Betaine combined with traditional Chinese medicine ointment to treat skin wounds in microbially infected diabetic mice

BACKGROUND Skin wounds are highly common in diabetic patients,and with increasing types of pathogenic bacteria and antibiotic resistance,wounds and infections in diabetic patients are difficult to treat and heal.AIM To explore the effects of betaine ointment(BO)in promoting the healing of skin wounds and reducing the inflammation and apoptosis of skin cells in microbially infected diabetic mice.METHODS By detecting the minimum inhibitory concentrations(MICs)of betaine and plant monomer components such as psoralen,we prepared BO with betaine as the main ingredient,blended it with traditional Chinese medicines such as gromwell root and psoralen,and evaluated its antibacterial effects and safety in vitro and in vivo.The skin infection wound models of ordinary mice and diabetic mice were constructed,and the OTC drugs mupirocin ointment and Zicao ointment were used as controls to evaluate the antibacterial effects in vivo and the anti-inflammatory and anti-apoptotic effects of BO.RESULTS The MICs of betaine against microorganisms such as Staphylococcus aureus(S.aureus),Candida albicans and Cryptococcus neoformans ranged from 4 to 32μg/mL.Gromwell root and psoralea,both of which contain antimicrobial components,mixed to prepare BO with MICs ranging from 16 to 64μg/mL,which is 32-256 times lower than those of Zicao ointment,although the MIC is greater than that of betaine.After 15 days of treatment with BO for USA300-infected ordinary mice,the wound scab removal rates were 83.3%,while those of mupirocin ointment and Zicao ointment were 66.7%and 0%,respectively,and the differences were statistically significant.In diabetic mice,the wound scab removal rate of BO and mupirolacin ointment was 80.0%,but BO reduced wound inflammation and the apoptosis of skin cells and facilitated wound healing.CONCLUSION The ointment prepared by mixing betaine and traditional Chinese medicine can effectively inhibit common skin microorganisms and has a strong effect on the skin wounds of sensitive or drug-resistant S.aureus-infected ordinary mice and diabetic mice.

Calmoseptine Ointment Tube皮肤保护剂联合院内制剂妇洁康对失禁性皮炎大鼠的作用及经济效益分析

目的:研究和探讨Calmoseptine Ointment Tube皮肤保护剂联合院内制剂妇洁康对失禁性皮炎大鼠模型的作用,并分析其经济效益,以期选出价格较实惠且效果较好的失禁性皮炎的防治方案。方法:建立失禁性皮炎大鼠模型,成功后将32只大鼠分为四组,即妇洁康洗剂组(A组)、3M液体敷料联合造口护肤粉组(B组)、Calmoseptine Ointment Tube皮肤保护剂组(C组)、妇洁康联合Calmoseptine Ointment Tube皮肤保护剂组(D组),每组8只。根据皮肤的症状评估大鼠的失禁性皮炎严重程度,通过追踪皮炎边缘测量皮炎面积,同时进行组织病理学评估其组织损伤,免疫生化分析其嗜中性粒细胞、巨噬细胞、淋巴细胞以及血管内皮生长因子、增殖细胞核抗原等水平。并结合成本效果分析方法分析其经济效益。结果:治疗后,A、B、C三组大鼠的失禁性皮炎的严重程度、面积和恢复时间比较无显著差异(P>0.05);与D组相比,A、B、C三组大鼠失禁性皮炎的严重程度显著更高,面积更大,恢复时间更长(P<0.05)。组织病理学分析显示,A、B、C三组大鼠表皮萎缩、毛囊萎缩、胶原蛋白流失、水肿等各组织情况评分比较无显著差异(P>0.05),但与D组相比,A、B、C三组大鼠的各组织指标评分显著更高(P<0.05)。免疫生化分析显示,A、B、C、D四组大鼠的PCNA水平比较无显著差异(P>0.05);A、B、C三组大鼠的嗜中性粒细胞、巨噬细胞、淋巴细胞、VEGF水平比较无显著差异(P>0.05);与D组相比,A、B、C三组大鼠的嗜中性粒细胞、巨噬细胞、淋巴细胞、VEGF水平显著更高(P<0.05)。根据不同经济效益分析可知,CEA分析显示,D组>C组>B组>A组;CMA分析显示,D组>C组>B组>A组;C/E分析显示,D组>C组>A组>B组。ΔC/ΔE分析显示,成本额最高为D组,与其他三组进行比较,D组与A组ΔC/ΔE为7.94;D组与B组ΔC/ΔE为11.89;D组与C组ΔC/ΔE为7.57。可见在经济成本上,D组明显要高于其他三组,但从增益量上看,D组虽然治疗成本较高,但所产生的效果也较高,而且,增加的治疗成本可以带来更加满意的增量效果。结论:Calmoseptine Ointment Tube皮肤保护剂联合院内制剂妇洁康对失禁性皮炎具有较好的治疗效果,能够缩小皮炎面积,缩短恢复时间,并改善皮肤组织情况,提高免疫力。同时该治疗方案虽然治疗成本较高,但治疗效果也更好,更具经济性,能够节省治疗的整体费用,值得临床推广应用。

