Isolation and Purification of Isoaloeresin D and Aloin from Aloe vera by High-speed Counter-current Chromatography

Objective To develop an efficient method to isolate and purify the main components isoaloeresin D and aloin from Aloe vera for its industrial production.Methods High-speed counter-current chromatography was used to isolate isoaloeresin D and aloin in a one-step separation from dried crude extract of A.vera.The biphasic solvent system composed of hexane-ethyl acetate-acetone-water(0.2:5:1.5:5) was used at a flow rate of 1.0 mL/min,while the lipophilic phase was selected as the mobile phase and the apparatus was rotated at 840 r/min.The effluent was detected at 254 nm.Results Isoaloeresin D(53.1 mg) and aloin(106.9 mg) were separated from the crude extract(384.7 mg) with the purities of 98.6% and 99.5%,respectively.Conclusion HSCCC is a powerful technique for isolation and separation of chemical composition from aloe.

芦荟苷A、B以及异芦荟色苷D的同时分离纯化

首次采用反相中压制备色谱从芦荟中一次分离制备得到芦荟苷A、B以及异芦荟色苷D。以库拉索芦荟丙酮粗提物为原料,采用中压制备色谱系统:SCO色谱柱(40 cm×26 cm,30～50μm),流动相甲醇-0.5%乙酸水(33∶67,V/V),流速20 mL/min,等度洗脱方式,柱温室温,检测波长254 nm,收集波长356 nm对芦荟样品进行分离制备,得到三种化合物单体。经高效液相色谱、紫外、红外、质谱及核磁共振等方法分析表明所得到的三种化合物分别是异芦荟色苷D、芦荟苷A和芦荟苷B,其纯度分别达到了98.0%,96.0%和98.9%。该方法简便,产品质量高,一次制备可以得到多种单体,为芦荟成分的测定与药理活性的研究提供了条件。

高效毛细管电泳法分析库拉索芦荟中的多种成分

目的建立分离芦荟苷同分异构体及同时测定不同库拉索芦荟样品中芦荟苷、异芦荟色苷D、芦荟大黄素含量的毛细管区带电泳法(CZE),并对各种影响电泳分离的因素进行了探讨。方法以芦丁为内标,采用未涂层弹性熔融石英毛细管柱(57 cm×75μm,有效长度50 cm),以20 mmol·L-1硼砂+2 mmol·L-1β-环糊精(pH=9.65)为背景电解质溶液,分离电压为18 kV,重力进样5 s(高度15 cm),检测波长365 nm。结果芦荟苷的一对同分异构体能够达到良好的分离,以芦丁为内标,库拉索芦荟样品中的芦荟苷、异芦荟色苷D、芦荟大黄素3种成分在7 min内得到良好的分离测定。结论此方法简便,快速,成本低,可用于芦荟样品的质量控制。