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Application of the Just-in-Time Teaching(JiTT)Model in Bilingual Courses of Chinese-Foreign Cooperative Education 被引量:1
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作者 Na Yao Qingwen Yuan Ping Yu 《Journal of Contemporary Educational Research》 2023年第12期86-91,共6页
The teaching abilities of bilingual teachers have emerged as one of the major bottlenecks hindering the further development of Chinese-foreign cooperative education projects.Implementing the JiTT(Just-in-Time Teaching... The teaching abilities of bilingual teachers have emerged as one of the major bottlenecks hindering the further development of Chinese-foreign cooperative education projects.Implementing the JiTT(Just-in-Time Teaching)model in bilingual courses of Chinese-foreign cooperative education can effectively integrate information technology with traditional classroom teaching.This integration enhances teaching quality and effectiveness,encourages university instructors to improve their diverse and collective capabilities,promotes digital development,and contributes to the enhancement of students’learning abilities and overall improvement. 展开更多
关键词 just-in-time Teaching model Information technology BILINGUAL
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Enhancing the Quality of Low-Light Printed Circuit Board Images through Hue, Saturation, and Value Channel Processing and Improved Multi-Scale Retinex
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作者 Huichao Shang Penglei Li Xiangqian Peng 《Journal of Computer and Communications》 2024年第1期1-10,共10页
To address the issue of deteriorated PCB image quality in the quality inspection process due to insufficient or uneven lighting, we proposed an image enhancement fusion algorithm based on different color spaces. First... To address the issue of deteriorated PCB image quality in the quality inspection process due to insufficient or uneven lighting, we proposed an image enhancement fusion algorithm based on different color spaces. Firstly, an improved MSRCR method was employed for brightness enhancement of the original image. Next, the color space of the original image was transformed from RGB to HSV, followed by processing the S-channel image using bilateral filtering and contrast stretching algorithms. The V-channel image was subjected to brightness enhancement using adaptive Gamma and CLAHE algorithms. Subsequently, the processed image was transformed back to the RGB color space from HSV. Finally, the images processed by the two algorithms were fused to create a new RGB image, and color restoration was performed on the fused image. Comparative experiments with other methods indicated that the contrast of the image was optimized, texture features were more abundantly preserved, brightness levels were significantly improved, and color distortion was prevented effectively, thus enhancing the quality of low-lit PCB images. 展开更多
关键词 Low-lit PCB Images Spatial Transformation Image Enhancement Image Fusion HSV
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基于互信息和Just-in-Time优化的回声状态网络 被引量:7
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作者 张衡 王河山 《郑州大学学报(工学版)》 CAS 北大核心 2017年第5期1-6,共6页
为了提高回声状态网络(ESN)的适应性,提出基于互信息(MI)和Just-in-Time(JIT)的优化方法,对ESN的输入伸缩参数以及输出层进行优化,所得网络称为MI-JIT-ESN.ESN的优化方法分为两部分:一是基于网络输入与输出之间的互信息,对网络的多个输... 为了提高回声状态网络(ESN)的适应性,提出基于互信息(MI)和Just-in-Time(JIT)的优化方法,对ESN的输入伸缩参数以及输出层进行优化,所得网络称为MI-JIT-ESN.ESN的优化方法分为两部分:一是基于网络输入与输出之间的互信息,对网络的多个输入伸缩参数进行调整;二是基于JIT优化的局部输出层,对ESN的隐层输出数据进行局部重新建模,从而提升ESN输出层的回归拟合精度.将MI-JIT-ESN应用于青霉素补料分批发酵过程建模.结果显示,MI-JIT优化方法能提高模型的适应性,并优于其他比较方法. 展开更多
