[Objective] This study was conducted to construct a super-high yield population of Japnica rice in cold regions of North China and to explore its characteristics. [Method] The super rice variety Longjing 21 was select...[Objective] This study was conducted to construct a super-high yield population of Japnica rice in cold regions of North China and to explore its characteristics. [Method] The super rice variety Longjing 21 was selected as the experimental material. Different row spacing(two levels), plant spacing(three levels) and seedling number per hill(three levels) were designed in field trials. Then, the growth stages,dry matter accumulation, leaf area, yield and yield components of these different treatments were measured. [Result] Rice yield had significantly negative correlation with plant spacing and row spacing, but no significant correlation with seedling number per hill. Rice yield was mainly affected by plant spacing, and less affected by seedling number per hill. The best recommended specifications for plant spacing of super rice variety Longjing 21 were 24 cm for row spacing, 12 cm for plant spacing and five seedlings per hill, and the expected yield was 10 473.0 kg/hm^2.The increased number of total spikelets(4.5×108hm2or more) in populations was the major reason for high yield. Super-high yield populations had fewer tillers at the early growth stage and achieved the expected number of productive tillers at critical leaf-age(June 25), and tiller number peaked at jointing stage(July 6) and was about 1.2 times of the expected number. The percentage of productive tillers in total tillers of super-high yield population was more than 85% at heading stage. At middle growth stage(from jointing to heading stage), the dry matter accumulation, leaf area index(LAI) at heading stage, effective leaf area, spikelet number of population,spikelet number per leaf area unit(cm2), and culm-sheath weight per stem of super-high yield population were significantly higher than those of other populations.At late growth stage(from heading to maturity stage), the leaf area decreasing rate of super-high yield population was significantly smaller than that of other populations. At late growth stage(from heading to maturity stage), the leaf area decreasing rate, crop growth rate, net assimilation rate, biomass accumulation, number of filled grains per leaf area unit(cm^2) and grain weight per leaf area unit(cm^2) of high-yield population were significantly higher than those of other populations. Output and translocation of dry matter(weight per stem and sheath and total filling rate at maturity) from heading to milky stage of super-high yield population were significantly higher than those of other populations. [Conclusion] The characteristics of superhigh yield rice in cold region of North China are enriching the amount of actual filling of sink through improving photosynthetic efficiency from heading to maturity stage on the basis of enough panicle numbers.展开更多
There is limited information about the combined effect of shading time and nitrogen (N) on grain filling and quality of rice. Therefore, two japonica super rice cultivars, Nanjing 44 and Ningjing 3, were used to stu...There is limited information about the combined effect of shading time and nitrogen (N) on grain filling and quality of rice. Therefore, two japonica super rice cultivars, Nanjing 44 and Ningjing 3, were used to study the effect of shading time and N level on the characteristics of rice panicle and grain filling as well as the corresponding yield and quality. At a low N level (150 kg N ha^-1, 150N), grain yield decreased (by 21.07-26.07%) under the treatment of 20 days of shading before heading (BH) compared with the no shading (NS) treatment. These decreases occurred because of shortened panicle length, decreased number of primary and secondary branches, as well as the grain number and weight per panicle. At 150N, in the treatment of 20 days of shading after heading (AH), grain yield also decreased (by 9.46-10.60%) due to the lower grain weight per panicle. The interaction of shading and N level had a significant effect on the number of primary and secondary branches. A high level of N (300 kg N ha^-1, 300N) could offset the negative effect of shading on the number of secondary branches and grain weight per panicle, and consequently increased the grain yield in both shading treatments. In superior grains, compared with 150N NS, the time to reach 99% of the grain weight (T99) was shortened by 1.6 to 1.7 days, and the grain weight was decreased by 4.18-5.91% in 150N BH. In 150N AH, the grain weight was 13.39-13.92% lower than that in 150N NS due to the slow mean and the maximum grain-filling rate (GRmean and GRmax). In inferior grains, grain weight and GRmean had a tendency of 150N NS〉150N BH〉150N AH. Under shaded conditions, 300N decreased the grain weight due to lower GReen both in superior and inferior grains. Compared with 150N NS, the milling and appearance qualities as well as eating and cooking quality were all decreased in 150N BH and 150N AH. Shading with the high level of 300N improved the milling quality and decreased the number of chalky rice kernels, but the eating and cooking quality was reduced with increased chalky area and overall chalkiness. Therefore, in the case of short term shading, appropriate N fertilizer could be used to improve the yield and milling quality of rice, but limited application of N fertilizer is recommended to achieve good eating and cooking quality of rice.展开更多
