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Genetic Diversity Within a Jackfruit (Artocarpus heterophyllus Lam.) Germplasm Collection in China Using AFLP Markers 被引量:7
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作者 LI Ying-zhi MAO Qi FENG Feng YE Chun-hai 《Agricultural Sciences in China》 CAS CSCD 2010年第9期1263-1270,共8页
Jackfruit is cross-pollinated and mostly seed propagated, a wide range of variation exists in fruit quality. With the development of efficient vegetative propagation methods, excellent genotypes selected from these se... Jackfruit is cross-pollinated and mostly seed propagated, a wide range of variation exists in fruit quality. With the development of efficient vegetative propagation methods, excellent genotypes selected from these seed propagated seedlings will gradually replace other genotypes in jackfruit producing areas. In this study, genetic diversity of 50 jackfruit accessions from three provinces in China was analyzed based on amplified fragment length polymorphic (AFLP) markers. A total of 320 unambiguous bands were produced by eight primer combinations, and 65 (20.3%) of them were polymorphic. Genetic similarity coefficients ranged from 0 to 0.9841, with an average of 0.5000, indicating a moderate genetic diversity in this collection. The dendrogram derived by unweighted pair group method with arithmetic mean algorithm (UPGMA) analysis revealed five groups, and no correlation between genetic relationship and geographical origin were found. Accessions of soft and firm flesh type were not clustered into distinct groups, neither could yearly bearing once, or twice fruit accessions. This study has provided useful information for collection and preservation of jackfruit germplasm worldwide. 展开更多
关键词 jackfruit genetic diversity AFLP molecular markers
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From Jackfruit Rags to Hierarchical Porous N-Doped Carbon: A High-Performance Anode Material for Sodium-Ion Batteries 被引量:5
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作者 Baisheng Zhao Yichun Ding Zhenhai Wen 《Transactions of Tianjin University》 EI CAS 2019年第5期429-436,共8页
Renewable biomass-derived carbon materials have attracted increasing research attention as promising electrode materials for electrochemical energy storage devices, such as sodium-ion batteries (SIBs), due to their ou... Renewable biomass-derived carbon materials have attracted increasing research attention as promising electrode materials for electrochemical energy storage devices, such as sodium-ion batteries (SIBs), due to their outstanding electrical conductivity, hierarchical porous structure, intrinsic heteroatom doping, and environmental friendliness. Here, we investigate the potential of hierarchical N-doped porous carbon (NPC) derived from jackfruit rags through a facile pyrolysis as an anode material for SIBs. The cycling performance of NPC at 1 A/g for 2000 cycles featured a stable reversible capacity of 122.3 mA h/g with an outstanding capacity retention of 99.1%. These excellent electrochemical properties can be attributed to the unique structure of NPC;it features hierarchical porosity with abundant carbon edge defects and large speci c surface areas. These results illuminate the potential application of jackfruit rags-derived porous carbon in SIBs. 展开更多
