Objective Thyroid hormones(THs)regulate multiple physiological activities in the liver,including cellular metabolism,differentiation,and cell growth,and play important roles in the pathogenesis of hepatocellular carci...Objective Thyroid hormones(THs)regulate multiple physiological activities in the liver,including cellular metabolism,differentiation,and cell growth,and play important roles in the pathogenesis of hepatocellular carcinoma(HCC).Thyroid peroxidase(TPO)is a key molecule involved in the THs synthesis and signaling pathway.As an epigenetic modification,DNA methylation has a critical role in tumorigenesis with diagnostic potential.However,the connection between THs and DNA methylation has been rarely investigated.Methods The methylation of key TH-related genes was analyzed by in-house epigenome-wide scanning,and we further analyzed the methylation levels of the TPO promotor in 164 sample pairs of HCC and adjacent non-cancerous tissues by Sequenom EpiTYPER assays,and evaluated their clinical implications.Results We identified that the methylation of the TPO promoter was downregulated in the HCC tissues(P<0.0001)with a mean difference ranging from 18.5%to 22.3%.This methylation pattern correlated with several clinical factors,including a multi-satellite tumor,fibrous capsule,and the presence of tumor thrombus.The receiver operator characteristic(ROC)curve analysis further confirmed that the percent methylated reference(PMR)values for TPO were predictive of the tumor[the area under the curve(AUC)ranged from 0.755 to 0.818]and the thrombosis in the HCC patients(the AUC ranged from 0.706 to 0.777).Conclusion These findings demonstrated that epigenetic alterations of TPO,as indicated by the PMR values,were a potential biomarker for HCC patients with tumor thrombosis.展开更多
Horseradish peroxidase (HRP) is generally used as a label enzyme in enzyme immunoassay (EIA).The procedure used for HRP detection in EIA is critical for sensitivity and precision.This paper describes a novel fluorimet...Horseradish peroxidase (HRP) is generally used as a label enzyme in enzyme immunoassay (EIA).The procedure used for HRP detection in EIA is critical for sensitivity and precision.This paper describes a novel fluorimetric assay for horseradish peroxidase (HRP) using sesamol as substrate.The principle of the assay is as follow:sesamol (3,4-methylenedioxy phenol) is reacted enzymatically in the presence of hydrogen peroxide to produce dimeric sesamol.The dimer is fluorescent and can be detected sensitively at ex.347 nm,em.427 nm.The measurable range of HRP was 1.0×10-18 to 1.0×10-15 mol/assay,with a detection limit of 1.0×10-18 mol/assay.The coefficient of variation (CV,n=8) was examined at each point on the standard curve,with a mean CV percentage of 3.8%.This assay system was applied to thyroid stimulating hormone (TSH) EIA using HRP as the label enzyme.展开更多
目的探讨左甲状腺素钠片替代疗法对妊娠期亚临床甲状腺减退(SCH)孕妇甲状腺功能的影响。方法选取2019年6月至2021年5月瑞金市妇幼保健院收治的80例妊娠期SCH孕妇作为研究对象,按照随机信封法分为对照组与观察组,每组40例。对照组给予常...目的探讨左甲状腺素钠片替代疗法对妊娠期亚临床甲状腺减退(SCH)孕妇甲状腺功能的影响。方法选取2019年6月至2021年5月瑞金市妇幼保健院收治的80例妊娠期SCH孕妇作为研究对象,按照随机信封法分为对照组与观察组,每组40例。对照组给予常规治疗,观察组给予左甲状腺素钠片替代疗法。比较两组甲状腺功能[促甲状腺激素(TSH)、抗甲状腺过氧化物酶自身抗体(TPO-Ab)、游离甲状腺素(FT4)]、血糖[空腹血糖(FBG)、餐后2 h血糖(2 h PG)]与维生素D水平、肝功能[总胆红素(TBIL)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)]及胎儿不良结局发生情况。结果治疗后1个月,观察组TSH、TPO-Ab水平均低于对照组,FT4水平高于对照组,差异有统计学意义(P<0.05)。治疗后1个月,观察组FBG、2 h PG水平均低于对照组,维生素D水平高于对照组,差异有统计学意义(P<0.05)。治疗后1个月,观察组TBIL、AST、ALT水平均低于对照组,差异有统计学意义(P<0.05)。治疗后,两组胎儿不良结局发生率比较差异无统计学意义。结论左甲状腺素钠片替代疗法可显著改善妊娠期SCH孕妇的甲状腺功能,避免因甲状腺代谢异常造成的高血糖与维生素D营养异常状态,对肝功能损伤较小,临床使用较为安全。展开更多
