Background:Erzhu Erchen decoction(EZECD),which is based on Erchen decoction and enhanced with Atractylodes lancea and Atractylodes macrocephala,is widely used for the treatment of dampness and heat(The clinical manife...Background:Erzhu Erchen decoction(EZECD),which is based on Erchen decoction and enhanced with Atractylodes lancea and Atractylodes macrocephala,is widely used for the treatment of dampness and heat(The clinical manifestations of Western medicine include thirst,inability to drink more,diarrhea,yellow urine,red tongue,et al.)internalized disease.Nevertheless,the mechanism of EZECD on damp-heat internalized Type 2 diabetes(T2D)remains unknown.We employed data mining,pharmacology databases and experimental verification to study how EZECD treats damp-heat internalized T2D.Methods:The main compounds or genes of EZECD and damp-heat internalized T2D were obtained from the pharmacology databases.Succeeding,the overlapped targets of EZECD and damp-heat internalized T2D were performed by the Gene Ontology,kyoto encyclopedia of genes and genomes analysis.And the compound-disease targets-pathway network were constructed to obtain the hub compound.Moreover,the hub genes and core related pathways were mined with weighted gene co-expression network analysis based on Gene Expression Omnibus database,the capability of hub compound and genes was valid in AutoDock 1.5.7.Furthermore,and violin plot and gene set enrichment analysis were performed to explore the role of hub genes in damp-heat internalized T2D.Finally,the interactions of hub compound and genes were explored using Comparative Toxicogenomics Database and quantitative polymerase chain reaction.Results:First,herb-compounds-genes-disease network illustrated that the hub compound of EZECD for damp-heat internalized T2D could be quercetin.Consistently,the hub genes were CASP8,CCL2,and AHR according to weighted gene co-expression network analysis.Molecular docking showed that quercetin could bind with the hub genes.Further,gene set enrichment analysis and Gene Ontology represented that CASP8,or CCL2,is negatively involved in insulin secretion response to the TNF or lipopolysaccharide process,and AHR or CCL2 positively regulated lipid and atherosclerosis,and/or including NOD-like receptor signaling pathway,and TNF signaling pathway.Ultimately,the quantitative polymerase chain reaction and western blotting analysis showed that quercetin could down-regulated the mRNA and protein experssion of CASP8,CCL2,and AHR.It was consistent with the results in Comparative Toxicogenomics Database databases.Conclusion:These results demonstrated quercetin could inhibit the expression of CASP8,CCL2,AHR in damp-heat internalized T2D,which improves insulin secretion and inhibits lipid and atherosclerosis,as well as/or including NOD-like receptor signaling pathway,and TNF signaling pathway,suggesting that EZECD may be more effective to treat damp-heat internalized T2D.展开更多
Central venous catheterization was a common technology in clinical anesthesia and rescue. Guide wire fracture and retention was a very rare and severe complication in central venous catheterization. Here, we reported...Central venous catheterization was a common technology in clinical anesthesia and rescue. Guide wire fracture and retention was a very rare and severe complication in central venous catheterization. Here, we reported a case that guide wire was broken and remained in the body in internal jugular vein puncture process in an 8-year-old boy.展开更多
