Analysis of the Role of D-Dimer,Interleukin-6,and Interleukin-18 in Differential Diagnosis of Pediatric Refractory Mycoplasma pneumoniae Pneumonia

Objective:To analyze the value of D-dimer(D-D),interleukin-6(IL-6),and IL-18 in the differential diagnosis of children with refractory Mycoplasma pneumoniae pneumonia(RMPP).Methods:The medical records of 92 children with Mycoplasma pneumoniae pneumonia(MPP)treated in the hospital were selected for retrospective analysis from January 2023 to January 2024.After comprehensive examinations such as computed tomography examination of the chest,48 children with general Mycoplasma pneumoniae pneumonia(GMPP)were put in the GMPP group and 44 children with RMPP were grouped in the RMPP group.The IL-6,IL-18,and D-D levels were compared between the two groups,and the receiver operating characteristic(ROC)curves were plotted to analyze their value for differential diagnosis of RMPP.Results:The levels of IL-6,IL-18,and D-D in the RMPP group were higher than those in the GMPP group(P<0.05);the ROC curves showed that the specificity of the differential diagnosis of IL-6,IL-18,and D-D was higher,and their diagnostic value was significant.Conclusion:Determination of IL-6,IL-18,and D-D levels in children with MPP can further diagnose the children’s condition,which can help physicians formulate targeted treatment plans,and is of great significance to the improvement of the children’s condition,which is worthy of attention.

Interleukin-6 in epilepsy and its neuropsychiatric comorbidities: How to bridge the gap

There is growing evidence that interleukin(IL)-6 plays an important role in neurological and psychiatric disorders.This editorial comments on the study published in the recent issue of the World Journal of Psychiatry,which employed Mendelian randomization to identify a causal relationship between IL-6 receptor blockade and decreased epilepsy incidence.The purpose of this editorial is to highlight the dual effects of IL-6 in epilepsy and its related neuropsychiatric comorbidities.IL-6 plays a critical role in the facilitation of epileptogenesis and maintenance of epileptic seizures and is implicated in neuroinflammatory proce-sses associated with epilepsy.Furthermore,IL-6 significantly influences mood regulation and cognitive dysfunction in patients with epilepsy,highlighting its involvement in neuropsychiatric comorbidities.In summary,IL-6 is not only a pivotal factor in the pathogenesis of epilepsy but also significantly contributes to the emergence of epilepsy-related neuropsychiatric complications.Future resear-ch should prioritize elucidating the specific mechanisms by which IL-6 operates across different subtypes,stages and neuropsychiatric comorbidities of epilepsy,with the aim of developing more precise and effective interventions.Furthermore,the potential of IL-6 as a biomarker for the early diagnosis and prognosis of epile-psy warrants further investigation.

Interleukin-17A facilitates tumor progression via upregulating programmed death ligand-1 expression in hepatocellular carcinoma

BACKGROUND Hepatocellular carcinoma(HCC)is an inflammation-associated tumor with a dismal prognosis.Immunotherapy has become an important treatment strategy for HCC,as immunity is closely related to inflammation in the tumor microenvir-onment.Inflammation regulates the expression of programmed death ligand-1(PD-L1)in the immunosuppressive tumor microenvironment and affects im-munotherapy efficacy.Interleukin-17A(IL-17A)is involved in the remodeling of the tumor microenvironment and plays a protumor or antitumor role in different tumors.We hypothesized that IL-17A participates in tumor progression by affe-cting the level of immune checkpoint molecules in HCC.The upregulation of PD-L1 expression in HCC cells by IL-17A was assessed by reverse transcription PCR,western blotting,and flow cytometry.Mechanistic studies were conducted with gene knockout models and pathway inhibitors.The function of IL-17A in immune evasion was explored through coculture of T cells and HCC cells.The effects of IL-17A on the malignant biological behaviors of HCC cells were evaluated in vitro,and the antitumor effects of an IL-17A inhibitor and its synergistic effects with a PD-L1 inhibitor were studied in vivo.RESULTS IL-17A upregulated PD-L1 expression in HCC cells in a dose-dependent manner,whereas IL-17A receptor knockout or treatment with a small mothers against decapentaplegic 2 inhibitor diminished the PD-L1 expression induced by IL-17A.IL-17A enhanced the survival of HCC cells in the coculture system.IL-17A increased the viability,G2/M ratio,and migration of HCC cells and decreased the apoptotic index.Cyclin D1,VEGF,MMP9,and Bcl-1 expression increased after IL-17A treatment,whereas BAX expression decreased.The combination of IL-17A and PD-L1 inhibitors showed synergistic antitumor efficacy and increased cluster of differentiation 8+T lymphocyte infiltration in an HCC mouse model.CONCLUSION IL-17A upregulates PD-L1 expression via the IL-17A receptor/phosphorylation-small mothers against decapenta-plegic 2 signaling pathway in HCC cells.Blocking IL-17A enhances the therapeutic efficacy of PD-L1 antibodies in HCC in vivo.