Skin Toxicity of Wufang Babu Ointment to Zebrafish

[Objectives]Using wild-type AB strain of zebrafish as experimental animal,this study investigated the damaging effect of Wufang Babu Ointment on skin cells,in order to evaluate the skin toxicity of Wufang Babu Ointment.[Methods]Wild-type AB strain of zebrafish with an age of 2 d were taken and fed in different concentrations of Wufang Babu Ointment solution for 24 h.The number of deaths in each group of zebrafish was recorded,and the mortality rate was calculated.Using Origin 8.0 software,the maximum non lethal concentration(MNLC)was simulated.Zebrafish raised in different concentrations of Wufang Babu Ointment solution for 24 h were placed under an anatomical microscope for taking photos,to analyze and calculate the incidence of skin damage in zebrafish.Based on the statistical analysis results of this indicator,the skin toxicity of Wufang Babu Ointment was evaluated.[Results]The MNLC of Wufang Babu Ointment on zebrafish was 671μg/mL;Wufang Babu Ointment can induce skin damage at the concentrations of 224μg/mL(1/3 MNLC)and 671μg/mL(MNLC).[Conclusions]Wufang Babu Ointment had certain skin toxicity to zebrafish.

Preparation Process and Quality Standard of Rougan Huaxian Ointment

[Objectives] To establish the preparation process and quality standard of Rougan Huaxian ointment.[Methods] The L_(9)(3^(4)) orthogonal test was employed to optimize the preparation process by considering the multiplication of water addition, extraction time, and extraction frequency as influencing factors. The dry paste yield was utilized as the evaluation criterion, in conjunction with the actual production conditions. Thin layer chromatography was employed to identify Radix Astragali, Lycii Fructus, Herba Dendrobii, and Rhizoma Polygoni Cuspidati. Ethanol served as the solvent for the determination of ethanol-soluble extractives using the cold immersion method.[Results] The preparation process was conducted as follows: the specified quantity of medicinal materials was combined with water for extraction purposes, performed in two separate stages. In each stage, eight times the amount of water was added. The first extraction lasted for 1.5 h, while the second extraction was completed in 1.0 h. The resulting liquid was then concentrated into a thick paste with a relative density ranging from 1.25 to 1.30 at a temperature of 60 ℃. The thin-layer chromatography analysis of Radix Astragali, Lycii Fructus, Herba Dendrobii, and Rhizoma Polygoni Cuspidati demonstrated distinct spots, effective separation, and the absence of interference from negative samples. Additionally, the ethanol-soluble extractives yielded a minimum of 8.0% in terms of dry weight.[Conclusions] The preparation process for Rougan Huaxian ointment is both stable and feasible. Furthermore, the quality standards established for this preparation are unique and reproducible, thereby facilitating effective quality control.

