Fufang E’jiao Jiang’s effect on immunity,hematopoiesis,and angiogenesis via a systematic“compound-effect-target”analysis

Fufang E’jiao Jiang(FEJ)as a healthy food consisting of medicine food homology materials approved by China’s Ministry of Health has been extensively applied to replenish qi and nourish blood,and it has a positive impact on women’s health.To find out the material basis and mechanism of FEJ,a systematic“compoundeffect-target”analysis including chemical composition resolution,zebrafish,network pharmacology,molecular docking,transcriptome,and bibliometric analysis was adopted.124 chemical components including ginsenosides,and phenylethanoid glycosides in FEJ were discovered,and effects of FEJ on promoting the generation of immune cells,erythropoiesis and angiogenesis in zebrafish were exhibited.Based on network pharmacology,molecular docking and in vivo activity assay,6 compounds including jionoside A1,isoacteoside,echinacoside,acteoside,lobetyolin,and rehmannioside D were identified as active components of FEJ.Transcriptome data showed that several pathways such as complement and coagulation cascades,ECM-receptor interaction,and PI3K-Akt signaling pathway were associated with proangiogenic effect of FEJ.19 common targets were obtained through combined analysis of network pharmacology and transcriptomics,and 5 targets of them were verified by PCR.The bibliometric analysis of these common targets revealed that FEJ was related to energy metabolism,pathway in cancer,etc.,which was consistent with the results of network pharmacology and transcriptome.The studies suggested that FEJ could replenish qi and nourish blood through multi-compound and multi-targets.

Jiao-tai-wan Up-regulates Hypothalamic and Peripheral Circadian Clock Gene Cryptochrome and Activates PI3K/AKT Signaling in Partially Sleep-deprived Rats

This study aims to explore the effect and mechanism of Jiao-tai-wan （JTW） on systemic and tissue-specific inflammation and insulin resistance in obesity-resistant （OR） rats with chronic partial sleep deprivation （PSD）. OR rats with PSD were orally given JTW and Estazolam for 4 weeks. The amount of food intake and metabolic parameters such as body weight increase rate, fasting plasma glucose （FPG）, fasting insulin （FINS）, homeostasis model assessment-insulin resistance （HOMA-IR） and plasma inflammatory markers were measured. The expression levels of circadian proteins cryptochrome 1 （Cryl） and cryptochrome 2 （Cry2） in hypothalamus, adipose and liver tissues were also determined. Meanwhile, the mRNA expression of inflammatory markers, activity of nuclear factor kappa B （NF-κB） p65 protein, as well as the expression levels of insulin signaling pathway proteins in hypothalamus, adipose and liver tissues were measured. Additionally, cyclic adenosine 3＇, 5＇-monophosphate （cAMP） and activity of vasodilator-stimulated phosphoprotein （VASP） in hypothalamus tissue were measured. JTW significantly decreased the body weight increase rate and food intake, ameliorated systemic inflammation and insulin resistance. JTW effectively ameliorated inflammation and increased PI3K/AKT signaling activation in hypothalamus, adipose and liver. Interestingly, all these changes were associated with the up-regulation of circadian gene Cryl and Cry2 protein expression. We also found that in hypothalamus tissue of PSD rats, down-regulation of Cryl and Cry2 activated cAMP/PKA signaling and then led to inflammation, while JTW inhibited this signaling. These results suggested that JTW has the beneficial effect on ameliorating inflammation and insulin resistance in partially sleep-deprived rats by up-regulating Cry expression.

Acupuncture at the San Jiao meridian affects brain stem tissue G protein content in a rat migraine model

