccharide for 24 hours. The generation of nitric oxide was detected by Griess reagents. Astrocyte marker glial fibrillary acidic protein was measured by immunohistochemical staining. The levels of tumor necrosis factor-α and interleukin-1β in the culture supernatant were assayed by enzyme linked immunosorbent assay. Nuclear factor-κB/p65 expression was examined by immunofluorescence staining. The phosphorylation of inhibitor of nuclear factor IκB-α and the location of nuclear factor-κB/P65 were determined using western blot assay. Our data revealed that(5R)-5-hydroxytriptolide inhibited the generation of nitric oxide, tumor necrosis factor-α and interleukin-1β from primary astrocytes activated by lipopolysaccharide, decreased the positive reaction intensity of glial fibrillary acidic protein, reduced the expression of tumor necrosis factor alpha and interleukin-1β in culture supernatant, inhibited the phosphorylation of IκB-α and the translocation of nuclear factor-κB/P65 to the nucleus. These results have confirmed that(5R)-5-hydroxytriptolide inhibits lipopolysaccharide-induced glial inflammatory response and provides cytological experimental data for(5R)-5-hydroxytriptolide in the treatment of neurodegenerative diseases. 展开更多
关键词 NEUROINFLAMMATION (5R)-5-hydroxytriptolide tumor necrosis factor-α interleukin- NITRIC oxide nuclear factor-κB/P65 IΚB-Α microglia neural regeneration
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Interleukin (IL) 5 levels and eosinophilia in patients with intestinal parasitic diseases 被引量:1
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作者 Sebnem Ustun Nevin Turgay +1 位作者 Songul Bayram Delibas Hatice Ertabaklar 《World Journal of Gastroenterology》 SCIE CAS CSCD 2004年第24期3643-3646,共4页
AIM:Intestinal parasitic diseases are commonly accompanied with diarrhoeal symptoms and allergic reactions.Eosinophilia occurs as a result of IL-5 synthesized from Th2 cells during allergic reactions.IL-5 acts as a fa... AIM:Intestinal parasitic diseases are commonly accompanied with diarrhoeal symptoms and allergic reactions.Eosinophilia occurs as a result of IL-5 synthesized from Th2 cells during allergic reactions.IL-5 acts as a factor activating eosinophils. The aim of this study was to compare the IL-5 cytokine measurements in serum samples and cell cultures.And also to compare eosinophilia observed in helminth infections and protozoon infections accompanied with allergy. METHODS:Twenty-three patients who presented with diarrhoeal symptoms and allergic complaints were tested positive for intestinal parasites,as well as 21 controls with allergic complaints who did not have any intestinal parasites were included in this study.IL-5 production in in vitro cell cultures prepared by using phytohemaglutinin (PHA) to stimulate peripheral blood mononuclear cells (PBMC) obtained from the blood samples taken from these patients were compared with the IL-5 level in serum.Furthermore, the IL-5 production in protozoon and helminth infections was also compared.Absolute eosinophil values in 1 mm^3 of blood were calculated by means of peripheral smear in both groups within the scope of the study. RESULTS:Parasites such as helminth detected in 15 (65.2%) and protozoon in 8 (34.8%) of the patients were included in this study.As regards the values of the sera in both patients with parasite infection and controls,the IL-5 production was found to be higher in the cell culture supernatant (P<0.001 and P<0.05).When the IL-5 level of the patients with helminth parasites was compared with that of those with protozoon,it was determined that the IL-5 level in serum was more significant in the patients with protozoon than in those with helminth (P<0.05).In the study group,the patients were found to have parasites, the percentage of eosinophil was 7.0% compared to 6.5% in the control group.Thus,there was no significant difference between the eosinophil values (P>0.05). CONCLUSION:It was found that IL-5 cytokine levels in serum samples from the patients with helminth and protozoon displayed more measurable values as compared to the IL-5 levels after stimulation with mitogen.It is concluded that IL-5 acts as a triggering factor in the toxiallergic complaints commonly seen in helminth and protozoon infections. 展开更多
关键词 Adult Cells Cultured DIARRHEA EOSINOPHILIA Female HELMINTHIASIS Humans HYPERSENSITIVITY interleukin-5 Male Middle Aged Protozoan Infections Th2 Cells