磁刺激下Fe_(3)O_(4)@ZIF-8纳米颗粒影响骨髓间充质干细胞的成骨分化

背景:骨髓间充质干细胞在组织工程和骨再生中具有重要作用,然而如何促进骨髓间充质干细胞的成骨分化仍然是一个挑战。目的:探讨磁刺激下Fe_(3)O_(4)@ZIF-8纳米颗粒对骨髓间充质干细胞成骨分化的影响。方法:采用水热法合成沸石咪唑酯骨架(ZIF-8),采用一锅法合成具有磁性的Fe_(3)O_(4)@ZIF-8纳米颗粒(材料制备中分别加入2.5,5,10,20μg的Fe_(3)O_(4)),通过扫描电镜、X射线光电子能谱、X射线衍射、振动样品磁强计检测等对Fe_(3)O_(4)@ZIF-8纳米颗粒进行表征,筛选出合适的材料进行后续实验。提取4周龄SD大鼠骨髓间充质干细胞,分别与不同质量浓度(25,50,75,100,125μg/mL)的Fe_(3)O_(4)@ZIF-8纳米颗粒溶液共培养,CCK-8法检测细胞增殖,筛选出最佳的材料溶液质量浓度;筛选出材料溶液质量浓度后,施加磁刺激(磁场强度分别为0,50,100,150 MT),CCK-8法检测细胞增殖,筛选出最佳的磁场强度与Fe_(3)O_(4)@ZIF-8纳米颗粒,用于骨髓间充质干细胞诱导分化实验。将SD大鼠骨髓间充质干细胞分别与ZIF-8、Fe_(3)O_(4)@ZIF-8、Fe_(3)O_(4)@ZIF-8(磁场干预)纳米颗粒溶液共培养,以单独培养的细胞为空白对照,成脂诱导后进行油红O染色,成骨诱导后进行碱性磷酸酶、茜素红染色与Runx2蛋白质量浓度检测。结果与结论:(1)扫描电镜下可见Fe_(3)O_(4)@ZIF-8纳米颗粒呈现十二面体结构,随着材料中Fe_(3)O_(4)含量的增加,纳米颗粒的粒径增大,选择粒径约250 nm(该粒径下的纳米颗粒功能性及生物安全性较稳定)的Fe_(3)O_(4)@ZIF-8纳米颗粒(材料制备中分别加入5,10μg的Fe_(3)O_(4))进行后续实验。(2)CCK-8检测结果显示,在100MT磁场作用下,50μg/mL的Fe_(3)O_(4)@ZIF-8纳米颗粒(材料制备中加入10μg的Fe_(3)O_(4))能够显著促进骨髓间充质干细胞的增殖,选择该条件下的纳米颗粒进行骨髓间充质干细胞成骨诱导分化实验。(3)成骨诱导后,Fe_(3)O_(4)@ZIF-8(磁场干预)组骨髓间充质干细胞的碱性磷酸酶活性、细胞外基质矿化程度与Runx2蛋白质量浓度均高于其他3组(P<0.05);成脂诱导后,Fe_(3)O_(4)@ZIF-8(磁场干预)组骨髓间充质干细胞的脂滴形成少于其他3组(P<0.05)。(4)结果表明,在特定的磁场条件下Fe_(3)O_(4)@ZIF-8纳米颗粒可以促进骨髓间充质干细胞的成骨分化。

