On the bases of archaeological discoveries, the earliest domestication of rice has been confirmed in the middle and lower Changjiang River basin, while in the region wild rice populations are found in shallow swamps u...On the bases of archaeological discoveries, the earliest domestication of rice has been confirmed in the middle and lower Changjiang River basin, while in the region wild rice populations are found in shallow swamps under a climate with freezing winter cold. These findings lead us to-examine the past ideas about domestication and differentiation of rice. Historically, in 1930s two sub-species, indica and japonica, were proposed on the basis of sterility in F1 hybrids between them. Soon after that, the two types were classified by the associations of a number of genetically independent traits. The characteristic associations of traits have been explained by the hybrid sterility or reproductive barriers which were assumed to comprise a set of duplicate recessive lethal genes and to be an inner genetic mechanism to lead to the varietal differentiations In 1980s, the hybrid sterility between Indica and Japonica types was analyzed, and Indica, Japonica, and wide-compatibility type which gives fertile hybrids when cross to Indica and Japonica types, are proved to contain an allele, S5', S5' and S5^n, respectively at a locus on chromosome 6. And those gametes having Sj allele are found to be partially aborted in the hybrid genotypes of S5'/S5' while no gamete abortion occurs in S5'/S5^n and S5'/S5^n genotypes. Since then, the gene S5^n has been used in hybrid rice breeding to obtain fertile and vigorous hybrids between subspecies, and the long-disputed problem of hybrid sterility has been solved. Also in such studies the characteristic association of traits found in each of vadetal groups is better explained by founder effects. On the other hand, a large number of native cultivars of rice were surveyed with enzyme polymorphism in 1980s and later with molecular markers. As a result, profound genetic diversity is found in cultivated rice as well as in wild rice. These findings seem to lead us to the idea of multiple independent domestications of rice. However, before reaching such a conclusion, at least two factors, i.e., long-distance-dissemination of some genotypes and the possibilities of introgression by local wild rice to primitive cultivars need to be examined. Taking the two factors as well as the historical events into consideration, it is considered here that the perennial japonica cultivars which are close to wild rice in the Changjiang River basin were disseminated to East India through Assam or along the Bengal Bay, where they were transformed under the introgressions of local wild rice and formed a secondary center, from which some genotypes seem to be disseminated to colonies in Southeast Asia under the influence of Hinduism. Later some of the genotypes were introduced into China and constituted so called Indica type. This may be a reasonable picture for the varietal differentiation.展开更多
Plant type and grain quality are two major aspects in rice breeding. Using canonical correlation analysis and canonical redundancy analysis, the relationship between plant type traits and rice grain quality traits was...Plant type and grain quality are two major aspects in rice breeding. Using canonical correlation analysis and canonical redundancy analysis, the relationship between plant type traits and rice grain quality traits was studied with 100 crosses derived from 10 sterile lines × 10 restorer lines. There was a complex relationship between parts of the traits of the two aspects. The angle of the 2nd leaf from the top and single panicle weight played important roles in plant type system and amylose content and grain length in grain quality system. The angle of the 2nd leaf from the top, plant height and single panicle weight had a great effect on grain quality traits, and amylose content, brown rice rate and translucency were easily influenced by plant type traits. Selection index model indicated that japonica hybrid rice in Northern China with good quality was characterized by broad flag leaf and 2nd leaf from the top, narrow and short 3rd leaf from the top, low plant height, short culm, long and more panicles and low single panicle weight.展开更多
