With a view to finding out precisely how small peptides recognize a particular binding site of DNA, we have accomplished DNA binding studies of two peptides, H-Tyr-Arg-OH (YR) and H-Gly-Gly-His-OH (GGH) by using measu...With a view to finding out precisely how small peptides recognize a particular binding site of DNA, we have accomplished DNA binding studies of two peptides, H-Tyr-Arg-OH (YR) and H-Gly-Gly-His-OH (GGH) by using measurements in comparison with the binding between DNA and Hoechst 33258. The inhibition mode by YR and GGH to DNA binding of Hoechst 33258 was analyzed by Lineweaver-Burk plot which shows the plot of typical competitive inhibition at concentration of Hoechst 33258 from 3.66 ( 10-9 mol / L to 1.09 ( 10-8 mol / L. And it is concluded that YR binds to DNA in its minor groove (AT rich regions) with a binding constant K = 1.02 ( 108 (mol / L)-1. The GGH(s specificity is reduced at high concentration because it can also bind GC base pair.展开更多
The competitiveness of navel orange characteristic industrial cluster lies in three aspects. First is the competitiveness of specific resources. The second is the competitiveness of market. The third is the competitiv...The competitiveness of navel orange characteristic industrial cluster lies in three aspects. First is the competitiveness of specific resources. The second is the competitiveness of market. The third is the competitiveness of manpower, organization and system background .An evaluation model on competitiveness of navel orange characteristic industry cluster is established with AHP in this article. The conclusion is that the competitive essence of navel orange characteristic industry cluster refers to the quality of the soil, the climate, the geographical position and the transportation condition which the cluster has. Compared with the navel orange characteristic industry clusters in western developed countries, especially in USA, the disparity of competitiveness of Gannan navel orange characteristic industrial cluster （GNOCIC for short） is obvious from a global perspective. Compared with the navel orange characteristic industry competitive advantage of GNOCIC is extremely obvious. clusters in Three Gorges, and Sichuan Province, the competitive advantage of GNOCIC is extremely obvious.展开更多
文摘With a view to finding out precisely how small peptides recognize a particular binding site of DNA, we have accomplished DNA binding studies of two peptides, H-Tyr-Arg-OH (YR) and H-Gly-Gly-His-OH (GGH) by using measurements in comparison with the binding between DNA and Hoechst 33258. The inhibition mode by YR and GGH to DNA binding of Hoechst 33258 was analyzed by Lineweaver-Burk plot which shows the plot of typical competitive inhibition at concentration of Hoechst 33258 from 3.66 ( 10-9 mol / L to 1.09 ( 10-8 mol / L. And it is concluded that YR binds to DNA in its minor groove (AT rich regions) with a binding constant K = 1.02 ( 108 (mol / L)-1. The GGH(s specificity is reduced at high concentration because it can also bind GC base pair.
文摘The competitiveness of navel orange characteristic industrial cluster lies in three aspects. First is the competitiveness of specific resources. The second is the competitiveness of market. The third is the competitiveness of manpower, organization and system background .An evaluation model on competitiveness of navel orange characteristic industry cluster is established with AHP in this article. The conclusion is that the competitive essence of navel orange characteristic industry cluster refers to the quality of the soil, the climate, the geographical position and the transportation condition which the cluster has. Compared with the navel orange characteristic industry clusters in western developed countries, especially in USA, the disparity of competitiveness of Gannan navel orange characteristic industrial cluster （GNOCIC for short） is obvious from a global perspective. Compared with the navel orange characteristic industry competitive advantage of GNOCIC is extremely obvious. clusters in Three Gorges, and Sichuan Province, the competitive advantage of GNOCIC is extremely obvious.