C-X-C chemokine receptor type 5+CD8+T cells as immune regulators in hepatitis Be antigen-positive chronic hepatitis B under interferonalpha treatment

BACKGROUND C-X-C chemokine receptor type 5(CXCR5)+CD8+T cells represent a unique immune subset with dual roles,functioning as cytotoxic cells in persistent viral infections while promoting B cell responses.Despite their importance,the specific role of CXCR5+CD8+T cells in chronic hepatitis B(CHB),particularly during interferon-alpha(IFN-α)treatment,is not fully understood.This study aims to elucidate the relationship between CXCR5+CD8+T cells and sustained serologic response(SR)in patients undergoing 48 weeks of pegylated IFN-α(peg-IFN-α)treatment for CHB.AIM To elucidate the relationship between CXCR5+CD8+T cells and sustained SR in patients undergoing 48 weeks of peg-IFN-αtreatment for CHB.METHODS This study enrolled 60 patients with hepatitis Be antigen(HBeAg)-positive CHB undergoing 48 weeks of peg-IFN-αtreatment.Participants were assessed for eligibility based on criteria such as persistent HBsAg-positive status for at least six months,HBeAb-negative,hepatitis B virus DNA levels exceeding 2×104 copies/mL,and alanine aminotransferase(ALT)levels between 2 and 10 times the upper limit of normal.Blood samples were collected at baseline and at weeks 12,24,48,and a 24-week treatment-free follow-up(week 72)to measure serum interleukin(IL)-21 concentration via ELISA and to analyze CXCR5 and programmed death-ligand 1(PD-L1)expression on CD8+T cells by flow cytometry,CXCR5 is a chemokine receptor that directs immune cells to specific tissues,while PD-L1 is a protein that regulates immune responses by inhibiting T cell activity.RESULTS Patients with CHB exhibited significantly lower levels of circulating CXCR5+CD8+T cells compared to healthy controls(P<0.01).Notably,CXCR5+CD8+T cells were prominently expressed in patients who achieved sustained SR compared to non-SR(NSR).A significant correlation was observed between CXCR5 and PD-L1 expression(r=-0.189,P=0.002).However,there was no significant correlation between serum IL-21 levels and CXCR5+CD8+lymphocytes(r=-0.03,P=0.625)or serum ALT levels(r=0.026,P=0.678).CONCLUSION The enhanced expression of CXCR5+CD8+T cells in patients achieving HBeAg seroconversion during IFN-αtreatment suggests that these cells play a crucial role in antiviral immune responses against hepatitis B.This study highlights the potential of CXCR5+CD8+T cells as immune regulators in CHB,which may inform future therapeutic strategies to optimize antiviral treatments.

Association of interleukin-6 with acute lung injury risk and disease severity in sepsis

Sepsis is a life-threatening condition caused by a dysregulated response of the body in response to an infection that harms its tissues and organs.Interleukin-6(IL-6)is a significant component of the inflammatory response as part of the pa-thogenesis of sepsis.It aids in the development of Acute lung injury and,subse-quently,multiple organ dysfunction syndrome.This letter probes into the corre-lation between plasma IL-6 levels and the risk of developing acute lung injury and multiple organ dysfunction syndrome in critically ill patients with sepsis.While it shows promising results,limitations like its observational study design,a limited sample size,a single center involvement,single-time-point measurement,and a lack of a control group restrain its cogency.The study is a big step in identifying IL-6 as a biomarker to improve patient care.