In this study we report the results of a decade-long breeding program for japonica super rice made by Nanjing Branch of Chinese National Center for Rice Improvement in Jiangsu Academy of Agricultural Sciences. We conc...In this study we report the results of a decade-long breeding program for japonica super rice made by Nanjing Branch of Chinese National Center for Rice Improvement in Jiangsu Academy of Agricultural Sciences. We concluded that selection of parents with good comprehensive traits and complementary advantages and disadvantages of both parents in the hybrid combination, and early selection of high heritability traits in earlier segregating generations could significantly improve the breeding efficiency. The use of closely-linked functional markers in pyramiding of multiple genes could greatly increase breeding efficiency, avoiding time-consuming and laborious steps that were used in traditional breeding program. It is also important to coordinate the yield components with variety characteristics such as yield stability, wide adaptability, lodging resistance, and an attractive grain appearance during late growth stage of rice.展开更多
In this paper, advances and prospects in breeding japonica rice for super high yield in the northern China were analyzed comprehensively in terms of breeding theories, techniques and practices. The author holds that d...In this paper, advances and prospects in breeding japonica rice for super high yield in the northern China were analyzed comprehensively in terms of breeding theories, techniques and practices. The author holds that developing and spreading super rice is an important way to enhance the overall yielding ability of japonica rice and attaining immense expansion of rice production. After theories and technical guidelines for super rice breeding were formulated, which involved the creation of new plant morphology and strong hybrid vigor through crossing indica with japonica subspecies, the optimization of combination of desirable traits via multiple crossing or backcrossing, the assemblage of favorable genes and the integration of ideal plant morphology with the utilization of vigor-major breakthroughs have been made in conventional breeding of japonica super rice. A batch of new super rice varieties marked by superior rice quality and high disease resistance, such as Shennong 265, Shennong 606, and Jijing 88, etc., have been developed and released. In comparison with the advancement in conventional breeding ef super rice, progress in hybrid japonica super rice breeding is slower because of climatic and ecological constraint in northern China. Therefore, solving the contradictions between vigor and growth duration, between yield and rice quality, and boosting vastly seed production are still serious challenges for breeders of hybrid japonica rice. Physiological and genetic problems in japonica super rice breeding are also discussed in this paper.展开更多
It is of great importance to solve the threats induced by cadmium pollution on crops. This paper examined the effect of biochar on cadmium accumulation in japonica rice and revealed the mechanism underlying the respon...It is of great importance to solve the threats induced by cadmium pollution on crops. This paper examined the effect of biochar on cadmium accumulation in japonica rice and revealed the mechanism underlying the response of protective enzyme system to cadmium stress. Biochar derived from rice straw was applied at two application rates under three cadmium concentrations. Shennong 265, super japonica rice variety, was selected as the test crop. The results indicated that cadmium content in above-ground biomass of rice increased with increasing soil cadmium concentrations, but the biochar application could suppress the accumulation of cadmium to some extent. Under high concentrations of cadmium, content of free proline and MDA (malondialdehyde) were high, so did the SOD (superoxide dismutase), POD (peroxidase) and CAT (catalase) activity in the flag leaf of rice. However, the protective enzyme activities remained at low level when biochar was added.展开更多
