Four 20-membered N_2S_4-monoazathiacrown ethers have been synthesized and explored as neutral ionophores for Ag^+-selective electrodes.Potentiometric responses reveal that the flexibility of the ligands has great effe...Four 20-membered N_2S_4-monoazathiacrown ethers have been synthesized and explored as neutral ionophores for Ag^+-selective electrodes.Potentiometric responses reveal that the flexibility of the ligands has great effect on the selectivity and sensitivity to Ag^+ ions.The electrode based on ionophore 9,10,20,25-tetrahydro-5H,12H-tribenzo[b,n,r][1,7,10,16,4,13]tetrathiadiaza cycloicosine 6,13-(7H,14H)-dione(C) with 2-nitrophenyl octyl ether(o-NPOE) as solvent in a poly(vinyl chloride)(PVC) membrane matrix sho...展开更多
Timing of vegetal-endodermal cell determination in amphioxus embryos remains uncertain. We tentatively testal effects of A23187, the calcium ionophore, on the deveopment of vegetal blastomeres isolated at the 16-cell ...Timing of vegetal-endodermal cell determination in amphioxus embryos remains uncertain. We tentatively testal effects of A23187, the calcium ionophore, on the deveopment of vegetal blastomeres isolated at the 16-cell stage. It was found that when vegetal blastomres committed to endodermwere treated with A23187 prior to gastrulation, they were transformed into ectodermal cells as evidenced by the cell morphology and function characteristic of epidermis. Howver, the developmental fate of the sam blastomeres untreated or treated with DMSO at the same stage or of those treated with A23187 after gastrulation remained unchanged. Thus, vegetal-endodermal cells in amphioxus embryos are not irreversibly deermined before the gastrula stage, and artificial incarease in intracelluar Ca2+ concentration can induce transdetermination of the predetermined endodermal cells into ectodermal cells.展开更多
A new type of di benzoyl tartaric acid selective electr ode has been developed. Three double\| arm calixarene derivatives were emp loyed as the neutral ionophores. The poly(vinyl chloride) me...A new type of di benzoyl tartaric acid selective electr ode has been developed. Three double\| arm calixarene derivatives were emp loyed as the neutral ionophores. The poly(vinyl chloride) membrane electrode containing an amide derivative of ca lixarene as the neutral carrier an d a dibutyl phthalate as the plastici zer exhibited the highest sensitivity for dibenzoyl tartaric acid. The slop e of linear portion was 27.8 mV per c oncertration decade. The electrode has a fast response and a long lifetime .展开更多
A thiocyanate ion selective poly(aniline) solid contact electrode based on manganese complex of N,N’-bis-(4-phenylazosalicylidene)-o-phenylene diamine ionophore was successfully developed. The electrode exhibits a go...A thiocyanate ion selective poly(aniline) solid contact electrode based on manganese complex of N,N’-bis-(4-phenylazosalicylidene)-o-phenylene diamine ionophore was successfully developed. The electrode exhibits a good linear response of 58.1 mV/decade (at 20?C ± 0.2?C, r2 = 0.998) with in the concentration range of 1 × 10–1.0 ~ 1 × 10–5.8 M thiocyanate solution. The composition of this electrode was: ionophore 0.040, polyvinylchloride 0.300, dibutylphthalate 0.660 (mass). This dibutylphthalate plasticizer provides the best response characteristics. The electrode shows good selectivity for thiocyanate ion in comparison with any other anions and is suitable for use with aqueous solutions of pH 4.0 ~ 6.0. The standard deviations of the measured emf difference were ±1.70 and ±2.01 mV for thiocyanate sample solutions of 1.0 × 10–2 M and 1.0 × 10–3 M, respectively. The stabilization time was less than 170 sec. and response time was less than 17 sec.展开更多
The release of mediators from mast cells is a model for cell secretion and is an in-vitro index for immediate hypersensitivity reactions. Calcium influx is generally accepted to be the primary biochemicalevent in mast...The release of mediators from mast cells is a model for cell secretion and is an in-vitro index for immediate hypersensitivity reactions. Calcium influx is generally accepted to be the primary biochemicalevent in mast cell activation.We studied the effect of the calcium ionophore A 23187 and calcium channelblockers,nifedipine and verapamil, in triggering the activation of rat peritoneal mast cells.At suitableconcentration nifedipine and verapamil have had the inhibition effect in the IgE-dependent roaction.展开更多
