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Clinical significance of serum intercellular adhesion molecule-1 detection in patients with hepatocellular carcinoma 被引量:20
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作者 Ming Hui Mei Jing Xu Qin Fen Shi Jing Hong Yang Qian CHen Li Ling Qin Department of Hepatobiliary Surgery, Institute of Hepatobiliary Surgery,Guilin Medical College,Guilin 541001,Guangxi Province,China 《World Journal of Gastroenterology》 SCIE CAS CSCD 2000年第3期408-410,共3页
INTRODUCTION Since the late 1980s,studies on the expression ofintercellular adhesion molecule-1(ICAM-1)inpatients with malignancies have demonstrated thatICAM-1 may strongly express in two forms in suchdiseases:membra... INTRODUCTION Since the late 1980s,studies on the expression ofintercellular adhesion molecule-1(ICAM-1)inpatients with malignancies have demonstrated thatICAM-1 may strongly express in two forms in suchdiseases:membranous one on the surface of tumorcells(membrane-bound ICAM-1)and soluble one incirculation(soluble ICAM-1,sICAM-1). 展开更多
关键词 carcinoma hepatocellular/diagnosis INTERCELLULAR adhesion molecule-1 liverneoplasms/diagnosis ALPHA-FETOPROTEIN
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The Value of the Soluable Intercellular Adhesion Molecule-1 Levels in Matermal Serum for Determination of Occult Chorioamnionitis in Premature Rupture of Membranes 被引量:6
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作者 邹丽 张会军 +1 位作者 祝建芳 朱剑文 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2004年第2期154-157,共4页
To compare the diagnostic value of soluble intercellular adhesion molecule 1 (sICAM-1) with that of c-reactive protein (CRP) for detecting chorioamnionitis (CAM) in serum of women with premature rupture of membranes (... To compare the diagnostic value of soluble intercellular adhesion molecule 1 (sICAM-1) with that of c-reactive protein (CRP) for detecting chorioamnionitis (CAM) in serum of women with premature rupture of membranes (PROM), 55 pregnant women with PROM, including 18 pregnant women with preterm premature rupture of membranes (PPROM) and 20 normal pregnant women at term (TPROM) were studied. Maternal serum were measured by Sandwish enzyme-linked immunoabsorbent assay (ELISA) for sICAM. CAM was histologically confirmed after delivery. The results revealed that (1) maternal serum levels of sICAM-1 and CRP were significantly higher in women with PROM than those without it; (2) maternal serum levels of sICAM-1 and CRP were significantly higher in women with CAM than those without it; (3) serum levels of sICAM-1 in PPROM women were similar to those in TPROM women, whereas serum levels of CRP in PPROM women were significantly higher than those in TPROM women; (4) the sensitivity, specificity, positive predictive value, negative predictive value, Kappa index and area under receiver operating characteristic (ROC) curve of maternal serum sICAM-1 (cutoff 104.7 ng/ml) and CRP(cutoff 1.03 mg/dl) for diagnosing CAM were 100 %, 91.2 %, 87.5 %, 100 %, 0.20, 0.995 and 81.0 %, 73.5 %, 65.4 %, 86.2 %, 0.13, 0.811, respectively; (5) among the mild histological CAM group, severe histological CAM group and clinical CAM group, the difference in maternal serum levels of sICAM-1 were significantly (P<0.001), with the order of concentration from high level to low level corresponding to the severity of CAM. It is concluded that maternal serum level of ICAM-1 is superior to that of CRP as biomarker for diagnosing intraamniotic infection in pregnant women with PROM. 展开更多
关键词 intercellular adhesion molecule-1 chorioamninitis diagnosis
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Effects of TCMP-1 on the changes of platelet endothelial cell adhesion molecule-1 expression in acute edematous pancreatitis 被引量:4
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作者 Hong-Kai Gao, Zong-Guang Zhou, Tao He, You-Qin Chen, Fang-Hai Han and Cun Wang Chengdu, ChinaDepartment of General Surgery & Division of Diges- tive Surgery, West China Hospital, Sichuan University, Chengdu 610041 , China 《Hepatobiliary & Pancreatic Diseases International》 SCIE CAS 2004年第2期311-315,共5页