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龙胆泻肝汤治疗慢性肛隐窝炎的临床疗效及对血清IL-6、MCP-1与SP、5-HT、CCK的影响 被引量:5
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作者 王静 张晓燕 《世界中西医结合杂志》 2020年第10期1890-1893,1897,共5页
目的探讨龙胆泻肝汤治疗慢性肛隐窝炎的临床疗效及对血清IL-6、MCP-1与SP、5-HT、CCK的影响。方法选择2018年5月—2019年7月肛肠科就诊的慢性肛隐窝炎患者120例,随机分配为A组和B组各60例,A组采用常规药物保留灌肠治疗,B组在A组治疗基... 目的探讨龙胆泻肝汤治疗慢性肛隐窝炎的临床疗效及对血清IL-6、MCP-1与SP、5-HT、CCK的影响。方法选择2018年5月—2019年7月肛肠科就诊的慢性肛隐窝炎患者120例,随机分配为A组和B组各60例,A组采用常规药物保留灌肠治疗,B组在A组治疗基础上予以龙胆泻肝汤治疗,对比两组治疗前后生活质量评分及治疗后复发情况、临床疗效。结果 B组患者社会功能、躯体功能、饮食及心理健康评分均优于A组,B组复发率低于A组(χ^2=4.230,P=0.040),差异具有统计学意义;B组治疗后有效率明显高于A组(χ^2=4.180,P=0.041),差异具有统计学意义;治疗后,两组患者血清白介素-6(IL-6)、P物质(SP)、人单核细胞趋化蛋白-1(MCP-1)、5-羟色胺(5-HT)、血清胆囊收缩素(CCK)表达水平均较本组治疗前下降,且B组低于A组(P<0.05),差异均具有统计学意义;B组不良反应发生率高于A组(χ^2=0.540,P=0.464),但差异无统计学意义。结论龙胆泻肝汤可提高慢性肛隐窝炎患者治疗效果,降低血清IL-6、MCP-1、SP、5-HT、CCK表达水平。 展开更多
关键词 龙胆泻肝汤 肛隐窝炎 白介素-6 P物质 人单核细胞趋化蛋白-1 5-羟色胺 血清胆囊收缩素
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Effects of Glucocorticoid on IL-13-induced Muc5ac Expression in Airways of Mice
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作者 刘剑波 张珍祥 +2 位作者 徐永健 邢丽华 张惠兰 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2004年第6期575-577,共3页
Summary: To study the effects of glucocorticoid on the IL-13-induced Muc5ac expression in airways of mice, and investigate its role in mucus secretion of airways, 24 pathogen-free BALB/c mice were randomly divided int... Summary: To study the effects of glucocorticoid on the IL-13-induced Muc5ac expression in airways of mice, and investigate its role in mucus secretion of airways, 24 pathogen-free BALB/c mice were randomly divided into 3 groups. IL-13 group received an nasal instillation of 100 μg of recombinant murine IL-13 solution on days 1, 3 and 5. In dexamethasone group, dexamethasone (0.5 mg/kg) was administered intraperitoneally 24 h before and 1 h before the first instillation of IL-13 and on 4 consecutive days (day 0 to day 5, 6 consecutive days in total), while control group was not treated with IL-13 or dexamethasone. Bronchoalveolar lavage fluid (BALF) was collected and eosinophils were counted, and expression of Muc5ac mRNA and protein in lungs were detected by reverse transcription-polymerase chain reaction (RT-PCR) technology and immunohistochemical assay respectively. Our results showed that the number of mice, with positve Muc5ac protein expression, expression of Muc5ac mRNA and eosinophils in BALF after IL-13 treatment were all significantly higher than that of control group ( all P<0.01). Despite eosinophils reduced (P<0.01), the number of mice with positive Muc5ac protein expression, expression of Muc5ac mRNA after dexamethasone treatment didn't decreas significantly as compared with that of IL-13 group. It is concluded that IL-13 can up-regulate the expression of Muc5ac mRNA and protein, which may play a pivotal role in the mucus overproduction of airways. Dexamethasone can suppress IL-13-induced eosinophilic infiltration in lung but can't inhibit the mucus overproduction. 展开更多
关键词 CORTICOSTEROID interleukin-13 bronchial asthma MUCIN MUC5AC
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Development of an in Vitro Assay to Evaluate the Biological Impact of 5G Technology on Human Skin—Shield Effect of a Tannin-Rich Plant Extract
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作者 Katharina Kappler Franziska Wandrey +2 位作者 Jean-Eric Branka Stéphane Poigny Fred Zülli 《Journal of Cosmetics, Dermatological Sciences and Applications》 2022年第2期100-108,共9页
Background: The new 5G telecommunication technology has stirred concerns about potential negative effects on human health by radiofrequency electromagnetic fields. As to whether skin biology can be affected by 5G wave... Background: The new 5G telecommunication technology has stirred concerns about potential negative effects on human health by radiofrequency electromagnetic fields. As to whether skin biology can be affected by 5G waves has remained an unsolved challenge despite recent studies dealing with this issue. In particular, a strategy for rational design of an assay allowing to 1) reproducibly evaluate and decipher the 5G effects on skin as well as 2) test the potential protective effects of cosmetic active ingredients, has yet to be found. Here we describe an in vitro model of human normal keratinocytes irradiated by 5G waves and show