Novel solid-contact perchlorate sensors based on cobalt phthalocyanine-C-monocarboxylic acid (I), and cobalt phthalocyanine-C,C,C,C-tetracarboxylic acid (II) as free ionophores and covalently attached to polyacryla- m...Novel solid-contact perchlorate sensors based on cobalt phthalocyanine-C-monocarboxylic acid (I), and cobalt phthalocyanine-C,C,C,C-tetracarboxylic acid (II) as free ionophores and covalently attached to polyacryla- mide (PAA)—ionophores III and IV, respectively were prepared. The all solid-state sensors were constructed by the application of a thin film of a polymer cocktail containing a phthalocyanine ionophore and cetyltrimethylammonium bromide (CTMAB) as a lipophilic cationic additive onto a gold electrode precoated with the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) as an ion and electron transducer. The sensor with 10.3% of ionophore (III) covalently attached to plasticizer-free poly (butyl methacrylate-co-do- decyl methacrylate) (PBDA) exhibited a good selectivity for perchlorate and discriminated many ions, in- cluding F–, Cl–, Br–, I–, SCN–, , S2– and . The covalent attachment of the ionophore to the polymer resulted in a near-Nernstian anionic slope of –62.3 mV/decade whereas a super-Nernstian slope of –79.9 mV/ decade was obtained for the free ionophore. The sensor covered a linear concentration range of 5 × 10–9 - 1 × 10–2 mol?L–1 with a lower detection limit (LDL) of 1 × 10–9 mol?L–1 and gave a stable response over a pH range of 4 - 10.5. The all-solid state sensors were utilized for the selective flow injection potentiometric determination of perchlorate in natural water and human urine samples in the nanomolar concentration range.展开更多
<strong>Objectives:</strong><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> Evaluate the effect of ...<strong>Objectives:</strong><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> Evaluate the effect of artificial oocyte activation (AOA) using calcium ionophore (A23187) on the rate of fertilization and cleavage of embryos in surgically retrieved sperm of patients with non-obstructive azoospermia undergoing intracytoplasmic sperm injection (ICSI).</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">Study design: </span></b></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">This study was conducted on 60 infertile couples undergoing ICSI cycles as a randomized controlled parallel group’s experimental study in a private IVF center in Egypt from January 2018 to July 2019. ICSI cycles were divided into two g</span><span style="font-family:Verdana;">roups: Group A: includes 30 ICSI patients with surgically retrieved sperms of non-obstructive azoospermia treated with calcium ionophore (A23187). Group C/Control: includes 30 ICSI patients with surgically retrieved sperms of non-obstructive azoospermia non-treated with calcium ionophore (A23187)</span><span style="font-family:Verdana;">. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">There was no statistical difference between both groups regarding the fertilization rate (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> = 0.853). There was no statistical difference between them regarding implantation rate (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> = 0.237). The percentage of Class A embryos in the ca ionophore group was 81.7%, while it was 82.8% in the control group. There was insignificant difference between them (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> = 0.782). There was no statistical significant difference between the two groups regarding the clinical pregnancy rate</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">,</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> it was (56.7%) in the ca ionophore group while it was (53.3%) in the control group.</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">Conclusion: </span></b></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">AOA by Ca</span><sup><span style="font-family:Verdana;">2++</span></sup></span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">ionophore didn’t improve the outcome of ICSI cycle in cases of non obstructive azoospermia in terms of fertilization, implantation and pregnancy rate.</span></span></span>展开更多
Ionophore can prominently improve the ion permeability of cell membrane and disrupt cellular ion homeostasis.Most studies regarding ionophore facilitating ion transmembrane transport focus on artificial liquid-liquid ...Ionophore can prominently improve the ion permeability of cell membrane and disrupt cellular ion homeostasis.Most studies regarding ionophore facilitating ion transmembrane transport focus on artificial liquid-liquid interfaces,which have large difference from the actual environment of cell membrane.Here,we construct a supported lipid bilayeron a gold nanoparticles film modified ZnSe prism as an appropriate model of cell membrane to investigate the dynamic of the ion transport facilitated by ionophore using surface enhanced infrared absorption spectroscopy(SEIRAS).We find that the ion transmembrane transport consists of two steps:The ion transmembrane transport starts with the association/disassociation between ion and ionophore at the edge of lipid bilayer;The second step is the transfer of ion-ionophore complex across lipid bilayer.Our results show that the complex transfer across the lipid bilayer is the rate determining step.展开更多