japonica rice is mainly distributed in Northeast China and accounts for 44.6% of the total cultivated area of japonica rice in China. The comprehensive using of inter-subspecies heterosis is the main breeding mode of ...japonica rice is mainly distributed in Northeast China and accounts for 44.6% of the total cultivated area of japonica rice in China. The comprehensive using of inter-subspecies heterosis is the main breeding mode of super japonica rice varieties in this region. Improving rice quality at relative high yielding level is the current research focus. Performing crosses between indica and japonica lines allows for the recombination of regulatory genes and genetic backgrounds, leading to complicated genetic rice quality characteristics, which can be used to explore patterns of quality improvement. In the present study, we utilize recombinant inbred lines (RILs) derived from indica-japonica hybridization to analyze the effect factors of rice quality derived from genetic factors, which contain both regulatory genes concerning rice quality and genetic backgrounds' random introduction frequency coming from indica (Di value), and the improvement strategy was further discussed. The regulatory genes involved in amylase content (Wx) and nitrogen utilization efficiency (NRT1.1B) were the major factors affecting the amylose content (AC) and protein content (PC) in RILs, respectively. Both the Di value and the major grain width gene (GS5) had regulatory effects on milled rice width (MRW) in RILs, and their interaction explained the major variance of MRW in the RILs. With the mediation of MRW and chalkiness degree (C), Di value had a further impact on head rice rate (HR), which was relatively poor when the Di value was over 40%. In Northeast China, the Di value should be lowered by backcrossing or multiple crosses during the breeding of indica-japonica hybridization to maintain the whole better HR and further to emphasize the use of favorable genes in individual selection.展开更多
[Objective] The paper was to study the expression characteristics and borer resistance of Bt gene of Songjing 9 (1C^*). [Method] With super japonica rice Songjing 9 as the receptor, transgenic crylC^* super japon...[Objective] The paper was to study the expression characteristics and borer resistance of Bt gene of Songjing 9 (1C^*). [Method] With super japonica rice Songjing 9 as the receptor, transgenic crylC^* super japonica rice Songjing 9 (1C^*) regulated by ubi promoter was created by agrobacterium-mediated genetic transformation method. Ten strains of transgenic super japonica rice formed from different transformation events were planted in the field; the Tt-mRNA of leaf, stem-sheath and young spikelet at booting stage were detected by real-time fluorescent quantitative PCR; the Bt protein content of leaf, stem-sheath and young spikelet and harvested brown rice were detected by enzyme-linked immunosorbent assay (ELISA); the white head rate damaged by Chilo suppressalis was manually investigated. [Result] The Bt-mRNA and Bt protein content of various organs and Bt protein content of mature brown rice at booting stage of Songjing 9 (1C^*) significantly differed among strains; there was no correlation relationship in Bt-mRNA and Bt protein content between leaf and stem-sheath, between leaf and young spikelet, and between stem-sheath and young spikelet; there was no relation between Bt-mRNA or Bt protein content of leaf, stem-sheath and young spikelet and Bt protein content of brown rice; there was positive correlation between Bt-mRNA and Bt protein content in the same organ such as leaf, stem-sheath and young spikelet at booting stage; such strains as Songjing 9 (1C^*), whether the Bt gene expression was high or low, showed good resistance against C.suppressalis. This indicated that there were differences in Bt gene expression in different strains of transgenic cry1C^* super japonica rice or different organs of the same strain; there was no relation in Bt gene expression among different organs; the Bt-mRNA and protein content of the same organ usually had consistent expression; the Bt gene expression of various organs had nothing to do with the Bt protein content of brown rice. [Conclusion] The Bt gene and its expression cassette can meet the requirement of cultivating japonica rice varieties against stem borers.展开更多