BACKGROUND： G protein is closely associated with vasomotion. Vasomotor dysfunction accompanies migraine attack. OBJECTIVE： To investigate the effects of the San Jiao meridian acupuncture on G protein content in a rat migraine model. DESIGN, TIME AND SETTING： The present randomized grouping, cellular and molecular biological level trial was performed at the Institute of Integrated Traditional Chinese and Western Medicine, Xiangya Hospital, Central South University ＆ Key Laboratory for Tumor Proteomics of Ministry of Health between October 2003 and June 2004. MATERIALS： Forty healthy, male, Sprague Dawtey rats were included in this study. The G6805-2A electro-acupuncture apparatus was a product of Shanghai Huayi Medical Instrument Factory, China. Nitroglycerin was produced by Guangzhou Mingxing Pharmaceutical Factory, China. Antibodies against inhibitory and stimulatory G proteins were purchased from Sigma Chemical Company, USA. METHODS： All 40 rats were randomly and evenly divided into 4 groups. In the blank control group, the rats remained untouched. Rats from the normal control group were subcutaneously administered 2 mL/kg physiological saline. In the model group, migraine was induced with a subcutaneous injection of 10 mg/kg nitroglycerin （5 g/L）, and the rats received no further treatment. In the acupuncture-treated group, 30 minutes after migraine induction, acupuncture was performed at the bilateral Waiguan （SJ 5） and Yifeng （SJ 17） points, with an acupuncture depth of 1 mm. Electric-stimulation parameters of 20 Hz for low frequency, 40 Hz for high frequency, and 0.5-1.0 mA for current intensity were set. Ten acupuncture sessions were applied, with 20-minute low-frequency and 20-minute high-frequency stimulation and 3 seconds of interval time. MAIN OUTCOME MEASURES： Inhibitory and stimulatory G protein contents were detected by Western blot analysis. RESULTS： At 4 hours after migraine induction, compared with the blank control and normal control groups stimulatory G protein concentration was significantly increased, while inhibitory G protein levels were significantly decreased in the model group （P 〈 0.01 ）. In the acupuncture-treated group, both stimulatory and inhibitory G protein concentrations were significantly increased following acupuncture treatment （P 〈 0.01）, but stimulatory G protein levels were less and the inhibitory G protein concentrations were greater compared to the model group （P 〈 0.01 ）. There was no significant difference in stimulatory and inhibitory G protein levels between the blank control and normal control groups （P 〉 0.05）. CONCLUSION： Dysfunctional G protein signal transductions in the rat brain stem may be responsible tor migraine attack. Acupuncture at the San Jiao meridian ameliorates migraines by mediating the G protein signal transduction pathway.

Rong Shuan Jiao Nang in the treatment of acute mountain sickness and high altitude myocardial ischemic syndrome in Yushu

To evaluate the therapeutic effects of Rong Shuan Jiao Nang （RSJN） on treatment of acute mountain sickness （AMS） and high altitude myocardial ischemic syndrome in workers in Yushu, three groups were studied： group A （60 patients with AMS, given RSJN）, group B （15 patients with altitude myocardial ischemic syndrome, given RSJN）, and group C （control, without drugs）. All studied subjects were lowland workers who were first time entry to Yushu for work at an altitude of 4 250 m. During the course of treatment, a routing physical examina- tion was performed, AMS Lake Louise Scores were estimated, arterial oxygen saturation （SaO2）, electrocardiography and hemoglobin concentration were measured before and after using RSJN for 10 days. In group A, the effective rate was 68 %, symptomatic improvement in 54 cases （90 %） within 5 days. In group B, the effective rate was 93 %, episodes of angina pectoris stopped in 12 patients within 3 - 7 days, one lasted 8 days. After treatment, the level of SaO2 increased 15.5 %, 21.8 % and 5.6 % in group A, group B and group C, respectively. RSJN tak- en at the start of the arrival at Yushu can decrease AMS scores and facilitate cure. If taken after the illness has begun, RSJN may help lessen symptoms, especially effectively improved angina pectoris of the high altitude myocardial ischemic syndrome. Symptoms usually subside after 3 - 8 days. RSJN should be continually used lbr at least 7 days after ascent.

Structure and Distribution of the San Jiao and Cou Li-Recognized Interstitium in Human Tissues

Since the Yellow Emperor’s Inner Canon(c.200 bce),Traditional Chinese Medicine(TCM)has held that the san jiao system is the largest anatomical structure in the human body,and that it consists of a network comprising the large cavities in the body trunk and the small interstitial spaces between the tissues and cells throughout the body.More than 2000 years later,and according to recent scientific reports in America,this network of structures has been recognized by modern medicine.The two theories,TCM’s san jiao and its system of spaces(腠còu),and the recent scientific discovery of an interstitial network in the human body,are quite similar in structure,distribution and function.

The early Proterozoic metamorphic geodynamics of the Jiao-Liao Massif, east China

Considering the mineral evolution and tectonic deformational history of the early Proterozoic metamorphic sequences in the Jiao Liao Massif, very similar CCW PTt D paths were established in east Liaoning and east Shandong district within this massif. Their style displays the process of developing and closing of some rifted depression belts within the rather stable continental crust of the Jiao Liao Massif during early Proterozoic, and they may be treated as a common kind of metamorphic geodynamic model.