We use the extended gate field effect transistor (EGFET)as the structure of the chlorine ion sensor,and the chlorine ion ionophores (ETH9033 and TDDMAC1)are incorporated into solvent polymeric membrane (PVC/DOS),then ...We use the extended gate field effect transistor (EGFET)as the structure of the chlorine ion sensor,and the chlorine ion ionophores (ETH9033 and TDDMAC1)are incorporated into solvent polymeric membrane (PVC/DOS),then the chlorine ion selective membrane is formed on the sensing window,and the fabrication of the EGFET chlorine ion sensing device is completed.The surface potential on the sensing membrane of the EGFET chlorine ion sensing device will be changed in the different chlorine ion concentration solutions,then changes further gate voltage and drain current to detect chlorine ion concentration.We will study non-ideal effects such as temperature,hysteresis and drift effects for the EGFET chlorine ion sensing device in this paper,these researches will help us to improve the sensing characteristics of the EGFET chlorine ion sensing device.展开更多
A new chemically modified carbon paste electrode for cesium(I) ion determination based on potassium zinc hexacyan-oferrate (PZHCF) as an ionophore was prepared. The electrode exhibits a Nernstian response for Cs(I) io...A new chemically modified carbon paste electrode for cesium(I) ion determination based on potassium zinc hexacyan-oferrate (PZHCF) as an ionophore was prepared. The electrode exhibits a Nernstian response for Cs(I) ions over a wide concentration range from 1 × 10-6 to 1 × 10-1 mol·L-1 with a slope of 58 ± 0.5 mV·decade-1. It has a response time of about 35 s and can be used for a period of 3 months with good reproducibility. Detection limit obtained in the optimal conditions was 3 × 10-7 mol·L-1. The potentiometric response is independent of the pH of the solution in the pH range 4.0 - 8.0. The electrode possesses the advantages of low resistance, fast response over a variety of other cations. The proposed electrode is applied as a sensor for the determination of Cs(I) ion concentration in different samples solutions. The results showed a good correlation with the data obtained by atomic absorption spectrometric method.展开更多
A modified method of preparing crown functionalized linear polysiloxane has been described. 4'-Allyldibenzo-18-crown-6 was subjected to hydrosilylation with methyldichlorosilane, followed by polycondensation with ...A modified method of preparing crown functionalized linear polysiloxane has been described. 4'-Allyldibenzo-18-crown-6 was subjected to hydrosilylation with methyldichlorosilane, followed by polycondensation with silanol-terminated polydimethylsiloxane to give the title crown functionalized linear polysiloxane. The transport properties of sodium, potassium, and ammonium salt through a bulk liquid membrane system using the new type of crown functionalized linear polysiloxane as a carrier were investigated. It is worthy to point out that the carrier can be used repeatedly at least six runs with no apparent change in the transport rate of potassium ion.展开更多
Objective: To study the role of apoptosis in tumor cell of malignant glioma death following treatment with hyperthermia and calcium ionophore. Methods: The apoptosis induced by hyperthermia and calcium ionophore, A231...Objective: To study the role of apoptosis in tumor cell of malignant glioma death following treatment with hyperthermia and calcium ionophore. Methods: The apoptosis induced by hyperthermia and calcium ionophore, A23187, in human glioblastoma cell line TJ905 and murine glioblastoma G422 was evaluated by characteristic findings in DNA agarose gel electrophresis, ultrastructural examination and flow cytometric analysis. Results: Apoptosis could be induced by moderate hyperthermia, but not by mild hyperthermia, calcium ionophore enhanced significantly the effect of mild hyperthermia on the induction of apoptosis. Conclusion: This result indicates that apoptotic cell death is one of the mechanisms of hyperthermic therapy for malignant glioma and taking measures to increase the cytolic calcium may enhance the effect of hyperthermia.展开更多
Ionophores consist of molecules which surround and carry positive metal ions and other ions through biological membranes. One classe of ionophores which we have been developing contains dipeptides which are encouraged...Ionophores consist of molecules which surround and carry positive metal ions and other ions through biological membranes. One classe of ionophores which we have been developing contains dipeptides which are encouraged to become part of a ring because of possible hydrogen bond formation between the 2-hydroxy on the phenyl group and carboxyl group (COOH) of the final amide proline. Formation of a ring should increase the complexation ability of the ionophore. We report that the synthesis of N-(2-hydroxyl-1-phenoxyacetyl) prolyproline(1), a novel ionophore is prepared from activated 2-acetoxy phenoxyacetic acid (3a) and the appropriate dipeptide ester using coupling methods such as dicyclohexyl carbodiimide with hydroxyben-ztriazole or carbonyl diimidazole.展开更多