关键词 回声状态网络 互信息 just-in-time 优化 建模 青霉素发酵
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Calibration of soft sensor by using Just-in-time modeling and Ada Boost learning method 被引量:11
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作者 Huan Min Xionglin Luo 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2016年第8期1038-1046,共9页
Soft sensor is an efficacious solution to predict the hard-to-measure target variable by using the process variables.In practical application scenarios, however, the feedback cycle of target variable is usually larger... Soft sensor is an efficacious solution to predict the hard-to-measure target variable by using the process variables.In practical application scenarios, however, the feedback cycle of target variable is usually larger than that of the process variables, which causes the deficiency of prediction errors. Consequently soft sensor cannot be calibrated timely and deteriorates. We proposed a soft sensor calibration method by using Just-in-time modeling and Ada Boost learning method. A moving window consisting of a primary part and a secondary part is constructed.The primary part is made of history data from certain number of constant feedback cycles of target variable and the secondary part includes some coarse target values estimated initially by Just-in-time modeling during the latest feedback cycle of target variable. The data set of the whole moving window is processed by Ada Boost learning method to build an auxiliary estimation model and then target variable values of the latest corresponding feedback cycle are reestimated. Finally the soft sensor model is calibrated by using the reestimated target variable values when the target feedback is unavailable; otherwise using the feedback value. The feasibility and effectiveness of the proposed calibration method is tested and verified through a series of comparative experiments on a pH neutralization facility in our laboratory. 展开更多
关键词 Process control Measurement Soft sensor CALIBRATION DETERIORATION Moving WINDOW just-in-time ADA BOOST
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Just-in-time learning based integrated MPC-ILC control for batch processes 被引量:4
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作者 Li Jia Wendan Tan 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2018年第8期1713-1720,共8页
Considering the two-dimension(2 D) characteristic and the unknown optimal trajectory problem of the batch processes, an integrated model predictive control-iterative learning control(MPC-ILC) for batch processes is pr... Considering the two-dimension(2 D) characteristic and the unknown optimal trajectory problem of the batch processes, an integrated model predictive control-iterative learning control(MPC-ILC) for batch processes is proposed in this paper. Firstly, the batch-axis information and time-axis information are combined into one quadratic performance index. It implies the integration of ILC and MPC algorithm idea, which leads to superior tracking performance and better robustness against disturbance and uncertainty. To address the problem of the unknown optimal trajectory, both time-varying prediction horizon and end product quality control are employed. Moreover, an integrated 2 D just-in-time learning(JITL) model is used to improve the predictive accuracy. Furthermore, rigorous description and proof are presented to prove the convergence and tracking performance of the proposed MPC-ILC strategy. The simulation results show the effectiveness of the proposed method. 展开更多