Clinical Efficacy of Huanglian Ointment in the Treatment of Chronic Eczema

Objective:To investigate and analyze the clinical efficacy of Huanglian ointment in the treatment of chronic eczema.Methods:114 cases of chronic eczema patients admitted to the Department of Dermatology of our hospital from January 2023 to January 2024 were selected as the study subjects.They were divided into a study group(n=57)and a reference group(n=57)using the double-blind method.The study group was treated with dialyzed Huanglian ointment,while the reference group was treated with conventional treatment.Symptom scores,inflammatory levels,treatment effects,and immune indexes of the two groups were similar before treatment.Results:After treatment,the symptom scores of the study group were significantly lower than those in the reference group(P<0.05).Besides,the interleukin-2(IL-2),tumor necrosis factor-a(TNF-a),interferon-(IFN-),and other inflammatory indicators of the study group were significantly better than those of the reference group(P<0.05).The total efficacy of treatment in the study group was significantly higher than that in the reference group(P<0.05).Furthermore,after treatment,the immunity indexes such as CD3+,CD4+,and CD8+of the study group were significantly better than those of the reference group,(P<0.05).Conclusion:Huanglian ointment is extremely effective for treating chronic eczema so this treatment program should be popularized.

复肾膏联合真武汤治疗脾肾阳虚型肾性水肿的疗效及对肾小管损害标志物、凝血指标的影响

目的 观察复肾膏联合真武汤治疗肾性水肿脾肾阳虚型的疗效及对肾小管损害标志物、凝血指标的影响。方法 选取80例肾性水肿脾肾阳虚型患者为研究对象,按照随机数字表法分为对照组和治疗组,对照组40例予真武汤治疗,治疗组40例复肾膏联合真武汤治疗。2组均10天为1个疗程,连续3个疗程后统计疗效。比较2组治疗前及治疗1、2、3个疗程后肾小管损害标志物[中性粒细胞明胶酶相关脂质运载蛋白(NGAL)、肾损伤分子-1(KIM-1)]、肾功能[24 h尿蛋白定量(24 h Upro)、尿素氮(BUN)、血肌酐(SCr)、血清白蛋白(ALB)]、凝血功能[D-二聚体(D-D)、活化部分凝血活酶时间(APTT)、血小板计数(PLT)]变化,观察记录2组治疗过程中不良反应情况。结果 治疗组总有效率92.50%(37/40),明显高于对照组的70.00%(28/40)(P<0.05)。治疗1、2、3个疗程后,2组NGAL、KIM-1、24 h Upro、BUN、SCr水平均较本组治疗前明显降低(P<0.05),ALB均较本组治疗前明显升高(P<0.05),且2组组间同期比较差异均有统计学意义(P<0.05)。治疗1、2、3个疗程后,2组D-D、APTT均较本组治疗前明显降低(P<0.05),且治疗组均低于对照组同期(P<0.05);治疗3个疗程后,2组PLT均较本组治疗前明显降低(P<0.05),且治疗组低于对照组同期(P<0.05)。治疗组不良反应发生率为7.50%(3/40),对照组为12.50%(5/40),2组不良反应发生率比较差异无统计学意义(P>0.05)。结论 复肾膏联合真武汤治疗肾性水肿脾肾阳虚型可有效改善肾功能及凝血功能,降低NGAL、KIM-1水平,效果显著。