The release of mediators from mast cells is a model for cell secretion and is an in-vitro index for immediate hypersensitivity reactions. Calcium influx is generally accepted to be the primary biochemicalevent in mast...The release of mediators from mast cells is a model for cell secretion and is an in-vitro index for immediate hypersensitivity reactions. Calcium influx is generally accepted to be the primary biochemicalevent in mast cell activation.We studied the effect of the calcium ionophore A 23187 and calcium channelblockers,nifedipine and verapamil, in triggering the activation of rat peritoneal mast cells.At suitableconcentration nifedipine and verapamil have had the inhibition effect in the IgE-dependent roaction.展开更多
Beef cattle producers in the North America have a variety of production and marketing options and must choose the best production system for their situation. This review describes considerations involved in choosing b...Beef cattle producers in the North America have a variety of production and marketing options and must choose the best production system for their situation. This review describes considerations involved in choosing between feeding cattle conventionally versus feeding them in programs that prohibit the use of certain technologies. Data from peer-reviewed journals, extension publications, nutritional consultants, governmental organizations, and feed companies were used to construct this review. Most cattle in North America are fed in conventional production systems. Conventional beef production systems typically use steroidal implants, ionophores, and beta-adrenergic agonists to improve animal productivity;as well as feed grade and injectable antimicrobials to control, treat or prevent disease and improve animal health. These technologies have been shown to lower the cost of production, allowing for beef to be competitive in the global protein market. Some consumers have expressed a preference for beef produced without these technologies. These “All-natural” (AN) cattle may bring a premium price in the market. The economic impact of differing productions systems can be described in relation to 1) cost of production, 2) operating costs of the feedlot, 3) price paid for feeder calves, and 4) price received for fed cattle. Conventional production provides the most favorable outcome for factors 1, 2, and 3, while AN production provides the most favorable outcome for item 4. There are also industry wide and societal aspects related to differing beef production systems related to health and safety of beef, land use, and cost of production allowing for a greater share of the global protein market. Technologies used in conventional production are critical tools to North American beef production. Differences in efficiencies between each type of non-conventional production systems must be re-captured in added premiums when cattle are marketed and sold. Premiums for AN cattle are enticing, but the true differences in the cost of production between the AN and conventional cattle must be evaluated in order for a producer to make the correct decision for their operation.展开更多
The new ferrous-selective modified carbon paste electrodes (I and II) based on 5,5’-(propane-1,3-diylbis(sulfanediyl))bis(3-benzyl-4H-1,2,4-triazol-4-amine) (electrode I, A-ionophore) and 5,5’-(butane-1,4-diyl-bis(s...The new ferrous-selective modified carbon paste electrodes (I and II) based on 5,5’-(propane-1,3-diylbis(sulfanediyl))bis(3-benzyl-4H-1,2,4-triazol-4-amine) (electrode I, A-ionophore) and 5,5’-(butane-1,4-diyl-bis(sulfane- diyl))bis(3-benzyl-4H-1,2,4-triazol-4-amine) (electrode II, B-ionophore) as ionophores are described. These electrodes exhibit Nernstian slopes of 30.2 ± 0.5 and 29.1 ± 0.5 mV·decade-1, linear range of 1.0 × 10-7 - 1.0 × 10-2 mol·L-1 Fe(II) ion and detection limit of 1.0 × 10-7 mol·L-1 Fe(II) ion for electrode (I) and electrode (II), respectively. Both electrodes (I and II) have a fast response time of about 15 sand can be used for at least 3 months. The two electrodes revealed a good selectivity for Fe(II) over a wide variety of other metal ions and could be used in the pH range of 1.8 - 3.0 without any divergence in potential. The proposed sensors were successfully applied for the determination of Fe(II) ion in different real samples.展开更多