[ Objective ] The paper was to study the density restriction effects of reproductive rate of rice brown planthopper (BPH) population in super indiea and japonica hybrid rice Yongyou 6. [Method] Different amounts of ...[ Objective ] The paper was to study the density restriction effects of reproductive rate of rice brown planthopper (BPH) population in super indiea and japonica hybrid rice Yongyou 6. [Method] Different amounts of rice brown planthoppor were released to super indiea and japonica hybrid flee Yongyou 6 during fillering, booting and gain filling stage, respectively. The reproductive rate and variation dynamics of rice brown planthoppor in super rice was also investigated and analyzed. [Result] Tillering stage: the population quantity of rice brown planthoppor during booting and gain filling stage gradually increased with the increasing basal amount of its population during tillering stage; when the population density of rice brown planthopper during grain filling stage reached a certain limit ( 〉 250 head/cluster), the population density during milking stage showed decrease trend with the increasing of such density; the development of rice brown planthoppor population in super rice Yongyou 6 showed the characteristics of fast in middle and slow in two ends. Booting stage: the development of rice brown planthopper pop- ulation from booting to gain filling stage increased with the increasing basal amount of density, and their correlation during milking stage was not significant; flee brown planthopper population still had high reproduction capacity in the middle and late stage of super rice, which had latent risk on rice yield. Gain filling stage : when population density of rice brown planthopper 〈 70 head/cluster, the development of it population increased with the increase of basal amount of density, which had great threat to yield. [ Conclusion] The result provided theoretical basis for establishment and improvement of monitoring early warning system, cost-ef- fective control of occurrence and damage of rice brown planthopper, and improvement of the sustained control ability against rice brown planthopper.展开更多
Chunyou 284 is a medium- japonica hybrid combination,as well as an indica-japonica subspecific hyrbid with super-high yield,which was developed from Chunjiang 23A,a thermo-sensitive CMS line with very early anthesis t...Chunyou 284 is a medium- japonica hybrid combination,as well as an indica-japonica subspecific hyrbid with super-high yield,which was developed from Chunjiang 23A,a thermo-sensitive CMS line with very early anthesis time,and C84,an indica-japonica intermediate type restorer line with wide compatibility.This combination has the advantages of high yield potential,early maturity,excellent comprehensive agronomic traits and wide adaptability.It was approved by Jiangsu Provincial Crop Variety Approval Committee in June,2018.The breeding process,main characteristics,cultivation techniques and seed production points of the combination were introduced.展开更多
大田条件下以超级粳稻南粳44和宁粳3号为材料,设置2种氮肥水平(N10:150 kg hm–2,N20:300 kg hm–2)和3种遮光处理(L1:不遮光,L2:抽穗前遮光20 d,L3:抽穗后遮光20 d),研究光、氮及其互作对超级粳稻产量和物质生产特征的影响。...大田条件下以超级粳稻南粳44和宁粳3号为材料,设置2种氮肥水平(N10:150 kg hm–2,N20:300 kg hm–2)和3种遮光处理(L1:不遮光,L2:抽穗前遮光20 d,L3:抽穗后遮光20 d),研究光、氮及其互作对超级粳稻产量和物质生产特征的影响。结果表明,较之L1,L2不仅减少了有效穗数和每穗粒数,导致总颖花量下降,降幅达24.81%-35.63%,而且还显著降低了抽穗期茎蘖数和叶面积指数,降幅达2.90%-6.44%和19.02%-27.17%,导致抽穗至成熟阶段的光合势、干物质积累量显著下降,最终产量显著下降,降幅达27.23%-35.26%。较之L1,L3主要影响了抽穗至成熟阶段的光合物质积累,导致结实率和千粒重显著下降,降幅达1.49%-4.48%和5.54%-9.17%,最终产量显著下降,降幅达10.91%-18.47%。L2条件下,随着氮肥水平增加,抽穗期茎蘖数与叶面积指数均显著增加,导致抽穗至成熟阶段光合势、干物质积累显著增加,最终有效穗数、每穗粒数、总颖花量以及产量显著提高。L3条件下,随着氮肥水平增加,抽穗至成熟阶段的光合物质积累显著提高,其中茎叶干物质向穗部转运量显著增加,转运率和贡献率也进一步提高,最终产量显著提高。由此可见氮肥施用能部分弥补因弱光逆境对超级粳稻物质生产及其产量的影响。展开更多
在高产施氮量条件下(225 kg hm-2),按基蘖肥、穗肥不同施氮比例且穗肥依不同叶龄期追氮共设计了20种施氮模式,研究了其对超级杂交粳稻常优1号产量、氮素利用率及主要米质性状的影响。结果表明:氮肥运筹对常优1号产量影响极大,基蘖肥与...在高产施氮量条件下(225 kg hm-2),按基蘖肥、穗肥不同施氮比例且穗肥依不同叶龄期追氮共设计了20种施氮模式,研究了其对超级杂交粳稻常优1号产量、氮素利用率及主要米质性状的影响。结果表明:氮肥运筹对常优1号产量影响极大,基蘖肥与穗肥施氮比例为58.34∶41.66,穗肥以叶龄余数4、2叶等量施氮时,产量最高;产量与总吸氮量、氮肥农学利用率、氮肥表观利用率、生理利用率呈极显著正相关关系,氮收获指数和百千克籽粒需氮量与产量呈二次曲线关系;随着穗肥施氮比例的下降,稻米整精米率、蛋白质含量、垩白率、垩白度逐渐下降;胶稠度、直链淀粉含量呈直线上升趋势;穗肥随追肥叶龄期推迟,整精米率、蛋白质含量提高,而直链淀粉含量下降。胶稠度、外观品质因基蘖肥施氮比例的不同而异。综合来看,基蘖肥、穗肥施氮比例为6∶4,穗肥以倒4、2叶期追肥可使该品种超高产、优质、高效得到较好的协调统一。展开更多
基金Supported by National Key Technology Research and Development Program of China(2011BAD16B11-02YJ01,2012BAD04B01-02)Key Science and Technology Program of Heilongjiang Province+2 种基金China(GA13B101)Heilongjiang Postdoctoral Sustentation Fund(LBH-Z10038)the Funds of Heilongjiang Academy of Agricultural Sciences for Distinguished Young Scholars(2014)~~