给氧负压封闭引流联合湿润烧伤膏序贯治疗高海拔地区下肢慢性溃疡疗效分析

目的探讨给氧负压封闭引流联合湿润烧伤膏序贯治疗高海拔地区下肢慢性溃疡的临床疗效。方法选取2019年1月至2023年3月若尔盖县人民医院收治的60例下肢慢性溃疡患者作为研究对象,按照创面不同治疗方法将其分为观察组(30例)和对照组(30例),观察组患者予以给氧负压封闭引流联合湿润烧伤膏序贯治疗,对照组患者单纯予以湿润烧伤膏治疗,对比两组患者创面肉芽组织状态、病原菌感染情况、愈合率以及住院时间。结果治疗3周后,观察组患者创面肉芽组织评分明显高于对照组、病原菌阳性率低于对照组(Z/χ^(2)=-4.332、4.800,P<0.001、P=0.028);观察组患者治疗3周后创面愈合率明显高于对照组、住院时间明显短于对照组(t=6.517、7.211,P均<0.001)。结论给氧负压封闭引流联合湿润烧伤膏序贯治疗高海拔地区下肢慢性溃疡创面,能够有效促进肉芽组织生长,抑制病原菌增殖,加快创面愈合,缩短住院时间。

固本防喘膏在儿童哮喘缓解期的应用效果

目的探讨固本防喘膏在儿童哮喘缓解期的应用效果。方法选取2022年3月至2024年3月于常州市中医医院儿科就诊的90例哮喘缓解期患儿作为研究对象,采用随机数字表法分为对照组和试验组,每组45例。对照组给予玉屏风颗粒治疗,试验组采用固本防喘膏治疗,比较两组患儿临床疗效、肺功能及免疫功能。结果治疗后,试验组总体控制率优于对照组(91.11%vs 75.56%)(P<0.05);试验组第1秒用力呼气容积、用力肺活量、呼气流量峰值均高于对照组(P<0.05);试验组血清免疫球蛋白(Ig)A、IgG、IgM、CD4^(+)和CD4^(+)/CD8^(+)水平均高于对照组,CD8^(+)水平低于对照组(P<0.05)。结论固本防喘膏在儿童哮喘缓解期具有较好的应用效果,可提高总体控制率,改善肺功能及免疫功能。

五物甘草生摩膏穴位推拿防治肺脾气虚证脑瘫患儿反复呼吸道感染临床研究

目的:观察五物甘草生摩膏穴位推拿防治肺脾气虚证脑瘫患儿反复呼吸道感染(RRTI)的临床疗效。方法:将60例2022年3月—2023年4月于河南中医药大学第一附属医院就诊的肺脾气虚证RRTI脑瘫患儿,采用随机数字表法分为观察组与对照组各30例。观察组以五物甘草生摩膏为介质进行穴位推拿治疗,对照组给予口服脾氨肽治疗。疗程8周,观察2组临床疗效、中医证候评分;随访12个月,记录2组呼吸道感染减少次数及不良反应。结果:观察组总有效率为93.33%(27/30),对照组为63.33%(19/30),2组比较,差异有统计学意义(P<0.05)。治疗后,2组恶风、自汗、气短、面色苍白、少气懒言、不思饮食、形体消瘦、大便溏薄等中医证候评分均较治疗前降低(P<0.05),且观察组各项评分均低于对照组(P<0.05)。随访12个月,观察组呼吸道感染减少次数明显多于对照组(P<0.05);其中观察组上呼吸道感染减少次数多于对照组(P<0.05),2组下呼吸道感染(包括气管炎/支气管炎感染、肺炎感染)减少次数比较,差异无统计学意义(P>0.05)。治疗期间,2组患儿均未见明显不良反应。结论:五物甘草生摩膏穴位推拿治疗能改善肺脾气虚证脑瘫患儿RRTI的临床症状,减少上呼吸道感染次数,且远期疗效较好。

Methodology Validation of Microbial Limit Test of Bupi Qiangli Ointment

[Objective] This study was conducted to establish a microbial limit test method for Bupi Qiangli Ointment. [Method] The conventional method and medium dilution method were used for bacterial, mold and yeast counting in sample recovery test. [Result] The medium dilution method （1：10 test solution, 0.5 ml/plate） could effectively eliminate the inhibition effect of the Bupi Qiangli Ointment, and the recovery of Staphylococcus aureus was greater than 70% in the 3 batches of samples; and the conventional method exhibited the recoveries of E. coil, Bacillus subtilis, Candida albicans and Aspergillus greater than 70% in the 3 batches of samples. [Conclusion] Due to Bupi Qiangli Ointment has strongly antibacterial effect on Staphylococcus au- reus, the medium dilution method was used for bacterial counting, and the conventional method was used for mold and yeast counting; and the conventional method was used for controlled bacterium examination of E. coll.