文摘Objective Thyroid hormones(THs)regulate multiple physiological activities in the liver,including cellular metabolism,differentiation,and cell growth,and play important roles in the pathogenesis of hepatocellular carcinoma(HCC).Thyroid peroxidase(TPO)is a key molecule involved in the THs synthesis and signaling pathway.As an epigenetic modification,DNA methylation has a critical role in tumorigenesis with diagnostic potential.However,the connection between THs and DNA methylation has been rarely investigated.Methods The methylation of key TH-related genes was analyzed by in-house epigenome-wide scanning,and we further analyzed the methylation levels of the TPO promotor in 164 sample pairs of HCC and adjacent non-cancerous tissues by Sequenom EpiTYPER assays,and evaluated their clinical implications.Results We identified that the methylation of the TPO promoter was downregulated in the HCC tissues(P<0.0001)with a mean difference ranging from 18.5%to 22.3%.This methylation pattern correlated with several clinical factors,including a multi-satellite tumor,fibrous capsule,and the presence of tumor thrombus.The receiver operator characteristic(ROC)curve analysis further confirmed that the percent methylated reference(PMR)values for TPO were predictive of the tumor[the area under the curve(AUC)ranged from 0.755 to 0.818]and the thrombosis in the HCC patients(the AUC ranged from 0.706 to 0.777).Conclusion These findings demonstrated that epigenetic alterations of TPO,as indicated by the PMR values,were a potential biomarker for HCC patients with tumor thrombosis.
文摘Horseradish peroxidase (HRP) is generally used as a label enzyme in enzyme immunoassay (EIA).The procedure used for HRP detection in EIA is critical for sensitivity and precision.This paper describes a novel fluorimetric assay for horseradish peroxidase (HRP) using sesamol as substrate.The principle of the assay is as follow:sesamol (3,4-methylenedioxy phenol) is reacted enzymatically in the presence of hydrogen peroxide to produce dimeric sesamol.The dimer is fluorescent and can be detected sensitively at ex.347 nm,em.427 nm.The measurable range of HRP was 1.0×10-18 to 1.0×10-15 mol/assay,with a detection limit of 1.0×10-18 mol/assay.The coefficient of variation (CV,n=8) was examined at each point on the standard curve,with a mean CV percentage of 3.8%.This assay system was applied to thyroid stimulating hormone (TSH) EIA using HRP as the label enzyme.
文摘目的探讨左甲状腺素钠片替代疗法对妊娠期亚临床甲状腺减退(SCH)孕妇甲状腺功能的影响。方法选取2019年6月至2021年5月瑞金市妇幼保健院收治的80例妊娠期SCH孕妇作为研究对象,按照随机信封法分为对照组与观察组,每组40例。对照组给予常规治疗,观察组给予左甲状腺素钠片替代疗法。比较两组甲状腺功能[促甲状腺激素(TSH)、抗甲状腺过氧化物酶自身抗体(TPO-Ab)、游离甲状腺素(FT4)]、血糖[空腹血糖(FBG)、餐后2 h血糖(2 h PG)]与维生素D水平、肝功能[总胆红素(TBIL)、天冬氨酸转氨酶(AST)、丙氨酸转氨酶(ALT)]及胎儿不良结局发生情况。结果治疗后1个月,观察组TSH、TPO-Ab水平均低于对照组,FT4水平高于对照组,差异有统计学意义(P<0.05)。治疗后1个月,观察组FBG、2 h PG水平均低于对照组,维生素D水平高于对照组,差异有统计学意义(P<0.05)。治疗后1个月,观察组TBIL、AST、ALT水平均低于对照组,差异有统计学意义(P<0.05)。治疗后,两组胎儿不良结局发生率比较差异无统计学意义。结论左甲状腺素钠片替代疗法可显著改善妊娠期SCH孕妇的甲状腺功能,避免因甲状腺代谢异常造成的高血糖与维生素D营养异常状态,对肝功能损伤较小,临床使用较为安全。