关键词 POROUS CARBON N-DOPED CARBON Sodium-ion battery Anode jackfruit rags Energy storage and conversion
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Diet Chocolates and Replacement of Cocoa Powder with Jackfruit Seed Powder 被引量:1
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作者 Akshaya Ravindran Maya Raman +3 位作者 Ninisha Babu Ammu Dinakaran T. V. Sankar T. K. Srinivasa Gopal 《Food and Nutrition Sciences》 2020年第3期220-233,共14页
Chocolate is a very popular food product that is relished by millions of people for its unique, rich and sweet taste. The health benefits of chocolate are immense which is attributed to its high polyphenolic content. ... Chocolate is a very popular food product that is relished by millions of people for its unique, rich and sweet taste. The health benefits of chocolate are immense which is attributed to its high polyphenolic content. In the current study, the diet chocolates using natural and artificial sweeteners were developed. Also, the jackfruit seed powder after fermentation (anaerobic and aerobic) was used to replace cocoa powder by 10%. The physiochemical and glycemic index of the chocolates were analyzed. The physiochemical analysis of the chocolates indicated that substitution of jackfruit seed powder did not alter significantly the proximate composition. It was found out that the glycemic index and glycemic load of the chocolates were significantly lower than commercial milk chocolate, which makes it a suitable food product for diabetic and other groups. The samples were packed in pouches made of metallised polyester films laminated with polyethylene for storage studies. Instrumental textural studies were in concordance with sensory observation, indicating that the product remained stable for 3 months at room temperature (26&deg;C ± 2&deg;C). The color of the product remained similar throughout the storage period indicating stable structural and surface characteristics. Hence, the chocolates developed using natural and artificial sweeteners have low GI and will minimize the risk of several chronic diseases including diabetes and the use of jackfruit seed powder as a substitute for cocoa powder has not altered any of the characteristic features of chocolate. 展开更多
关键词 Diet CHOCOLATE STEVIA SUCRALOSE jackfruit SEED POWDER Glycemic Index
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Standardization of Leaf Sampling Technique in Jackfruit Nutrient Status Diagnosis 被引量:1
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作者 Yan Sun Jianfeng Yang +3 位作者 Hua Wang Chao Zu Lehe Tan Gang Wu 《Agricultural Sciences》 2015年第2期232-237,共6页
In China, little information is known about the nutrient requirements of jackfruits and the traditional nutrient management usually depends on the experience. Therefore, in this study, an attempt was made to standardi... In China, little information is known about the nutrient requirements of jackfruits and the traditional nutrient management usually depends on the experience. Therefore, in this study, an attempt was made to standardize the leaf sampling technique and the suitable range of leaf