基于改进YOLOv8的红外船舶检测

针对现有红外船舶检测算法检测精度低和实时性不足问题,提出一种基于改进YOLOv8的红外船舶检测算法。首先,将设计的MCA机制引入到YOLOv8的主干网络,增强主干网络的多尺度特征提取能力;其次,对YOLOv8的检测头进行共享参数和重参数化设计,以此提升检测头的检测效率;然后,使用BiFPN结构改进YOLOv8的颈部网络,利用双向信息流和可学习权重加强网络的特征表达能力;最后,使用Faster Block对YOLOv8的C2f模块进行改进,保持精度的同时减少参数量,提升算法的检测速度。该算法在红外船舶数据集上测试,mAP值达到了93.1%,相比较原算法提高了2.5个百分点,参数量比原算法减少了32.6%。实验结果表明,改进后的算法比原算法有了较大提升,证明了改进算法的有效性。

Small heat shock protein B8:from cell functions to its involvement in diseases and potential therapeutic applications

Heat shock protein family B(small)member 8(HSPB8)is a 22 kDa ubiquitously expressed protein belonging to the family of small heat shock proteins.HSPB8 is involved in various cellular mechanisms mainly related to proteotoxic stress response and in other processes such as inflammation,cell division,and migration.HSPB8 binds misfolded clients to prevent their aggregation by assisting protein refolding or degradation through chaperone-assisted selective autophagy.In line with this function,the pro-degradative activity of HSPB8 has been found protective in several neurodegenerative and neuromuscular diseases characterized by protein misfolding and aggregation.In cancer,HSPB8 has a dual role being capable of exerting either a pro-or an anti-tumoral activity depending on the pathways and factors expressed by the model of cancer under investigation.Moreover,HSPB8 exerts a protective function in different diseases by modulating the inflammatory response,which characterizes not only neurodegenerative diseases,but also other chronic or acute conditions affecting the nervous system,such as multiple sclerosis and intracerebellar hemorrhage.Of note,HSPB8 modulation may represent a therapeutic approach in other neurological conditions that develop as a secondary consequence of other diseases.This is the case of cognitive impairment related to diabetes mellitus,in which HSPB8 exerts a protective activity by assuring mitochondrial homeostasis.This review aims to summarize the diverse and multiple functions of HSPB8 in different pathological conditions,focusing on the beneficial effects of its modulation.Drug-based and alternative therapeutic approaches targeting HSPB8 and its regulated pathways will be discussed,emphasizing how new strategies for cell and tissue-specific delivery represent an avenue to advance in disease treatments.

改进YOLOv8n的无人机航拍图像检测算法

针对无人机航拍图像中目标小、尺度变化大和背景干扰等因素导致检测精度低、定位不准确的问题,提出一种改进YOLOv8n的无人机航拍图像目标检测算法。首先改进C2f模块,利用可变形卷积(DCN)替换其Bottleneck中的卷积以适应航拍图像中物体的形变和尺度变化,同时,在主干网络引入LSK注意力机制,实现动态调整空间感受野,从而在特征提取阶段更灵活地适应不同目标对背景信息需求的差异;然后改进颈部网络,增加一个较浅的检测层并移除大目标检测层,使网络能更有效地捕获小目标的特征以提升检测精度;最后引入WIoU损失函数,使模型更加关注低质量样本,得到更高的检测精度。在VisDrone2019数据集上进行对比实验和消融实验,mAP_(50)值较基线算法模型提升了5.2个百分点,参数量减少了20%,检测速度(FPS)达到87帧/s,能够满足实时性的检测需求。与主流算法进行对比实验,所提算法表现优于目前的主流算法。在DOTA数据集上进行泛化实验,mAP_(50)值提升了1.7个百分点,证明所提算法具有通用性。