The relationship between the indica-japonica differentiation of parents and heterosis of hybrid in rice was studied with 10 sterile lines, 10 maintainer lines of the sterile lines, 38 restorer lines of Dian type hybri...The relationship between the indica-japonica differentiation of parents and heterosis of hybrid in rice was studied with 10 sterile lines, 10 maintainer lines of the sterile lines, 38 restorer lines of Dian type hybrid rice and 40 hybrids derived from the sterile lines and restorer lines. The results indicated that there was a parabolic correlation between the indica-japonica differentiation of parents detected by RAPD markers and heterosis of hybrid rice. When certain indica-japonica differentiation of parents exists, the percentage of combinations with positve heterosis and the mean heterosis of hybrids were high. But that didn't mean too much difference of parents could lead to stronger hetesosis, that meant overdiffernt parents seldom bring positve het-erotic hybrids. Suitable difference of percent of indica alleles of parents of stronger heterotic hybrid was 12 -16%.展开更多
This study was designed to analyze the genetic relationship of the morphological traits of the northern japonica hybrid rice. The genetic relationship of the morphological traits of the 100 cultivars combined by the 1...This study was designed to analyze the genetic relationship of the morphological traits of the northern japonica hybrid rice. The genetic relationship of the morphological traits of the 100 cultivars combined by the 10 sterile lines and the 10 restore lines was studied by using the principle component analysis and the SAS and QGA. The results showed that compared with the female parent, most of the morphological traits related closely to their father. The additive genetic effect and the dominant genetic effect appeared significantly, and the former was more significant than the latter. Genetic correlation analysis indicated that high plants or high plants with more leaves were in favor of improving the number and the weight of the spike and producing the hybrid vigor. The important factors influencing the plant type of the northern japonica hybrid rice were the plant height (PH), the angle of the flag leaf (FLA), flag leaf length (FLL), and the weight of the shoots and leaves (SLW), respectively. The morphological traits system of the northern japonica hybrid rice was very complex, and the restore line should be given more importance compared with the sterile line, and PH, FLA, FLL, and SLW should be considered first in the plant type breeding.展开更多
Under field conditions, three erect panicle type japonica dee cultivars and three curved panicle type japonica rice cultivars were applied as experimental materials to investigate the differences of protein compositio...Under field conditions, three erect panicle type japonica dee cultivars and three curved panicle type japonica rice cultivars were applied as experimental materials to investigate the differences of protein composition of grains at different positions in panicles between two different panicle types of japonica rice. Accord- ing to the results, the panicle type of japonica rice had no direct correlation with albumin content, globulin content, prolamin content and glutelin content in rice, variations of protein composition of grains at different positions in panicles. There were certain correlations between soluble protein contents at different grain posi- tions in the same panicle and the flowering order of glumous flowers in the panicle. Albumin content, prolamin content and glutelin content in grains on secondary rachis branches of two panicle types (erect and curved) of japonica rice cuhivars were higher than that on primary rachis branches, while globulin content exhibited an opposite trend. Globulin content, prolamin content and glutelin content in grains at different positions demonstrated a descending order of bottom 〉 middle 〉 top, while albumin content exhibited an opposite trend. The interactions between primary and secondary rachis branches and among top, middle and bottom rachis branches significantly affected soluble protein contents.展开更多
Two recombinant inbred line (RIL) populations, one derived from a cross between Zhongyouzao 8 (indica) and Toyonishiki (japonica) and the other from a cross between Qishanzhan (indica) and Akihikari (japonica...Two recombinant inbred line (RIL) populations, one derived from a cross between Zhongyouzao 8 (indica) and Toyonishiki (japonica) and the other from a cross between Qishanzhan (indica) and Akihikari (japonica), were grown in Liaoning and Sichuan Provinces, China, to study the panicle type index (PTI, the ratio of a number of node position on panicle axis where the secondary branch with the most numerous secondary branch grains is located to the number of primary branches) and its relationships with subspecies characteristics and yield traits. With continuous distribution of PTI and numerous of lines with different PTIs in the progenies, indica-japonica hybridization could be a method for breeding