This study explores the efficacy of advanced antibiotic compounds against P. aeruginosa, focusing on Antibiotic B, an enhanced derivative of Ceftriaxone. The study measured the intracellular uptake of Antibiotic B and...This study explores the efficacy of advanced antibiotic compounds against P. aeruginosa, focusing on Antibiotic B, an enhanced derivative of Ceftriaxone. The study measured the intracellular uptake of Antibiotic B and introduced a novel adjuvant, Influximax, which augmented its antibacterial activity. Results showed a diminished potential for resistance emergence with Antibiotic B, particularly when used in combination with Influximax. The study suggests that optimizing antibiotic delivery into bacterial cells and leveraging syner-gistic adjuvant combinations can enhance drug resistance combat. .展开更多
The VirE2-interaction protein 1(VIP1)serves as a regulator of mitogen-activated protein kinase 3(MPK3)-mediated stress gene modulation under biotic stress,which in turn activates the MPK3 pathway in Arabidopsis.The mo...The VirE2-interaction protein 1(VIP1)serves as a regulator of mitogen-activated protein kinase 3(MPK3)-mediated stress gene modulation under biotic stress,which in turn activates the MPK3 pathway in Arabidopsis.The mode of action of the VIP1 protein in Populus in response to biotic stress remains unknown.In this study,we cloned the full-length cDNA of the PtVIP1 gene from Populus trichocarpa(accession number of GenBank:KY793105).The VIP1 protein harboured a conserved bZIP(basic leucine zipper)domain located in the C-terminus.The VIP1 subcellular localization assay indicated that the VIP1 protein was present in the cytoplasm and nucleus under normal conditions,and that an increase in the amount of the protein in the nucleus occurred after treatment with flg22,the elicitor-active epitope of flagellin which triggers the innate immune response in plants.Transgenic Populus plants overexpressing VIP1 genes(PtVIP1 of Populus;or AtVIP1 of Arabidopsis,as positive control)were generated to investigate the role of VIP1 in vivo.The expression of poplar pathogenesis-related protein 1(PR1)genes was upregulated in transgenic-PtVIP1 or AtVIP1 poplar plants.The transgenic poplar plants overexpressing PtVIP1 or AtVIP1 also showed enhanced resistance to Brenneria salicis infection.These results suggest that the VIP1 protein accumulates in the nucleus in response to biotic stress,and that the pathogen resistance of transgenic VIP1 poplar may be associated with the induced expression of PR1 genes in response to pathogen challenge.展开更多
多药耐药相关蛋白1(multidrug resistance-associated protein 1,MRP1)是ATP结合盒转运蛋白超家族的ABCC亚家族成员,在人体内分布广泛,转运底物众多,具有重要的生理学、病理学和药理学功能。除与肿瘤及抗肿瘤药物耐药、炎症性和免疫性...多药耐药相关蛋白1(multidrug resistance-associated protein 1,MRP1)是ATP结合盒转运蛋白超家族的ABCC亚家族成员,在人体内分布广泛,转运底物众多,具有重要的生理学、病理学和药理学功能。除与肿瘤及抗肿瘤药物耐药、炎症性和免疫性疾病、心血管疾病、神经系统疾病等密切相关外,近年来MRP1在感染性疾病中的作用也日益受到关注。该文就MRP1的结构、功能和调控及其与病原体感染和耐药相关性的研究进展作一综述。展开更多
文摘This study explores the efficacy of advanced antibiotic compounds against P. aeruginosa, focusing on Antibiotic B, an enhanced derivative of Ceftriaxone. The study measured the intracellular uptake of Antibiotic B and introduced a novel adjuvant, Influximax, which augmented its antibacterial activity. Results showed a diminished potential for resistance emergence with Antibiotic B, particularly when used in combination with Influximax. The study suggests that optimizing antibiotic delivery into bacterial cells and leveraging syner-gistic adjuvant combinations can enhance drug resistance combat. .
基金supported by the National Natural Science Foundation of China(Grant No.31570639)the Distinguished Young Scholars Fund of Nanjing Forestry University,the Priority Academic Program Development of Nanjing Forestry University the Poplar Germplasm Nursery Project(Jiangsu Provincial Platform for Conservation and Utilizations for Agricultural Germplasm)
文摘The VirE2-interaction protein 1(VIP1)serves as a regulator of mitogen-activated protein kinase 3(MPK3)-mediated stress gene modulation under biotic stress,which in turn activates the MPK3 pathway in Arabidopsis.The mode of action of the VIP1 protein in Populus in response to biotic stress remains unknown.In this study,we cloned the full-length cDNA of the PtVIP1 gene from Populus trichocarpa(accession number of GenBank:KY793105).The VIP1 protein harboured a conserved bZIP(basic leucine zipper)domain located in the C-terminus.The VIP1 subcellular localization assay indicated that the VIP1 protein was present in the cytoplasm and nucleus under normal conditions,and that an increase in the amount of the protein in the nucleus occurred after treatment with flg22,the elicitor-active epitope of flagellin which triggers the innate immune response in plants.Transgenic Populus plants overexpressing VIP1 genes(PtVIP1 of Populus;or AtVIP1 of Arabidopsis,as positive control)were generated to investigate the role of VIP1 in vivo.The expression of poplar pathogenesis-related protein 1(PR1)genes was upregulated in transgenic-PtVIP1 or AtVIP1 poplar plants.The transgenic poplar plants overexpressing PtVIP1 or AtVIP1 also showed enhanced resistance to Brenneria salicis infection.These results suggest that the VIP1 protein accumulates in the nucleus in response to biotic stress,and that the pathogen resistance of transgenic VIP1 poplar may be associated with the induced expression of PR1 genes in response to pathogen challenge.
文摘多药耐药相关蛋白1(multidrug resistance-associated protein 1,MRP1)是ATP结合盒转运蛋白超家族的ABCC亚家族成员,在人体内分布广泛,转运底物众多,具有重要的生理学、病理学和药理学功能。除与肿瘤及抗肿瘤药物耐药、炎症性和免疫性疾病、心血管疾病、神经系统疾病等密切相关外,近年来MRP1在感染性疾病中的作用也日益受到关注。该文就MRP1的结构、功能和调控及其与病原体感染和耐药相关性的研究进展作一综述。