融合注意力机制的YOLOv8-TS交通标志检测网络

道路交通标志识别是自动驾驶、车联网的重要组成部分,为进一步提高交通标志检测的精度和速度,提出一种基于YOLOv8s改进的YOLOv8-TS道路交通标志检测网络。首先,对YOLOv8s进行了整体的轻量化设计,并设计了Conv-G7S和CSP-G7S模块,减少了网络的参数量;其次,设计了CSP-SwinTransformer模块,强化了模型利用窗口内的特征信息进行上下文感知和建模的能力;然后,在颈部网络融合了卷积注意力机制(CBAM),强化了模型对不同通道、空间权重信息的学习;最后,对损失函数进行了改进,提升了边界框回归性能。实验结果表明,在中国道路交通标志TT100K数据集上,精确率(Precision)、平均精度(mAP@0.5)分别提高了6.9%、3.7%,而改进后模型的参数量下降了75.4%,模型的大小仅为5.8 MB,平均精度(mAP@0.5)达到96.5%,检测速度由126.58 f/s提升至136.99 f/s。

Expression change of interleukin-8 gene in rabbit basilar artery after subarachnoid hemorrhage

Objective To study the expression change of interleukin-8 （IL-8） gene in the basilar artery of rabbit and the effect of IL-8 on the development of cerebral vasospasm induced by experimental subarachnoid hemorrhage （SAH）. Methods Thirty five healthy Japanese White Rabbits were randomly divided into saline-control group and experimental group. The experimental group was subdivided into four groups, representing day 1, 4, 7 and 14 after the first blood injection of SAH. The delayed cerebral vasospasm （DCVS） model was established by double injection of autologous blood into the cisterna magna. The expression change of cytokine IL-8 mRNA in the basilar artery was analyzed by RT- PCR. Results The expression of IL-8 gene increased on day 4-7 after the first blood injection of SAH compared with control （P 〈 0.001）, and decreased to normal on day 14. The expression of IL-8 gene in the SAH groups were positively correlated with the degree of basilar artery stenosis （r = 0.642, P 〈 0.01）. Conclusion The expression level of IL-8 gene in basilar arteries was intimately associated with the degree of cerebral vasospasm, suggesting that IL-8 may play an important role in the DCVS after SAH as an immunological inflammatory factor.

血清中Interleukin-18含量的检测对结肠癌预后判断的价值

目的 探讨结肠癌患者血清中白细胞介素 18(IL 18)水平与预后的关系。方法 收集 10 6例结肠癌患者和 60例健康成人的血清 ,采用酶联免疫吸附反应 (ELISA )检测IL 18的水平。结果 1997年 6月及以前的血清 5 9份 ,IL 18的平均含量为3 3 8.46pg/ml ;1997年 6月以后的血清 47份 ,IL 18的平均含量为 3 2 8.85 pg/ml ;2组比较无显著性差异 (P <0 .0 5 ) ;结肠癌组患者血清IL 18的含量 ( 3 46.5 3 pg/ml± 3 1.2 3pg/ml)明显高于正常对照组 ( 2 3 3 .3 2 pg/ml± 2 2 .5 4pg/ml) ,有显著性差异 (P <0 .0 5 ) ;Ⅱ期、Ⅲ期和Ⅳ期患者血清中IL 18水平明显高于对照组 (P <0 .0 5 ) ,血清IL 18≥ 3 46pg/ml组的患者 5年生存率为 5 .3 % ,血清IL 18<3 46pg/ml的患者 5年生存率为 18.6% ;两组比较有显著性差异 (P <0 .0 5 ) ;多因素分析表明 ,血清IL 18含量是判断结肠癌患者预后的独立因素。结论 血清中IL 18含量与结肠癌患者 5年生存率有密切相关。血清中IL