cultivars with ideal panicle type. PTI was significantly influenced by environments, with a significant decrease from Liaoning to Sichuan A significantly negative correlation was found between PTI and most of subspecies characteristics. PTI varied remarkably with different subspecies types. As a whole, it showed a trend of indica (H)〉indicalinous (H')〉japonicalinous (K')〉japonica (K) However, it is not appropriate to regard PTI as a parameter for subspecies classification in rice because it was recombined in the filial generations of cross between indica and japonica rice. The negative correlations were found between PTI and most of panicle characteristics, as well as yield-related traits. Based on PTI, most of lines from the two populations were middle dominant panicle type (the number of grains on the secondary branch is numerous in middle position of the panicle) and lower dominant panicle type (the number of grains on the secondary branch is numerous in lower position of the panicle and becomes less towards the top of panicle) with significant yield advantages.展开更多
Late-maturity type of Yongyou japonica/indica hybrids series (LMYS) have shown great yield potential, and are being widely planted in the lower reaches of Yangtze River, China. Knowledge about suitable growing zone ...Late-maturity type of Yongyou japonica/indica hybrids series (LMYS) have shown great yield potential, and are being widely planted in the lower reaches of Yangtze River, China. Knowledge about suitable growing zone and evaluation of yield advantage is of practicall importance for LMYS in this region. Fifteen LMYS, two high-yielding inbred japonica check varieties (CK-J) and two high-yielding hybrid indica check varieties (CK-I) were grown at Xinghua (119.57°E, 33.05°N) of Lixiahe region, Yangzhou (119.25°E, 32.30°N)of Yanjiang region, Changshu (120.46°E, 31.41°N)of Taihu Lake region, and Ningbo (121.31°E, 29.45°N) of Ningshao Plain in 2013 and 2014. The results showed that maturity dates of the 15 were later than the secure maturity date at Xinghua and 6, 14 and 15 LMYS were mature before the secure maturity date at Yangzhou, Changshu and Ningbo, respectively. One variety was identified as high-yielding variety among LMYS (HYYS) at Yangzhou, 8 HYYS in 201:3 and 9 HYYS in 2014 at Changshu, 9 HYYS at Ningbo. HYYS here referred to the variety among LMYS that was mature before the secure maturity date and had at least 8% higher grain yield than both CK-J and CK-I at each experimental site. Grain yield of HYYS at each experimental site was about 12.0 t ha-1 or higher, and was significantly higher than CK varieties. High yield of HYYS was mainly attributed to larger sink size due to more spikelets per panicle. Plant height of HYYS was about 140 cm, and was significantly higher than check varieties. Significant positive correlations were recorded between duration from heading to maturity stage and grain yield, and also between whole growth periods and grain yield. HYYS had obvious advantage over check varieties in biomass accumulation and leaf area duration from heading to maturity stage. Comprehensive consideration about safe matudty and yield performance of LMYS at each experimental site, Taihu Lake region (representative site Changshu) and Ningshao Plain (representative site Ningbo) were thought suitable growing zones for LMYS in the lower reaches of Yangtze River. The main factors underlying high yield ofHYYS were larger sink size, higher plant height, longer duration from heading to maturity stage and whole growth periods, and higher biomass accumulation and leaf area duration during grain filling stage.展开更多
BACKGROUND: It has been shown that interleukin-1β(IL-1β) can induce fever by activating vascular endothelial cells and macrophages of the supraoptic crest to generate prostaglandin E2, which binds with receptors ...BACKGROUND: It has been shown that interleukin-1β(IL-1β) can induce fever by activating vascular endothelial cells and macrophages of the supraoptic crest to generate prostaglandin E2, which binds with receptors of the thermo-sensitive hypothalamic neurons. Lonicera japonica is one of the medicinal plants used widely in Asia for its antipyretic properties. However, these mechanisms have not yet been intensively studied. OBJECTIVE: To investigate the antipyretic effect and mechanisms of Lonicera japonica on IL-1β- induced febrile New Zealand rabbits by observing expression changes of E-type prostaglandin receptor-3 (EP3) mRNA in the preoptic anterior hypothalamus (POAH). DESIGN: A randomized controlled study. SETTING: Electrophysiological Laboratory at the Department of Pathophysiology, Medical College of Jinan University; Department of