BACKGROUND: Traditional Chinese medicine is a potent agent in the management of clinical and experimental acute pancreatitis (AP), but the molecular mechanism of its the- rapeutic action is unclear. Numerous experimen... BACKGROUND: Traditional Chinese medicine is a potent agent in the management of clinical and experimental acute pancreatitis (AP), but the molecular mechanism of its the- rapeutic action is unclear. Numerous experimental and clinical studies have shown that platelet endothelial cell ad- hesion molecule-1 (PECAM-1) is pivotal to leukocyte re- cruitment, which results in microcirculatory injury during inflammation, but its role in acute pancreatitis is poorly un- derstood. We investigated the effects of a compound of tra- ditional Chinese medicine pancreatitis-1 (TCMP-1) on the changes of platelet endothelial cell adhesion molecule-1 (PECAM-1) expression on polymorphonuclear leukocytes (PMNs) in acute edematous pancreatitis (AEP). METHODS: The model of acute pancreatitis was estab- lished by subcutaneous injection of caerulein, and TCMP-1 treated groups were given TCMP-1 by catheterization from mouth to stomach (20 ml/kg) immediately after first time subcutaneous injection of caerulein. The changes of expres- sion of PECAM-1 on leukocytes from the blood of the splenic vein and inferior vena cava were determined by flow cytometry. RESULTS: In the AEP group, expression of PECAM-1 on PMNs was not significantly different between pancreatic microcirculation and systemic circulation at AEP2h and AEP4h time point. Then from AEP4h time point to AEP8h time point, expression of PECAM-1 was up-regulated in systemic circulation while it was down-regulated in pancre- atic microcirculation and was significantly different be- tween pancreatic microcirculation and systemic circulation at AEP8h time point (P<0.05). In the TCMP-1 treated group, compared with the AEP group, expression of PE-CAM-1 on PMNs decreased in different levels between pan- creatic microcirculation and systemic circulation and was of significant difference at AEP8h time point (P <0.05). CONCLUSION: Inhibition of PECAM-1 expression on PMNs may prevent PMNs from transmigration through the endo- thelium and may be one of the treatment mechanisms of TCMP-1 decoction on AEP. 展开更多
关键词 acute pancreatitis platelet endothelial cell adhesion molecule-1 Chinese medicine
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The relationship between platelet endothelial cell adhesion molecule-1 and paraquat-induced lung injury in rabbits 被引量:7
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作者 Jing Shi Chun-lin Hu +2 位作者 Yu-feng Gao Xiao-xing Liao Hope Xu 《World Journal of Emergency Medicine》 CAS 2012年第1期60-64,共5页
BACKGROUND:Platelet endothelial cell adhesion molecule-1(PECAM-1),also known as CD31,is mainly distributed in vascular endothelial cells.Studies have shown that PECAM-1 is a very significant indicator of angiogenesis,... BACKGROUND:Platelet endothelial cell adhesion molecule-1(PECAM-1),also known as CD31,is mainly distributed in vascular endothelial cells.Studies have shown that PECAM-1 is a very significant indicator of angiogenesis,and has been used as an indicator for vascular endothelial cells.The present study aimed to explore the relationship between the expression of PECAM-1 and the degree of acute lung injury(ALI) and fibrosis in paraquat(PQ) induced lung injury in rabbits.METHODS:Thirty-six adult New Zealand rabbits were randomly divided into three groups(12rabbits in each group) according to PQ dosage:8 mg/kg(group A),16 mg/kg(group B),and 32 mg/kg(group C).After PQ infusion,the rabbits were monitored for 7 days and then euthanized.The lungs were removed for histological evaluation.Masson staining was used to determine the degree of lung fibrosis(LF),and semi-quantitative immune-histochemistry analysis to determine the expression of PECAM-1.Pearson's product-moment correlation analysis was performed to evaluate the relationship between the expression of PECAM-1 and the extent of lung injuries expressed by ALI score and degree of LF.RESULTS:Rabbits in the three