1,8-桉叶油素干预大鼠实验性牙周炎模型的炎症反应

背景:研究表明,天然植物精油提取物1,8-桉叶油素具有抗炎、抗氧化、抗菌、抗肿瘤等多种药理作用,在多种疾病中表现出抗炎作用。目的:探讨1,8-桉叶油素对大鼠实验性牙周炎模型炎症反应的影响。方法:按照完全随机数字表法将30只SD大鼠随机分为正常对照组、牙周炎对照组和1,8-桉叶油素组,每组10只。牙周炎对照组和1,8-桉叶油素组利用正畸钢丝结扎法构建实验性牙周炎模型。造模8周后,正常对照组和牙周炎对照组大鼠颊、腭侧牙周袋内均注射生理盐水,1,8-桉叶油素组大鼠颊、腭侧牙周袋内注射1,8-桉叶油素溶液,2次/d,连续给药4周。给药结束后,进行牙周临床指标检测、牙周组织学评估、血清炎症因子水平检测及牙龈组织中炎症因子m RNA与蛋白表达检测。结果与结论:①与正常对照组比较,牙周炎对照组大鼠牙龈出血指数、牙周探诊深度增加(P<0.05),血清中白细胞介素1β、肿瘤坏死因子α、白细胞介素6水平升高(P<0.05),血清中白细胞介素10水平降低(P<0.05),牙龈组织中白细胞介素1β、肿瘤坏死因子α、白细胞介素6mRNA与蛋白表达均升高(P<0.05),牙龈组织中白细胞介素10 mRNA与蛋白表达均降低(P<0.05);苏木精-伊红染色结果显示,牙周炎对照组大鼠牙周组织炎症明显。②与牙周炎对照组比较,1,8-桉叶油素组大鼠牙龈出血指数、牙周探诊深度减少(P<0.05),血清中白细胞介素1β、肿瘤坏死因子α、白细胞介素6水平降低(P<0.05),血清中白细胞介素10水平升高(P<0.05),牙龈组织中白细胞介素1β、肿瘤坏死因子α、白细胞介素6 mRNA与蛋白表达均降低(P<0.05),牙龈组织中白细胞介素10 mRNA与蛋白表达均升高(P<0.05);苏木精-伊红染色结果显示,1,8-桉叶油素组大鼠牙周组织炎症明显减轻。结果表明,1,8-桉叶油素治疗可减轻大鼠实验性牙周炎模型的炎症反应。

白细胞介素-8与类风湿关节炎关联性研究进展

类风湿关节炎(rheumatoid arthritis,RA)是一种多关节慢性炎症性自身免疫性疾病,其发病机制主要包括炎症反应、软骨破坏及骨侵蚀和血管生成,并与炎症因子密切相关。其中白细胞介素-8(IL-8)为炎症因子中重要一员,故本文从炎症、软骨破坏及骨侵蚀和血管生成三方面对IL-8在RA发生发展中的作用机制展开综述。本文通过阐述IL-8与RA的相关性,明确IL-8在RA发病机制中的作用,以期为今后深入研究RA提供新的参考依据。

ZIF-8@TiO_(2)复合材料的制备及光催化性能

本研究采用水热法制备了一种新型的高效光催化微米复合材料ZIF-8@TiO_(2),并比较了ZIF-8、TiO_(2)纳米球和ZIF-8@TiO_(2)光催化降解罗丹明B的能力。通过扫描电子显微镜(SEM)、X射线衍射(XRD)仪、X射线光电子能谱(XPS)仪、紫外-可见漫反射光谱(DRS)仪、傅里叶变换红外光谱(FTIR)仪和比表面积测量(BET)仪对合成的材料进行了表征。结果表明,TiO_(2)纳米球在ZIF-8表面均匀生长,ZIF-8和TiO_(2)通过化学键连接在一起。ZIF-8和TiO_(2)的协同作用大大增强了复合光催化剂的催化能力。其中ZIF-8@TiO_(2)复合材料的ZIF-8为电荷分离器中光生电子转移提供了一种新的方式,以降低电荷复合率。由于该混合物结合了ZIF-8和TiO_(2)的优点,ZIF-8@TiO_(2)显示出优异的光催化降解罗丹明B的能力。同时讨论了复合光催化剂可能的光催化机理。