Orthopaedics, First Hospital Affiliated to Medical College of Jinan University. MATERIALS: The experiment was performed from April to December 2005, using a total of 32 New Zealand white rabbits of both sexes, weighing 1.5 2.0 kg. All the animal experiments were performed according to the internationally accepted ethical guidelines. Lonicera japonica injection was purchased from Huanghe pharmaceutical factory of Xi'an, China. IL-1βwas purchased from Sigma, USA. METHODS: A total of 32 rabbits were divided randomly into four groups: ① Normal saline (NS) control group;② Lonicerajaponica treatment group; ③ IL-1βtreatment group; and ④Lonicerajaponica plus IL-1βtreatment group. In the first 3 groups, the rabbits were given separate intravenous (i.v.) injections of l mL NS, l mL Lonicera japonica, and 100 ng IL-l β (dissolved in 0.9% NS without pyrogen). In the Lonicerajaponica plus IL-1βgroup each rabbit was given i.v. injections of l mL NS and, 30 minutes later, 100 ng IL-1 β. MAIN OUTCOME MEASURES: Colonic temperature of each rabbit was measured at 0, 10, 20, 30, 40, 50, 60, and 70 minutes after injection and the maximum temperature rise ( A T) and the temperature response index after l hour (TRII) was calculated. Subsequently, in situ hybridization (ISH) was done with an ISH kit, EP3 mRNA expression in the POAH of all groups was measured (number of positive cells and average gray scale value). RESULTS: A total of 32 rabbits were included in the result analysis, without any loss, (i) A T and TRII: there was no significant difference between the Lonicera japonica group and NS group (P 〉 0.05). The IL-1β group was significantly greater compared to NS group (P 〈 0.01). The Lonicera japonica plus IL-1β group was significantly less than the IL-1β group (P 〈 0.05). ② In the NS and Lonicera japonica groups, the EP3 mRNA expression was negative (no coloration) or only weakly positive (only a few brown yellow particles in the cytoplasm cells could not be identified). The number of positive cells and the average gray scale value were not significantly different between the two groups (P 〉 0.05). In the IL-1β group, the number of positive cells was remarkably higher and the average gray scale value was lower than the NS group (P 〈 0.0 l). In the Lonicera japonica plus IL-1β group, the number of positive cells was significantly less than the IL-1β group (P 〈 0.05). However, the average gray scale value was greater than the IL-1β group (P 〈 0.05). CONCLUSION: Lonicera japonica has obvious antipyretic effects on IL-1β-induced febrile rabbits and acts by inhibiting expression of EP3 mRNA in the POAH.展开更多
The yield potential of japonica/indica hybrids(JIH)has been achieved over 13.5 t ha–1 in large-scale rice fields,and some physiological traits for yield advantage of JIH over japonica inbred rice(JI)and indica hybrid...The yield potential of japonica/indica hybrids(JIH)has been achieved over 13.5 t ha–1 in large-scale rice fields,and some physiological traits for yield advantage of JIH over japonica inbred rice(JI)and indica hybrid rice(IH)were also identified.To date,little attention has been paid to morphological traits for yield advantage of JIH over JI and IH.For this reason,three JIH,three JI,and three IH were field-grown at East China(Ningbo,Zhejiang Province)in 2015 and 2016.Compared with JI and IH,JIH had 14.3 and 20.8%higher grain yield,respectively,attributed to its more spikelets per panicle and relatively high percentage of filled grains.The advantage in spikelets per panicle of JIH over JI and IH was shown in number of grains on the upper,middle,and lower branches.Compared with JI and IH,JIH had higher leaf area through leaf width and lower leaf angle of upper three leaves,higher leaf area index and leaf area per tiller at heading and maturity stages,higher stem weight per tiller and K and Si concentrations of stem at maturity,higher dry matter weight in leaf,stem,and panicle at heading and maturity stages,and higher biomass accumulation after heading and lower biomass translocation from stem during ripening.Leaf width of upper three leaves were correlated positively,while leaf angle of upper three leaves were correlated negatively with biomass accumulation after heading,stem weight per tiller,and per unit length.Our results indicated that the grain yield advantage of JIH was ascribed mainly to the more spikelets per panicle and relatively high percentage of filled grains.Higher leaf area through leaf width and more erect leaves were associated with improved biomass accumulation and stem weighing during ripening,and were the primary morphological traits underlying higher grain yield of JIH.展开更多