groups showed apparent poisoning.The rabbits survived longer in group A than in groups B and C(6.47±0.99 days vs.6.09±1.04 days vs.4.77±2.04 days)(P<0.05).ALI score was lower in group A than in groups B and C(8.33±1.03 vs.9.83±1.17 vs.11.50±1.38)(P<0.05),and there was statistically significant difference between group B and group C(P=0.03).LF was slighter in group A than in groups B and C(31.09%±2.05%vs.34.37%±1.62%vs.36.54%±0.44%)(P<0.05),and there was statistically significant difference between group B and group C(P=0.026).The PEACAM-1 expression was higher in group A than in groups B and C(20.31%±0.70%vs.19.34%±0.68%vs.18.37%±0.46%)(P<0.05),and there was statistically significant difference between group B and group C(P=0.017).Pearson's correlation analysis showed that the expression of PECAM-1 was negatively correlated to both ALI score(Coe=-0.732,P=0.001)and degree of LF(Coe=-0.779,P<0.001).CONCLUSIONS:The PECAM-1 expression significantly decreases in New Zealand rabbits after PQ poisoning,and the decrease is dose-dependent.The PECAM-1 expression is negatively correlated with ALI score and LF,showing a significant role in the development of lung injuries induced by PQ. 展开更多
关键词 Platelet endothelial cell adhesion molecule-1 PARAQUAT Acute lung injury Lung fibrosis
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Mild hypothermia effects on intercellular adhesion molecule-1 and serum interleukin-6 expression in brain tissues of a rat focal ischemia model 被引量:1
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作者 Shengqi Fu Lei Yang +2 位作者 Shuling Zhang Shilong Sun Xingai Mao 《Neural Regeneration Research》 SCIE CAS CSCD 2008年第10期1124-1127,共4页
BACKGROUND: Previous studies have confirmed the neuroprotective effect of mild hypothermia on ischemic brain injury. OBJECTIVE: To investigate the effects of mild hypothermia on intercellular adhesion molecule-1 exp... BACKGROUND: Previous studies have confirmed the neuroprotective effect of mild hypothermia on ischemic brain injury. OBJECTIVE: To investigate the effects of mild hypothermia on intercellular adhesion molecule-1 expression and serum interleukin-6 levels in ischemic brain tissues of focal brain ischemia rats, and to explore the neuroprotective effects of mild hypothermia on ischemic brain injury. DESIGN, TIME AND SETTING: A randomized, controlled, neurobiological experiment was performed at the Central Laboratory, First Affiliated Hospital, Xinxiang Medical College, China from February to July 2006. MATERIALS: Thirty healthy, adult, Sprague Dawley rats were used to establish middle cerebral artery occlusion models using the suture method, The immunohistochemistry (streptavidin-biotin-peroxidase complex method) kit was purchased from Boster, China. Interleukin-6 radioimmunoassay was supplied by Institute of Radioimmunity, Technology Development Center, General Hospital of Chinese PLA. METHODS: The rats were equally and randomly assigned into mild hypothermia and control groups, and middle cerebral artery occlusion models were established. The rectal temperature was maintained at (37 ±0.5)℃ in the control group. In the mild hypothermia group, the rectal temperature was maintained at (33±1)℃. MAIN OUTCOME MEASURES: At 12 hours after model establishment, the ischemic brain hemispheres were coronally sliced at the level of the optic chiasm. The number of intercellular adhesion molecule-1-positive vessels per high-power field was observed with an optical microscope. Serum interleukin-6 levels were measured by radioimmunoassay. RESULTS: Compared with the control group, intercellular adhesion molecule-1 and serum interleukin-6 expressions were significantly decreased in ischemic brain tissues of the mild hypothermia group (P 〈 0.01). CONCLUSION: Mild hypothermia exhibits a neuroprotective effect by reducing serum interleukin-6 and intercellular adhesion molecule-1 expression following cerebral ischemia. 展开更多
关键词 cerebral ischemia intercellular adhesion molecule-1 INTERLEUKIN-6 mild hypothermia
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Association of G+1688A Polymorphism of Platelet Endothelial Cell Adhesion Molecule-1 Gene with Myocardial Infarction in the Chinese Han Population 被引量:1