A Brief Discussion on the Concept of Location San Jiao

San Jiao is one of the most controversial concepts in traditional Chinese medicine.With the changes of history,the concept of San Jiao was developed into another concept,"Location San Jiao",on the basis of"Liu Fu San Jiao".However,the appearance of"Location San Jiao"makes the concept of San Jiao more complicated and controversial.This paper will explore from the origin of"Location San Jiao",analyze the origin of its argument,and discuss the problems in it,so as to preliminarily clarify the connotation of San Jiao conception.Through investigation,it is found that the"Location San Jiao"is actually the dissimilation of"Liu Fu San Jiao",and the three parts of"Liu Fu San Jiao"should be divided by functional features rather than anatomical structure.

Discussion on "Wei Qi Chu Shang Jiao"

Due to the different versions of Nei Jing have been published in the past,variant of records have shown the different explanation of the original source of Wei Qi,thus there are two popular options are still under debate,Shang Jiao and Xia Jiao.Our research has found that the core reason of above dispute is caused by the vague understanding of the meaning of"Chu".Based on the analysis of relevant text of Nei Jing and the character of Chu with the meaning of leaving,it is concluded that"Wei Qi Chu Shang Jiao".And it means Wei Qi has left Shang Jiao of Zang Fu system and following into Meridian system,according to Wei Qi's connotation of following and protecting.Shang Jiao is the original starting point of Wei Qi in circulation just as it is born.And Shang Jiao is the place where"the slippery and tough Qi of water and grain"has transformed its name into"Wei Qi",which is also the conjunction point that Wei Qi changing from Ti(ontology)into Yong(methodology).And it explains the theory of clinical application based on this view.

Literature and Time:On Jiao Xun’s Concept of Literary Transformation

Jiao Xun’s concept of the succession of literary genres according to each generation’s strengths clearly establishes the interrelationship between historical time and literary genres.Jiao Xun’s idea of the transformation of literary styles differs from the prevailing notion at that time,which advocated prioritizing poetry and prose that conveyed aspirations and ideals while considering other literary genres such as ci(a form of lyric poetry),qu(drama),and novels as inferior.Instead,Jiao Xun advocated using the“nature”and“events”of objects as criteria and awakened a more traditional“poetic teaching tradition”characterized by metaphorical expression and emotional resonance,which aligned with the concept of literature conveying aspirations and ideals.This enabled the standard of“nature”to transcend the boundaries of literary genres,establishing new criteria for the hierarchy of genres and encapsulating Jiao Xun’s intention to summon emerging literary genres that were in harmony with the“present”.

The Idea of Edification in Jiao Xun’s Conception of Drama

Jiao Xun’s drama edification concept emphasizes“morality”and“emotion”at the same time,which expresses his tendency for drama conception to move closer to classics.Jiao Xun thinks that drama creation values the audience’s acceptance,which also makes drama an effective testing ground for re-processing the relationship between“morality”and“emotion”in Confucianism;and Jiao Xun respects folk daily life in popular literary forms to promote moral enlightenment,which shows that drama research has become one of his practical methods of“changing customs”.

A Review on Ben Cao Gang Mu Ying Jiao Dui Zhao(《本草纲目影校对照》A Contrast between the Photocopy and Collation of Compendium of Materia Medica)

Ben Cao Gang Mu Ying Jiao Dui Zhao(《本草纲目影校对照》A Contrast between the Photocopy and Collation of Compendium ofMateria Medica),completed by Zhang Zhibin and Zheng Jinsheng,is the latest variorum on Ben Cao Gang Mu(《本草纲目》Compendium ofMateria Medica).Adopting five different Jinling editions as the master copy,the book carefully collates the complex contents on the original book by contrasting the photocopies with the supplement and correction,which makes it easy to use.What is more,full-form punctuation is used to mark the reference books and proper nouns,which is a big success feat in the research history of Ben Cao Gang Mu.

ChronologicalInferenceofPuSongling。SPoems＂TOBeIII，SicknessFoot，andNiannujiao

路编《蒲松龄年谱》谓蒲氏《抱病》等诗写于康熙十八年，盛编《蒲松龄年谱》谓《病足》等诗写于康熙二十五年，袁世硕先生谓蒲袁酬答词全在康熙二十四年。其实，蒲氏《抱病》、《病足》、《念奴娇·新秋月夜，病中感赋，呈袁宣四孝廉》等作品，均有可能写于康熙十八年，“抱病”和“病足”说的实际是一回事。因此，有关聊斋诗词的编年问题，应该重新考虑。