nutrient concentrations for jackfruit (Artocarpus heterophyllus Lam.) nutrient status diagnosis. The sampling result was affected by canopy height, leaf age and time of sampling. Therefore, the three factors were studied. The results illustrated that the stability in level of nutrient concentrations was in 3 - 6 month-old leaves from the central part of the canopy. The most stable period was from April to May for leaf sampling. It was recommended that the stable intracanopy and stable period of nutrient concentrations could be used as the standards of leaf sampling technique. Based on the leaf sampling technique, the standard of leaf nutrient concentrations was summarized, and could be used as the standard of nutrient suitability evaluation. 展开更多
关键词 jackfruit NUTRIENT STATUS DIAGNOSIS LEAF Sampling Technique STANDARDIZATION
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The Phytochemical and Antioxidant Characteristics of Fermented Jackfruit (Artocarpus heterophyllus L.) Leaves Using Single and Mixed Starter Culture
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作者 Norhazniza Aziz Koh Soo Peng +2 位作者 Rosmawati Abdullah NurSyazwani Abdul Hamid Razali Mustaffa 《Journal of Food Science and Engineering》 2018年第1期55-60,共6页
The phytochemical and antioxidant properties of fermented jackfruit leaves, using yeast and acetic acid bacteria, individually or in combination during 6 days of fermentation were investigated in the present study. Ch... The phytochemical and antioxidant properties of fermented jackfruit leaves, using yeast and acetic acid bacteria, individually or in combination during 6 days of fermentation were investigated in the present study. Changes in pH, total reducing sugar, ethanol, acetic acid, total phenolics and flavanoids content were examined. A number of antioxidant activities such as ferric reducing antioxidant power (FRAP) and DPPH free radical scavenging activities were comparatively tested to determine the differences in the respective properties during the fermentation process. Results obtained demonstrated that there were differences in the phytochemical and antioxidant properties of fermented jackfruit leaves, depending on the fermentation starters. Fermented leaves using acetic acid bacteria alone, exhibited higher total reducing sugar (6.89 mg glucose/mL) compared to others. The ferric-reducing activities of fermented jackfruit leaves showed an increasing trend in the yeast fermentation process (5.96-10.12 mg ascorbic acid equivalent/mL). Generally, all extracts of fermented jackfruit leaves exhibited stronger DPPH radical scavenging activity except in the total phenolics and flavonoids content, whereby it showed a decrease trend throughout the fermentation process. 展开更多
关键词 jackfruit leaves acetic acid bacteria YEAST fermentation antioxidant.
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Comparative Study of Biogas Production from Jackfruit Waste, Banana Peels, and Pineapple Peels Co-Digested with Cow Dung
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作者 Tadeo Mibulo Denis Nsubuga +2 位作者 Isa Kabenge Nicholas Kiggundu Kerstin D. Wydra 《Journal of Sustainable Bioenergy Systems》 CAS 2023年第1期1-15,共15页