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作者 杨颖 程龙献 +3 位作者 Ripen Nsenga 何美安 常智堂 邬堂春 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2007年第5期520-523,共4页
In order to investigate the association of G+1688A (Ser563Asn) polymorphism of platelet endothelial cell adhesion molecule-1 (PECAM-1) gene with myocardial infarction (MI) in the Chinese Han population, the G+... In order to investigate the association of G+1688A (Ser563Asn) polymorphism of platelet endothelial cell adhesion molecule-1 (PECAM-1) gene with myocardial infarction (MI) in the Chinese Han population, the G+1688A polymorphism in PECAM-1 gene was detected by polymerase chain reaction-restriction fragment-length polymorphism (PCR-RFLP) method among 502 subjects, including 218 patients with MI and 284 controls. The results showed that there was significant difference in AA frequencies of genotype G+1688A polymorphism between case and control groups (39% vs 24%, P〈0.001). A similar trend was observed on the allele frequencies (A/G: 62% vs 49%, P〈0.001). Among the subjects with high serum total cholesterol level or high systolic blood pressure level, the variant AA genotype was associated with high risk of MI (adjusted OR, 2.13; 95% CI, 1.08 -4.41 and adjusted OR, 2.53; 95%CI, 1.63-3.63). The single nucleotide polymorphism (SNP) at position +1688 in the exon 8 of PECAM-1 gene was associated with MI and the allele A might be a risk factor for MI in the Chinese Han population. 展开更多
关键词 platelet endothelial adhesion molecule-1 single nucleotide polymorphism myocardial infarction polymerase chain reaction-restriction fragment-length polymorphism
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Expression of Intercellular Adhesion Molecule-1 in Immune Response of Inner Ear
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作者 龚树生 曾新力 +1 位作者 严臻 汪吉宝 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 1998年第4期208-211,共4页
To understand the role of intercellular adhesion molecule-1 (ICAM-1) in immune response of the inner ear, inner ear immune response was induced in rats by inoculation of keyhole limpet hemocyanine (KLH) into the scala... To understand the role of intercellular adhesion molecule-1 (ICAM-1) in immune response of the inner ear, inner ear immune response was induced in rats by inoculation of keyhole limpet hemocyanine (KLH) into the scala tympani of the animals who had been systemically sensitized. The expression of ICAM-1 in the inner ear was immunohistochemically examined. ICAM-1 was found in the epithelium of the spiral modiolar vein (SMV) with its collecting venules (CVs) as early as 6 h after challenge. Expression of ICAM-1 was observed on the epithelium of the endolymphatic sac (ES) and perisaccular region at 12 h. The intensity of ICAM-1 staining reached its peak within 24 - 48 h in these sites of the inner ear. By day 28, most specimens were devoid of appreciable staining for ICAM-1. Our study demonstrates that adhesion molecules play an important role in extravasation of inflammatory cells from the systemic circulation in the process of inner ear immune response. It also shows that cytokines that control expression of adhesion molecules may be released by cells outside ES, besides those cells in the ES. 展开更多
关键词 LABYRINTH AUTOIMMUNITY intercellular adhesion molecule-1 en-dolymphatic sac
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Changes of soluble intercellular adhesion molecule-1 in the serum from patients with acute cerebral infarction
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作者 周华东 陈曼娥 颜建华 《Journal of Medical Colleges of PLA(China)》 CAS 1997年第4期328-330,共3页