Effect of Jiaotai Pill(交泰丸)on Intestinal Damage in Partially Sleep Deprived Rats

Objective: To explore the effect and mechanism of Jiaotai Pill(交泰丸, JTW) on intestinal mucosal damage in rats with chronic partial sleep deprivation(PSD). Methods: Obesity resistant(OR) rats were selected, and underwent 4 h PSD by being exposed to environmental noise for 4 weeks. During the whole PSD period, JTW and estazolam were orally given to the rats respectively in the treating groups. Plasma concentration of lipopolysaccharide(LPS) which is the marker of gut-origin endotoxemia was examined. Intestinal morphology changes were observed by optical microscopy. The protein expression of occludin(Ocln) in the intestine was measured by immunofluorescence technique and Western blot. The expressions of circadian proteins cryptochromes(Cry1 and Cry2) in the intestine were also determined. Results: The treatment of JTW significantly decreased LPS level in OR rats with PSD(P<0.05). JTW also attenuated insomnia-induced intestinal injury like shorter, sparse and incomplete villus, wide gap between the villus, mucosal swelling and congesting(P<0.05). These changes were associated with the effect of JTW on up-regulating the expressions of Cry1 protein, Cry2 protein and Ocln protein in the intestine. Conclusions: JTW has the beneficial effect on improving intestinal mucosal damage caused by PSD. The mechanism appears to be related to the modulation of the expressions of circadian proteins and Ocln protein in the intestine, thereby attenuating inflammation and improving insulin resistance in insomnia rats.

Measurement and research method of metallogenic depths of Jiaojia orefield and Linglong orefield, Shandong Province, China

Taking the contribution of the tectonic force to the total hydrostatic pressure into account, the author puts forward a new method on the calculation of the depth of petrogenesis and metallogenesis which is summarized as follows: first the tectonic added hydrostatic pressured Ps is subtracted from the total hydrostatic pressure P, then using their difference Pr, according to the general method the depth of petrogenesis and metallogenesis can be determined in consideration of lateral constraint.By the new method the following data on the depths of the metallogenesis are obtained: 2 243.6 m (No. I and No. II veins with metallogenic epoch of 105 Ma) and 1 632.38 m (No. III vein with 105 Ma) for Jiaojia orefield, and 3 454.97 m (NE-trending zone with 213.2 Ma), 1 902.79 m (ENE-trending zone with 100.28 Ma), 1 090.97 m (NE-trending zone with 80.67 Ma) and 720.55 m (NNE-trending zone with 71.86 Ma) for Linglong orefield.

Jiao Shao: A forerunner of physiological psychology and comparative psychology in China

Jiao Shao (邵郊,1923-2017)(Fig. 1), who was an expert in physiological psychology and comparative psychology in China, made important contributions to the development of physiological psychology and comparative psychology, scientific research and personnel training (The obituary of Jiao Shao, 2017). He was a member of the Second Discipline Review Group of the State Council (Pedagogy), the director of the third and fifth sessions of Chinese Psychological Society (CPS), the chairman of the Professional Committee on Physiological Psychology of the CPS (1984-1988), an editorial advisory board member of the Encyclopedia of China Publishing House (First Edition), an editorial board member of Acta Psychologica Sinica (1979-1991), and he was honored as a fellow of the CPS (2007).

Structure, evolution and regional tectonic implications of the Queshan metamorphic core complex in eastern Jiaodong Peninsula of China

The Queshan MCC is an important example of a crustal extensional structure in the eastern Jiaodong Peninsula along the southeastern margin of the NCC in the Early Cretaceous. The MCC is a typical Cordilleran-type core complex with a three-layered structure:(1) the upper plate is constituted by the Cretaceous supradetachment basin and Paleoproterozoic basement;(2) the lower plate comprises the Neoarchean high-grade metamorphic complexes and late Mesozoic granitic intrusions; and(3) the two plates are separated by a master detachment fault. A series of late NEN-oriented brittle faults superimposed on and destructed the early MCC. Petrology, geometry, kinematics, macro- and micro-structures and quartz c-axis fabrics imply that the MCC has a progressive exhumation history from middle-lower to subsurface level(via middle-upper crustal level) under the nearly WNW-ESE regional extensional regime. We present structural and geochronological evidence to constrain the exhumation of the Queshan MCC from ca. 135 to 113 Ma. Based on the comprehensive analysis of the different patterns of extensional structures in the Jiaodong and Liaodong Peninsula, we have defined the Jiao-Liao Early Cretaceou extensional province and further divided the crustal extension of it into two stages: the first stage was the intense flow of the middle-lower crust and the second stage was the extension of the middle-upper crust. Combining the tectonic setting, the lithosphere thinning in the Jiao-Liao Early Cretaceous extensional province can be considered a typical model for the response of crust-mantle detachment faulting under regional extension in East Asia.