The acrosome reaction of sperm was induced by calcium ionophore A 23187.The letionship between some ions and acrosome reaction by removing Na+ from the medium.or by adding angtagonist of K+.TEA chloride,or antagonist ...The acrosome reaction of sperm was induced by calcium ionophore A 23187.The letionship between some ions and acrosome reaction by removing Na+ from the medium.or by adding angtagonist of K+.TEA chloride,or antagonist of Ca++,verapamil,or anlagonist of Na+-K+-ATPase,acetyl strophanthithidin is studied.The results show that Na+,H,Ca++ and Na+ pump are necessary.for acrosome reaclion of human sperm.The Ca++ might not enter the sperms through the channel of Ca++.展开更多
Total or near-total fertilization failure after intracytoplasmic sperm injection (ICSl) is a rare event, but it occurs repeatedly because of sperm defects in activating oocyte. The case presents a successful pregnan...Total or near-total fertilization failure after intracytoplasmic sperm injection (ICSl) is a rare event, but it occurs repeatedly because of sperm defects in activating oocyte. The case presents a successful pregnancy and live birth after calcium ionophore A23187 (A23187) activation on one-day-old unfertilized oocytes in a patient whose husband suffered oligoasthenoteratozoospermia, and who had experienced repeated near-total fertilization failure after ICSI. In the second ICSI cycle, only one oocyte was fertilized while nine were unfertilized. Oocyte activation with A23187 were performed on the one-day-old unfertilized oocytes after ICSI and resulted in fertilization and embryo transfer. A clinical pregnancy was achieved and a healthy baby was born. To our knowledge, this is the first reported case of a healthy birth after oocyte activation on the one-day-old unfertilized oocyte. This indicates that "rescue oocyte activation" on one-day-old unfertilized oocytes after ICSI may be helpful for preventing total or near-total fertilization failure after ICSI.展开更多
基金supported by the Chinese Academy of Sciences(No.KZCX2-YW-410)the National Natural Science Foundation of China(No.40776058)+1 种基金the National 863 High Technology Project of the Ministry of Science and Technology of China(No.2007AA09Z103)the Outstanding Youth Natural Science Foundation of Shandong Province(No.JQ200814)
文摘Four 20-membered N_2S_4-monoazathiacrown ethers have been synthesized and explored as neutral ionophores for Ag^+-selective electrodes.Potentiometric responses reveal that the flexibility of the ligands has great effect on the selectivity and sensitivity to Ag^+ ions.The electrode based on ionophore 9,10,20,25-tetrahydro-5H,12H-tribenzo[b,n,r][1,7,10,16,4,13]tetrathiadiaza cycloicosine 6,13-(7H,14H)-dione(C) with 2-nitrophenyl octyl ether(o-NPOE) as solvent in a poly(vinyl chloride)(PVC) membrane matrix sho...
文摘Timing of vegetal-endodermal cell determination in amphioxus embryos remains uncertain. We tentatively testal effects of A23187, the calcium ionophore, on the deveopment of vegetal blastomeres isolated at the 16-cell stage. It was found that when vegetal blastomres committed to endodermwere treated with A23187 prior to gastrulation, they were transformed into ectodermal cells as evidenced by the cell morphology and function characteristic of epidermis. Howver, the developmental fate of the sam blastomeres untreated or treated with DMSO at the same stage or of those treated with A23187 after gastrulation remained unchanged. Thus, vegetal-endodermal cells in amphioxus embryos are not irreversibly deermined before the gastrula stage, and artificial incarease in intracelluar Ca2+ concentration can induce transdetermination of the predetermined endodermal cells into ectodermal cells.
文摘A new type of di benzoyl tartaric acid selective electr ode has been developed. Three double\| arm calixarene derivatives were emp loyed as the neutral ionophores. The poly(vinyl chloride) membrane electrode containing an amide derivative of ca lixarene as the neutral carrier an d a dibutyl phthalate as the plastici zer exhibited the highest sensitivity for dibenzoyl tartaric acid. The slop e of linear portion was 27.8 mV per c oncertration decade. The electrode has a fast response and a long lifetime .
文摘A thiocyanate ion selective poly(aniline) solid contact electrode based on manganese complex of N,N’-bis-(4-phenylazosalicylidene)-o-phenylene diamine ionophore was successfully developed. The electrode exhibits a good linear response of 58.1 mV/decade (at 20?C ± 0.2?C, r2 = 0.998) with in the concentration range of 1 × 10–1.0 ~ 1 × 10–5.8 M thiocyanate solution. The composition of this electrode was: ionophore 0.040, polyvinylchloride 0.300, dibutylphthalate 0.660 (mass). This dibutylphthalate plasticizer provides the best response characteristics. The electrode shows good selectivity for thiocyanate ion in comparison with any other anions and is suitable for use with aqueous solutions of pH 4.0 ~ 6.0. The standard deviations of the measured emf difference were ±1.70 and ±2.01 mV for thiocyanate sample solutions of 1.0 × 10–2 M and 1.0 × 10–3 M, respectively. The stabilization time was less than 170 sec. and response time was less than 17 sec.