objective: To observe the changes of soluble intercellular adhesion molecule-1(sICAM-1) in the serum of patients with acute cerebral infarctlon (ACI) and their clinical significance. Methods: The concen-tration of sIC... objective: To observe the changes of soluble intercellular adhesion molecule-1(sICAM-1) in the serum of patients with acute cerebral infarctlon (ACI) and their clinical significance. Methods: The concen-tration of sICAM-1 in the serum of 91 patients with ACI was determined with ELISA and then the results were compared wlth those of 43 patients with cerebral hemorrhage and 30 healthy individuals. Results: In the 24th hour after infarction. the concentration of sICAMu-1 in the serum was significantly higher in patients with ACI than in patients with cerebral hemorrhage and normal controls (P< 0. 01). In the patients with ACI, the concentration exhibited an decreasing tendency in the period from the 24th hour to the 14th day andwas correlated with the focal size of cerebral infarction. During the first 14 days after infarction, the concen-tration was significantly higher in the patients with the complication of infection than in those without. Con-clusion: sICAM-1 is closely correlated with clinical manifestation of ACI. 展开更多
关键词 CEREBRAL INFARCTION SOLUBLE INTERCELLULAR adhesion molecule-1 (sICAM-1)
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Relationship of plasma homocysteine, soluble intercellular adhesion molecule-1, high mobility group box 1 protein with carotid intima-media thickness in elderly patients with type 2 diabetes mellitus
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作者 Zhijie Cai Jun Xue +4 位作者 Xiaohui Ma Peifeng Chen Biao Ge Yuying Zhang Zhihui Dong 《Discussion of Clinical Cases》 2022年第1期9-12,共4页
Objective:To explore the relationship of plasma homocysteine(Hcy),soluble intercellular adhesion molecule-1(sICAM-1)and high mobility group box 1 protein(HMGB1)with carotid intima-media thickness(c-IMT)in elderly pati... Objective:To explore the relationship of plasma homocysteine(Hcy),soluble intercellular adhesion molecule-1(sICAM-1)and high mobility group box 1 protein(HMGB1)with carotid intima-media thickness(c-IMT)in elderly patients with type 2 diabetes mellitus.Methods:A total of 100 elderly patients who were diagnosed as type 2 diabetes mellitus in Baogang Hospital of Inner Mongolia from June 2017 to May 2020 were chosen as research objects.According to c-IMT,they were divided into the normal group(n=35),the mild to moderate group(n=41)and the severe group(n=24).The expression levels of plasma Hcy,sICAM-1 and HMGB1 were compared between groups respectively.Pearson’s correlation coefficient was used to analyze the relationship of plasma Hcy,sICAM-1,HMGB1 with c-IMT.Results:The comparison in plasma Hcy,sICAM-1,HMGB1 and c-IMT among the three groups of patients was of statistical significance(p<.05).The results of correlation analysis showed that the expression levels of plasma Hcy,sICAM-1 and HMGB1 were positively correlated with c-IMT in elderly patients with type 2 diabetes mellitus(r=.627,.598,.614;p<.05).Conclusions:The expression levels of plasma Hcy,sICAM-1 and HMGB1 are abnormally increased in elderly patients with type 2 diabetes mellitus,and related to c-IMT,which can provide a strong evidence for clinical diagnosis and treatment by detecting their levels in clinical practice. 展开更多
关键词 Type 2 diabetes mellitus Carotid intima-media thickness HOMOCYSTEINE Soluble intercellular adhesion molecule-1 High mobility group box 1 protein
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The effects of coxsackievirus B3 infection on induction of ICAM-1 in cardiac myocytes and on Iymphocyte-myocyte adhesion
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作者 沈茜 徐雨莲 +1 位作者 林伟 吴红 《Journal of Medical Colleges of PLA(China)》 CAS 1999年第3期205-208,共4页
关键词 coxsackievirus B INTERCELLULAR adhesion molecule-1 LYMPHOCYTE
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高危型人乳头瘤病毒感染患者宫颈脱落细胞JAM3/PAX1高甲基化诊断宫颈高级别病变 被引量:1
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作者 李翔 贺斯黎 +4 位作者 赵行平 孙丹 吴思 徐大宝 李颖佳 《中南大学学报(医学版)》 CAS CSCD 北大核心 2023年第12期1820-1829,共10页