基于多指标权重综合评分和正交试验的调经养巢膏提取工艺优选

目的优选调经养巢膏的提取工艺。方法以加水倍数、提取时间、提取次数为考察因素,以阿魏酸、金丝桃苷、松果菊苷、丹酚酸B、淫羊藿苷的溶出量和出膏率为评价指标,分别采用层次分析(AHP)法、CRITIC法及AHP-CRITIC混合加权法确定各指标的权重系数,采用L_(9)(3^(4))正交试验优选提取工艺。结果优选工艺为复方中药加水煎煮3次,第1次加7倍量水室温浸泡40 min后,煎煮60 min,第2,3次各加5倍量水,煎煮50 min。3次验证试验测得浓缩液中阿魏酸、金丝桃苷、松果菊苷、丹酚酸B、淫羊藿苷的平均溶出量分别为0.0691,0.1307,0.8739,0.8645,0.2175 mg/mL,平均出膏率为40.35%,综合评分为93.35。结论优选工艺科学可行、稳定可控,可为后期调经养巢膏的开发利用提供参考。

点阵CO_(2)激光联合他克莫司软膏治疗稳定期白癜风效果分析

目的:探讨点阵CO_(2)激光联合他克莫司软膏治疗稳定期白癜风对患者白斑面积及美学满意度的影响。方法:选取2022年2月-2023年1月宝鸡市中心医院收治的78例稳定期白癜风患者作为研究对象,采用随机数字表法分为A组(26例)、B组(26例)和C组(26例)。A组给予点阵CO_(2)激光联合他克莫司软膏治疗,B组给予他克莫司软膏,C组给予点阵CO_(2)激光治疗。三组均持续治疗16周并随访3个月。比较三组治疗16周后的临床疗效、复色率、白斑面积及美学满意度,治疗前、治疗16周后的肉眼下皮损情况、免疫功能及炎症反应,随访期间的安全性,预后。结果:治疗16周后,A组、B组、C组总有效率分别为96.15%、69.23%、73.08%,A组高于B、C组(P<0.05)。与治疗前比较,治疗16周后,三组皮损色素岛形成、毛囊周围毛细血管扩张、皮损周围色素的患者占比均升高,A组高于B、C组(P<0.05)。治疗16周后,A组复色率均高于B、C组;A组白斑面积均低于B、C组;A组、B组、C组美学满意率分别为96.15%、65.38%、69.23%,A组高于B、C组(P<0.05)。与治疗前比较,治疗16周后,三组血清CD4^(+)、CD36(+)CD8^(+)、CD4^(+)CD45^(+)水平均升高,且A组高于B、C组,C组高于B组;三组血清CD8+、白细胞介素-17(IL-17)、白细胞介素-22(IL-22)水平均降低,且A组低于B、C组,C组低于B组(P<0.05)。随访期间,三组不良反应发生率比较,差异无统计学意义(P>0.05)。随访3个月后,A、B、C组复发率分别为3.85%、30.77%、15.38%,A组低于B、C组(P<0.05)。结论:在稳定期白癜风的治疗中,点阵CO_(2)激光联合他克莫司软膏治疗可显著提高患者免疫功能,减轻患者炎症反应,并缓解患者皮损症状,减少白斑面积,提高复色率,进而提高患者美学满意度,且安全性良好,复发率较低。