文摘On the bases of archaeological discoveries, the earliest domestication of rice has been confirmed in the middle and lower Changjiang River basin, while in the region wild rice populations are found in shallow swamps under a climate with freezing winter cold. These findings lead us to-examine the past ideas about domestication and differentiation of rice. Historically, in 1930s two sub-species, indica and japonica, were proposed on the basis of sterility in F1 hybrids between them. Soon after that, the two types were classified by the associations of a number of genetically independent traits. The characteristic associations of traits have been explained by the hybrid sterility or reproductive barriers which were assumed to comprise a set of duplicate recessive lethal genes and to be an inner genetic mechanism to lead to the varietal differentiations In 1980s, the hybrid sterility between Indica and Japonica types was analyzed, and Indica, Japonica, and wide-compatibility type which gives fertile hybrids when cross to Indica and Japonica types, are proved to contain an allele, S5', S5' and S5^n, respectively at a locus on chromosome 6. And those gametes having Sj allele are found to be partially aborted in the hybrid genotypes of S5'/S5' while no gamete abortion occurs in S5'/S5^n and S5'/S5^n genotypes. Since then, the gene S5^n has been used in hybrid rice breeding to obtain fertile and vigorous hybrids between subspecies, and the long-disputed problem of hybrid sterility has been solved. Also in such studies the characteristic association of traits found in each of vadetal groups is better explained by founder effects. On the other hand, a large number of native cultivars of rice were surveyed with enzyme polymorphism in 1980s and later with molecular markers. As a result, profound genetic diversity is found in cultivated rice as well as in wild rice. These findings seem to lead us to the idea of multiple independent domestications of rice. However, before reaching such a conclusion, at least two factors, i.e., long-distance-dissemination of some genotypes and the possibilities of introgression by local wild rice to primitive cultivars need to be examined. Taking the two factors as well as the historical events into consideration, it is considered here that the perennial japonica cultivars which are close to wild rice in the Changjiang River basin were disseminated to East India through Assam or along the Bengal Bay, where they were transformed under the introgressions of local wild rice and formed a secondary center, from which some genotypes seem to be disseminated to colonies in Southeast Asia under the influence of Hinduism. Later some of the genotypes were introduced into China and constituted so called Indica type. This may be a reasonable picture for the varietal differentiation.
文摘Plant type and grain quality are two major aspects in rice breeding. Using canonical correlation analysis and canonical redundancy analysis, the relationship between plant type traits and rice grain quality traits was studied with 100 crosses derived from 10 sterile lines × 10 restorer lines. There was a complex relationship between parts of the traits of the two aspects. The angle of the 2nd leaf from the top and single panicle weight played important roles in plant type system and amylose content and grain length in grain quality system. The angle of the 2nd leaf from the top, plant height and single panicle weight had a great effect on grain quality traits, and amylose content, brown rice rate and translucency were easily influenced by plant type traits. Selection index model indicated that japonica hybrid rice in Northern China with good quality was characterized by broad flag leaf and 2nd leaf from the top, narrow and short 3rd leaf from the top, low plant height, short culm, long and more panicles and low single panicle weight.
文摘The relationship between the indica-japonica differentiation of parents and heterosis of hybrid in rice was studied with 10 sterile lines, 10 maintainer lines of the sterile lines, 38 restorer lines of Dian type hybrid rice and 40 hybrids derived from the sterile lines and restorer lines. The results indicated that there was a parabolic correlation between the indica-japonica differentiation of parents detected by RAPD markers and heterosis of hybrid rice. When certain indica-japonica differentiation of parents exists, the percentage of combinations with positve heterosis and the mean heterosis of hybrids were high. But that didn't mean too much difference of parents could lead to stronger hetesosis, that meant overdiffernt parents seldom bring positve het-erotic hybrids. Suitable difference of percent of indica alleles of parents of stronger heterotic hybrid was 12 -16%.