Only 42% of Uganda’s population has access to electricity. The population continues to use firewood and charcoal as a source of energy, which leads to depletion of forests thus to climate change. The purpose of this ... Only 42% of Uganda’s population has access to electricity. The population continues to use firewood and charcoal as a source of energy, which leads to depletion of forests thus to climate change. The purpose of this study was to assess the potential of biogas production from jackfruit waste, banana peels, and pineapple peels when co-digested with cow dung as an alternative energy source. Substrates for each waste were co-digested with varying proportions (0%, 25%, and 50%) of cow dung using laboratory-scale 250 mL anaerobic digestors. The total biogas generation for jackfruit waste, banana peels, and pineapple peels after 30 days of anaerobic digestion was 82.3, 189, and 262 mL, respectively. When jack fruit waste, pineapple peels and banana peels were co-digested with 25% cow dung, the total amount of biogas produced increased by a factor of two and three, respectively. However, 50% of cow dung only significantly (p ≤ 0.05) improved for jack fruit waste by two folds. Therefore, the results indicated that jackfruit waste, banana and pineapple peels can be used for biogas production to augment energy supply. . 展开更多
关键词 BIOGAS CO-DIGESTION jackfruit Waste BANANA Pineapple Peels
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Recent application of jackfruit waste in food and material engineering:A review 被引量:3
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作者 S.B.Kalse S.B.Swami 《Food Bioscience》 SCIE 2022年第4期252-262,共11页
Jackfruit(Artocarpus heterophyllus Lam.)processing industries generate considerable amount of solid wastes in the form of peels,seeds and latex which leads environmental pollution these waste can be utilized as an att... Jackfruit(Artocarpus heterophyllus Lam.)processing industries generate considerable amount of solid wastes in the form of peels,seeds and latex which leads environmental pollution these waste can be utilized as an attractive source for different sustainable products.Peel has a potential and alternative source of commercial pectin,activated carbon preparation,adsorbent to remove the various harmful dyes and metal ions from aqueous water and also utilized as a sustainable fuel as a bio hydrogen,biogas and ethanol production.Jackfruit seed flour having potentiality to replace wheat flour(25-30%)in bakery industry and it can also use as substrate for the production of red pigments which can be used as colorant in textile industry also useful for the preparation of Fast Dissolving Tablets.Jackfruit latex utilized as the cementing medium,irrigation solution,denture cleaning solution,resin and other future dental filling material used as cost effective material.By-product recovery from jackfruit wastes not only can improve the overall economics of processing units but also reduce the problem of environmental pollution considerably.This review demonstrates the potential utilization of jackfruit waste. 展开更多
关键词 jackfruit Waste utilization BIO-ENERGY Fast dissolve tablet PECTIN