目的:传统的宫颈癌筛查手段——高危型人乳头瘤病毒(high-risk human papillomavirus,hrHPV)和宫颈脱落细胞学检测存在局限性。本研究通过检测宫颈脱落细胞中连接黏附分子3(junctional adhesion molecule 3,JAM3)/配对盒基因1(paired bo... 目的:传统的宫颈癌筛查手段——高危型人乳头瘤病毒(high-risk human papillomavirus,hrHPV)和宫颈脱落细胞学检测存在局限性。本研究通过检测宫颈脱落细胞中连接黏附分子3(junctional adhesion molecule 3,JAM3)/配对盒基因1(paired box gene 1,PAX1)高甲基化水平,并将其与液基薄层细胞学(liquid based cytology,LBC)进行比较,评价JAM3/PAX1甲基化对于宫颈高级别病变的诊断能力,以探索新的宫颈高级别病变的诊断模式,实现宫颈高级别病变“精准筛查”的目的。方法:回顾性收集2021年6月至2022年6月在中南大学湘雅三医院妇科阴道镜门诊接受检查的患者共136例,包括宫颈非高级别病变122例,高级别病变14例。研究的变量包括:基本临床信息(年龄、体重指数、是否绝经)、LBC、hrHPV、宫颈组织病理、阴道微生态结果、阴道镜结果(宫颈转化区类型)、JAM3(ΔCtJ)和PAX1(ΔCtP)基因甲基化的ΔCt值。首先通过logistic回归分析筛选影响宫颈高级别病变的影响因素,并进行相关性分析,然后用差异有统计学意义的变量构建条件推断树模型。结果:Logistic回归分析提示高级别宫颈病变组与非高级别宫颈病变组的PAX1与JAM3基因甲基化ΔCt值以及LBC检测结果差异均有统计学意义(均P<0.05)。相关性分析发现,宫颈病理结果与ΔCtP(r=-0.360,P<0.001)、ΔCtJ(r=-0.448,P<0.001)、LBC(r=-0.305,P<0.001)、菌群多样性(r=-0.183,P=0.037)呈负相关。条件推断树显示:当ΔCtJ>10.13时,全部为宫颈非高级别病变的患者;当ΔCtP>6.22时,非高级别病变的患者有117例,占97.5%,高级别病变者仅3例,占2.5%。ΔCtJ>8.61且LBC为不明确的非鳞状上皮细胞(atypical squamous cell of undetermined significance,ASC-US)或未见上皮内病变细胞时,105例(99.1%)为宫颈非高级别病变的患者,仅1例(0.9%)检出为高级别病变;当LBC结果为高级别病变时,仅9例患者检出高级别病变,3例检出非高级别病变;而当LBC提示低级别病变、ASC-US、未见上皮内病变细胞,且ΔCtP>6.22时,有117例(97.5%)的患者检出非高级别病变。结论:在人乳头瘤病毒感染妇女中,JAM3/PAX1双基因甲基化检测可独立应用于宫颈高级别病变/非高级别病变的分层诊断,且不依赖于宫颈脱落细胞学检测结果;亦可与LBC联合使用以弥补LBC敏感度低的缺点。此外,未来将甲基化试剂盒应用于大规模宫颈癌筛查,有利于发现更多宫颈高级别病变患者,并达到早筛、早治宫颈病变/癌的目的。 展开更多
关键词 宫颈病变 DNA甲基化 连接黏附分子3 配对盒基因1 条件推断树
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Intercellular Adhension Molecule-1 in the Pathogenesis of Heroin-induced Acute Lung Injury in Rats 被引量:3
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作者 周琼 白明 邹世清 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2004年第5期430-432,共3页
The expression of intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of heroin-induced acute lung injury (ALI) in rats was investigated. The model of ALI was established by intravenous injection of heroin ... The expression of intercellular adhesion molecule-1 (ICAM-1) in the pathogenesis of heroin-induced acute lung injury (ALI) in rats was investigated. The model of ALI was established by intravenous injection of heroin into tail vein in rats. Thirty-six rats were randomly divided into heroin-treated groups (1 h, 2 h, 4 h, 6 h and 24 h) and normal control group. Changes in histopathologic morphology and biological markers of ALI were measured. The expression of ICAM-1 in lung tissue was detected by using immunohistochemistry and RT-PCR. The results showed that the W/D ratio and protein contents in BALF of the heroin-treated groups were significantly higher than that of the control group (P<0.01). The histopathological changes in the lung tissue were more obvious in heroin-treated groups. The ICAM-1 protein and mRNA expression in the lung tissue of heroin-treated groups were significantly increased as compared with that of the control group (P<0.01), and correlated with the ALI parameters in a time-dependent manner. Increasing of ICAM-1 expression was involved in the formation of heroin-induced lung injury. Furthermore, the level of expression was positively correlated with the severity of lung injury. 展开更多
关键词 HEROIN acute lung injury intercellular adhesion molecule-1
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Pingyangmycin-regulated Expressions of Adhesion Molecules in Human Venous Malformation Endothelial Cells 被引量:1
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作者 贾玉林 贾俊 赵怡芳 《Journal of Huazhong University of Science and Technology(Medical Sciences)》 SCIE CAS 2012年第5期760-766,共7页
Pingyangmycin (bleomycin A5 hydrochloride,PYM) is one of the anti-neoplastic agents which have been commonly used to treat venous malformations.However,the underlying mechanism by which PYM treats venous malformations... Pingyangmycin (bleomycin A5 hydrochloride,PYM) is one of the anti-neoplastic agents which have been commonly used to treat venous malformations.However,the underlying mechanism by which PYM treats venous malformations remains poorly understood.It was reported that venous endothelial cells could recruit neutrophils via adhesion molecules (E-selectin,ICAM-1,ICAM-3,VCAM-1) during the acute/chronic inflammation and subsequent histological fibrosis after sclerotherapy with PYM.This study explored if the expression of E-selectin,ICAM-1,ICAM-3 and VCAM-1 in human venous malformation endothelial cells could be affected by PYM.HVMECs were cultured from human venous malformation tissue.Expressions of E-selectin,ICAM-1,ICAM-3 and VCAM-1 on HVMECs in response to PYM were analyzed by cell ELISA.The relative levels of mRNA expression in the cells were semi-quantified.The results showed that PYM up-regulated the expressions of E-selectin,ICAM-3,VCAM-1 and ICAM-1 in both time-and concentration-dependent manner.Our findings suggested that PYM could induce the expression of adhesion molecules in HVMECs,which might be a possible mechanism by which sclerotherapy by intralesional injection of PYM treats venous malformations. 展开更多