基金supported by the National 863 Program of China (2002AA207002)
文摘This study was designed to analyze the genetic relationship of the morphological traits of the northern japonica hybrid rice. The genetic relationship of the morphological traits of the 100 cultivars combined by the 10 sterile lines and the 10 restore lines was studied by using the principle component analysis and the SAS and QGA. The results showed that compared with the female parent, most of the morphological traits related closely to their father. The additive genetic effect and the dominant genetic effect appeared significantly, and the former was more significant than the latter. Genetic correlation analysis indicated that high plants or high plants with more leaves were in favor of improving the number and the weight of the spike and producing the hybrid vigor. The important factors influencing the plant type of the northern japonica hybrid rice were the plant height (PH), the angle of the flag leaf (FLA), flag leaf length (FLL), and the weight of the shoots and leaves (SLW), respectively. The morphological traits system of the northern japonica hybrid rice was very complex, and the restore line should be given more importance compared with the sterile line, and PH, FLA, FLL, and SLW should be considered first in the plant type breeding.
基金Supported by Science and Technology Project for Grain Production in National Science and Technology Support Program(2011BAD16B11-02YJ012012BAD04B01-02)+1 种基金Key Scientific and Technological Project of Heilongjiang Province(GA13B101)Fund for Distingnished Young Scholars of Heilongjiang Academy of Agricultural Sciences(2014)
文摘Under field conditions, three erect panicle type japonica dee cultivars and three curved panicle type japonica rice cultivars were applied as experimental materials to investigate the differences of protein composition of grains at different positions in panicles between two different panicle types of japonica rice. Accord- ing to the results, the panicle type of japonica rice had no direct correlation with albumin content, globulin content, prolamin content and glutelin content in rice, variations of protein composition of grains at different positions in panicles. There were certain correlations between soluble protein contents at different grain posi- tions in the same panicle and the flowering order of glumous flowers in the panicle. Albumin content, prolamin content and glutelin content in grains on secondary rachis branches of two panicle types (erect and curved) of japonica rice cuhivars were higher than that on primary rachis branches, while globulin content exhibited an opposite trend. Globulin content, prolamin content and glutelin content in grains at different positions demonstrated a descending order of bottom 〉 middle 〉 top, while albumin content exhibited an opposite trend. The interactions between primary and secondary rachis branches and among top, middle and bottom rachis branches significantly affected soluble protein contents.
基金supported by the National Natural Science Foundation of China(GrantNo.30871468)the Young Teacher Scientific Research Fund of Shenyang Agricultural University,China(Grant No.20081001)
文摘Two recombinant inbred line (RIL) populations, one derived from a cross between Zhongyouzao 8 (indica) and Toyonishiki (japonica) and the other from a cross between Qishanzhan (indica) and Akihikari (japonica), were grown in Liaoning and Sichuan Provinces, China, to study the panicle type index (PTI, the ratio of a number of node position on panicle axis where the secondary branch with the most numerous secondary branch grains is located to the number of primary branches) and its relationships with subspecies characteristics and yield traits. With continuous distribution of PTI and numerous of lines with different PTIs in the progenies, indica-japonica hybridization could be a method for breeding cultivars with ideal panicle type. PTI was significantly influenced by environments, with a significant decrease from Liaoning to Sichuan A significantly negative correlation was found between PTI and most of subspecies characteristics. PTI varied remarkably with different subspecies types. As a whole, it showed a trend of indica (H)〉indicalinous (H')〉japonicalinous (K')〉japonica (K) However, it is not appropriate to regard PTI as a parameter for subspecies classification in rice because it was recombined in the filial generations of cross between indica and japonica rice. The negative correlations were found between PTI and most of panicle characteristics, as well as yield-related traits. Based on PTI, most of lines from the two populations were middle dominant panicle type (the number of grains on the secondary branch is numerous in middle position of the panicle) and lower dominant panicle type (the number of grains on the secondary branch is numerous in lower position of the panicle and becomes less towards the top of panicle) with significant yield advantages.
