OBJECTIVE: To evaluate the anti-tumor activity of ethyl acetate fraction(EFA), extracted with ethanol from the root of "Dai-Bai-Jie" in A549 cancer cells and its underlying mechanism.METHODS: "Dai-Bai-J...OBJECTIVE: To evaluate the anti-tumor activity of ethyl acetate fraction(EFA), extracted with ethanol from the root of "Dai-Bai-Jie" in A549 cancer cells and its underlying mechanism.METHODS: "Dai-Bai-Jie" was extracted with 95%ethanol-aqueous(DBJ-1), 50% ethanol-aqueous(DBJ-2), and water(DBJ-3) by reflux method. 95%ethanol-aqueous extract was separated byethyl acetate(EFA) and n-butyl alcohol(DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related pro-teins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity in vivo.RESULTS: DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC5025 and 3.5 μg/mL, respectively. EFA can exhibit the proliferation,arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells in vitro. The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the host.CONCLUSION: EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53,Caspase-3, and Caspase-8 proteins.展开更多
Lung cancer is the most malignant tumor disease with the highest diagnosis and mortality rate in China.The development of therapeutic drugs is the current research focus.Dai-Bai-Jie is a traditional medicine of the Da...Lung cancer is the most malignant tumor disease with the highest diagnosis and mortality rate in China.The development of therapeutic drugs is the current research focus.Dai-Bai-Jie is a traditional medicine of the Dai nationality,which is commonly used in the treatment of decreasing swelling,alleviating pain and detoxification.Most of the current researches focused on the component analysis of Dai-Bai-Jie,but few researches studied on its antitumor and pharmacological effect.In this study,we incubated A549 cells with different concentrations of Dai-Bai-Jie.The cell proliferation experiment showed that the DaiBai-Jie solution inhibited the proliferation of A549 cells and caused cell apoptosis.In this work,we confirmed that Dai-Bai-Jie had an inhibitory effect on the proliferation and migration of non-small cell lung cancer A549,which may be used as a novel candidate of anti-tumor therapy for lung cancer patients.展开更多
基金Supported by the Grant from the Basic Scientific Research Special of the Central Public Welfare Research Institutes of IMPLADChinese Academy of Medical Sciences:Study on anti-lung cancer chemical constituents and mechanism of"Dai-Bai-Jie"(No.YZYN1501)Study on the resources of"Dai-Bai-Jie"(No.YZYN-10-02)
文摘OBJECTIVE: To evaluate the anti-tumor activity of ethyl acetate fraction(EFA), extracted with ethanol from the root of "Dai-Bai-Jie" in A549 cancer cells and its underlying mechanism.METHODS: "Dai-Bai-Jie" was extracted with 95%ethanol-aqueous(DBJ-1), 50% ethanol-aqueous(DBJ-2), and water(DBJ-3) by reflux method. 95%ethanol-aqueous extract was separated byethyl acetate(EFA) and n-butyl alcohol(DBJ-5), consecutively. The SRB method was used to evaluate the cytotoxic activity. Annexin V-FITC staining was applied to observe the apoptosis and analyze the cell cycle activated by EFA in A549 tumor cell. Western blot was used to detect the apoptosis/related pro-teins expressions. A549 tumor cellsbearing nude mice model was employed to measure the tumor volume, mice weight, and tumor inhibition ratio in order to verify the antitumor activity in vivo.RESULTS: DBJ-1 and EFA showed better cytotoxic activity on A549 tumor cells with IC5025 and 3.5 μg/mL, respectively. EFA can exhibit the proliferation,arrest cell cycle at G0/G1 phase, and induce apoptosis in A549 tumor cells in vitro. The mechanisms of apoptosis induced by EFA may be associated with decreasing Bcl-2 protein expression and increasing p53, Bax, Caspase-3, and Caspase-8 proteins expression. EFA also possessed significant anti-tumor efficacy in nude mice, and little toxicity was observed in the host.CONCLUSION: EAF could induce A549 tumor cells apoptosis and G0/G1 cell cycle arrest. A549 tumor cells apoptosis induced by EAF may be associated with the decrease in the ratio of Bcl-2 and Bax mRNA levels, and increase in the expression of p53,Caspase-3, and Caspase-8 proteins.
基金supported by the National Key R&D Program of China(No.2017YFC0906800)National Natural Science Foundation of China(Nos.21435002 and 21621003)。
文摘Lung cancer is the most malignant tumor disease with the highest diagnosis and mortality rate in China.The development of therapeutic drugs is the current research focus.Dai-Bai-Jie is a traditional medicine of the Dai nationality,which is commonly used in the treatment of decreasing swelling,alleviating pain and detoxification.Most of the current researches focused on the component analysis of Dai-Bai-Jie,but few researches studied on its antitumor and pharmacological effect.In this study,we incubated A549 cells with different concentrations of Dai-Bai-Jie.The cell proliferation experiment showed that the DaiBai-Jie solution inhibited the proliferation of A549 cells and caused cell apoptosis.In this work,we confirmed that Dai-Bai-Jie had an inhibitory effect on the proliferation and migration of non-small cell lung cancer A549,which may be used as a novel candidate of anti-tumor therapy for lung cancer patients.