磺胺嘧啶银乳膏联合温阳生肌膏与磺胺嘧啶银乳膏外敷治疗糖尿病足溃疡效果的随机对照分析

目的比较磺胺嘧啶银乳膏联合温阳生肌膏与磺胺嘧啶银乳膏外敷治疗糖尿病足溃疡(DFU)的效果。方法采用随机数字表法将60例DFU患者平均分为2组。在给予抗感染、溃疡消毒清洁、原发病控制、抗血小板聚集等基础上,联合组采用磺胺嘧啶银乳膏联合温阳生肌膏外敷,对照组单纯应用磺胺嘧啶银乳膏外敷,4个疗程后比较2组治疗前后的溃疡皮损面积和创面色泽评分,评价临床疗效。结果治疗前2组溃疡皮损面积和创面色泽评分的差异均无统计学意义(P>0.05);治疗28 d后,2组溃疡皮损面积和创面色泽评分均较治疗前改善,其中联合组的改善效果均优于对照组,差异均有统计学意义(P<0.05)。联合组的总有效率高于对照组,差异有统计学意义(P<0.05)。治疗过程中2组均未出现明显不良反应。结论与单纯外敷磺胺嘧啶银乳膏比较,联合温阳生肌膏外敷治疗DFU,更利于改善溃疡皮损面积和创面色泽评分,效果更好且无明显不良反应。

积雪苷霜软膏联合调Q1064nm激光治疗黄褐斑效果分析

目的:探究积雪苷霜软膏联合调Q1064 nm激光治疗黄褐斑患者的疗效。方法:选取2021年1月-2022年1月笔者医院皮肤科收治的黄褐斑患者为研究对象,采用简单抽样法随机分为软膏组、激光组、联合组,各42例,其中软膏组采用积雪苷霜软膏单独治疗,激光组采用调Q1064 nm激光单独治疗,联合组采用调Q1064 nm激光联合积雪苷霜软膏治疗,比较三组患者不良反应发生情况,并对比三组患者治疗前及治疗6个月后的疗效[黄褐斑面积严重指数(MASI)、黑素指数、黄褐斑颜色、毛细血管扩张评分]、皮肤菌群(微球菌、需氧革兰阴性杆菌、痤疮丙酸杆菌)水平、疾病相关激素水平[血清卵泡刺激素(FSH)、雌二醇(E2)、促黑激素(MSH)、超氧化物歧化酶(SOD)、过氧化脂质(LPO)]、皮肤生理参数[经皮水分丢失(TEWL)、表皮油脂含量、角质层含水量]水平变化。结果:联合组治疗6个月后疗效相关指标、皮肤菌群(微球菌、痤疮丙酸杆菌)、疾病相关激素水平(FSH、E2、MSH、LPO)均显著低于软膏组及激光组(P<0.05);SOD水平显著高于软膏组及激光组(P<0.05)。治疗6个月后,三组患者需氧革兰阴性杆菌数量、皮肤生理参数均差异无统计学意义(P>0.05)。治疗后1年进行随访时发现,联合组复发率为33.22%(13/39)低于软膏组复发率42.11%(16/38)、激光组复发率为47.37%(18/38),但三组差异均无统计学意义(P>0.05)。结论:积雪苷霜软膏联合调Q1064 nm激光对黄褐斑患者皮肤具有良好疗效,能够明显改善机体相关激素水平,调节皮肤微环境,维持皮肤屏障正常功能,具有较高安全性。

