To investigate the chemical constituents of the roots ofPolygala sibirica L. The separation and purification were performed by solvent extraction and repeated chromatography with silica gel, Sephadex LH-20, ODS column...To investigate the chemical constituents of the roots ofPolygala sibirica L. The separation and purification were performed by solvent extraction and repeated chromatography with silica gel, Sephadex LH-20, ODS columns, and semiprep. HPLC. The structures were elucidated by spectral analysis. Twelve known compounds were isolated and identified as tenuifoliside A （1）, tenuifoliside B （2）, glomeratose A （3）, 3＇,6-disinapoyl sucrose （4）, sibiricose A5 （5）, sibiricose A6 （6）, sibiricose A1 （7）, sibiricose A2 （8）, polygalatenoside E （9）, 1-O-L-arabinopyranosyl-O-（6→1）-β-D-glucopyranosyl-salicylate （10）, canthoside A （11）, and methyl- 3,4,5-trimethoxycinnamate （12）. Compound 11 was obtained from genus Polygala for the first time, and compounds 2, 9, 10 and 12 were isolated from this plant for the first time.展开更多
Siberian apricot(Prunus sibirica L.)is a traditional nut tree species in East Asia and Siberia and is a possible contribution to healthy diets.However,it has attracted little research attention and information on the ...Siberian apricot(Prunus sibirica L.)is a traditional nut tree species in East Asia and Siberia and is a possible contribution to healthy diets.However,it has attracted little research attention and information on the nutritional value of its kernel is limited.In this study,the profile,nutritional value and variation of amino acids were investigated in eight provenances.The kernels contained29×10-2 g/g protein and were rich in glutamic acid(26.5%),aspartic acid(11.3%)and arginine(10.1%).They showed higher essential amino acids(EAA)than similar protein values for almonds.The variation coefficients of amino acids ranged from 3.8 to 43.7%,and the levels of seven amino acids were significantly different among the eight provenances.The proportion of essential amino acids to total amino acids and amino acid score were also quite different.Protein was negatively correlated with some amino acids and protein quality values.In conclusion,there were two superior provenances(Wanjiagou and Horinger County)with high EAA contents and protein quality,and could be used in the large-scale development of this species.展开更多
A new iridoid glycoside,versibirioside（1）,and a known iridoid glycoside,verbaspinoside（2）,were isolated from the whole plant of Veronica sibirica L.Their structures were elucidated by spectroscopic methods,includi...A new iridoid glycoside,versibirioside（1）,and a known iridoid glycoside,verbaspinoside（2）,were isolated from the whole plant of Veronica sibirica L.Their structures were elucidated by spectroscopic methods,including 2D-NMR spectra.展开更多
Quantitative real-time PCR(qPCR)is an effective and widely used method to analyze expression patterns of target genes.Selection of stable reference genes is a prerequisite for accurate normalization of target gene exp...Quantitative real-time PCR(qPCR)is an effective and widely used method to analyze expression patterns of target genes.Selection of stable reference genes is a prerequisite for accurate normalization of target gene expression by qRT-PCR.In Iris germanica L.,no studies have yet been published regarding the evaluation of potential reference genes.In this study,nine candidate reference genes were assessed at different flower developmental stages and in different tissues by four different algorithms(GeNorm,NormFinder,BestKeeper,and RefFinder).The results revealed that ACT11(Actin 11)and EF1α(Elongation factor 1 alpha)were the most stable reference genes in different tissues,whereas TUA(Tubulin alpha)and UBC9(Ubiquitin-protein ligase 9)were the most stable ones in different flower developmental stages.UBC9 and ACT11 were the most stable reference genes in all of the tested samples,while the SAMDC(S-Adenosylmethionine decarboxylase)showed the least stability.Finally,to validate the suitability of the selected reference genes,the relative expression level of IgTPS(beta-caryophyllene synthase)was assessed and highlighted the importance of suitable reference gene selection.This work constitutes the first systematic evaluation of potential reference genes in I.germanica and provides guidelines for future research on gene function and molecular mechanisms on I.germanica and related species.展开更多
基金Program for Changjiang Scholar and Innovative Team in University(Grant No.985-2-063-112)Program for New Century Excellent Talents in University(Grant No.985-2-102-113).
