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Complete genome sequence analysis of two Citrus tatter leaf virus(CTLV) isolates from China
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作者 SONG Zhen LI Zhong-an +1 位作者 LIU Ke-hong ZHOU Chang-yong 《Journal of Integrative Agriculture》 SCIE CAS CSCD 2015年第5期984-987,共4页
In order to understand molecular characterization of Citrus tatter leaf virus(CTLV) isolated from China,full-length cDNAs of CTLV-MTH and CTLV-XHC from Citrus reticulata and Citrus sinensis were cloned and sequenced... In order to understand molecular characterization of Citrus tatter leaf virus(CTLV) isolated from China,full-length cDNAs of CTLV-MTH and CTLV-XHC from Citrus reticulata and Citrus sinensis were cloned and sequenced based on whole-genome amplification by RT-PCR.The complete nucleotide sequences of CTLV-MTH and CTLV-XHC were determined to be 6497 nucleotides in length and shared 79.9-91.0%and 78.8-98.0%nucleotide sequence identity,respectively,with other Apple stem grooving virus(ASGV) or CTLV strains available in GenBank.Unexpectedly,CTLV-MTH showed the highest nucleotide sequence identity(91%) with an apple isolate of ASGV,followed by 86.5%with ASGV-HH and 85.7%with ASGV-CHN.Furthermore,CTLV-MTH and three ASGV strains were grouped to a separate cluster in the phylogenetic tree,suggesting it has a closer relationship to ASGV than to CTLV.Therefore,it can be concluded roughly that CTLV may be not a distinct strains of ASGV.We proposed that Citrus tatter leaf virus should be renamed Apple stem grooving virus. 展开更多
关键词 Citrus isolates Apple apple citrus sequenced length phylogenetic cloned orange
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Cloning and seqencing of partial gene from SENV isolate in China
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《中国输血杂志》 CAS CSCD 2001年第S1期419-,共1页
关键词 SENV Cloning and seqencing of partial gene from SENV isolate in China GENE
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Isolating cDNA clones from rice induced by Magnaporthe grisea using PCR based differential screening method
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作者 DONG Haitao, DONG Jixin, WU Yuliang, HE Zuhua,and Li Debao,Biotechnology Institute,ZheJiang Agri Univ, Hangzhou 310029, China 《Chinese Rice Research Newsletter》 1998年第3期1-2,共2页
The differential hybridization technique hasbeen widely used to identify genes that are dif-ferentially expressed.However,this approachhas several drawbacks.First,the screeningprocedures are rather labor-intensive and... The differential hybridization technique hasbeen widely used to identify genes that are dif-ferentially expressed.However,this approachhas several drawbacks.First,the screeningprocedures are rather labor-intensive and time-consuming.Second,the amount of phageDNAs transferred onto the two filters may notbe equivalent,which leads to an inaccurate se-lection of a positive clone.Third,isolation ofphage DNA is slow and cumbersome.Here,aPCR based differential screening method that 展开更多
关键词 cDNA PCR Isolating cDNA clones from rice induced by Magnaporthe grisea using PCR based differential screening method
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Isolation and cloning of the gene encoding high affinity phosphate transporter in rice 被引量:2
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作者 MING Feng LIANG Bing YE Mingming,and SHEN Daling,Inst of Genetics,Fudan Univ,Shanghai 200433,China 《Chinese Rice Research Newsletter》 2002年第1期4-5,共2页
High affinity phosphate transporterplays an important role in plantadapting to low phosphorus. Isolationof genes coding this kind of proteinhas attracted worldwide scholars toaccomplish. We aimed to isolate thegene an... High affinity phosphate transporterplays an important role in plantadapting to low phosphorus. Isolationof genes coding this kind of proteinhas attracted worldwide scholars toaccomplish. We aimed to isolate thegene and transfer it to target plants 展开更多
关键词 CGG CGA GCT CAC CCC Isolation and cloning of the gene encoding high affinity phosphate transporter in rice high gene
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