Objective:To explore the role of endothelial biomarkers in predicting damp-heat syndrome in diabetic kidney disease(DKD).Methods:A total of 183 patients with DKD were divided into 3 groups:the early DKD group,establis...Objective:To explore the role of endothelial biomarkers in predicting damp-heat syndrome in diabetic kidney disease(DKD).Methods:A total of 183 patients with DKD were divided into 3 groups:the early DKD group,established DKD group,and advanced DKD group.All patients were classified according to traditional Chinese medicine(TCM)syndrome type,and clinical indexes were collected for statistical analysis.Results:A total of 183 DKD patients were included in this study.Fibroblast growth factor 23(FGF23),chitinase-3-like protein 1(CHI3L1),endocan,tumor necrosis factor receptor 1(TNFR1),secretory leukocyte protease inhibitor(SLPI),and vascular endothelial growth factor A(VEGF-A)were increased in advanced DKD.FGF23,CHI3L1,endocan,SLPI,and TNFR1 showed a negative correlation with estimated glomerular filtration rate(eGFR),while they had a positive correlation with 24 h urine protein.After adjusting for age,gender,diabetes duration,body mass index(BMI),hemoglobin,glucose,uric acid,24 h urine protein,cholesterol,triglyceride,low-density lipoprotein,and hemoglobin A1c(HbA1c),the multiple regression analysis showed that FGF23,endocan,TNFR1,and SLPI significantly correlated with eGFR.Conclusions:FGF23,endocan,TNFR1,and SLPI are elevated in advanced DKD compared with early stage,and they may take part in the pathogenesis and progression of DKD.Our study provides useful biomarkers for predicting the appearance of damp-heat syndrome,including FGF23,endocan,TNFR1,and SLPI.展开更多
目的:研究清热除湿汤治疗湿热内蕴型面部脂溢性皮炎(SD)患者的疗效及对面部皮肤屏障功能、血清白细胞介素2(IL-2)、白细胞介素6(IL-6)、干扰素-γ(INF-γ)水平的影响。方法:选取2020年5月~2022年5月在本院就诊的82例面部SD(湿热内蕴型)...目的:研究清热除湿汤治疗湿热内蕴型面部脂溢性皮炎(SD)患者的疗效及对面部皮肤屏障功能、血清白细胞介素2(IL-2)、白细胞介素6(IL-6)、干扰素-γ(INF-γ)水平的影响。方法:选取2020年5月~2022年5月在本院就诊的82例面部SD(湿热内蕴型)患者,应用简单随机分组法分为试验组和对照组,各41例。对照组进行西药治疗,试验组此基础上采用清热除湿汤治疗,比较两组患者疗效、中医证候积分、皮肤屏障功能、炎性因子等。结果:试验组治疗总有效率高于对照组( P <0.05);治疗后,红色丘疹、油腻性鳞屑或斑片、瘙痒等主次症及总评分均低于对照组( P <0.05);治疗后,试验组角质层含水量(SCH)、表皮脂质含量、皮肤PH高于对照组,经皮肤水分流失量(TEWL)低于对照组( P <0.05);试验组治疗后IL-2、INF-γ水平高于对照组,IL-6水平低于对照组( P <0.05)。结论清热除湿汤治疗湿热内蕴型面部SD患者,能够提升治疗效果,缓解SD相关症状,改善面部皮肤屏障功能指标和血清炎性因子水平。展开更多
目的调查混合痔外剥内扎术后患者尿潴留(UR)的发生现状及影响因素。方法选择2019年4月—2022年4月医院收治的行外剥内扎术的混合痔手术患者224例为调查对象,收集患者临床资料,采用一般情况调查表、Zung焦虑自评量表(SAS)和视觉模拟评分...目的调查混合痔外剥内扎术后患者尿潴留(UR)的发生现状及影响因素。方法选择2019年4月—2022年4月医院收治的行外剥内扎术的混合痔手术患者224例为调查对象,收集患者临床资料,采用一般情况调查表、Zung焦虑自评量表(SAS)和视觉模拟评分量表(VAS)对患者进行问卷调查,根据患者术后有无UR的发生分为病例组和对照组。采用单因素和多因素Logistic回归分析影响混合痔行外剥内扎术患者术后发生UR的因素。结果发放调查问卷224份,回收有效问卷206份,有效回收率为91.96%,其中病例组74例,对照组132例。单因素分析结果表明,患者性别、年龄、合并糖尿病、合并泌尿系统感染、术前焦虑状况、术后发生便秘、麻醉方式、术后2 h VAS评分、麻醉解除时间、输液量、术后首次排尿时间、手术操作、高密度脂蛋白胆固醇(HDL-C)、血糖(Glu)和白细胞(WBC)水平是影响患混合痔行外剥内扎术患者术后UR发生的因素(P<0.05)。多因素Logistic回归分析结果显示,腰硬联合麻醉(OR=2.247,95%CI=1.257~4.015,P=0.006)、高龄(OR=1.539,95%CI=1.364~1.736,P<0.001)、泌尿系统感染(OR=2.709,95%CI=1.258~5.836,P=0.011)、术前焦虑(OR=2.229,95%CI=1.245~3.990,P=0.007)、术后2 h VAS评分(高)(OR=2.833,95%CI=2.080~3.859,P<0.001)、术后发生便秘(OR=5.104,95%CI=2.733~9.534,P<0.001)、手术操作(不合理)(OR=9.333,95%CI=4.818~18.080,P<0.001)、Glu(高)(OR=4.425,95%CI=2.694~7.268,P<0.001)和HDL-C(低)(OR=0.001,95%CI=0.000~0.008,P<0.001)会导致混合痔行外剥内扎术患者发生UR的风险增高。结论护理人员应高度重视混合痔患者在接受外剥内扎术后出现尿潴留的情况,根据患者的高龄、泌尿系统感染、术前焦虑、腰硬联合麻醉、术后疼痛严重、术后发生便秘、血糖高、高密度脂蛋白胆固醇低等影响因素,制订相应的护理干预措施,精心操作手术,以预防混合痔行外剥内扎术后患者出现尿潴留。展开更多
基金supported by a grant from Hubei Key Laboratory of Diabetes and Angiopathy Program of Hubei University of Science and Technology(2020XZ10)Project of Education Commission of Hubei Province(B2022192).