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Optimization of the iron-enriched extruded snack containing jackfruit seed flour, mung bean flour and ferrous ammonium phosphate by using response surface methodology
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作者 Aparna Kumari Aditya Gupta Anil Kumar Chauhan 《Food Production, Processing and Nutrition》 2022年第1期397-407,共11页
The Food and Agriculture Organization (State of food and agriculture. Moving forward on food loss and waste reduction, 2019) stated that approximately 1.3 billion tons of food wasted every year caused the emission of ... The Food and Agriculture Organization (State of food and agriculture. Moving forward on food loss and waste reduction, 2019) stated that approximately 1.3 billion tons of food wasted every year caused the emission of around 4.4 gigatons of greenhouse gas. World Health Organization highlights that iron deficiency affects billions of people worldwide and remains the leading cause of anemia. With the growth of healthy and sustainable diets and consumers’ better understanding of the relationship between diet, health, and the environment, there is an opportunity to develop novel healthy extruded snacks. The present study was carried out using response surface methodology to investigate the effects of varying proportions of jackfruit seed flour, mung bean flour, and ferrous ammonium phosphate on physical and functional properties of extruded snacks. Experiments were carried out using a central composite rotatable design with three independent parameters such as jackfruit seed flour(%), mung bean flour(%), and ferrous ammonium phosphate (%), and five responses such as bulk density (BD), expansion ratio (ER), hardness, color difference (ΔE), and iron content. It was found that ferrous ammonium phosphate significantly increased the iron content. Bulk density, expansion ratio, and hardness were significantly affected by jackfruit seed flour and mung bean flour. All independent variables significantly influenced colour value. The optimized iron-enrich extruded snacks were obtained at 24.87% jackfruit seed flour, 20.95% mung bean flour, and 0.021% ferrous ammonium phosphate. The optimized extruded snack contained 15.32 g/100 g protein and 22.03 mg/100 g iron content. Results of the study indicate that the optimized extruded snack could be used as a protein and iron source to mitigate malnutrition and anemia. 展开更多
关键词 jackfruit seed flour Mung bean flour Ferrous ammonium phosphate Extruded snack Anemia
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牡丹籽种皮菠萝蜜丝酵素的研制
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作者 陈凤真 付高杰 +1 位作者 张振中 赵宝义 《菏泽学院学报》 2024年第5期117-122,共6页
本实验以菠萝蜜丝和牡丹籽种皮为原料,以柠檬酸、白砂糖、果蔬发酵剂为辅料来研制酵素.首先采用单因素实验确定菠萝蜜丝、白砂糖和柠檬酸最佳添加量,然后通过正交实验对牡丹籽种皮菠萝蜜丝酵素的最佳配方进行优化,并对最佳配方研制的酵... 本实验以菠萝蜜丝和牡丹籽种皮为原料,以柠檬酸、白砂糖、果蔬发酵剂为辅料来研制酵素.首先采用单因素实验确定菠萝蜜丝、白砂糖和柠檬酸最佳添加量,然后通过正交实验对牡丹籽种皮菠萝蜜丝酵素的最佳配方进行优化,并对最佳配方研制的酵素进行微生物测定.结果表明:牡丹籽种皮菠萝蜜丝酵素的最佳配方为菠萝蜜丝的添加量为30%、柠檬酸添加量为0.3%、白砂糖添加量为20%;研制的酵素颜色呈淡黄色,具有淡淡的清香,口感香甜细腻,味道酸甜可口,符合国家安全标准.研制的牡丹籽种皮菠萝蜜丝对酵素的风味具有创新作用,同时提高了牡丹种皮与菠萝蜜丝的附加值,降低了酵素的研制成本. 展开更多
关键词 菠萝蜜丝 牡丹籽种皮 酵素
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超声辅助酶法提取菠萝蜜果皮果胶工艺优化及低酯化改性 被引量:2
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作者 韦鑫 黄安 +2 位作者 陈逸 秦靖欣 杨志伟 《食品工业科技》 CAS 北大核心 2024年第16期239-248,共10页