关键词 E-SELECTIN intercellular adhesion molecule-1 intercellular adhesion molecule-3 vascular cell adhesion molecule-1 cell culture
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Ubiquitin Reduces Expression of Intercellular Adhesion Molecules and Tumor Necrosis Factor-α in Lung Tissue of Experimental Acute Lung Injury
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作者 Zhenyang He Xing Juan 《World Journal of Vaccines》 2012年第3期151-154,共4页
Background Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) are two important cytokines in inflammatory response, which may induce rolling and adhesion of both leukocytes and l... Background Intercellular adhesion molecule-1 (ICAM-1) and vascular cell adhesion molecule-1 (VCAM-1) are two important cytokines in inflammatory response, which may induce rolling and adhesion of both leukocytes and lymphocytes, while modulating vascular permeability at the same time. These adhesion molecules usually serve as surrogate markers of activation and injury of vascular endothelial cells. Tumor necrosis factor-α (TNF-α) is a key factor to induce the expression and production of the above cell adhesion molecules. However, it remains to be elucidated whether exogenous ubiquitin exerts any effect on the cytokines in sepsis-induced ALI. Methods Sixty mice were devided randomly into five groups with twelve mice in each group, i.e. CLP group, SHAM group, UB1 group (10 mg/kg), UB2 group (5 mg/kg) and UB3 group(1 mg/kg). Mice of SHAM group underwent sham operation, and other four groups underwent CLP. Six hours after surgery, mice of three UB groups received ubiquitin by caudal vein injection while CLP and SHAM group received vehicle. Seven hours after surgery, blood and lungs of all mice were collected. ICAM-1, VCAM-1 and TNF-α level of 9% lung homogenate and serum TNF-α level were measured by ELISA. Results Pulmonary ICAM-1, VCAM-1 and TNF-α level of three UB groups were lower than CLP and SHAM group, and there were several comparisons with a statistically significant difference. Serum TNF-α level of three UB groups were slightly lower than CLP group, but far higher than SHAM group. Pulmonary ICAM-1 level, VCAM-1 level and serum TNF-α level of UB3 group were lower than UB1 and UB2 group, and there was a significant difference in VCAM-1 between UB3 and UB1 group. Pulmonary TNF-α level of UB3 group was slightly higher than UB1 and UB2 group. 展开更多
关键词 UBIQUITIN Acute Lung Injury INTERCELLULAR CELL adhesion molecule-1 Vascular CELL adhesion molecule-1 Tumor Necrosis Factor-α
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自身免疫性肝炎和原发性胆汁性肝硬化患者检测可溶性细胞间黏附分子1的临床价值 被引量:3
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作者 叶青 韩涛 李宁 《临床肝胆病杂志》 CAS 2014年第5期417-420,共4页
目的探讨原发性胆汁性肝硬化(PBC)和自身免疫性肝炎(AIH)患者中检测可溶性细胞间黏附分子1(sICAM-1)的临床价值。方法选择2012年6月-2013年9月住院及门诊就诊的PBC患者61例,其中初发患者29例,缓解期患者21例,复发患者11例;AIH患者共59例... 目的探讨原发性胆汁性肝硬化(PBC)和自身免疫性肝炎(AIH)患者中检测可溶性细胞间黏附分子1(sICAM-1)的临床价值。方法选择2012年6月-2013年9月住院及门诊就诊的PBC患者61例,其中初发患者29例,缓解期患者21例,复发患者11例;AIH患者共59例,其中初发患者26例,缓解期患者20例,复发患者13例。健康对照50例。血清sICAM-1测定方法为双抗体夹心酶联免疫吸附法,采用生物化学酶法测定血清ALT、TBil。各组间比较采用方差分析,相关性采用Pearson相关分析。结果PBC患者中,初发组和复发组sICAM-1水平均明显高于缓解组和对照组(P均=0.000);初发组和复发组之间sICAM-1水平差异无统计学意义(P=0.484);缓解组sICAM-1水平高于对照组(P=0.000);AIH患者中,初发组和复发组sICAM-1水平均明显高于缓解组和对照组(P均=0.000);初发组和复发组之间sICAM-1水平差异无统计学意义(P=0.802);缓解组和对照组之间sICAM-1水平差异无统计学意义(P=0.281);PBC、AIH患者血清sICAM-1与ALT呈正相关(r=0.664,P=0.000;r=0.784,P=0.000);PBC、AIH患者血清sICAM-1与TBil呈正相关(r=0.715,P=0.000;r=0.580,P=0.000)。结论 sICAM-1可能参与PBC、AIH的免疫损伤机制,PBC、AIH患者血清sICAM-1水平升高程度与肝损害严重程度有密切关系,临床上动态观察其血清水平的变化,可望在病情活动评估、判断预后、指导治疗中起重要作用。 展开更多
关键词 胞间粘附分子1 肝硬化 胆汁性 肝炎 自身免疫性 INTERCELLULAR adhesion molecule-1