文摘Background:Erzhu Erchen decoction(EZECD),which is based on Erchen decoction and enhanced with Atractylodes lancea and Atractylodes macrocephala,is widely used for the treatment of dampness and heat(The clinical manifestations of Western medicine include thirst,inability to drink more,diarrhea,yellow urine,red tongue,et al.)internalized disease.Nevertheless,the mechanism of EZECD on damp-heat internalized Type 2 diabetes(T2D)remains unknown.We employed data mining,pharmacology databases and experimental verification to study how EZECD treats damp-heat internalized T2D.Methods:The main compounds or genes of EZECD and damp-heat internalized T2D were obtained from the pharmacology databases.Succeeding,the overlapped targets of EZECD and damp-heat internalized T2D were performed by the Gene Ontology,kyoto encyclopedia of genes and genomes analysis.And the compound-disease targets-pathway network were constructed to obtain the hub compound.Moreover,the hub genes and core related pathways were mined with weighted gene co-expression network analysis based on Gene Expression Omnibus database,the capability of hub compound and genes was valid in AutoDock 1.5.7.Furthermore,and violin plot and gene set enrichment analysis were performed to explore the role of hub genes in damp-heat internalized T2D.Finally,the interactions of hub compound and genes were explored using Comparative Toxicogenomics Database and quantitative polymerase chain reaction.Results:First,herb-compounds-genes-disease network illustrated that the hub compound of EZECD for damp-heat internalized T2D could be quercetin.Consistently,the hub genes were CASP8,CCL2,and AHR according to weighted gene co-expression network analysis.Molecular docking showed that quercetin could bind with the hub genes.Further,gene set enrichment analysis and Gene Ontology represented that CASP8,or CCL2,is negatively involved in insulin secretion response to the TNF or lipopolysaccharide process,and AHR or CCL2 positively regulated lipid and atherosclerosis,and/or including NOD-like receptor signaling pathway,and TNF signaling pathway.Ultimately,the quantitative polymerase chain reaction and western blotting analysis showed that quercetin could down-regulated the mRNA and protein experssion of CASP8,CCL2,and AHR.It was consistent with the results in Comparative Toxicogenomics Database databases.Conclusion:These results demonstrated quercetin could inhibit the expression of CASP8,CCL2,AHR in damp-heat internalized T2D,which improves insulin secretion and inhibits lipid and atherosclerosis,as well as/or including NOD-like receptor signaling pathway,and TNF signaling pathway,suggesting that EZECD may be more effective to treat damp-heat internalized T2D.
文摘Central venous catheterization was a common technology in clinical anesthesia and rescue. Guide wire fracture and retention was a very rare and severe complication in central venous catheterization. Here, we reported a case that guide wire was broken and remained in the body in internal jugular vein puncture process in an 8-year-old boy.
基金This project was supported by the Fundamental Research Funds for the Central Universities(2017-JYB-JS-075)National Key Project for Drug Discovery(2017ZX09304019).
文摘Objective:To explore the role of endothelial biomarkers in predicting damp-heat syndrome in diabetic kidney disease(DKD).Methods:A total of 183 patients with DKD were divided into 3 groups:the early DKD group,established DKD group,and advanced DKD group.All patients were classified according to traditional Chinese medicine(TCM)syndrome type,and clinical indexes were collected for statistical analysis.Results:A total of 183 DKD patients were included in this study.Fibroblast growth factor 23(FGF23),chitinase-3-like protein 1(CHI3L1),endocan,tumor necrosis factor receptor 1(TNFR1),secretory leukocyte protease inhibitor(SLPI),and vascular endothelial growth factor A(VEGF-A)were increased in advanced DKD.FGF23,CHI3L1,endocan,SLPI,and TNFR1 showed a negative correlation with estimated glomerular filtration rate(eGFR),while they had a positive correlation with 24 h urine protein.After adjusting for age,gender,diabetes duration,body mass index(BMI),hemoglobin,glucose,uric acid,24 h urine protein,cholesterol,triglyceride,low-density lipoprotein,and hemoglobin A1c(HbA1c),the multiple regression analysis showed that FGF23,endocan,TNFR1,and SLPI significantly correlated with eGFR.Conclusions:FGF23,endocan,TNFR1,and SLPI are elevated in advanced DKD compared with early stage,and they may take part in the pathogenesis and progression of DKD.Our study provides useful biomarkers for predicting the appearance of damp-heat syndrome,including FGF23,endocan,TNFR1,and SLPI.