为提高菠萝蜜果皮果胶(Jackfruit peel pectin,JFPP)的得率,并得到高纯度具有良好的功能特性的果胶,本研究通过响应面试验优化超声辅助纤维素酶法对果胶的提取工艺,对提取的果胶进行初步纯化,包括脱脂、脱蛋白、脱色和透析浓缩,并对纯... 为提高菠萝蜜果皮果胶(Jackfruit peel pectin,JFPP)的得率,并得到高纯度具有良好的功能特性的果胶,本研究通过响应面试验优化超声辅助纤维素酶法对果胶的提取工艺,对提取的果胶进行初步纯化,包括脱脂、脱蛋白、脱色和透析浓缩,并对纯化后果胶进行酶法低酯化(De-esterification)改性,得到低酯菠萝蜜果皮果胶(DE-JFPP),对比分析改性前后果胶的基本成分组成、分子量、单糖组成、官能团特征、热稳定性和微观结构。结果表明,最优提取工艺参数为加酶量0.7%,酶解温度30.4℃,pH4.6,此条件下JFPP得率为15.26%±0.07%。改性后的果胶的半乳糖醛酸含量由79.10%增加到83.24%,总糖含量由43.42%降低为41.90%,酯化度由84.25%降低为32.36%,重均分子量由29.24 ku降低为28.49 ku。JFPP和DE-JFPP均有11种单糖,均属于RGI型果胶,DE-JFPP支链化程度减小。傅里叶变换红外光谱显示改性前后的果胶均具有果胶特征吸收峰,改性后的果胶分子对应酯化羧基的吸收峰峰面积下降。JFPP和DE-JFPP的溶解温度分别为160.22℃和123.28℃,降解温度分别为255.11℃和230.56℃,均具有较高的热稳定性。扫描电镜图显示,JFPP表面光滑,结构紧凑,DEJFPP表面粗糙,结构疏松。本研究优化了JFPP提取工艺,提高其得率,并证明低酯化后的果胶具有较好的理化性质及微观结构,可满足不同领域中对凝胶负载体系需求,进一步拓展菠萝蜜果皮果胶的利用空间。 展开更多
关键词 菠萝蜜果皮 果胶 超声辅助酶法 响应面优化 低酯化 理化性质
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微孔气调包装对鲜切菠萝蜜保鲜效果的影响 被引量:1
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作者 赵文汇 刘欣瑶 +4 位作者 梁金甜 樊爱萍 潘艳南 唐莉 曾丽萍 《包装工程》 CAS 北大核心 2024年第5期65-73,共9页
目的为探究微孔气调包装(MMAP)对鲜切菠萝蜜的保鲜效果,明确O_(2)体积分数、CO_(2)体积分数和打孔数的最佳处理条件。方法本研究以菠萝蜜为原料,通过单因素和正交-模糊综合评价法,研究冷藏期间MMAP对鲜切菠萝蜜品质(菌落总数、质量损失... 目的为探究微孔气调包装(MMAP)对鲜切菠萝蜜的保鲜效果,明确O_(2)体积分数、CO_(2)体积分数和打孔数的最佳处理条件。方法本研究以菠萝蜜为原料,通过单因素和正交-模糊综合评价法,研究冷藏期间MMAP对鲜切菠萝蜜品质(菌落总数、质量损失率、可溶性固形物(TSS)、色差、可滴定酸(TA)、硬度、VC含量和丙二醛(MDA)质量摩尔浓度)的影响。结果与对照相比,MMAP抑制了微生物生长,维持了较高TSS、TA和VC含量,减少了MDA的积累,提高了鲜切菠萝蜜的整体品质,延长货架期至少6 d。筛选出MMAP最佳处理条件:O_(2)体积分数为5%,CO_(2)体积分数为6%,216 cm^(2)的打孔数为15个。结论MMAP是一种安全、低廉、简单的保鲜技术,可有效抑制鲜切菠萝蜜在贮藏过程中组织软化、营养损失、风味变化等品质裂变问题,具有很大的工业应用潜力。 展开更多
关键词 鲜切菠萝蜜 微孔气调包装 优化 保鲜
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干旱胁迫诱导菠萝蜜裂皮病发生的生理基础研究 被引量:1
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作者 李少卡 张世青 +3 位作者 颜彩缤 赵亚 肖敏 范鸿雁 《中国热带农业》 2024年第2期35-43,共9页
菠萝蜜是我国重要的热带水果之一。为研究菠萝蜜裂皮病关键致病因子及干旱诱导菠萝蜜裂皮病发生机制,通过设计不同盆栽控水试验,分别测定40%、30%、20%和10%不同梯度水分和干旱3d、6d、9d胁迫菠萝蜜幼苗后的生长性状和光合作用指标。试... 菠萝蜜是我国重要的热带水果之一。为研究菠萝蜜裂皮病关键致病因子及干旱诱导菠萝蜜裂皮病发生机制,通过设计不同盆栽控水试验,分别测定40%、30%、20%和10%不同梯度水分和干旱3d、6d、9d胁迫菠萝蜜幼苗后的生长性状和光合作用指标。试验结果表明,10%水分干旱处理下,对‘琼引1号’和‘琼引8号’菠萝蜜叶面积、叶长和叶宽和叶绿素含量等指标影响最大,叶片增长量均小于0;叶绿素含量及净光合速率等光合指标显著下降,均极显著低于CK。干旱9d胁迫下,两个品种的叶面积变化较大,其增长率分别为-33.48%和-42.16%,其次为叶长的增长率-27.82%和-34.12%,均小于0,显著小于CK的增长率;叶绿素含量及净光合速率等光合指标显著下降,均极显著低于CK;不同干旱短时间胁迫下对茎围和株高影响不显著。在10%~40%水分含量范围内,随着土壤水分含量降低和干旱时间增加,菠萝蜜植物生长性状受影响越明显,其影响程度依次为水分含量10%>20%>30%>40%、干旱时间9d>6d>3d的趋势。研究阐明不同干旱胁迫对菠萝蜜生长及光合生理系统的影响,为干旱诱导下菠萝蜜裂皮病致病机制研究和生产中合理水分利用提供基础支撑。 展开更多
关键词 菠萝蜜 干旱 生长性状 光合指标
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土壤质地对菠萝蜜根际微生物碳源利用、线虫群落及果实糖分的影响
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作者 苏兰茜 濮秋婕 +4 位作者 白亭玉 吴月仙 吴刚 谭乐和 胡雅莉 《中国农业科学》 CAS CSCD 北大核心 2024年第2期349-362,共14页
【目的】研究相同施肥模式下不同质地土壤微环境差异及其与果实关键品质(糖分)的潜在关系,为区域性的果园培肥和增产提质提供理论依据。【方法】选取施肥方案一致、土壤质地分别为壤土、砂壤土和砂土的‘马来西亚1号’标准菠萝蜜园,采... 【目的】研究相同施肥模式下不同质地土壤微环境差异及其与果实关键品质(糖分)的潜在关系,为区域性的果园培肥和增产提质提供理论依据。【方法】选取施肥方案一致、土壤质地分别为壤土、砂壤土和砂土的‘马来西亚1号’标准菠萝蜜园,采集养树期、花期和果期的菠萝蜜根周土壤和成熟果实,研究土壤微生物群落代谢功能、线虫群落结构、土壤理化性质及果实糖分的变化及潜在关系。【结果】砂壤土和砂土微生物碳源利用变化趋势均为养树期>花期>果期。壤土微生物碳源代谢强度大小为果期>养树期>花期。土壤微生物主要的碳源利用类型是碳水化合物类、氨基酸类、羧酸类和多聚物类碳源。砂土各时期的土壤微生物群落结构多样性指数和各营养类群线虫丰度普遍高于其他两种土壤,且在养树期和果期较高。壤土各时期线虫生态指数和土壤pH、有机质普遍高于其他两种土壤。果实中的葡萄糖含量表现为砂壤土>壤土>砂土,其他糖组分在各质地土壤中差异不显著。采样时期对土壤线虫群落多样性有显著的直接效应,土壤质地对菠萝蜜果实糖分有显著的间接效应和总效应。【结论】有机、无机肥配施对异质土壤的微生态环境影响各异,土壤质地通过直接或间接影响养树期和果期菠萝蜜根际微生物和线虫群落结构及功能,最终影响土壤养分转化和果实品质。 展开更多