文摘[Objective] This study was conducted to construct a super-high yield population of Japnica rice in cold regions of North China and to explore its characteristics. [Method] The super rice variety Longjing 21 was selected as the experimental material. Different row spacing(two levels), plant spacing(three levels) and seedling number per hill(three levels) were designed in field trials. Then, the growth stages,dry matter accumulation, leaf area, yield and yield components of these different treatments were measured. [Result] Rice yield had significantly negative correlation with plant spacing and row spacing, but no significant correlation with seedling number per hill. Rice yield was mainly affected by plant spacing, and less affected by seedling number per hill. The best recommended specifications for plant spacing of super rice variety Longjing 21 were 24 cm for row spacing, 12 cm for plant spacing and five seedlings per hill, and the expected yield was 10 473.0 kg/hm^2.The increased number of total spikelets(4.5×108hm2or more) in populations was the major reason for high yield. Super-high yield populations had fewer tillers at the early growth stage and achieved the expected number of productive tillers at critical leaf-age(June 25), and tiller number peaked at jointing stage(July 6) and was about 1.2 times of the expected number. The percentage of productive tillers in total tillers of super-high yield population was more than 85% at heading stage. At middle growth stage(from jointing to heading stage), the dry matter accumulation, leaf area index(LAI) at heading stage, effective leaf area, spikelet number of population,spikelet number per leaf area unit(cm2), and culm-sheath weight per stem of super-high yield population were significantly higher than those of other populations.At late growth stage(from heading to maturity stage), the leaf area decreasing rate of super-high yield population was significantly smaller than that of other populations. At late growth stage(from heading to maturity stage), the leaf area decreasing rate, crop growth rate, net assimilation rate, biomass accumulation, number of filled grains per leaf area unit(cm^2) and grain weight per leaf area unit(cm^2) of high-yield population were significantly higher than those of other populations. Output and translocation of dry matter(weight per stem and sheath and total filling rate at maturity) from heading to milky stage of super-high yield population were significantly higher than those of other populations. [Conclusion] The characteristics of superhigh yield rice in cold region of North China are enriching the amount of actual filling of sink through improving photosynthetic efficiency from heading to maturity stage on the basis of enough panicle numbers.
基金grants from the National Key Technology R&D Program of China (2016YFD0300503)the Key Research Program of Jiangsu Province, China (BE2016344)+3 种基金the earmarked fund for China Agriculture Research System (CARS-01-27)the National Nature Science Foundation of China (31701350)the Program for Scientific Elitists of Yangzhou University, Chinafunded by the Priority Academic Program Development of Jiangsu Higher Education Institutions,China
文摘There is limited information about the combined effect of shading time and nitrogen (N) on grain filling and quality of rice. Therefore, two japonica super rice cultivars, Nanjing 44 and Ningjing 3, were used to study the effect of shading time and N level on the characteristics of rice panicle and grain filling as well as the corresponding yield and quality. At a low N level (150 kg N ha^-1, 150N), grain yield decreased (by 21.07-26.07%) under the treatment of 20 days of shading before heading (BH) compared with the no shading (NS) treatment. These decreases occurred because of shortened panicle length, decreased number of primary and secondary branches, as well as the grain number and weight per panicle. At 150N, in the treatment of 20 days of shading after heading (AH), grain yield also decreased (by 9.46-10.60%) due to the lower grain weight per panicle. The interaction of shading and N level had a significant effect on the number of primary and secondary branches. A high level of N (300 kg N ha^-1, 300N) could offset the negative effect of shading on the number of secondary branches and grain weight per panicle, and consequently increased the grain yield in both shading treatments. In superior grains, compared with 150N NS, the time to reach 99% of the grain weight (T99) was shortened by 1.6 to 1.7 days, and the grain weight was decreased by 4.18-5.91% in 150N BH. In 150N AH, the grain weight was 13.39-13.92% lower than that in 150N NS due to the slow mean and the maximum grain-filling rate (GRmean and GRmax). In inferior grains, grain weight and GRmean had a tendency of 150N NS〉150N BH〉150N AH. Under shaded conditions, 300N decreased the grain weight due to lower GReen both in superior and inferior grains. Compared with 150N NS, the milling and appearance qualities as well as eating and cooking quality were all decreased in 150N BH and 150N AH. Shading with the high level of 300N improved the milling quality and decreased the number of chalky rice kernels, but the eating and cooking quality was reduced with increased chalky area and overall chalkiness. Therefore, in the case of short term shading, appropriate N fertilizer could be used to improve the yield and milling quality of rice, but limited application of N fertilizer is recommended to achieve good eating and cooking quality of rice.
