Intervertebral disk degeneration （IDD） is strongly associated with genetic predisposition and environmental susceptibility. Several studies been conducted to investigate the potential asso- ciation between IDD and F...Intervertebral disk degeneration （IDD） is strongly associated with genetic predisposition and environmental susceptibility. Several studies been conducted to investigate the potential asso- ciation between IDD and FokI polymorphism located in the gene encoding the vitamin D receptor （VDR）, and inconsistent conclusions had been reached among different ethnic populations. In order to assess the association between the FokI polymorphism and the risk of IDD, we performed a comprehensive and systematic meta-analysis. Candidate articles were retrieved from PubMed, EMBASE, China National Knowledge Infrastructure （CNKI）, and China Biology Medical （CBM） with strict inclusion criteria in January 2015. Among the 54 articles that were retrieved, only eight studies met the inclusion criteria. The pooled data analysis based on allele contrast, homozy- gore, heterozygote, dominant, and recessive models revealed no significant correlation between the FokI polymorphism and the risk of IDD. However, when stratified by ethnicity, significant associ- ations were detected for Hispanics based on allele contrast （OR = 1.395, 95% CI = 1.059-1.836, P = 0.018）, homozygote （OR = 1.849, 95% CI = 1.001-3.416, P = 0.049）, heterozygote （OR = 1.254, 95% CI = 1.049-1.498, P = 0.013）, and dominant （OR = 1.742, 95% CI = 1.174-2.583, P = 0.006） models, and for Asians using the dominant model （OR = 1.293, 95% CI = 1.025-1.632, P --- 0.030）, whereas there is no significant association detected for Caucasians. In conclusion, FokI polymorpbism is not generally associated with IDD, but there is increased risk for IDD in Hispanics and Asians carrying FokI allele T.展开更多
背景:人类全基因组序列研究发现很多基因突变会导致椎间盘结构改变以及退变风险的提高。目的:使用Meta分析方法评价COL9A2基因rs12077871,rs12722877,rs7533552位点多态性与椎间盘退变的相关性。方法:检索PubMed、Web of Science、中国...背景:人类全基因组序列研究发现很多基因突变会导致椎间盘结构改变以及退变风险的提高。目的:使用Meta分析方法评价COL9A2基因rs12077871,rs12722877,rs7533552位点多态性与椎间盘退变的相关性。方法:检索PubMed、Web of Science、中国知网数据库以及万方数据库,收集COL9A2基因rs12077871,rs12722877,rs7533552位点多态性与椎间盘退变的相关性的研究,检索时间截止至2018年7月。根据STREGA原则评价纳入研究质量,共6篇文献符合既定纳入和排除标准,共计2 874名研究对象纳入研究。主要对椎间盘退变组(病例组)及非椎间盘退变组(对照组)的不同等位基因及基因型的分布特征,使用Stata12.0软件进行Meta分析。结果与结论:(1)分析结果显示:病例组和对照组COL9A2基因rs12077871,rs12722877,rs7533552位点等位基因分布特征、基因型分布特征与椎间盘退变的相关性差异无统计学意义;(2)目前证据显示亚洲人群中COL9A2基因rs12077871,rs12722877,rs7533552位点多态性与椎间盘退变尚无明显相关性,仍需多中心、大样本的高质量病例-对照研究进一步验证Meta分析所得到的结论。展开更多
背景:前期研究发现,腰突颗粒可以通过延缓人髓核细胞的退变,起到保护人髓核细胞的作用。临床发现,其治疗腰椎间盘突出疗效满意。目的:探讨经验方腰突颗粒对兔腰椎间盘退变模型Fas/Fas L基因表达的影响,进一步阐明腰突颗粒防治腰椎间盘...背景:前期研究发现,腰突颗粒可以通过延缓人髓核细胞的退变,起到保护人髓核细胞的作用。临床发现,其治疗腰椎间盘突出疗效满意。目的:探讨经验方腰突颗粒对兔腰椎间盘退变模型Fas/Fas L基因表达的影响,进一步阐明腰突颗粒防治腰椎间盘退变的作用机制。方法:将20只新西兰大白兔采用微创针刺旋切法制备兔腰椎间盘退变模型成功后,随机分为生理盐水组、低剂量组、中剂量组、高剂量组(n=5)。生理盐水组予以10 m L生理盐水灌胃,低剂量组、中剂量组、高剂量组分别予以腰突颗粒水煎剂10,20,40 m L灌胃,灌胃次数为2次/d,持续灌胃21 d。所有新西兰大白兔在相同条件下饲养,灌胃干预完成后,对各组大白兔腰椎髓核中的Fas/Fas L基因表达水平进行检测。结果与结论:(1)新西兰大白兔经微创针刺旋切法造模12周后行MRI检查证实椎间盘信号强度减弱,并见纤维环断裂,椎间盘向后突;(2)Masson染色可见纤维环细胞排列紊乱,甚至断裂,番红O染色见髓核细胞明显减少;(3)与生理盐水组相比,低剂量组、中剂量组、高剂量组椎间盘组织的Fas m RNA、Fasl m RNA表达均降低,且随着灌服腰突颗粒剂量的增加,Fas m RNA、Fasl m RNA相对表达量逐渐降低(P<0.05);(4)结果说明,腰突颗粒可降低兔腰椎兔腰椎间盘退变模型中髓核Fas/Fas L基因表达,进而延缓兔腰椎间盘退变。展开更多