聚乙烯醇/ZIF-8复合膜的制备及吸附性能

通过溶液流延法制备了聚乙烯醇/ZIF-8复合膜,采用SEM、FT-IR、XRD、TGA、BET等对聚乙烯醇/ZIF-8复合膜进行表征,并探究了复合膜的水稳定性、机械性能,以及对刚果红(CR)的吸附性能。结果表明:聚乙烯醇与ZIF-8材料复合后能改善聚乙烯醇的热稳定性,增加膜表面粗糙度;吸附过程以化学吸附为主,通过内部扩散控制吸附速率,符合准二级吸附动力学模型和Langmuir吸附曲线;ZIF-8质量分数为10%时制备的聚乙烯醇/ZIF-8复合膜,在温度20℃、刚果红溶液质量浓度95 mg/L时,对刚果红的最大吸附量达159.63 mg/g,使用4次后仍能保持93%的吸附效率。

基于改进YOLOv8的嵌入式道路裂缝检测算法

在边缘端设备部署YOLOv8L模型进行道路裂缝检测可以实现较高的精度,但难以保证实时检测。针对此问题,提出一种可部署到边缘计算设备Jetson AGX Xavier上的基于改进YOLOv8模型的目标检测算法。首先,利用部分卷积设计Faster Block结构以替换YOLOv8 C2f模块中的Bottleneck结构,并将改进后的C2f模块记为C2f-Faster;其次,在YOLOv8主干网络中的每个C2f-Faster模块之后接一个SE(Squeeze-and-Excitation)通道注意力层,进一步提高检测的精度。在开源道路损害数据集RDD20(Road Damage Detection 20)上的实验结果表明:所提方法的平均F1得分为0.573,每秒检测帧数(FPS)为47,模型大小为55.5MB,相较于GRDDC2020(GlobalRoadDamageDetection Challenge 2020)的SOTA(State-Of-The-Art)模型,F1得分提高了0.8个百分点,FPS提高了291.7%,模型大小减小了41.8%,实现了在边缘设备上对道路裂缝实时且准确的检测。

支气管镜介入治疗对RMPP患儿的疗效及其对外周血CRP、SF、IL-8水平及CD4^(+)/CD8^(+)比值的影响

目的探讨支气管镜介入治疗对难治性肺炎支原体肺炎(RMPP)患儿的疗效及其对外周血C反应蛋白(CRP)、铁蛋白(SF)、白细胞介素(IL)-8水平及CD4^(+)/CD8^(+)比值的影响。方法选取2020年12月至2022年12月河北省儿童医院收治的195例确诊为RMPP患儿作为研究对象,采用随机数字表法分为对照组和介入组(病程≥14 d归为晚期介入组、病程<14 d归为早期介入组)。根据临床疗效将130例接受支气管镜介入治疗的患儿分为有效组和无效组。对照组进行常规治疗,晚期介入组和早期介入组在对照组的基础上实施支气管镜介入治疗。比较对照组、晚期介入组和早期介入组临床疗效、临床症状与体征改善情况;检测并比较对照组、晚期介入组和早期介入组外周血CRP、SF、IL-8水平及CD4^(+)/CD8^(+)比值;比较对照组、晚期介入组和早期介入组不良反应发生率;比较有效组和无效组外周血CRP、SF、IL-8水平及CD4^(+)/CD8^(+)比值。采用受试者工作特征(ROC)曲线分析外周血CRP、SF、IL-8、CD4^(+)/CD8^(+)比值对支气管镜介入治疗RMPP疗效的预测价值。结果早期介入组总有效率高于对照组和晚期介入组,差异均有统计学意义(P<0.05);对照组和晚期介入组总有效率比较,差异无统计学意义(P>0.05)。早期介入组咳嗽持续时间、发热持续时间、住院时间均短于对照组和晚期介入组,差异均有统计学意义(P<0.05)。对照组、晚期介入组和早期介入组治疗后外周血CD4^(+)/CD8^(+)比值均高于治疗前,CRP、SF、IL-8水平均低于治疗前,差异均有统计学意义(P<0.05);早期介入组治疗后外周血CD4^(+)/CD8^(+)比值均高于对照组和晚期介入组,CRP、SF、IL-8水平均低于对照组和晚期介入组,差异均有统计学意义(P<0.05)。早期介入组出现不良反应8例,晚期介入组出现不良反应10例,均暂停灌洗操作后很快恢复。对照组未出现上述不良反应。无效组外周血CRP、SF、IL-8水平均高于有效组,CD4^(+)/CD8^(+)比值低于有效组,差异均有统计学意义(P<0.05)。ROC曲线分析结果显示,CRP、SF、IL-8、CD4^(+)/CD8^(+)比值联合检测预测支气管镜介入治疗RMPP无效的曲线下面积、灵敏度、特异度分别为0.871、81.81%、83.80%。结论早期应用支气管镜介入治疗对RMPP的疗效较好,可改善患儿外周血CRP、SF、IL-8水平及CD4^(+)/CD8^(+)比值,且外周血CRP、SF、IL-8、CD4^(+)/CD8^(+)比值联合检测有助于预测支气管镜介入治疗RMPP的疗效。