基金supported by the National Key Technologies R&D Program of China during the 12th Five-Year Plan period(2015BAD01B02)the Special Program for Rice Scientific Research,Ministry of Agriculture,China(201603002-5-1)the Construction of Modern Agricultural and Industrial Technology System Project special fund,China(CARS01-47)
文摘In this study we report the results of a decade-long breeding program for japonica super rice made by Nanjing Branch of Chinese National Center for Rice Improvement in Jiangsu Academy of Agricultural Sciences. We concluded that selection of parents with good comprehensive traits and complementary advantages and disadvantages of both parents in the hybrid combination, and early selection of high heritability traits in earlier segregating generations could significantly improve the breeding efficiency. The use of closely-linked functional markers in pyramiding of multiple genes could greatly increase breeding efficiency, avoiding time-consuming and laborious steps that were used in traditional breeding program. It is also important to coordinate the yield components with variety characteristics such as yield stability, wide adaptability, lodging resistance, and an attractive grain appearance during late growth stage of rice.
文摘In this paper, advances and prospects in breeding japonica rice for super high yield in the northern China were analyzed comprehensively in terms of breeding theories, techniques and practices. The author holds that developing and spreading super rice is an important way to enhance the overall yielding ability of japonica rice and attaining immense expansion of rice production. After theories and technical guidelines for super rice breeding were formulated, which involved the creation of new plant morphology and strong hybrid vigor through crossing indica with japonica subspecies, the optimization of combination of desirable traits via multiple crossing or backcrossing, the assemblage of favorable genes and the integration of ideal plant morphology with the utilization of vigor-major breakthroughs have been made in conventional breeding of japonica super rice. A batch of new super rice varieties marked by superior rice quality and high disease resistance, such as Shennong 265, Shennong 606, and Jijing 88, etc., have been developed and released. In comparison with the advancement in conventional breeding ef super rice, progress in hybrid japonica super rice breeding is slower because of climatic and ecological constraint in northern China. Therefore, solving the contradictions between vigor and growth duration, between yield and rice quality, and boosting vastly seed production are still serious challenges for breeders of hybrid japonica rice. Physiological and genetic problems in japonica super rice breeding are also discussed in this paper.
基金supported by the National Natural Science Foundation of China (41201317)the Key Technologies R&D Program of China during the 12th Five-Year Plan period (2012BAD14B01)
文摘It is of great importance to solve the threats induced by cadmium pollution on crops. This paper examined the effect of biochar on cadmium accumulation in japonica rice and revealed the mechanism underlying the response of protective enzyme system to cadmium stress. Biochar derived from rice straw was applied at two application rates under three cadmium concentrations. Shennong 265, super japonica rice variety, was selected as the test crop. The results indicated that cadmium content in above-ground biomass of rice increased with increasing soil cadmium concentrations, but the biochar application could suppress the accumulation of cadmium to some extent. Under high concentrations of cadmium, content of free proline and MDA (malondialdehyde) were high, so did the SOD (superoxide dismutase), POD (peroxidase) and CAT (catalase) activity in the flag leaf of rice. However, the protective enzyme activities remained at low level when biochar was added.