关键词 土壤质地 菠萝蜜 微生物代谢多样性 线虫群落 果实糖分
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菠萝蜜果皮废弃生物质的多糖提取工艺及其絮凝效果研究
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作者 曾凤娟 高世伟 +2 位作者 黄姗 冯莉莉 杨耀红 《环境科学与管理》 CAS 2024年第11期100-105,共6页
文章探究菠萝蜜多糖的最佳提取工艺,通过单因素分析法和正交实验法分析料液比,提取时间、提取温度、pH等对菠萝蜜多糖提取量的影响,研究菠萝蜜多糖及其与聚合硫酸铁复合复配使用时水体浊度去除效果。实验结果表明:菠萝蜜多糖提取最优工... 文章探究菠萝蜜多糖的最佳提取工艺,通过单因素分析法和正交实验法分析料液比,提取时间、提取温度、pH等对菠萝蜜多糖提取量的影响,研究菠萝蜜多糖及其与聚合硫酸铁复合复配使用时水体浊度去除效果。实验结果表明:菠萝蜜多糖提取最优工艺条件为:固液比为1:30,提取温度为80℃,提取时间为60 min,菠萝蜜果皮多糖平均含量可达到22.35±0.2 mg/mL;菠萝蜜多糖与聚合硫酸铁复配使用能显著去除水体浊度,复配絮凝剂PFS+JF的投放量为(13.79+75.32)mg/L,浊度去除率即可达到98%。结果表明,该菠萝蜜多糖提取工艺安全环保,助凝效果明显,具有良好的应用前景。 展开更多
关键词 菠萝蜜 多糖 提取工艺 助凝剂 浊度去除
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菠萝和菠萝蜜复合果醋发酵工艺及挥发性物质分析
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作者 颜韶波 黄和 陈菊 《食品研究与开发》 CAS 2024年第11期143-151,共9页
为进一步丰富复合果醋的种类,提升果醋的香气,以菠萝和菠萝蜜为原料进行多菌种混合发酵,以果醋总酸和感官评分为指标,采用单因素和正交试验优化发酵工艺条件,以顶空固相微萃取(headspace solid-phase microextraction,HS-SPME)结合气相... 为进一步丰富复合果醋的种类,提升果醋的香气,以菠萝和菠萝蜜为原料进行多菌种混合发酵,以果醋总酸和感官评分为指标,采用单因素和正交试验优化发酵工艺条件,以顶空固相微萃取(headspace solid-phase microextraction,HS-SPME)结合气相色谱-质谱联用(gas chromatography-mass spectrometry,GC-MS)技术分析菠萝和菠萝蜜复合果醋的挥发性物质。结果表明,最优工艺条件为菠萝与菠萝蜜体积比6∶4,醋酸发酵阶段,初始pH4.5、乙醇体积分数6%、醋酸菌接种量11%、发酵温度28℃,在该条件下发酵8 d,复合果醋的总酸(以乙酸计)为31.92 g/L。进一步检测其挥发性物质,共分析出乙酸、3-羟基丁酸乙酯、乙酸异戊酯、异戊醇、苯乙醇和苯甲醛等54种主要挥发性物质。该文对菠萝和菠萝蜜复合果醋发酵工艺及挥发性物质进行研究,为复合果醋的开发提供参考。 展开更多
关键词 菠萝 菠萝蜜 复合果醋 发酵工艺优化 顶空固相微萃取 气相色谱-质谱联用
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间作模式对幼龄菠萝蜜果园土壤养分和微生物群落的影响
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作者 颜彩缤 赵亚 +2 位作者 肖敏 李少卡 范鸿雁 《中国果树》 2024年第1期78-84,共7页
为探明不同间作模式对幼龄菠萝蜜园土壤养分和土壤微生物的影响规律,选取菠萝蜜单作和菠萝蜜间作南瓜、平托花生、硬皮豆4种种植模式下的根际土壤样本,测定土壤养分以及对根际土壤微生物群落进行16S rRNA V4-V5区检测,并与试验区间作前... 为探明不同间作模式对幼龄菠萝蜜园土壤养分和土壤微生物的影响规律,选取菠萝蜜单作和菠萝蜜间作南瓜、平托花生、硬皮豆4种种植模式下的根际土壤样本,测定土壤养分以及对根际土壤微生物群落进行16S rRNA V4-V5区检测,并与试验区间作前的基础土壤进行对比分析。结果表明:3种间作模式较间作前均显著提高了土壤有机碳、全氮、速效氮、全磷、全钾含量,较单作模式均显著提高了土壤全氮、速效氮和全钾含量,土壤pH值均高于单作模式,间作硬皮豆效果最佳;4种种植模式的细菌优势门类群分别是酸杆菌门、变形菌门、放线菌门和绿弯菌门等,间作均提高了根瘤菌属相对丰度,降低了酸杆菌门、放线菌门、绿弯菌门相对丰度;酸杆菌门与速效磷含量呈显著正相关,放线菌门与速效氮、全钾、全氮含量呈极显著或显著负相关;菠萝蜜株高增长情况表现为菠萝蜜间作硬皮豆>菠萝蜜间作南瓜>菠萝蜜间作平托花生>菠萝蜜单作。 展开更多
关键词 菠萝蜜 间作模式 土壤养分 土壤微生物群落
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基于UPLC-Q/TOF-MS技术分析发酵胶原蛋白肽-菠萝蜜汁的代谢物变化
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作者 李云龙 马婷婷 +4 位作者 朱科学 裴国民 朱露露 曹君 李川 《食品研究与开发》 CAS 2024年第11期39-46,共8页
该文采用超高效液相色谱-四极杆飞行时间质谱(ultra-high performance liquid chromatography quadrupole timeof-flight mass spectrometry,UPLC-Q/TOF-MS)技术采集胶原蛋白肽溶液(collagen peptide solution,PP)、未发酵菠萝蜜汁(non-... 该文采用超高效液相色谱-四极杆飞行时间质谱(ultra-high performance liquid chromatography quadrupole timeof-flight mass spectrometry,UPLC-Q/TOF-MS)技术采集胶原蛋白肽溶液(collagen peptide solution,PP)、未发酵菠萝蜜汁(non-fermented jackfruit juice,JJ)、发酵菠萝蜜汁(fermented jackfruit juice,FJJ)和混合发酵胶原蛋白肽-菠萝蜜汁(co-fermented peptide-jackfruit juice,FPJ)的全代谢物组分,比较FPJ组与FJJ、JJ、PP组的代谢物差异。在正、负离子模式下分别鉴定出1 388个和565个代谢物特征。采用VIP>1.0和Fold Change>2作为阈值,使用代谢组学数据库对差异代谢物精准确定,筛选出80种代谢物作为关键代谢物,包含42种寡肽、7种醇、6种氨基酸、3种有机酸、3种酯类、3种脂质、2种醛等。结果表明,FPJ组较FJJ组和JJ组中氨基酸、小肽、酯类、酸类和风味物质的含量发生明显变化,FPJ组的营养价值和感官品质得到改善。 展开更多
关键词 发酵胶原蛋白肽-菠萝蜜汁 超高效液相色谱-四极杆飞行时间质谱 代谢组学 多元统计分析 关键代谢物