文摘The release of mediators from mast cells is a model for cell secretion and is an in-vitro index for immediate hypersensitivity reactions. Calcium influx is generally accepted to be the primary biochemicalevent in mast cell activation.We studied the effect of the calcium ionophore A 23187 and calcium channelblockers,nifedipine and verapamil, in triggering the activation of rat peritoneal mast cells.At suitableconcentration nifedipine and verapamil have had the inhibition effect in the IgE-dependent roaction.
文摘Novel solid-contact perchlorate sensors based on cobalt phthalocyanine-C-monocarboxylic acid (I), and cobalt phthalocyanine-C,C,C,C-tetracarboxylic acid (II) as free ionophores and covalently attached to polyacryla- mide (PAA)—ionophores III and IV, respectively were prepared. The all solid-state sensors were constructed by the application of a thin film of a polymer cocktail containing a phthalocyanine ionophore and cetyltrimethylammonium bromide (CTMAB) as a lipophilic cationic additive onto a gold electrode precoated with the conducting polymer poly (3,4-ethylenedioxythiophene) (PEDOT) as an ion and electron transducer. The sensor with 10.3% of ionophore (III) covalently attached to plasticizer-free poly (butyl methacrylate-co-do- decyl methacrylate) (PBDA) exhibited a good selectivity for perchlorate and discriminated many ions, in- cluding F–, Cl–, Br–, I–, SCN–, , S2– and . The covalent attachment of the ionophore to the polymer resulted in a near-Nernstian anionic slope of –62.3 mV/decade whereas a super-Nernstian slope of –79.9 mV/ decade was obtained for the free ionophore. The sensor covered a linear concentration range of 5 × 10–9 - 1 × 10–2 mol?L–1 with a lower detection limit (LDL) of 1 × 10–9 mol?L–1 and gave a stable response over a pH range of 4 - 10.5. The all-solid state sensors were utilized for the selective flow injection potentiometric determination of perchlorate in natural water and human urine samples in the nanomolar concentration range.
文摘<strong>Objectives:</strong><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> Evaluate the effect of artificial oocyte activation (AOA) using calcium ionophore (A23187) on the rate of fertilization and cleavage of embryos in surgically retrieved sperm of patients with non-obstructive azoospermia undergoing intracytoplasmic sperm injection (ICSI).</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">Study design: </span></b></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">This study was conducted on 60 infertile couples undergoing ICSI cycles as a randomized controlled parallel group’s experimental study in a private IVF center in Egypt from January 2018 to July 2019. ICSI cycles were divided into two g</span><span style="font-family:Verdana;">roups: Group A: includes 30 ICSI patients with surgically retrieved sperms of non-obstructive azoospermia treated with calcium ionophore (A23187). Group C/Control: includes 30 ICSI patients with surgically retrieved sperms of non-obstructive azoospermia non-treated with calcium ionophore (A23187)</span><span style="font-family:Verdana;">. </span><b><span style="font-family:Verdana;">Results: </span></b><span style="font-family:Verdana;">There was no statistical difference between both groups regarding the fertilization rate (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> = 0.853). There was no statistical difference between them regarding implantation rate (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> = 0.237). The percentage of Class A embryos in the ca ionophore group was 81.7%, while it was 82.8% in the control group. There was insignificant difference between them (</span><i><span style="font-family:Verdana;">p</span></i><span style="font-family:Verdana;"> = 0.782). There was no statistical significant difference between the two groups regarding the clinical pregnancy rate</span></span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">,</span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;"> it was (56.7%) in the ca ionophore group while it was (53.3%) in the control group.</span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><b><span style="font-family:Verdana;">Conclusion: </span></b></span></span><span><span><span style="font-family:""><span style="font-family:Verdana;">AOA by Ca</span><sup><span style="font-family:Verdana;">2++</span></sup></span></span></span><span><span><span style="font-family:""> </span></span></span><span style="font-family:Verdana;"><span style="font-family:Verdana;"><span style="font-family:Verdana;">ionophore didn’t improve the outcome of ICSI cycle in cases of non obstructive azoospermia in terms of fertilization, implantation and pregnancy rate.</span></span></span>
基金supported by grants from the National Key R&D Program of China(No.2017YFA0700500)the National Natural Science Foundation of China(No.21635004)。
文摘Ionophore can prominently improve the ion permeability of cell membrane and disrupt cellular ion homeostasis.Most studies regarding ionophore facilitating ion transmembrane transport focus on artificial liquid-liquid interfaces,which have large difference from the actual environment of cell membrane.Here,we construct a supported lipid bilayeron a gold nanoparticles film modified ZnSe prism as an appropriate model of cell membrane to investigate the dynamic of the ion transport facilitated by ionophore using surface enhanced infrared absorption spectroscopy(SEIRAS).We find that the ion transmembrane transport consists of two steps:The ion transmembrane transport starts with the association/disassociation between ion and ionophore at the edge of lipid bilayer;The second step is the transfer of ion-ionophore complex across lipid bilayer.Our results show that the complex transfer across the lipid bilayer is the rate determining step.
