Exogenous angiotensin Ⅰ(ANG Ⅰ) was degraded to mainly des-Asp-ANGⅠinstead of ANG Ⅱ in the hypothalarnic homogenate of the Sprague Dawley (SD). Wistar Kyoto (WKY), left renal artery stenosed hypertensive SD (LRAS),...Exogenous angiotensin Ⅰ(ANG Ⅰ) was degraded to mainly des-Asp-ANGⅠinstead of ANG Ⅱ in the hypothalarnic homogenate of the Sprague Dawley (SD). Wistar Kyoto (WKY), left renal artery stenosed hypertensive SD (LRAS), deoxycorticosterone acetale/salt - induced hypertensive SD (DOCA-sall) and spontaneously hypertensive rats (SHR). In the sanie honiogenate, ANG Ⅱ was degraded to ANG Ⅲ, and ANG Ⅲ remained unchanged during 15 min incubation. However, all the homapenates were able to catalyse hippuryl-L-histiayl'-L-leucine to hippuric acid and the catalysis was completely inhibited by 3 PM captorpil. The data showed that angiotensin converting enzyrne presenl in the hypothalamus when extracted by the normal laboratory procedures was not able to hydrolyse ANG Ⅰ to ANG Ⅱ. In addition, the aminopeptidase that degraded ANG Ⅰ to des-Asp-ANG Ⅰ was not inhibited by amastatin, bestatin and EDTA, indicating that it is not aminopeptidase A or B. The formation of hippuric acid was significantly higher in the homapenate of the LRAS whilst the SHR and DOCA-salt showed a significant higher rate of des-Asp-ANG Ⅰ formation. These findings are the first dernonsiration of the formation of des-Asp-ANG Ⅰ as the major product of ANG Ⅰ degradation in the hypothalamic homagenate of the rat. The data also show that in two models of low renin hypertensive rats the formation of nanopeptide is significantly elevated.展开更多
Objective To detect the A/C1165 polymorphism of angiotensin Ⅱ type Ⅰ receptor (AT1-R)gene in essential hypertensive elderly. Methods The A/C1166 polymorphism of AT1-R gene was assessed by polymerase chain reaction-r...Objective To detect the A/C1165 polymorphism of angiotensin Ⅱ type Ⅰ receptor (AT1-R)gene in essential hypertensive elderly. Methods The A/C1166 polymorphism of AT1-R gene was assessed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in a case-control study of 87 essential hypertensive elders (EH) and 55 normolensive elders (NT). Results The genotype frequencies of AA, AC, CC were 0 .805 , 0.161, 0 .034 in EH group and 0 .927 ,0 .073 ,0 .000 in NT group respectively. The frequency of C61166 allele was higher in EH group (0.115) than in NT group (0 .036 )(P<0 .05 ). Conclusion The resultsindicate that A/C1166 polymorphism of AT1-R gene may be associated with essential hypertension in elderly.展开更多
文摘Exogenous angiotensin Ⅰ(ANG Ⅰ) was degraded to mainly des-Asp-ANGⅠinstead of ANG Ⅱ in the hypothalarnic homogenate of the Sprague Dawley (SD). Wistar Kyoto (WKY), left renal artery stenosed hypertensive SD (LRAS), deoxycorticosterone acetale/salt - induced hypertensive SD (DOCA-sall) and spontaneously hypertensive rats (SHR). In the sanie honiogenate, ANG Ⅱ was degraded to ANG Ⅲ, and ANG Ⅲ remained unchanged during 15 min incubation. However, all the homapenates were able to catalyse hippuryl-L-histiayl'-L-leucine to hippuric acid and the catalysis was completely inhibited by 3 PM captorpil. The data showed that angiotensin converting enzyrne presenl in the hypothalamus when extracted by the normal laboratory procedures was not able to hydrolyse ANG Ⅰ to ANG Ⅱ. In addition, the aminopeptidase that degraded ANG Ⅰ to des-Asp-ANG Ⅰ was not inhibited by amastatin, bestatin and EDTA, indicating that it is not aminopeptidase A or B. The formation of hippuric acid was significantly higher in the homapenate of the LRAS whilst the SHR and DOCA-salt showed a significant higher rate of des-Asp-ANG Ⅰ formation. These findings are the first dernonsiration of the formation of des-Asp-ANG Ⅰ as the major product of ANG Ⅰ degradation in the hypothalamic homagenate of the rat. The data also show that in two models of low renin hypertensive rats the formation of nanopeptide is significantly elevated.
文摘Objective To detect the A/C1165 polymorphism of angiotensin Ⅱ type Ⅰ receptor (AT1-R)gene in essential hypertensive elderly. Methods The A/C1166 polymorphism of AT1-R gene was assessed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) in a case-control study of 87 essential hypertensive elders (EH) and 55 normolensive elders (NT). Results The genotype frequencies of AA, AC, CC were 0 .805 , 0.161, 0 .034 in EH group and 0 .927 ,0 .073 ,0 .000 in NT group respectively. The frequency of C61166 allele was higher in EH group (0.115) than in NT group (0 .036 )(P<0 .05 ). Conclusion The resultsindicate that A/C1166 polymorphism of AT1-R gene may be associated with essential hypertension in elderly.
