The Jayachamarajapura schist belt in western Dharwar craton, southern India, is predominantly an ultramafics dominant terrain. These rocks have been extensively metamorphosed and altered to serpentinite. The komatiite...The Jayachamarajapura schist belt in western Dharwar craton, southern India, is predominantly an ultramafics dominant terrain. These rocks have been extensively metamorphosed and altered to serpentinite. The komatiite nature of ultramafics is conspicuous. In most of the areas of the belt these ultramafics are massive in nature. However, some of the ultramafic units show layered nature. But, their outcrops are encompassed within the massive komatiitic bodies. These komatiitic ultramafics are predominantly Mg-rich in nature. The layered rocks are also Mg-rich, and their field setting and geochemistry suggest their intermittent occurrence as sills, during the differentiation of peridotitic magma. The layered rocks, which have been intensely serpentinisation show homogenous nature. They are almost wholly made of serpentine with occasional relics of pyroxene. Secondary carbonate mineral is often noticed. Their higher MgO content indicates Mg-rich ultramafic magmatism during Archaean orogeny.展开更多
Chronic pancreatitis (CP) is a progressive inflammatory disorder of the pancreas. It is predominantly idiopathic (with an unknown cause) in India and mostly due to alcohol in the West. Diabetes that occur secondary to...Chronic pancreatitis (CP) is a progressive inflammatory disorder of the pancreas. It is predominantly idiopathic (with an unknown cause) in India and mostly due to alcohol in the West. Diabetes that occur secondary to chronic pancreatitis (T3c Diabetes) is often brittle, and is difficult to attain normoglycemia with conventional treatment requiring multiple doses of insulin. Mild and severe model of CP was induced in mice by repeated intraperitoneal injections of cerulein and L-arginine respectively with an intent to study islet dysfunction and develop therapeutic strategy in animal models of CP. Dietary intervention of epigallocatechin-3-gallate (EGCG) was tested in both the models of CP for its beneficial effects on insulin secretory functions. Pancreata collected upon euthanasia were used to study alterations in the morphology of pancreatic parenchyma and inflammation by staining with H&E and fibrotic changes by Masson’s trichrome and picrosirius staining. Insulin secretory functions of islets were evaluated to test the efficacy of the dietary intervention on β-cell functions. Intraperitoneal glucose tolerance test was performed to monitor the glucose homeostasis before and after the dietary intervention. Both the models resulted in CP with dispersed acini, inflammation and fibrosis. The loss of acini and extent of fibrosis was more in L-arginine model. 2-fold improvement in glucose-stimulated insulin secretory functions of islets was observed with 0.5% EGCG dietary intervention in cerulein model of CP and 1.6-fold in L-arginine model of CP. A further improvement in insulin secretion by 3.2-fold was observed with additional dietary supplements like N-acetyl cysteine, curcumin in combination with EGCG. Our results thus demonstrate and highlight the therapeutic potential of dietary green tea (EGCG) supplementation in reversing islet dysfunction and improving glucose homeostasis in experimental chronic pancreatitis in mice.展开更多
文摘The Jayachamarajapura schist belt in western Dharwar craton, southern India, is predominantly an ultramafics dominant terrain. These rocks have been extensively metamorphosed and altered to serpentinite. The komatiite nature of ultramafics is conspicuous. In most of the areas of the belt these ultramafics are massive in nature. However, some of the ultramafic units show layered nature. But, their outcrops are encompassed within the massive komatiitic bodies. These komatiitic ultramafics are predominantly Mg-rich in nature. The layered rocks are also Mg-rich, and their field setting and geochemistry suggest their intermittent occurrence as sills, during the differentiation of peridotitic magma. The layered rocks, which have been intensely serpentinisation show homogenous nature. They are almost wholly made of serpentine with occasional relics of pyroxene. Secondary carbonate mineral is often noticed. Their higher MgO content indicates Mg-rich ultramafic magmatism during Archaean orogeny.
文摘Chronic pancreatitis (CP) is a progressive inflammatory disorder of the pancreas. It is predominantly idiopathic (with an unknown cause) in India and mostly due to alcohol in the West. Diabetes that occur secondary to chronic pancreatitis (T3c Diabetes) is often brittle, and is difficult to attain normoglycemia with conventional treatment requiring multiple doses of insulin. Mild and severe model of CP was induced in mice by repeated intraperitoneal injections of cerulein and L-arginine respectively with an intent to study islet dysfunction and develop therapeutic strategy in animal models of CP. Dietary intervention of epigallocatechin-3-gallate (EGCG) was tested in both the models of CP for its beneficial effects on insulin secretory functions. Pancreata collected upon euthanasia were used to study alterations in the morphology of pancreatic parenchyma and inflammation by staining with H&E and fibrotic changes by Masson’s trichrome and picrosirius staining. Insulin secretory functions of islets were evaluated to test the efficacy of the dietary intervention on β-cell functions. Intraperitoneal glucose tolerance test was performed to monitor the glucose homeostasis before and after the dietary intervention. Both the models resulted in CP with dispersed acini, inflammation and fibrosis. The loss of acini and extent of fibrosis was more in L-arginine model. 2-fold improvement in glucose-stimulated insulin secretory functions of islets was observed with 0.5% EGCG dietary intervention in cerulein model of CP and 1.6-fold in L-arginine model of CP. A further improvement in insulin secretion by 3.2-fold was observed with additional dietary supplements like N-acetyl cysteine, curcumin in combination with EGCG. Our results thus demonstrate and highlight the therapeutic potential of dietary green tea (EGCG) supplementation in reversing islet dysfunction and improving glucose homeostasis in experimental chronic pancreatitis in mice.