关键词 Model predictive control Batch process just-in-time learning (JITL) model
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A New Efficient Obstacle Avoidance Control Method for Cars Based on Big Data and Just-in-Time Modeling 被引量:1
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作者 Tatsuya Kai 《Journal of Computer and Communications》 2018年第11期12-22,共11页
This paper provides a new obstacle avoidance control method for cars based on big data and just-in-time modeling. Just-in-time modeling is a new kind of data-driven control technique in the age of big data and is used... This paper provides a new obstacle avoidance control method for cars based on big data and just-in-time modeling. Just-in-time modeling is a new kind of data-driven control technique in the age of big data and is used in various real systems. The main property of the proposed method is that a gain and a control time which are parameters in the control input to avoid an encountered obstacle are computed from a database which includes a lot of driving data in various situations. Especially, the important advantage of the method is small computation time, and hence it realizes real-time obstacle avoidance control for cars. From some numerical simulations, it is showed that the new control method can make the car avoid various obstacles efficiently in comparison with the previous method. 展开更多
关键词 Big Data just-in-time MODELING CARS OBSTACLE AVOIDANCE Control
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Minimizing Distance Using Just-in-Time Teaching and Flipped Classroom 被引量:1
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作者 Mahmuda Akhter 《Sino-US English Teaching》 2015年第1期20-25,共6页
JiTT (Just-in-Time Teaching) and flipped classroom are two innovations in academia where high-tech and no-tech resources are blended to encourage more interaction and creativity among students. These two methods als... JiTT (Just-in-Time Teaching) and flipped classroom are two innovations in academia where high-tech and no-tech resources are blended to encourage more interaction and creativity among students. These two methods also help minimize the dependency on face-to-face lecture and make the class time more creative and effective. This study is the very first attempt of implementing these strategies in Bangladesh. It explores the prospect of JiTT and flipped classroom at tertiary level in Bangladesh. In the first part of this paper, recent studies on JiTT and flipped classroom are discussed to shed light on their effectiveness and problems. The latter part explains the data and research findings. Before collecting the data, JiTT and flipped classroom were used with 33 students of undergraduate level who were students of"ELT Methodology" course. After the implementation of the methods, a questionnaire survey was conducted to collect data regarding their response to these two methods. The study reveals that JiTT and flipped classroom help cover more topics in a short time while enhancing students' interaction and participation. They make students come to the class prepared and reflect on their learning. Teachers can understand students need better and provide required feedback. The overall interaction among students and teachers can be improved using JiTT and flipped classroom since everyone is clear about each other's existing knowledge and learning objectives. 展开更多
关键词 just-in-time technology teaching strategies flipped classroom video lecture