their impact on two biomarkers of inflammatory stress, i.e. interleukin-1β (IL-1β) and reactive oxygen species (ROS) production. In addition, the capacity of a tannin-rich plant extract to protect against 5G impact is evaluated. Materials and Methods: In the first series of experiments, monolayers of human normal keratinocytes were irradiated or not (control) by 5G waves (3.5 MHz) in an anechoic chamber and were incubated at 37&#730;C for 24 hours. At the end of the incubation period, extracellular IL-1β and intracellular ROS were quantified using specific ELISA and colorimetric assays, respectively. In the second series of experiments, the effect of an overnight pre-incubation with increasing concentrations of a tannin-rich plant extract was evaluated. Additionally, we studied in a prospective way the expression of a set of 88 genes selected for their relevance to keratinocyte homeostasis, in relation to the 5G challenge as well as the protective effect of a tannin-rich plant extract. Results: 5G waves significantly increased IL-1β production by 48.4% (p β and ROS production. Finally, the expression of 47 genes was modified by 5G waves and/or by the tannin-rich plant extract. Conclusion: This is to our knowledge the first evaluation of the impact of 5G technology on inflammatory biomarkers of human normal skin cells. Here we provide an innovative and pertinent tool to screen for natural compounds with protective effects against 5G waves to develop cosmetic products shielding against the potentially deleterious effects of electromagnetic waves on human skin. 展开更多
关键词 5G waves Human Normal Keratinocytes INFLAMMATION ROS interleukin-1 mRNA
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Argon preconditioning protects neuronal cells with a Toll-like receptor-mediated effect 被引量:3
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作者 Stefanie Scheid Adrien Lejarre +3 位作者 Jakob Wollborn Hartmut Buerkle Ulrich Goebel Felix Ulbrich 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第6期1371-1377,共7页
The noble gas argon has the potential to protect neuronal cells from cell death.So far,this effect has been studied in treatment after acute damage.Preconditioning using argon has not yet been investigated.In this stu... The noble gas argon has the potential to protect neuronal cells from cell death.So far,this effect has been studied in treatment after acute damage.Preconditioning using argon has not yet been investigated.In this study,human neuroblastoma SH-SY5Y cells were treated with different concentrations of argon(25%,50%,and 74%;21%O_(2),5%CO_(2),balance nitrogen)at different time intervals before inflicting damage with rotenone(20μM,4 hours).Apoptosis was determined by flow cytometry after annexin V and propidium iodide staining.Surface expressions of Toll-like receptors 2 and 4 were also examined.Cells were also processed for analysis by western blot and qPCR to determine the expression of apoptotic and inflammatory proteins,such as extracellular-signal regulated kinase(ERK1/2),nuclear transcription factor-κB(NF-κB),protein kinase B(Akt),caspase-3,Bax,Bcl-2,interleukin-8,and heat shock proteins.Immunohistochemical staining was performed for TLR2 and 4 and interleukin-8.Cells were also pretreated with OxPAPC,an antagonist of TLR2 and 4 to elucidate the molecular mechanism.Results showed that argon preconditioning before rotenone application caused a dose-dependent but not a time-dependent reduction in the number of apoptotic cells.Preconditioning with 74%argon for 2 hours was used for further experiments showing the most promising results.Argon decreased the surface expression of TLR2 and 4,whereas OxPAPC treatment partially abolished the protective effect of argon.Argon increased phosphorylation of ERK1/2 but decreased NF-κB and Akt.Preconditioning inhibited mitochondrial apoptosis and the heat shock response.Argon also suppressed the expression of the pro-inflammatory cytokine interleukin-8.Immunohistochemistry confirmed the alteration of TLRs and interleukin-8.OxPAPC reversed the argon effect on ERK1/2,Bax,Bcl-2,caspase-3,and interleukin-8 expression,but not on NF-κB and the heat shock proteins.Taken together,argon preconditioning protects against apoptosis of neuronal cells and mediates its action via Toll-like receptors.Argon may represent a promising therapeutic alternative in various clinical settings,such as the treatment of stroke. 展开更多