文摘目的:研究清热除湿汤治疗湿热内蕴型面部脂溢性皮炎(SD)患者的疗效及对面部皮肤屏障功能、血清白细胞介素2(IL-2)、白细胞介素6(IL-6)、干扰素-γ(INF-γ)水平的影响。方法:选取2020年5月~2022年5月在本院就诊的82例面部SD(湿热内蕴型)患者,应用简单随机分组法分为试验组和对照组,各41例。对照组进行西药治疗,试验组此基础上采用清热除湿汤治疗,比较两组患者疗效、中医证候积分、皮肤屏障功能、炎性因子等。结果:试验组治疗总有效率高于对照组( P <0.05);治疗后,红色丘疹、油腻性鳞屑或斑片、瘙痒等主次症及总评分均低于对照组( P <0.05);治疗后,试验组角质层含水量(SCH)、表皮脂质含量、皮肤PH高于对照组,经皮肤水分流失量(TEWL)低于对照组( P <0.05);试验组治疗后IL-2、INF-γ水平高于对照组,IL-6水平低于对照组( P <0.05)。结论清热除湿汤治疗湿热内蕴型面部SD患者,能够提升治疗效果,缓解SD相关症状,改善面部皮肤屏障功能指标和血清炎性因子水平。
文摘目的调查混合痔外剥内扎术后患者尿潴留(UR)的发生现状及影响因素。方法选择2019年4月—2022年4月医院收治的行外剥内扎术的混合痔手术患者224例为调查对象,收集患者临床资料,采用一般情况调查表、Zung焦虑自评量表(SAS)和视觉模拟评分量表(VAS)对患者进行问卷调查,根据患者术后有无UR的发生分为病例组和对照组。采用单因素和多因素Logistic回归分析影响混合痔行外剥内扎术患者术后发生UR的因素。结果发放调查问卷224份,回收有效问卷206份,有效回收率为91.96%,其中病例组74例,对照组132例。单因素分析结果表明,患者性别、年龄、合并糖尿病、合并泌尿系统感染、术前焦虑状况、术后发生便秘、麻醉方式、术后2 h VAS评分、麻醉解除时间、输液量、术后首次排尿时间、手术操作、高密度脂蛋白胆固醇(HDL-C)、血糖(Glu)和白细胞(WBC)水平是影响患混合痔行外剥内扎术患者术后UR发生的因素(P<0.05)。多因素Logistic回归分析结果显示,腰硬联合麻醉(OR=2.247,95%CI=1.257~4.015,P=0.006)、高龄(OR=1.539,95%CI=1.364~1.736,P<0.001)、泌尿系统感染(OR=2.709,95%CI=1.258~5.836,P=0.011)、术前焦虑(OR=2.229,95%CI=1.245~3.990,P=0.007)、术后2 h VAS评分(高)(OR=2.833,95%CI=2.080~3.859,P<0.001)、术后发生便秘(OR=5.104,95%CI=2.733~9.534,P<0.001)、手术操作(不合理)(OR=9.333,95%CI=4.818~18.080,P<0.001)、Glu(高)(OR=4.425,95%CI=2.694~7.268,P<0.001)和HDL-C(低)(OR=0.001,95%CI=0.000~0.008,P<0.001)会导致混合痔行外剥内扎术患者发生UR的风险增高。结论护理人员应高度重视混合痔患者在接受外剥内扎术后出现尿潴留的情况,根据患者的高龄、泌尿系统感染、术前焦虑、腰硬联合麻醉、术后疼痛严重、术后发生便秘、血糖高、高密度脂蛋白胆固醇低等影响因素,制订相应的护理干预措施,精心操作手术,以预防混合痔行外剥内扎术后患者出现尿潴留。