文摘We use the extended gate field effect transistor (EGFET)as the structure of the chlorine ion sensor,and the chlorine ion ionophores (ETH9033 and TDDMAC1)are incorporated into solvent polymeric membrane (PVC/DOS),then the chlorine ion selective membrane is formed on the sensing window,and the fabrication of the EGFET chlorine ion sensing device is completed.The surface potential on the sensing membrane of the EGFET chlorine ion sensing device will be changed in the different chlorine ion concentration solutions,then changes further gate voltage and drain current to detect chlorine ion concentration.We will study non-ideal effects such as temperature,hysteresis and drift effects for the EGFET chlorine ion sensing device in this paper,these researches will help us to improve the sensing characteristics of the EGFET chlorine ion sensing device.
文摘A new chemically modified carbon paste electrode for cesium(I) ion determination based on potassium zinc hexacyan-oferrate (PZHCF) as an ionophore was prepared. The electrode exhibits a Nernstian response for Cs(I) ions over a wide concentration range from 1 × 10-6 to 1 × 10-1 mol·L-1 with a slope of 58 ± 0.5 mV·decade-1. It has a response time of about 35 s and can be used for a period of 3 months with good reproducibility. Detection limit obtained in the optimal conditions was 3 × 10-7 mol·L-1. The potentiometric response is independent of the pH of the solution in the pH range 4.0 - 8.0. The electrode possesses the advantages of low resistance, fast response over a variety of other cations. The proposed electrode is applied as a sensor for the determination of Cs(I) ion concentration in different samples solutions. The results showed a good correlation with the data obtained by atomic absorption spectrometric method.
基金This work was supported by the Natural Science Foundation of Hubei Province,China
文摘A modified method of preparing crown functionalized linear polysiloxane has been described. 4'-Allyldibenzo-18-crown-6 was subjected to hydrosilylation with methyldichlorosilane, followed by polycondensation with silanol-terminated polydimethylsiloxane to give the title crown functionalized linear polysiloxane. The transport properties of sodium, potassium, and ammonium salt through a bulk liquid membrane system using the new type of crown functionalized linear polysiloxane as a carrier were investigated. It is worthy to point out that the carrier can be used repeatedly at least six runs with no apparent change in the transport rate of potassium ion.
文摘Objective: To study the role of apoptosis in tumor cell of malignant glioma death following treatment with hyperthermia and calcium ionophore. Methods: The apoptosis induced by hyperthermia and calcium ionophore, A23187, in human glioblastoma cell line TJ905 and murine glioblastoma G422 was evaluated by characteristic findings in DNA agarose gel electrophresis, ultrastructural examination and flow cytometric analysis. Results: Apoptosis could be induced by moderate hyperthermia, but not by mild hyperthermia, calcium ionophore enhanced significantly the effect of mild hyperthermia on the induction of apoptosis. Conclusion: This result indicates that apoptotic cell death is one of the mechanisms of hyperthermic therapy for malignant glioma and taking measures to increase the cytolic calcium may enhance the effect of hyperthermia.
文摘Ionophores consist of molecules which surround and carry positive metal ions and other ions through biological membranes. One classe of ionophores which we have been developing contains dipeptides which are encouraged to become part of a ring because of possible hydrogen bond formation between the 2-hydroxy on the phenyl group and carboxyl group (COOH) of the final amide proline. Formation of a ring should increase the complexation ability of the ionophore. We report that the synthesis of N-(2-hydroxyl-1-phenoxyacetyl) prolyproline(1), a novel ionophore is prepared from activated 2-acetoxy phenoxyacetic acid (3a) and the appropriate dipeptide ester using coupling methods such as dicyclohexyl carbodiimide with hydroxyben-ztriazole or carbonyl diimidazole.