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波罗蜜柠檬酸合酶基因(AheCS)的表达及其与柠檬酸积累的相关性分析
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作者 李思彤 李真琴 +1 位作者 林婉彤 王俊宁 《福建农业学报》 CAS CSCD 北大核心 2024年第2期154-164,共11页
【目的】分析波罗蜜柠檬酸合酶基因(Artocarpus heterophyllus citrate synthase,AheCS)的生物学功能,研究柠檬酸含量与AheCS基因相对表达量的相关性,为探究AheCS基因在波罗蜜(Artocarpus heterophyllus Lam.,Ahe)果实有机酸代谢中的可... 【目的】分析波罗蜜柠檬酸合酶基因(Artocarpus heterophyllus citrate synthase,AheCS)的生物学功能,研究柠檬酸含量与AheCS基因相对表达量的相关性,为探究AheCS基因在波罗蜜(Artocarpus heterophyllus Lam.,Ahe)果实有机酸代谢中的可能作用提供参考。【方法】以海大2号波罗蜜果实为材料,采用0.5 mg·L^(-1)1-甲基环丙烯(1-Methylcyclopropene,1-MCP)和1000 mg·L^(-1)外源乙烯利(ethrel,ETH)(40%)处理,研究室温(22±1)℃、相对湿度90%条件下波罗蜜果实成熟过程中柠檬酸的动态变化,克隆获得3个波罗蜜的CS基因(AheCS1、AheCS2和AheCS3),对其进行生物信息学分析,同时分析3个AheCS基因在不同采后处理下(自然成熟、外源乙烯催熟和1-MCP延缓成熟)的相对表达情况及其与柠檬酸含量的相关性。【结果】随着波罗蜜果实的自然成熟,果实中柠檬酸含量呈先上升后下降的变化趋势;外源ETH处理加速了柠檬酸的合成速率,提前了峰值出现的时间;1-MCP处理抑制了贮藏前期柠檬酸含量下降速率,推迟了高峰出现时间。生物信息学分析发现,AheCS1/2/3基因的开放阅读框(ORF)长1422~1827 bp,编码蛋白都含有柠檬酸合酶保守结构域WPNVDAHS,属于柠檬酸合酶家族成员。AheCS1/2/3蛋白氨基酸序列分别与柑橘(Citrus reticulata Blanco.)CsCS(MH_048698.1)、川桑(Morus notabilis C.K.Schneid.)MnCs(XP010087965.1)、红掌(Anthurium andraeanum Linden.)AaCS(JAT55223.1)亲缘关系最近,相似性分别达到86.49%、97.00%、86.00%。qRT-PCR结果显示:AheCS1/2/3基因在果实自然成熟(CK)前期表达量低,而后期表达量高;外源ETH处理提前了AheCS 1的表达峰值时间,整体提高了AheCS 2/3的表达量;而1-MCP处理推迟了AheCS1/2/3表达峰值出现时间,增加了AheCS1/2/3成熟后期的表达量。相关性分析发现,波罗蜜果实成熟过程中柠檬酸含量与AheCS1/2/3基因表达呈一定相关性,其中与AheCS2相关性显著。【结论】AheCS2是参与调控波罗蜜成熟过程中柠檬酸积累的潜在基因,可作为进一步研究波罗蜜AheCS基因的功能及遗传改良的候选基因。 展开更多
关键词 波罗蜜 柠檬酸 AheCS基因 克隆 基因表达
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菠萝蜜籽膳食纤维对马鲛鱼糜肌原纤维蛋白凝胶的影响
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作者 李丹 刘涛 +3 位作者 汪秀妹 周凤超 林国荣 肖意如 《保鲜与加工》 CAS 北大核心 2024年第10期74-83,共10页
为探究菠萝蜜籽可溶性膳食纤维(Jackfruit seed soluble dietary fiber,JS-SDF)对马鲛鱼肌原纤维蛋白(Myofibrillar protein,MP)凝胶性能的影响。在马鲛鱼MP中添加不同质量分数(0、0.5%、1.0%、1.5%、2.0%、2.5%)的JS-SDF,形成JS-SDF与M... 为探究菠萝蜜籽可溶性膳食纤维(Jackfruit seed soluble dietary fiber,JS-SDF)对马鲛鱼肌原纤维蛋白(Myofibrillar protein,MP)凝胶性能的影响。在马鲛鱼MP中添加不同质量分数(0、0.5%、1.0%、1.5%、2.0%、2.5%)的JS-SDF,形成JS-SDF与MP的复合体系,对MP及复合体系的内源荧光光谱、紫外吸收光谱、傅立叶红外光谱、表面疏水性等进行了分析,并测定复合体系鱼糜凝胶的凝胶强度、质构特性、微观结构等指标。结果表明:复合体系的浊度随JS-SDF添加量的增加而增大;荧光强度和凝胶白度随着JS-SDF添加量的增大而降低;除添加1.0%JS-SDF的复合体系外,其余样品紫外吸收强度均降低;红外光谱分析表明添加JS-SDF后MP的红外光谱未发生变化;表面疏水性、总巯基含量以及凝胶强度在JS-SDF添加量为1.5%时最大,同时此凝胶的持水性最高且蒸煮损失率最小,同时凝胶微观结构最为致密;十二烷基硫酸钠聚丙烯酰胺凝胶电泳(Sodium dodecyl sulfate polyacrylamidegel electrophoresis,SDS-PAGE)分析表明,JS-SDF的加入并未对肌球蛋白重链和肌动蛋白条带造成影响。因此,添加适量的JS-SDF能有效提升马鲛鱼糜的凝胶特性,改良马鲛鱼糜凝胶的品质。 展开更多
关键词 菠萝蜜籽膳食纤维 肌原纤维蛋白 凝胶特性 马鲛鱼
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云南波罗蜜园蛾类多样性及区系特征
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作者 杜浩 赵丽娟 +4 位作者 孙寅虎 岳建伟 张建春 只佳增 刘学敏 《中国南方果树》 北大核心 2024年第5期86-90,共5页
为探明云南红河流域波罗蜜园蛾类多样性及其区系特征,在河口县红河流域低热河谷区域波罗蜜园设置频振杀虫灯诱集蛾类,利用α多样性指数进行多样性分析,结合世界和中国动物地理区划进行区系特征分析。结果表明,试验地区共诱集到蛾类11科5... 为探明云南红河流域波罗蜜园蛾类多样性及其区系特征,在河口县红河流域低热河谷区域波罗蜜园设置频振杀虫灯诱集蛾类,利用α多样性指数进行多样性分析,结合世界和中国动物地理区划进行区系特征分析。结果表明,试验地区共诱集到蛾类11科57种,优势种群为青背斜纹天蛾、一点拟灯蛾、后红斜线天蛾、八点灰灯蛾、方斑拟灯蛾、星白雪灯蛾和构月天蛾;蛾类群落Shannon-Wiener多样性指数、Margale丰富度指数和Pielou均匀度指数变化趋势一致,均表现为夏季>秋季>春季>冬季;不同季节的蛾类群落Jaccard相似性指数为中等相似或极相似。在世界动物地理区划上,云南红河流域波罗蜜园蛾类主要为东洋界和古北界分布,以东洋界和古北界共有种最多,为42种;在中国动物地理区划上,7个动物地理区划中均有分布,以华南区、西南区和华中区分布为主,且西南区+华中区+华南区跨区分布物种最多,为24种。 展开更多
关键词 云南红河流域 波罗蜜 蛾类 多样性 区系
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