湿润烧伤膏治疗寻常型银屑病临床研究

目的研究湿润烧伤膏治疗寻常型银屑病的临床疗效。方法选取2021年7月至2023年4月濮阳市油田总医院收治的68例中重度寻常型银屑病患者作为研究对象,按照不同治疗方法将其分为观察组(34例)与对照组(34例)。对照组患者采用异维A酸软胶囊口服治疗,观察组患者在异维A酸软胶囊口服基础上加用湿润烧伤膏治疗,对比观察两组患者血清血管内皮生长因子(VEGF)与肿瘤坏死因子-α(TNF-α)水平、皮损严重程度、生活质量及临床疗效。结果治疗8周后,观察组患者血清VEGF、TNF-α水平以及银屑病皮损面积和严重性指数(PASI)与皮肤病生活质量指数(DLQI)评分均明显低于对照组(t=5.047、3.921、3.539、4.236,P均<0.001)。观察组患者临床总有效率为94.12%,明显高于对照组患者的临床总有效率76.47%(χ^(2)=4.221,P=0.040)。结论湿润烧伤膏能够通过降低局部增生及炎症水平改善寻常型银屑病患者临床症状,疗效显著。

全蝎通络膏对小儿目劄肝经风热型疗效观察

目的:探讨全蝎通络膏对小儿目劄肝经风热型疗效观察。方法:选取2019年1月至2023年12月佳木斯市中医医院眼科确诊为小儿目劄患儿66例,随机分为对照组及通络组,对照组予以西医常规治疗,通络组在对照组基础上加用全蝎通络膏治疗,观察两组治疗效果、瞬目次数、证候积分、泪膜破裂时间等。结果:经治疗,通络组有效率为84.38%,对照组有效率为66.67%,两组对比有统计学意义(χ^(2)=4.340,P<0.05)。两组治疗前瞬目次数、证候积分及BUT对比,无统计学差异(P>0.05);治疗后通络组瞬目次数、证候积分、BUT均有明显改善,相比治疗前存在统计学差异(P<0.05),且通络组治疗后与对照组比较,存在统计学差异(t=3.256、4.569、2.379,P<0.05);对照组治疗前后仅瞬目次数存在统计学差异(P<0.05)。结论:全蝎通络膏对小儿目劄肝经风热型疗效观察疗效较好。

饵激光、308 nm LED紫外线联合卤米松乳膏治疗白癜风临床效果

目的:观察饵激光、308 nm LED紫外线联合卤米松乳膏治疗白癜风患者的临床效果。方法:招募2022年1月—2023年1月东莞市大朗医院及东莞市滨海湾中心医院皮肤科60例稳定期白癜风患者。按随机数字表法将患者分为对照组和试验组,每组30例。对照组采用308 nm LED紫外线联合外涂卤米松乳膏治疗,试验组采用饵激光、308 nm LED紫外线联合外涂卤米松乳膏治疗。比较两组白癜风面积评分指数(vitiligo area scoring index,VASI)、WOOD灯评分及治疗效果。结果:治疗前,两组VASI比较,差异无统计学意义(P>0.05),开始治疗第3个月,试验组VASI较对照组降低,但比较差异无统计学意义(P>0.05),开始治疗第6个月和第12个月,试验组VASI均较对照组降低(P<0.05)。开始治疗第3、6、12个月,试验组WOOD灯评分均较对照组升高(P<0.05)。试验组总有效率比对照组高(P<0.05)。结论:饵激光、308 nm LED紫外线联合卤米松乳膏治疗白癜风效果更好,色素恢复更为明显。

烧伤再生医疗技术治愈双足背深Ⅱ度烧伤1例报告

给予2020年2月南充市中医医院收治的1例双足背烧伤患者在敏感抗生素抗感染、营养支持、调控血糖、抗休克等对症支持治疗的基础上加用黄连解毒汤口服,并给予局部创面于坏死组织清除、耕耘减张处理后采用烧伤再生医疗技术治疗,治疗第22天,双足背创面基本愈合,患者出院;出院7 d后随访,创面完全愈合,愈后皮肤无明显瘢痕增生,运动功能正常。

Study on effect of Traditional Chinese Medicine Jianpi Chushi decoction and ointment on chronic eczema