基金sponsored by the National Natural Science Foundation of China(31371587,31430062)the Cultivation Plan for Youth Agricultural Science Technology Innovative Talents of Liaoning Province,China(2015035,2015036)the Program for Changjiang Scholars and Innovative Research Team in University,China(IRT13079)
文摘japonica rice is mainly distributed in Northeast China and accounts for 44.6% of the total cultivated area of japonica rice in China. The comprehensive using of inter-subspecies heterosis is the main breeding mode of super japonica rice varieties in this region. Improving rice quality at relative high yielding level is the current research focus. Performing crosses between indica and japonica lines allows for the recombination of regulatory genes and genetic backgrounds, leading to complicated genetic rice quality characteristics, which can be used to explore patterns of quality improvement. In the present study, we utilize recombinant inbred lines (RILs) derived from indica-japonica hybridization to analyze the effect factors of rice quality derived from genetic factors, which contain both regulatory genes concerning rice quality and genetic backgrounds' random introduction frequency coming from indica (Di value), and the improvement strategy was further discussed. The regulatory genes involved in amylase content (Wx) and nitrogen utilization efficiency (NRT1.1B) were the major factors affecting the amylose content (AC) and protein content (PC) in RILs, respectively. Both the Di value and the major grain width gene (GS5) had regulatory effects on milled rice width (MRW) in RILs, and their interaction explained the major variance of MRW in the RILs. With the mediation of MRW and chalkiness degree (C), Di value had a further impact on head rice rate (HR), which was relatively poor when the Di value was over 40%. In Northeast China, the Di value should be lowered by backcrossing or multiple crosses during the breeding of indica-japonica hybridization to maintain the whole better HR and further to emphasize the use of favorable genes in individual selection.
基金Supported by National High Technology Research and Development Program(863 plan)of China(2014AA10A600)National Major Project for Transgenic Organism Breeding of China(2016ZX08001001-001-007)Open Project of Key Laboratory of Molecular Biology in Colleges and Universities of Heilongjiang Province(1206)
文摘[Objective] The paper was to study the expression characteristics and borer resistance of Bt gene of Songjing 9 (1C^*). [Method] With super japonica rice Songjing 9 as the receptor, transgenic crylC^* super japonica rice Songjing 9 (1C^*) regulated by ubi promoter was created by agrobacterium-mediated genetic transformation method. Ten strains of transgenic super japonica rice formed from different transformation events were planted in the field; the Tt-mRNA of leaf, stem-sheath and young spikelet at booting stage were detected by real-time fluorescent quantitative PCR; the Bt protein content of leaf, stem-sheath and young spikelet and harvested brown rice were detected by enzyme-linked immunosorbent assay (ELISA); the white head rate damaged by Chilo suppressalis was manually investigated. [Result] The Bt-mRNA and Bt protein content of various organs and Bt protein content of mature brown rice at booting stage of Songjing 9 (1C^*) significantly differed among strains; there was no correlation relationship in Bt-mRNA and Bt protein content between leaf and stem-sheath, between leaf and young spikelet, and between stem-sheath and young spikelet; there was no relation between Bt-mRNA or Bt protein content of leaf, stem-sheath and young spikelet and Bt protein content of brown rice; there was positive correlation between Bt-mRNA and Bt protein content in the same organ such as leaf, stem-sheath and young spikelet at booting stage; such strains as Songjing 9 (1C^*), whether the Bt gene expression was high or low, showed good resistance against C.suppressalis. This indicated that there were differences in Bt gene expression in different strains of transgenic cry1C^* super japonica rice or different organs of the same strain; there was no relation in Bt gene expression among different organs; the Bt-mRNA and protein content of the same organ usually had consistent expression; the Bt gene expression of various organs had nothing to do with the Bt protein content of brown rice. [Conclusion] The Bt gene and its expression cassette can meet the requirement of cultivating japonica rice varieties against stem borers.
文摘[ Objective ] The paper was to study the density restriction effects of reproductive rate of rice brown planthopper (BPH) population in super indiea and japonica hybrid rice Yongyou 6. [Method] Different amounts of rice brown planthoppor were released to super indiea and japonica hybrid flee Yongyou 6 during fillering, booting and gain filling stage, respectively. The reproductive rate and variation dynamics of rice brown planthoppor in super rice was also investigated and analyzed. [Result] Tillering stage: the population quantity of rice brown planthoppor during booting and gain filling stage gradually increased with the increasing basal amount of its population during tillering stage; when the population density of rice brown planthopper during grain filling stage reached a certain limit ( 〉 250 head/cluster), the population density during milking stage showed decrease trend with the increasing of such density; the development of rice brown planthoppor population in super rice Yongyou 6 showed the characteristics of fast in middle and slow in two ends. Booting stage: the development of rice brown planthopper pop- ulation from booting to gain filling stage increased with the increasing basal amount of density, and their correlation during milking stage was not significant; flee brown planthopper population still had high reproduction capacity in the middle and late stage of super rice, which had latent risk on rice yield. Gain filling stage : when population density of rice brown planthopper 〈 70 head/cluster, the development of it population increased with the increase of basal amount of density, which had great threat to yield. [ Conclusion] The result provided theoretical basis for establishment and improvement of monitoring early warning system, cost-ef- fective control of occurrence and damage of rice brown planthopper, and improvement of the sustained control ability against rice brown planthopper.