文摘The release of mediators from mast cells is a model for cell secretion and is an in-vitro index for immediate hypersensitivity reactions. Calcium influx is generally accepted to be the primary biochemicalevent in mast cell activation.We studied the effect of the calcium ionophore A 23187 and calcium channelblockers,nifedipine and verapamil, in triggering the activation of rat peritoneal mast cells.At suitableconcentration nifedipine and verapamil have had the inhibition effect in the IgE-dependent roaction.
文摘Beef cattle producers in the North America have a variety of production and marketing options and must choose the best production system for their situation. This review describes considerations involved in choosing between feeding cattle conventionally versus feeding them in programs that prohibit the use of certain technologies. Data from peer-reviewed journals, extension publications, nutritional consultants, governmental organizations, and feed companies were used to construct this review. Most cattle in North America are fed in conventional production systems. Conventional beef production systems typically use steroidal implants, ionophores, and beta-adrenergic agonists to improve animal productivity;as well as feed grade and injectable antimicrobials to control, treat or prevent disease and improve animal health. These technologies have been shown to lower the cost of production, allowing for beef to be competitive in the global protein market. Some consumers have expressed a preference for beef produced without these technologies. These “All-natural” (AN) cattle may bring a premium price in the market. The economic impact of differing productions systems can be described in relation to 1) cost of production, 2) operating costs of the feedlot, 3) price paid for feeder calves, and 4) price received for fed cattle. Conventional production provides the most favorable outcome for factors 1, 2, and 3, while AN production provides the most favorable outcome for item 4. There are also industry wide and societal aspects related to differing beef production systems related to health and safety of beef, land use, and cost of production allowing for a greater share of the global protein market. Technologies used in conventional production are critical tools to North American beef production. Differences in efficiencies between each type of non-conventional production systems must be re-captured in added premiums when cattle are marketed and sold. Premiums for AN cattle are enticing, but the true differences in the cost of production between the AN and conventional cattle must be evaluated in order for a producer to make the correct decision for their operation.
文摘The new ferrous-selective modified carbon paste electrodes (I and II) based on 5,5’-(propane-1,3-diylbis(sulfanediyl))bis(3-benzyl-4H-1,2,4-triazol-4-amine) (electrode I, A-ionophore) and 5,5’-(butane-1,4-diyl-bis(sulfane- diyl))bis(3-benzyl-4H-1,2,4-triazol-4-amine) (electrode II, B-ionophore) as ionophores are described. These electrodes exhibit Nernstian slopes of 30.2 ± 0.5 and 29.1 ± 0.5 mV·decade-1, linear range of 1.0 × 10-7 - 1.0 × 10-2 mol·L-1 Fe(II) ion and detection limit of 1.0 × 10-7 mol·L-1 Fe(II) ion for electrode (I) and electrode (II), respectively. Both electrodes (I and II) have a fast response time of about 15 sand can be used for at least 3 months. The two electrodes revealed a good selectivity for Fe(II) over a wide variety of other metal ions and could be used in the pH range of 1.8 - 3.0 without any divergence in potential. The proposed sensors were successfully applied for the determination of Fe(II) ion in different real samples.
文摘The acrosome reaction of sperm was induced by calcium ionophore A 23187.The letionship between some ions and acrosome reaction by removing Na+ from the medium.or by adding angtagonist of K+.TEA chloride,or antagonist of Ca++,verapamil,or anlagonist of Na+-K+-ATPase,acetyl strophanthithidin is studied.The results show that Na+,H,Ca++ and Na+ pump are necessary.for acrosome reaclion of human sperm.The Ca++ might not enter the sperms through the channel of Ca++.
文摘Total or near-total fertilization failure after intracytoplasmic sperm injection (ICSl) is a rare event, but it occurs repeatedly because of sperm defects in activating oocyte. The case presents a successful pregnancy and live birth after calcium ionophore A23187 (A23187) activation on one-day-old unfertilized oocytes in a patient whose husband suffered oligoasthenoteratozoospermia, and who had experienced repeated near-total fertilization failure after ICSI. In the second ICSI cycle, only one oocyte was fertilized while nine were unfertilized. Oocyte activation with A23187 were performed on the one-day-old unfertilized oocytes after ICSI and resulted in fertilization and embryo transfer. A clinical pregnancy was achieved and a healthy baby was born. To our knowledge, this is the first reported case of a healthy birth after oocyte activation on the one-day-old unfertilized oocyte. This indicates that "rescue oocyte activation" on one-day-old unfertilized oocytes after ICSI may be helpful for preventing total or near-total fertilization failure after ICSI.