Objective:To study the effects of Traditional Chinese Medicine Jianpi Chushi decoction and ointment on chronic eczema.Methods:DNCB acetone solution was used to sensitize the skin of back and ears of 36 rats in order to establish chronic eczema model.A total of 36 rats were divided into four groups of 9 randomly including oral medicine group,external inunctum group,combination therapy group,and model control group respectively.Besides,the blank group of 4 healthy rats were set.The oral medicine group was given Traditional Chinese Medicine Jianpi Chushi decoction [(Poria cocos,Chinese yam,Cortex dictamni,Zaocys dhumnade,Rhizoma atractylodis,Pericarpium citri reticulatae,Scutellaria baicalensis,Radix Sophorae Flavescentis,Raw Radix Paeoniae Alba,Licorice roots(Northwest Origin)] by gastric infusion(1.6 g/m L·5 m L/d); the external inunctum group was given Qingpeng ointment on the skin,the combination therapy group was given Jianpi Chushi decoction by gastric infusion and Qingpeng ointment combination therapy.The model control group was given normal saline(NS) of the same volume by gastric infusion and vaseline on skin.Continuous administration 15 d and stopped for 3 d.The thickness difference and weight difference of left and right ear of every group were measured and the degree of ear swelling were evaluated.The CD4^+ and CD8^+ content and the IL-2,IL-4 level of serum were detected,and the inflammatory cells counts of back skin were recorded.Results:After treatment,the degree of ear swelling of oral medicine group,external inunctum group and combination therapy group significant decreased compared with model control group(P<0.05).The CD4^+,CD8^+ cell content and IL-2 level of oral medicine group,external inunctum group,combination therapy group and model control group significant decreased compared with blank group,and IL-2 level and the inflammatory cells count increased.After 15 d of treatment,the CD4^+,CD8^+ cell content and IL-2 level of serum of oral medicine group,external inunctum group and combination therapy group raised and the IL-4 level and the inflammatory cells count had significant decreased compared with model control group,and the effect of combination therapy group was more obvious(P<0.05).Conclusions:Qral Jianpi Chushi decoction could treat chronic eczema effectively,and oral Chinese medicine combined with ointment could enhance and speed up the efficacy.

湿润烧伤膏联合纳米银敷料治疗重度烧伤患者的效果

目的:观察湿润烧伤膏联合纳米银敷料治疗重度烧伤患者的效果。方法:选取2020年9月至2023年5月于该院行清创植皮术的78例重度烧伤患者进行前瞻性研究,按照随机数字表法将其分为对照组和观察组各39例。两组均给予清创植皮术,在此基础上,对照组给予纳米银敷料治疗,观察组在对照组基础上联合湿润烧伤膏治疗。比较两组临床疗效,预后指标(14 d创面细菌培养阳性率、创面感染率、色素沉着率)水平,瘢痕严重程度[温哥华瘢痕量表(VSS)]评分,创面愈合时间,治疗前后炎性因子[白细胞介素-2(IL-2)、白细胞介素-6(IL-6)、肿瘤坏死因子-α(TNF-α)]水平,以及并发症发生率。结果:观察组治疗总有效率为94.87%(37/39),高于对照组的74.36%(29/39),差异有统计学意义(P<0.05);观察组14 d创面细菌培养阳性率、创面感染率、色素沉着率均低于对照组,差异有统计学意义(P<0.05);观察组VSS评分低于对照组,创面愈合时间短于对照组,差异均有统计学意义(P<0.05);治疗后,两组IL-2、IL-6、TNF-α水平均低于治疗前,且观察组低于对照组,差异有统计学意义(P<0.05);观察组并发症发生率为2.56%(1/39),低于对照组的20.51%(8/39),差异有统计学意义(P<0.05)。结论:湿润烧伤膏联合纳米银敷料治疗重度烧伤患者可提高临床疗效,改善预后,减轻瘢痕和机体炎症反应,降低并发症发生率,效果优于单纯纳米银敷料治疗。