文摘To investigate the chemical constituents of the roots ofPolygala sibirica L. The separation and purification were performed by solvent extraction and repeated chromatography with silica gel, Sephadex LH-20, ODS columns, and semiprep. HPLC. The structures were elucidated by spectral analysis. Twelve known compounds were isolated and identified as tenuifoliside A （1）, tenuifoliside B （2）, glomeratose A （3）, 3＇,6-disinapoyl sucrose （4）, sibiricose A5 （5）, sibiricose A6 （6）, sibiricose A1 （7）, sibiricose A2 （8）, polygalatenoside E （9）, 1-O-L-arabinopyranosyl-O-（6→1）-β-D-glucopyranosyl-salicylate （10）, canthoside A （11）, and methyl- 3,4,5-trimethoxycinnamate （12）. Compound 11 was obtained from genus Polygala for the first time, and compounds 2, 9, 10 and 12 were isolated from this plant for the first time.
基金This study is supported by Fundamental Research Funds for the Central Non-profit Research Institution of Chinese Academy of Forestry (CAFYBB2017ZA004-4)
文摘Siberian apricot(Prunus sibirica L.)is a traditional nut tree species in East Asia and Siberia and is a possible contribution to healthy diets.However,it has attracted little research attention and information on the nutritional value of its kernel is limited.In this study,the profile,nutritional value and variation of amino acids were investigated in eight provenances.The kernels contained29×10-2 g/g protein and were rich in glutamic acid(26.5%),aspartic acid(11.3%)and arginine(10.1%).They showed higher essential amino acids(EAA)than similar protein values for almonds.The variation coefficients of amino acids ranged from 3.8 to 43.7%,and the levels of seven amino acids were significantly different among the eight provenances.The proportion of essential amino acids to total amino acids and amino acid score were also quite different.Protein was negatively correlated with some amino acids and protein quality values.In conclusion,there were two superior provenances(Wanjiagou and Horinger County)with high EAA contents and protein quality,and could be used in the large-scale development of this species.
文摘A new iridoid glycoside,versibirioside（1）,and a known iridoid glycoside,verbaspinoside（2）,were isolated from the whole plant of Veronica sibirica L.Their structures were elucidated by spectroscopic methods,including 2D-NMR spectra.
基金The work was supported by the National Natural Science Foundation of China(31801901)the Natural Science Foundation of Jiangsu(BK20180314)+1 种基金the Foundation of Key Laboratory of Landscaping(KF201901),Ministry of Agriculture and Rural Affairs,Chinathe Jiangsu Key Laboratory for the Research and Utilization of Plant Resources(JSPKLB201814).
文摘Quantitative real-time PCR(qPCR)is an effective and widely used method to analyze expression patterns of target genes.Selection of stable reference genes is a prerequisite for accurate normalization of target gene expression by qRT-PCR.In Iris germanica L.,no studies have yet been published regarding the evaluation of potential reference genes.In this study,nine candidate reference genes were assessed at different flower developmental stages and in different tissues by four different algorithms(GeNorm,NormFinder,BestKeeper,and RefFinder).The results revealed that ACT11(Actin 11)and EF1α(Elongation factor 1 alpha)were the most stable reference genes in different tissues,whereas TUA(Tubulin alpha)and UBC9(Ubiquitin-protein ligase 9)were the most stable ones in different flower developmental stages.UBC9 and ACT11 were the most stable reference genes in all of the tested samples,while the SAMDC(S-Adenosylmethionine decarboxylase)showed the least stability.Finally,to validate the suitability of the selected reference genes,the relative expression level of IgTPS(beta-caryophyllene synthase)was assessed and highlighted the importance of suitable reference gene selection.This work constitutes the first systematic evaluation of potential reference genes in I.germanica and provides guidelines for future research on gene function and molecular mechanisms on I.germanica and related species.