基金financed by the Special Program of Super Rice of Ministry of Agriculture, China (02318802013231)the National Public Services Sectors (Agricultural) Research Projects, China (201303102)+1 种基金the Major Scientific and Technological Projects, Bureau of Science and Technology of Ningbo, China (2013C11001)the Innovative Training Program of Yangzhou University, China (KYLX15_1371)
文摘Late-maturity type of Yongyou japonica/indica hybrids series (LMYS) have shown great yield potential, and are being widely planted in the lower reaches of Yangtze River, China. Knowledge about suitable growing zone and evaluation of yield advantage is of practicall importance for LMYS in this region. Fifteen LMYS, two high-yielding inbred japonica check varieties (CK-J) and two high-yielding hybrid indica check varieties (CK-I) were grown at Xinghua (119.57°E, 33.05°N) of Lixiahe region, Yangzhou (119.25°E, 32.30°N)of Yanjiang region, Changshu (120.46°E, 31.41°N)of Taihu Lake region, and Ningbo (121.31°E, 29.45°N) of Ningshao Plain in 2013 and 2014. The results showed that maturity dates of the 15 were later than the secure maturity date at Xinghua and 6, 14 and 15 LMYS were mature before the secure maturity date at Yangzhou, Changshu and Ningbo, respectively. One variety was identified as high-yielding variety among LMYS (HYYS) at Yangzhou, 8 HYYS in 201:3 and 9 HYYS in 2014 at Changshu, 9 HYYS at Ningbo. HYYS here referred to the variety among LMYS that was mature before the secure maturity date and had at least 8% higher grain yield than both CK-J and CK-I at each experimental site. Grain yield of HYYS at each experimental site was about 12.0 t ha-1 or higher, and was significantly higher than CK varieties. High yield of HYYS was mainly attributed to larger sink size due to more spikelets per panicle. Plant height of HYYS was about 140 cm, and was significantly higher than check varieties. Significant positive correlations were recorded between duration from heading to maturity stage and grain yield, and also between whole growth periods and grain yield. HYYS had obvious advantage over check varieties in biomass accumulation and leaf area duration from heading to maturity stage. Comprehensive consideration about safe matudty and yield performance of LMYS at each experimental site, Taihu Lake region (representative site Changshu) and Ningshao Plain (representative site Ningbo) were thought suitable growing zones for LMYS in the lower reaches of Yangtze River. The main factors underlying high yield ofHYYS were larger sink size, higher plant height, longer duration from heading to maturity stage and whole growth periods, and higher biomass accumulation and leaf area duration during grain filling stage.
基金Grants from the National Natural Science Foundation of China, No. 39600061National Administration of Traditional Chinese Medicine, No.2003LHR13+3 种基金Medical Science Foundation of Guangdong Province, No. A2004327, A2006334the Natural Science Foundation of Guangdong, No. 04010443, 06105246 Administration Bureau of Traditional Chinese Medicine of Guangdong Province, No.1040074Technology Bureau of Guangzhou, No. 2007J1-C0041
文摘BACKGROUND: It has been shown that interleukin-1β(IL-1β) can induce fever by activating vascular endothelial cells and macrophages of the supraoptic crest to generate prostaglandin E2, which binds with receptors of the thermo-sensitive hypothalamic neurons. Lonicera japonica is one of the medicinal plants used widely in Asia for its antipyretic properties. However, these mechanisms have not yet been intensively studied. OBJECTIVE: To investigate the antipyretic effect and mechanisms of Lonicera japonica on IL-1β- induced febrile New Zealand rabbits by observing expression changes of E-type prostaglandin receptor-3 (EP3) mRNA in the preoptic anterior hypothalamus (POAH). DESIGN: A randomized controlled study. SETTING: Electrophysiological Laboratory at the Department of Pathophysiology, Medical College of Jinan University; Department of Orthopaedics, First Hospital Affiliated to Medical College of Jinan University. MATERIALS: The experiment was performed from April to December 2005, using a total of 32 New Zealand white rabbits of both sexes, weighing 1.5 2.0 kg. All the animal experiments were performed according to the internationally accepted ethical guidelines. Lonicera japonica