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Supervised local and non-local structure preserving projections with application to just-in-time learning for adaptive soft sensor 被引量:4
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作者 邵伟明 田学民 王平 《Chinese Journal of Chemical Engineering》 SCIE EI CAS CSCD 2015年第12期1925-1934,共10页
In soft sensor field, just-in-time learning(JITL) is an effective approach to model nonlinear and time varying processes. However, most similarity criterions in JITL are computed in the input space only while ignoring... In soft sensor field, just-in-time learning(JITL) is an effective approach to model nonlinear and time varying processes. However, most similarity criterions in JITL are computed in the input space only while ignoring important output information, which may lead to inaccurate construction of relevant sample set. To solve this problem, we propose a novel supervised feature extraction method suitable for the regression problem called supervised local and non-local structure preserving projections(SLNSPP), in which both input and output information can be easily and effectively incorporated through a newly defined similarity index. The SLNSPP can not only retain the virtue of locality preserving projections but also prevent faraway points from nearing after projection,which endues SLNSPP with powerful discriminating ability. Such two good properties of SLNSPP are desirable for JITL as they are expected to enhance the accuracy of similar sample selection. Consequently, we present a SLNSPP-JITL framework for developing adaptive soft sensor, including a sparse learning strategy to limit the scale and update the frequency of database. Finally, two case studies are conducted with benchmark datasets to evaluate the performance of the proposed schemes. The results demonstrate the effectiveness of LNSPP and SLNSPP. 展开更多
关键词 Adaptive soft sensor just-in-time learning Supervised local and non-local structure preserving projections Locality preserving projections Database monitoring
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Online Batch Process Monitoring Based on Just-in-Time Learning and Independent Component Analysis 被引量:1
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作者 WANG Li SHI Hong-bo 《Journal of Donghua University(English Edition)》 EI CAS 2016年第6期944-948,共5页
A new method was developed for batch process monitoring in this paper. In the devdopad method, just-in-time learning ( JITL ) and independent component analysis (ICA) were integrated to build JITL-ICA monitoring s... A new method was developed for batch process monitoring in this paper. In the devdopad method, just-in-time learning ( JITL ) and independent component analysis (ICA) were integrated to build JITL-ICA monitoring scheme. JITL was employed to tackle with the characteristics of batch process such as inherent time- varying dynamics, multiple operating phases, and especially the case of uneven length stage. According to new coming test data, the most correlated segmentation was obtained from batch-wise unfolded training data by JITL. Then, ICA served as the principal components extraction approach. Therefore, the non.Gaussian distributed data can also be addressed under this modeling framework. The effectiveness and superiority of JITL-ICA based monitoring method was demonstrated by fed-batch penicillin fermentation. 展开更多
关键词 batch process monitoring just-in-time learning(JITL) independent component analysis(ICA)
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A Preliminary Study of Just-in-Time Methods for a Seamless Public Transportation Information Framework
