Seeds of japonica rice (cv. Zhenuo 2) at twodifferent physiological states (dry seeds withwater content 13% and wet seeds soaked inthe water for 36 h) were irradiated by COlaser in four different power-densities. Thes...Seeds of japonica rice (cv. Zhenuo 2) at twodifferent physiological states (dry seeds withwater content 13% and wet seeds soaked inthe water for 36 h) were irradiated by COlaser in four different power-densities. Theseeds irradiated by 200GY ofCo γ rays andno radiated seeds were used as the controls.Results showed that the biological effects展开更多
[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N＋ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds w...[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N＋ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds were selected respectively,and then the total RNA of seedlings after 96 h of germination was extracted at 30 ℃.Agilent gene chip was used to screen the differentially expressed genes of two groups of rice seedlings.The chip contained 510 miRNA probes;and about two times of expression differences between two samples were considered as the threshold range.[Result]14 miRNA molecules showed significant expression differences between the irradiated group and the control group,and all of them were decreased.[Conclusion]The miRNAs showed expression differences between irradiated group and the control group,which might regulate the expression of certain genes to respond to ion irradiation,thus producing physiological,biochemical and phenotypic differences.展开更多
Eight lines of temperature-responsive leaf colormutants induced by applying 300 Gy Gamma-ray irradiation to Thermo-sensitive genic malesterile line 2177s,were obtained through con-tinuous selection in seven generation...Eight lines of temperature-responsive leaf colormutants induced by applying 300 Gy Gamma-ray irradiation to Thermo-sensitive genic malesterile line 2177s,were obtained through con-tinuous selection in seven generations..Theleaves of these lines started to become greenafter the fourth leaf extension,and except展开更多
文摘Seeds of japonica rice (cv. Zhenuo 2) at twodifferent physiological states (dry seeds withwater content 13% and wet seeds soaked inthe water for 36 h) were irradiated by COlaser in four different power-densities. Theseeds irradiated by 200GY ofCo γ rays andno radiated seeds were used as the controls.Results showed that the biological effects
基金Supported by National Natural Science Foundation of China(308200204)Natural Science Research Program in Education Department of Henan Province(2009B180019)~~
文摘[Objective] The aim of this study was to explore the expression differences of miRNA response to low energy N＋ beam radiation in rice.[Method]Three groups of ion beam irradiation rice seeds and untreated rice seeds were selected respectively,and then the total RNA of seedlings after 96 h of germination was extracted at 30 ℃.Agilent gene chip was used to screen the differentially expressed genes of two groups of rice seedlings.The chip contained 510 miRNA probes;and about two times of expression differences between two samples were considered as the threshold range.[Result]14 miRNA molecules showed significant expression differences between the irradiated group and the control group,and all of them were decreased.[Conclusion]The miRNAs showed expression differences between irradiated group and the control group,which might regulate the expression of certain genes to respond to ion irradiation,thus producing physiological,biochemical and phenotypic differences.
文摘Eight lines of temperature-responsive leaf colormutants induced by applying 300 Gy Gamma-ray irradiation to Thermo-sensitive genic malesterile line 2177s,were obtained through con-tinuous selection in seven generations..Theleaves of these lines started to become greenafter the fourth leaf extension,and except