关键词 apoptosis inflammation interleukin-8 neuroprotection ROTENONE SH-SY5Y Toll-like receptor 2 Toll-like receptor 4
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Fingolimod protects against neurovascular unit injury in a rat model of focal cerebral ischemia/reperfusion injury 被引量:3
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作者 Xiao-Yu Zhu Ting-Ting Ma +4 位作者 Yang Li Ming-Qi Zhang Liang Zhao Jia Liang Lian-Qiu Min 《Neural Regeneration Research》 SCIE CAS CSCD 2023年第4期869-874,共6页
Recent research on the underlying mechanisms of cerebral ischemia indicates that the neurovascular unit can be used as a novel subject for general surveys of neuronal damage and protein mechanisms.Fingolimod(FTY-720)i... Recent research on the underlying mechanisms of cerebral ischemia indicates that the neurovascular unit can be used as a novel subject for general surveys of neuronal damage and protein mechanisms.Fingolimod(FTY-720)is a newly developed immunosuppressant isolated from Cordyceps sinensis that exhibits a wide range of biological activities,and has recently attracted much attention for the treatment of ischemic cerebrovascular diseases.In the current research,the role of FTY-720 and its possible mechanisms were assessed from an neurovascular unit perspective using a rat cerebral ischemia model.Our results revealed that FTY-720 markedly decreased infarct volume,promoted neurological function recovery,and weakened the blood-brain barrier permeability of ischemic rats.The protective roles of FTY-720 in ischemic stroke are ascribed to a combination of sphingosin-1-phosphate receptor-1 and reduced expression of sphingosin-1-phosphate receptor-1 in microvessels and reduction of interleukin-17A protein levels.These findings indicate that FTY-720 has promise as a new therapy for neurovascular protection and functional recovery after ischemic stroke. 展开更多
关键词 astrocyte blood-brain barrier CLAUDIN-5 FTY-720 interleukin-17A ischemic stroke neural protection neurovascular unit OCCLUDIN sphingosine-1-phosphate receptor 1
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Mepolizumab induced palmoplantar psoriasis:A case report
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作者 Fabio Artosi Laura Diluvio +2 位作者 Martina Vultaggio Elena Campione Luca Bianchi 《World Journal of Clinical Cases》 SCIE 2023年第26期6154-6158,共5页
BACKGROUND Atopic dermatitis and asthma are two diseases whose pathogenesis is largely attributable to the activation,at least in the initial stages,of T helper(Th)-2 Lymphocytes,the related cytokine axis,and B lympho... BACKGROUND Atopic dermatitis and asthma are two diseases whose pathogenesis is largely attributable to the activation,at least in the initial stages,of T helper(Th)-2 Lymphocytes,the related cytokine axis,and B lymphocytes with antibody production.Psoriasis is conversely a pathology resulting from a recruitment of Th-17 and Th-1 lymphocytes,after an initial role of innate immunity.Mepolizumab is a humanized monoclonal antibody directed against interleukin(IL)-5,a central cytokine in the Th-2 axis,therefore involved in the pathogenesis of asthma.Several authors have described the appearance of psoriatic lesions in patients with asthma or atopic dermatitis following the therapy with dupilumab,a monoclonal antibody that blocks the interleukin(IL)-4,another Th-2 cytokine.CASE SUMMARY We present the case of a 59-year-old patient who developed psoriasiform lesions on the palms after mepolizumab therapy for asthma,for the activation of the parallel cytokine cascade after the blockade of IL-5.We successfully treated the patient with a topical calcipotriol and betamethasone ointment.CONCLUSION We should investigate with further attention the possible impact on the human immunological ecosystem put in place by the inhibition of the activity of individual inflammatory mediators,so as to be able to recognize the initial adverse effects early. 展开更多
关键词 PSORIASIS interleukin-5 MEPOLIZUMAB ASTHMA IMMUNOLOGY Case report
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计算光度法同时测定人发中的锌铜铁 被引量:10
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作者 王咏梅 林朝阳 +2 位作者 李克安 魏永巨 童沈阳 《分析试验室》 CAS CSCD 北大核心 1995年第3期18-20,共3页
本文以5-溴-(二乙基氨基)-2-(2-吡啶偶氨)苯酚为显色剂,用多元线性回归或卡尔曼滤波法处理多波长测量数据,在pH5.11的30%乙醇介质中同时测定了人发中的锌、铜和铁。
关键词 人发 5-BR-PADAP 计算光度法
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