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作者 Spring C. Hsu 《Intelligent Information Management》 2012年第1期1-5,共5页
A seamless multimodal transit system has been a goal for transportation planners and users. Owing to the developments of advanced public transportation and telecom technologies such as automatic vehicle location (AVL)... A seamless multimodal transit system has been a goal for transportation planners and users. Owing to the developments of advanced public transportation and telecom technologies such as automatic vehicle location (AVL) and real-time passenger information system, key just-in-time (JIT) concepts can now be realized in process design and coordinated scheduling to shape a seamless multimodal transit system. JIT refers to a production system that times both movements of goods during production and delivery from suppliers together. It meets the same logic for seamless multimodal services. Therefore, this study innovatively analyzes possible public transportation technologies for JIT uses;moreover, to layout the possible application frame-work of a transportation technology based JIT multimodal transit system for main station area in Taipei city. With this innovative framework, multimodal transit services can deliver to the right place at right time. 展开更多
关键词 just-in-time (JIT) KANBAN Management Mixed-Model Production PUBLIC TRANSPORTATION SEAMLESS Services
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Just-In-Time Hypermedia
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作者 Zong Chen Li Zhang 《Journal of Software Engineering and Applications》 2013年第5期32-36,共5页
Many analytical or computational applications create documents and display screens in response to user queries “dynamically” or in “real time”. Hypermedia features must be generated “just in time” – automatical... Many analytical or computational applications create documents and display screens in response to user queries “dynamically” or in “real time”. Hypermedia features must be generated “just in time” – automatically and dynamically. Conversely, the hypermedia features may have to cause target documents to be generated or re-generated. This paper presents a just-in-time hypermedia framework to provide hypermedia support of virtual documents. 展开更多
关键词 just-in-time HYPERMEDIA VIRTUAL DOCUMENT
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基于“Just-in-Time”原理的信息系统的综合发展
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作者 李斌 《管理观察》 1999年第1期42-43,共2页
研究了提高解决商业问题的信息技术设备利用率的问题。研究了解决上述问题的方法:改进信息系统;提高信息技术设备培训的强度;改善实用问题解决过程的文件编制;改进信息技术设备的应用规划;在准时制(Just-in-TimeSystem)的组织... 研究了提高解决商业问题的信息技术设备利用率的问题。研究了解决上述问题的方法:改进信息系统;提高信息技术设备培训的强度;改善实用问题解决过程的文件编制;改进信息技术设备的应用规划;在准时制(Just-in-TimeSystem)的组织原理基础上,改善信息技术设备应用过程的管理和标准化。还研究了下列问题:提高信息技术设备对用户需求的满足程度;信息技术设备应用过程的结构化;提高计算机应用对管理质量影响的客观性;研究信息技术设备的合理基础设施。较大注意力集中在最有效的信息技术设备的基础上和应用服务器的最佳状态下,保证信息系统灵活的发展。对在现有技术和财政限制情况下,发展信息技术设备的分阶段的规划方法进行了说明。强调指出了,综合发展信息技术设备基本元件(计算技术设备、保证设备及其基础设施)的重要性。 展开更多
关键词 just-in-time 信息系统 信息技术设备 应用服务器 原理 计算机应用 综合发展 问题解决过程 应用过程 基础设施
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不明原因复发性流产淋巴细胞免疫治疗前后HLA抗体的变化和妊娠结局
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作者 李莉 王海燕 +2 位作者 乔杰 李蓉 刘平 《中国生育健康杂志》 2024年第6期556-560,共5页
