Objective:The aim of our study was to investigate whether Jinshuixian has the abilities of inhibiting tumor growth and radio-sensitivity effect.Methods:Cultured lung A549 cells were randomly divided into 6 groups:norm...Objective:The aim of our study was to investigate whether Jinshuixian has the abilities of inhibiting tumor growth and radio-sensitivity effect.Methods:Cultured lung A549 cells were randomly divided into 6 groups:normal control group(NC),the Jinshuixian group(JSX),radiotherapy(RT),JSX for the first day and the next day followed by RT group(JSX→RT),RT for the first day and the next day followed by JSX group(RT→JSX)and RT+JSX concomitantly group(JSX+RT).MTT was applied to measure the cell viability,RT-PCR and ELISA were used to test the expression of mRNA and protein of VEGF.Results:The proliferation of A549 cells were inhibited in JSX and combined groups and the inhibiting effects were time dependent.The expression of VEGF in RT group was increased,however,VEGF in JSX and combination groups were largely decreased over time when compared to NC group.Results in JSX→RT,RT→JSX and JSX+RT groups did not achieve significantly differences.Conclusion:JSX has the ability of anti-tumor growth in vitro accompanied down-regulating of VEGF,especially when combined with radiotherapy,and its effect is time-dependent.However,more studies in vivo and in vitro are needed to further supporting these effects.展开更多
文摘Objective:The aim of our study was to investigate whether Jinshuixian has the abilities of inhibiting tumor growth and radio-sensitivity effect.Methods:Cultured lung A549 cells were randomly divided into 6 groups:normal control group(NC),the Jinshuixian group(JSX),radiotherapy(RT),JSX for the first day and the next day followed by RT group(JSX→RT),RT for the first day and the next day followed by JSX group(RT→JSX)and RT+JSX concomitantly group(JSX+RT).MTT was applied to measure the cell viability,RT-PCR and ELISA were used to test the expression of mRNA and protein of VEGF.Results:The proliferation of A549 cells were inhibited in JSX and combined groups and the inhibiting effects were time dependent.The expression of VEGF in RT group was increased,however,VEGF in JSX and combination groups were largely decreased over time when compared to NC group.Results in JSX→RT,RT→JSX and JSX+RT groups did not achieve significantly differences.Conclusion:JSX has the ability of anti-tumor growth in vitro accompanied down-regulating of VEGF,especially when combined with radiotherapy,and its effect is time-dependent.However,more studies in vivo and in vitro are needed to further supporting these effects.