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微波辐射对大鼠睾丸闭锁蛋白和连接黏附分子-1表达的影响 被引量:6
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作者 高晓芳 王水明 +5 位作者 彭瑞云 左红艳 王丽峰 高亚兵 董霁 苏镇涛 《中华男科学杂志》 CAS CSCD 北大核心 2010年第1期10-13,共4页
目的:探讨微波辐射后大鼠血睾屏障紧密连接闭锁蛋白(occludin)及连接黏附分子-1(JAM-1)表达的改变及意义。方法:80只雄性Wistar大鼠经平均功率密度为0、10、30、100mW/cm2微波辐射5min,于辐射后6h,1、3、7、14d,采用Western印迹和图像... 目的:探讨微波辐射后大鼠血睾屏障紧密连接闭锁蛋白(occludin)及连接黏附分子-1(JAM-1)表达的改变及意义。方法:80只雄性Wistar大鼠经平均功率密度为0、10、30、100mW/cm2微波辐射5min,于辐射后6h,1、3、7、14d,采用Western印迹和图像分析技术检测闭锁蛋白和JAM-1蛋白表达的动态变化。结果:10、30和100mW/cm2微波辐射后分别在3~7d、6h~7d和6h~14d闭锁蛋白表达明显下调(P<0.05),而JAM-1蛋白分别于辐射后3~7d、1~7d和1~14d表达亦明显下调(P<0.05)。结论:闭锁蛋白及JAM-1蛋白表达减少可能在微波辐射致血睾屏障损伤中发挥重要作用。 展开更多
关键词 微波辐射 血睾屏障 闭锁蛋白 连接黏附分子-1 大鼠
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双歧杆菌三联活菌对DSS诱导结肠炎鼠肠紧密连接蛋白JAM-1的影响 被引量:5
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作者 张迎娣 余婷婷 +3 位作者 朱云娟 赵小静 程秀琴 张红杰 《世界华人消化杂志》 CAS 2016年第2期187-195,共9页
目的:探讨双歧杆菌三联活菌对葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导的结肠炎小鼠肠道炎症及紧密连接蛋白连接黏附分子1(junctional adhesion molecule-1,JAM-1)表达与分布的影响.方法:将SPF级♀Balb/c小鼠(8-10周龄)随机分为5... 目的:探讨双歧杆菌三联活菌对葡聚糖硫酸钠(dextran sulfate sodium,DSS)诱导的结肠炎小鼠肠道炎症及紧密连接蛋白连接黏附分子1(junctional adhesion molecule-1,JAM-1)表达与分布的影响.方法:将SPF级♀Balb/c小鼠(8-10周龄)随机分为5组:正常对照NC组、益生菌BB组(正常小鼠予益生菌灌胃14 d)、急性DSS模型组(生理盐水灌胃14 d+第8天起4%DSS溶液自由饮用7 d)、BD组(益生菌灌胃7 d+第8天起4%D S S溶液自由饮用7 d)、B D B组(益生菌灌胃14 d+第8天起4%D S S溶液自由饮用7 d).记录小鼠每日体质量变化.第15天处死小鼠、分离结肠,观察各组肠道损伤情况,以组织病理学积分评估结肠炎的严重程度.采用Western blot法、免疫组织化学法(IHC)检测结肠组织中JAM-1的表达及分布.结果:与D S S模型组相比,益生菌干预组(BD、BDB组)小鼠减轻的体质量得到不同程度恢复(95.17%±3.34%、87.17%±1.83%vs 81.49%±2.16%,P1<0.01,P2=0.08),病变结肠缩短程度、肠黏膜水肿,上皮细胞破坏程度及肠道炎症细胞浸润减轻,且BD组小鼠的改善程度优于BDB组.Western blot结果显示给予益生菌干预,BD、B D B组肠黏膜组织JAM-1蛋白的表达水平较DSS组显著增加(0.725±0.027、0.739±0.033 vs 0.454±0.073,均P<0.05).免疫组织化学结果显示DSS模型组小鼠结肠黏膜JAM-1染色强度明显减弱,染色细胞数减少,分布散乱、中断,益生菌处理后JAM-1染色强度及阳性细胞数减少程度显著改善.仅给予益生菌灌胃的小鼠,体质量、结肠大体、镜下表现以及结肠组织中JAM-1蛋白表达水平及分布,与正常小鼠无显著差异(P>0.05).结论:双歧杆菌三联活菌可能通过影响JAM-1蛋白的表达与分布,改善肠黏膜的屏障功能,从而减轻小鼠急性期肠道炎症反应. 展开更多
关键词 双歧杆菌三联活菌 葡聚糖硫酸钠 结肠炎 紧密连接蛋白 连接黏附分子1
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鼻息肉组织半乳凝素3和胞间黏附分子1表达在嗜酸细胞浸润中的意义 被引量:2
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作者 孔红 刘巍巍 +2 位作者 郑军 高鸽 张旭鹤 《中国耳鼻咽喉头颈外科》 北大核心 2010年第7期379-380,共2页
鼻息肉的发病机制至今尚不清楚,近年研究结果表明,鼻息肉是鼻黏膜的慢性、持续性嗜酸细胞(eosinophil,Eos)性炎症。Bachert等曾指出揭示局部大量Eos浸润机制是了解鼻息肉本质的首要问题。国内、外一些研究,包括我们以前的报道证明鼻... 鼻息肉的发病机制至今尚不清楚,近年研究结果表明,鼻息肉是鼻黏膜的慢性、持续性嗜酸细胞(eosinophil,Eos)性炎症。Bachert等曾指出揭示局部大量Eos浸润机制是了解鼻息肉本质的首要问题。国内、外一些研究,包括我们以前的报道证明鼻息肉组织中Eos大量聚集, 展开更多
关键词 鼻息肉(Nanal Polyps) 免疫组织化学(Immunohistochemistry) 半乳糖凝集素3(Galectin 3) 胞间黏附分子1(Intercellular adhesion molecule-1) 嗜酸细胞(Eosinophils)
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鼻息肉中细胞间黏附分子1和E-选择素的表达及意义 被引量:1
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作者 刘大英 张燕 +2 位作者 李胜水 李双标 许华 《中国耳鼻咽喉头颈外科》 北大核心 2011年第1期43-44,共2页
鼻息肉是耳鼻咽喉科常见病,严重危害人们身心健康。鼻息肉发病机制至今仍未完全阐明,众多研究表明嗜酸性粒细胞(eosinophils,Eos)浸润是鼻息肉最显著的病理特征。因此,探讨以Eos为代表的炎性细胞聚集和活化成为研究鼻息肉发病机制的... 鼻息肉是耳鼻咽喉科常见病,严重危害人们身心健康。鼻息肉发病机制至今仍未完全阐明,众多研究表明嗜酸性粒细胞(eosinophils,Eos)浸润是鼻息肉最显著的病理特征。因此,探讨以Eos为代表的炎性细胞聚集和活化成为研究鼻息肉发病机制的热点。本文拟通过分析鼻息肉组织中细胞间黏附分子1(intercellular adhesion molecule-1,ICAM.1)、E-选择素的表达,探讨其与Eos浸润及鼻息肉形成的关系。 展开更多
关键词 鼻息肉(Nasal Polyps) 胞间黏附分子1(Intercellular adhesion molecule-1) E选择素(E-Selectin) 细胞黏附分子(Cell adhesion Molecules) 免疫组织化学(Immunohistochemistry)
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连接粘附分子-1在人角膜上皮中的表达(英文)
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作者 陈立忠 洪晶 +1 位作者 海老原伸行 村上晶 《国际眼科杂志》 CAS 2007年第2期298-300,共3页
目的:检测连接粘附分子-1(junction adhesion molecule,JAM-1)在正常人角膜上皮各层中的表达及分布特点并与咬合蛋白(occludin)进行比较。方法:培养人角膜上皮细胞提取细胞总RNA。以逆转录后获得的cDNA为模板PCR扩增目的基因JAM-1及occl... 目的:检测连接粘附分子-1(junction adhesion molecule,JAM-1)在正常人角膜上皮各层中的表达及分布特点并与咬合蛋白(occludin)进行比较。方法:培养人角膜上皮细胞提取细胞总RNA。以逆转录后获得的cDNA为模板PCR扩增目的基因JAM-1及occludin。流式细胞仪检测JAM-1蛋白表达。双重免疫荧光观察JAM-1与occludin在正常人角膜上皮组织的原位表达。结果:通过RT-PCR在培养人角膜上皮细胞中检测到JAM-1与occludin扩增片段;流式细胞仪检测到JAM-1蛋白表达;双重免疫荧光结果显示occludin染色主要位于表层上皮层细胞之间;而JAM-1荧光染色不仅见于上皮表层,在整个上皮层细胞之间均可见其荧光反应。结论:Occludin主要位于正常人角膜上皮表层细胞之间,JAM-1在正常人角膜上皮的全层中均有表达。 展开更多
关键词 连接粘附分子-1 检测 咬合蛋白 角膜上皮细胞
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