miR-567通过调控CDK8在NSCLC增殖、迁移和细胞周期中的作用及其临床相关性研究

目的探讨微小RNA(miR)-567通过调控周期蛋白依赖性激酶8(CDK8)在非小细胞肺癌(NSCLC)增殖、迁移和细胞周期中的作用及其临床相关性研究。方法收集40例NSCLC患者的肿瘤组织和临近癌旁组织,采用实时荧光定量PCR(qRT-PCR)检测miR-567和CDK8的表达。将miR-NC mimic、miR-567 mimic、oe-NC和oe-CDK8转染至A549和H1975细胞中,使用qRT-PCR检测miR-567和CDK8的表达,CCK-8法检测细胞增殖水平,Transwell法检测细胞迁移水平,流式细胞术检测细胞周期变化。通过荧光素酶报告基因实验检测miR-567与CDK8的靶向性。结果在NSCLC患者的肿瘤组织中,miR-567表达降低,而CDK8表达升高,二者呈负相关(P<0.05)。在A549和H1975细胞中,miR-567 mimic组相较于miR-NC mimic组,miR-567表达升高,CDK8表达降低,细胞增殖和迁移水平降低,细胞G1期比例升高,S期比例降低;miR-567 mimic组在正常型CDK8中,荧光强度低于miR-NC mimic组;miR-567 mimic+oe-CDK8组相较于miR-567 mimic+oe-NC组,CDK8表达升高,细胞增殖和迁移水平升高,细胞G1期比例降低,S期比例升高。结论miR-567通过靶向抑制CDK8表达,控制肿瘤细胞在S期阻滞,从而抑制NSCLC的增殖和迁移能力。

鄂尔多斯盆地二叠系盒8段河流扇沉积模式及勘探意义

根据野外露头及岩心的分析化验资料,在源-汇系统分析的基础上,对鄂尔多斯盆地二叠系盒8段的古气候环境及沉积相特征进行了详细研究。研究结果表明:(1)鄂尔多斯盆地二叠系盒8段Sr/Cu的值多大于10,V/Cr值多小于2.0,地层厚度差小于30 m,未出现明显的突变,古沉积坡度为0.5°~1.0°,古地貌开阔平缓且无明显坡折带,沉积水体较浅,是在古气候干旱、古地形平缓的背景下,受阶段性洪水作用主导的事件沉积。(2)盒8段沉积期,盆地不存在大面积汇水区,沉积体系中河流并未进入湖泊水体,而是向东南流出盆地或是消失在内陆干旱地区,全盆地以洪泛平原沉积为主,表现为河道与泛滥平原交互分布的沉积格局,形成了河流扇沉积模式。(3)湖水进退往复,洪水期与枯水期间歇发育,洪水期多期次决口河道接力搬运使得粗粒级砂岩向湖盆长距离延伸,枯水期发育厚层泥质沉积可作为区域性盖层,形成了良好的储盖组合,勘探潜力大。