基金supported by the Science & Technology Commission of Shanghai Municipality, China (Grant No. 11DZ191109)the National Natural Science Foundation of China (Grant No. 30872611)
文摘Intervertebral disk degeneration （IDD） is strongly associated with genetic predisposition and environmental susceptibility. Several studies been conducted to investigate the potential asso- ciation between IDD and FokI polymorphism located in the gene encoding the vitamin D receptor （VDR）, and inconsistent conclusions had been reached among different ethnic populations. In order to assess the association between the FokI polymorphism and the risk of IDD, we performed a comprehensive and systematic meta-analysis. Candidate articles were retrieved from PubMed, EMBASE, China National Knowledge Infrastructure （CNKI）, and China Biology Medical （CBM） with strict inclusion criteria in January 2015. Among the 54 articles that were retrieved, only eight studies met the inclusion criteria. The pooled data analysis based on allele contrast, homozy- gore, heterozygote, dominant, and recessive models revealed no significant correlation between the FokI polymorphism and the risk of IDD. However, when stratified by ethnicity, significant associ- ations were detected for Hispanics based on allele contrast （OR = 1.395, 95% CI = 1.059-1.836, P = 0.018）, homozygote （OR = 1.849, 95% CI = 1.001-3.416, P = 0.049）, heterozygote （OR = 1.254, 95% CI = 1.049-1.498, P = 0.013）, and dominant （OR = 1.742, 95% CI = 1.174-2.583, P = 0.006） models, and for Asians using the dominant model （OR = 1.293, 95% CI = 1.025-1.632, P --- 0.030）, whereas there is no significant association detected for Caucasians. In conclusion, FokI polymorpbism is not generally associated with IDD, but there is increased risk for IDD in Hispanics and Asians carrying FokI allele T.
文摘背景:人类全基因组序列研究发现很多基因突变会导致椎间盘结构改变以及退变风险的提高。目的:使用Meta分析方法评价COL9A2基因rs12077871,rs12722877,rs7533552位点多态性与椎间盘退变的相关性。方法:检索PubMed、Web of Science、中国知网数据库以及万方数据库,收集COL9A2基因rs12077871,rs12722877,rs7533552位点多态性与椎间盘退变的相关性的研究,检索时间截止至2018年7月。根据STREGA原则评价纳入研究质量,共6篇文献符合既定纳入和排除标准,共计2 874名研究对象纳入研究。主要对椎间盘退变组(病例组)及非椎间盘退变组(对照组)的不同等位基因及基因型的分布特征,使用Stata12.0软件进行Meta分析。结果与结论:(1)分析结果显示:病例组和对照组COL9A2基因rs12077871,rs12722877,rs7533552位点等位基因分布特征、基因型分布特征与椎间盘退变的相关性差异无统计学意义;(2)目前证据显示亚洲人群中COL9A2基因rs12077871,rs12722877,rs7533552位点多态性与椎间盘退变尚无明显相关性,仍需多中心、大样本的高质量病例-对照研究进一步验证Meta分析所得到的结论。
文摘背景:前期研究发现,腰突颗粒可以通过延缓人髓核细胞的退变,起到保护人髓核细胞的作用。临床发现,其治疗腰椎间盘突出疗效满意。目的:探讨经验方腰突颗粒对兔腰椎间盘退变模型Fas/Fas L基因表达的影响,进一步阐明腰突颗粒防治腰椎间盘退变的作用机制。方法:将20只新西兰大白兔采用微创针刺旋切法制备兔腰椎间盘退变模型成功后,随机分为生理盐水组、低剂量组、中剂量组、高剂量组(n=5)。生理盐水组予以10 m L生理盐水灌胃,低剂量组、中剂量组、高剂量组分别予以腰突颗粒水煎剂10,20,40 m L灌胃,灌胃次数为2次/d,持续灌胃21 d。所有新西兰大白兔在相同条件下饲养,灌胃干预完成后,对各组大白兔腰椎髓核中的Fas/Fas L基因表达水平进行检测。结果与结论:(1)新西兰大白兔经微创针刺旋切法造模12周后行MRI检查证实椎间盘信号强度减弱,并见纤维环断裂,椎间盘向后突;(2)Masson染色可见纤维环细胞排列紊乱,甚至断裂,番红O染色见髓核细胞明显减少;(3)与生理盐水组相比,低剂量组、中剂量组、高剂量组椎间盘组织的Fas m RNA、Fasl m RNA表达均降低,且随着灌服腰突颗粒剂量的增加,Fas m RNA、Fasl m RNA相对表达量逐渐降低(P<0.05);(4)结果说明,腰突颗粒可降低兔腰椎兔腰椎间盘退变模型中髓核Fas/Fas L基因表达,进而延缓兔腰椎间盘退变。