injection was purchased from Huanghe pharmaceutical factory of Xi'an, China. IL-1βwas purchased from Sigma, USA. METHODS: A total of 32 rabbits were divided randomly into four groups: ① Normal saline (NS) control group;② Lonicerajaponica treatment group; ③ IL-1βtreatment group; and ④Lonicerajaponica plus IL-1βtreatment group. In the first 3 groups, the rabbits were given separate intravenous (i.v.) injections of l mL NS, l mL Lonicera japonica, and 100 ng IL-l β (dissolved in 0.9% NS without pyrogen). In the Lonicerajaponica plus IL-1βgroup each rabbit was given i.v. injections of l mL NS and, 30 minutes later, 100 ng IL-1 β. MAIN OUTCOME MEASURES: Colonic temperature of each rabbit was measured at 0, 10, 20, 30, 40, 50, 60, and 70 minutes after injection and the maximum temperature rise ( A T) and the temperature response index after l hour (TRII) was calculated. Subsequently, in situ hybridization (ISH) was done with an ISH kit, EP3 mRNA expression in the POAH of all groups was measured (number of positive cells and average gray scale value). RESULTS: A total of 32 rabbits were included in the result analysis, without any loss, (i) A T and TRII: there was no significant difference between the Lonicera japonica group and NS group (P 〉 0.05). The IL-1β group was significantly greater compared to NS group (P 〈 0.01). The Lonicera japonica plus IL-1β group was significantly less than the IL-1β group (P 〈 0.05). ② In the NS and Lonicera japonica groups, the EP3 mRNA expression was negative (no coloration) or only weakly positive (only a few brown yellow particles in the cytoplasm cells could not be identified). The number of positive cells and the average gray scale value were not significantly different between the two groups (P 〉 0.05). In the IL-1β group, the number of positive cells was remarkably higher and the average gray scale value was lower than the NS group (P 〈 0.0 l). In the Lonicera japonica plus IL-1β group, the number of positive cells was significantly less than the IL-1β group (P 〈 0.05). However, the average gray scale value was greater than the IL-1β group (P 〈 0.05). CONCLUSION: Lonicera japonica has obvious antipyretic effects on IL-1β-induced febrile rabbits and acts by inhibiting expression of EP3 mRNA in the POAH.
基金financed by the National Key Research and Development Program of China (2018YFD0300802)the Key Research and Development Program of Jiangsu Province, China (BE2016351)the Priority Academic Program Development of Jiangsu Higher Education Institutions, China (PAPD)
文摘The yield potential of japonica/indica hybrids(JIH)has been achieved over 13.5 t ha–1 in large-scale rice fields,and some physiological traits for yield advantage of JIH over japonica inbred rice(JI)and indica hybrid rice(IH)were also identified.To date,little attention has been paid to morphological traits for yield advantage of JIH over JI and IH.For this reason,three JIH,three JI,and three IH were field-grown at East China(Ningbo,Zhejiang Province)in 2015 and 2016.Compared with JI and IH,JIH had 14.3 and 20.8%higher grain yield,respectively,attributed to its more spikelets per panicle and relatively high percentage of filled grains.The advantage in spikelets per panicle of JIH over JI and IH was shown in number of grains on the upper,middle,and lower branches.Compared with JI and IH,JIH had higher leaf area through leaf width and lower leaf angle of upper three leaves,higher leaf area index and leaf area per tiller at heading and maturity stages,higher stem weight per tiller and K and Si concentrations of stem at maturity,higher dry matter weight in leaf,stem,and panicle at heading and maturity stages,and higher biomass accumulation after heading and lower biomass translocation from stem during ripening.Leaf width of upper three leaves were correlated positively,while leaf angle of upper three leaves were correlated negatively with biomass accumulation after heading,stem weight per tiller,and per unit length.Our results indicated that the grain yield advantage of JIH was ascribed mainly to the more spikelets per panicle and relatively high percentage of filled grains.Higher leaf area through leaf width and more erect leaves were associated with improved biomass accumulation and stem weighing during ripening,and were the primary morphological traits underlying higher grain yield of JIH.