基金Supported by National Key R&D Program of China(2017YFD0100405)the Agricultural Science and Technology Innovation Program of Chinese Academy of Agricultural Sciences(CAAS-ASTIP-201X-CNRRI)+3 种基金Independent Research Project of State Key Laboratory of Rice Biology(2017ZZKT10204)the Major Scientific Technological Project of New Varieties of Agriculture(Grain)of Zhejiang Province(2016C02050-5-1)the Fundamental Research Fund for China National Rice Research Institute(2017RG003-1)Breeding of High-yield Genetically Modified Rice Varieties(2016ZX08001004-001)
文摘Chunyou 284 is a medium- japonica hybrid combination,as well as an indica-japonica subspecific hyrbid with super-high yield,which was developed from Chunjiang 23A,a thermo-sensitive CMS line with very early anthesis time,and C84,an indica-japonica intermediate type restorer line with wide compatibility.This combination has the advantages of high yield potential,early maturity,excellent comprehensive agronomic traits and wide adaptability.It was approved by Jiangsu Provincial Crop Variety Approval Committee in June,2018.The breeding process,main characteristics,cultivation techniques and seed production points of the combination were introduced.
文摘大田条件下以超级粳稻南粳44和宁粳3号为材料,设置2种氮肥水平(N10:150 kg hm–2,N20:300 kg hm–2)和3种遮光处理(L1:不遮光,L2:抽穗前遮光20 d,L3:抽穗后遮光20 d),研究光、氮及其互作对超级粳稻产量和物质生产特征的影响。结果表明,较之L1,L2不仅减少了有效穗数和每穗粒数,导致总颖花量下降,降幅达24.81%-35.63%,而且还显著降低了抽穗期茎蘖数和叶面积指数,降幅达2.90%-6.44%和19.02%-27.17%,导致抽穗至成熟阶段的光合势、干物质积累量显著下降,最终产量显著下降,降幅达27.23%-35.26%。较之L1,L3主要影响了抽穗至成熟阶段的光合物质积累,导致结实率和千粒重显著下降,降幅达1.49%-4.48%和5.54%-9.17%,最终产量显著下降,降幅达10.91%-18.47%。L2条件下,随着氮肥水平增加,抽穗期茎蘖数与叶面积指数均显著增加,导致抽穗至成熟阶段光合势、干物质积累显著增加,最终有效穗数、每穗粒数、总颖花量以及产量显著提高。L3条件下,随着氮肥水平增加,抽穗至成熟阶段的光合物质积累显著提高,其中茎叶干物质向穗部转运量显著增加,转运率和贡献率也进一步提高,最终产量显著提高。由此可见氮肥施用能部分弥补因弱光逆境对超级粳稻物质生产及其产量的影响。
文摘在高产施氮量条件下(225 kg hm-2),按基蘖肥、穗肥不同施氮比例且穗肥依不同叶龄期追氮共设计了20种施氮模式,研究了其对超级杂交粳稻常优1号产量、氮素利用率及主要米质性状的影响。结果表明:氮肥运筹对常优1号产量影响极大,基蘖肥与穗肥施氮比例为58.34∶41.66,穗肥以叶龄余数4、2叶等量施氮时,产量最高;产量与总吸氮量、氮肥农学利用率、氮肥表观利用率、生理利用率呈极显著正相关关系,氮收获指数和百千克籽粒需氮量与产量呈二次曲线关系;随着穗肥施氮比例的下降,稻米整精米率、蛋白质含量、垩白率、垩白度逐渐下降;胶稠度、直链淀粉含量呈直线上升趋势;穗肥随追肥叶龄期推迟,整精米率、蛋白质含量提高,而直链淀粉含量下降。胶稠度、外观品质因基蘖肥施氮比例的不同而异。综合来看,基蘖肥、穗肥施氮比例为6∶4,穗肥以倒4、2叶期追肥可使该品种超高产、优质、高效得到较好的协调统一。