目的研究不明原因复发性流产(RPL)患者和正常生育人群人类白细胞抗原(HLA)抗体的状态;检测淋巴细胞免疫治疗(LIT)后HLA抗体的转阳率;对比不明原因RPL患者淋巴细胞免疫治疗后HLA抗体与妊娠结局,评估HLA抗体是否可作为LIT预测妊娠结局的... 目的研究不明原因复发性流产(RPL)患者和正常生育人群人类白细胞抗原(HLA)抗体的状态;检测淋巴细胞免疫治疗(LIT)后HLA抗体的转阳率;对比不明原因RPL患者淋巴细胞免疫治疗后HLA抗体与妊娠结局,评估HLA抗体是否可作为LIT预测妊娠结局的有效指标。方法选取2014年12月3日至2015年6月10日在北京大学第三医院生殖中心门诊就诊的不明原因RPL患者24例作为RPL组,同期于北京大学第三医院体检中心体检的健康未孕女性30例作为对照组,两组年龄均在20~40岁。应用酶联免疫吸附法和流式细胞仪-微珠法,分别测定不明原因RPL患者LIT前后和健康生育者外周血的HLA特异性IgG抗体,根据HLA抗体状态分为4类,包括HLA-I类阳性、HLA-II类阳性、双阳性(HLA-Ⅰ类、Ⅱ类均阳性)和双阴性(HLA-Ⅰ类、Ⅱ类均阴性),比较两组人群HLA抗体状态,分析LIT后RPL患者HLA抗体的转阳率,及HLA抗体状态与妊娠结局的关系。结果RPL患者HLA抗体状态与健康生育者比较,差异无统计学意义;LIT促进HLA抗体转阳,LIT后不同HLA状态的活产率比较,差异无统计学意义。结论不明原因PRL患者,LIT前HLA特异性IgG抗体与正常生育者差异无统计学意义,LIT可以促进HLA抗体转阳,免疫治疗后PRL患者HLA特异性IgG抗体与妊娠结局无关,HLA特异性IgG抗体不能作为LIT预测妊娠结局的唯一参考指标,尚需要结合其他免疫指标综合分析。 展开更多
关键词 不明原因复发性流产(RPL) 淋巴细胞免疫治疗(lit) HLA抗体 封闭抗体
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康熙朝太常祭祀乐制建设中乐律的制度化——以《律吕正义》的生成为中心
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作者 温显贵 邱婧 《音乐艺术(上海音乐学院学报)》 北大核心 2024年第1期60-75,共16页
清王朝建立以后,乐制建设在艰难中缓慢转型。康熙前期,太常祭祀乐制有所创制,如定太常寺乐舞生、定乐器,以及在理学阴阳思想观照下提出对乐律的再讨论。康熙后期,重考乐律,将理学阴阳思想作为康熙十四律的理论支撑,依据精密计算,用字谱... 清王朝建立以后,乐制建设在艰难中缓慢转型。康熙前期,太常祭祀乐制有所创制,如定太常寺乐舞生、定乐器,以及在理学阴阳思想观照下提出对乐律的再讨论。康熙后期,重考乐律,将理学阴阳思想作为康熙十四律的理论支撑,依据精密计算,用字谱、旋宫体现其实践性。《律吕正义》成书以后,康熙迅速将新律用于太常祭祀,并亲定全部雅乐。因此《律吕正义》的诞生标志着帝王意志对乐律的重塑,体现出帝王治世与文化思想的相互呼应,也是“乐律”这一科学性概念的制度化体现。 展开更多
关键词 太常祭祀 乐制 《律吕正义》 康熙
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LIT对小鼠抗弓形虫感染的保护作用 被引量:4
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作者 林爱芬 陆绍红 +4 位作者 陈彩华 李思温 陈睿 陆漩辉 黄富泉 《中国寄生虫学与寄生虫病杂志》 CAS CSCD 北大核心 1999年第6期387-389,共3页
目的: 探讨激光照射弓形虫速殖子(LIT) 的免疫保护力及其诱导的免疫应答水平。方法: 应用4.5 WLIT免疫ICR小鼠,并用活体弓形虫速殖子攻击感染,观察LIT抗虫效果。分别用间接免疫荧光法和ELISA法测定T淋巴细... 目的: 探讨激光照射弓形虫速殖子(LIT) 的免疫保护力及其诱导的免疫应答水平。方法: 应用4.5 WLIT免疫ICR小鼠,并用活体弓形虫速殖子攻击感染,观察LIT抗虫效果。分别用间接免疫荧光法和ELISA法测定T淋巴细胞CD4+ /CD8+ 亚群及特异性IgG抗体。结果: LIT免疫的小鼠具有抗急性弓形虫感染的能力, 能延长小鼠生存时间, 并能导致机体CD4+ 、CD4+ /CD8+ 显著升高及特异性IgG抗体产生。结论: 4.5 W LIT经腹腔免疫接种小鼠, 能诱导部分的保护性免疫力, 展开更多
关键词 激光照射 弓形虫速殖子 免疫应答 lit
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前葡萄膜炎中医证素特点研究
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作者 毕徐齐 朱笑莹 杨永升 《中国中医眼科杂志》 2024年第8期742-747,共6页
目的研究与总结前葡萄膜炎(AU)中医证素特点,为AU的规范临床辨证论治提供参考。方法收集中国知网、万方、维普数据库从建库至2023年3月31日正式发表的关于AU中医证型的相关文献,对中医证名进行规范化处理以及证型的拆解,将文献中筛选出... 目的研究与总结前葡萄膜炎(AU)中医证素特点,为AU的规范临床辨证论治提供参考。方法收集中国知网、万方、维普数据库从建库至2023年3月31日正式发表的关于AU中医证型的相关文献,对中医证名进行规范化处理以及证型的拆解,将文献中筛选出的证型条目及病例数归纳建立Excel软件数据库。对病位证素及病性证素进行频数、频率描述分析,对证素进行系统聚类分析和关联规则分析。结果(1)一般情况:纳入文献24篇,总病例数1,685例。(2)证型及证素分布:频率排名前5位的证型有肝经风热证(329次,19.53%)、肝胆火炽证(224次,13.29%)、肝火炽盛证(205次,12.17%)、阴虚火旺证(166次,9.85%)、肝胆湿热证(94次,5.58%)。(3)证素特点:病位证素有5个,以频率从高到低排名依次是肝(1,035次,61.42%)、胆(335次,19.88%)、表(163次,9.64%)、肾(117次,6.94%)、脾(55次,3.26%)。病性证素类型共13种,热病性出现频次最高,为1,447次(85.88%)。以虚性、实性进行分类分析,实性证素共出现了2,458次(76.42%),虚性证素共出现了758次(23.56%);其中,热证素在实性证素中出现频次最高,共1,447次(44.99%);阴虚证素在虚性证素中出现频次最高,共374次(11.63%)。(4)证素关联规则分析:共得到8条强关联规则,其中二项强关联规则5个,三项强关联规则3个,推断出5个基础证候,分别为肝火炽盛证、风热证、湿热证、肝胆火炽证、肝经风热证。关联规则网状图显示肝与热的关联最强,推断基础证候为肝火炽盛。结论肝经风热、肝胆火炽、肝火炽盛3个证型是前葡萄膜炎患者的主要证型。前葡萄膜炎的重要病位证素为肝,重要病性证素为热,病性特点以实证为主。 展开更多
关键词 前葡萄膜炎 证素 辨证论治 文献研究
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LiH和LiT与H_2O反应机理研究
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作者 苟振志 何彬 +1 位作者 罗忠辉 喻凤梅 《原子能科学技术》 EI CAS CSCD 北大核心 2014年第12期2195-2199,共5页
氢材料在微量H2O、CO2、O2和N2存在下可能发生物理化学反应,使材料的物理品位下降。由于反应过程十分复杂,很难从实验上准确获取这类反应的最佳通道和具体产物信息,因此,从理论上研究氢材料分子的物理化学性质及其化学反应机制,了解化... 氢材料在微量H2O、CO2、O2和N2存在下可能发生物理化学反应,使材料的物理品位下降。由于反应过程十分复杂,很难从实验上准确获取这类反应的最佳通道和具体产物信息,因此,从理论上研究氢材料分子的物理化学性质及其化学反应机制,了解化学反应过程具有十分重要的意义。本文使用Gaussian03软件包和Gaussview工具软件,在6-311G(d)全电子基函数水平上,应用二阶微扰理论优化得到了6 LiH、6 LiT与H2O反应的中间体、过渡态及产物的结构,总能量,振动频率和零点能等。通过计算发现6LiH、6LiT均只有1个反应通道,6LiH与H2O反应的焓变、活化能和反应速率常数分别为-156.99kJ/mol、8.95kJ/mol和3.75×1010(mol·dm-3)-1/s,6 LiT与H2O反应的焓变、活化能和反应速率常数分别为-159.02kJ/mol、9.92kJ/mol和1.72×1010(mol·dm-3)-1/s。 展开更多
关键词 LIH lit H2O 反应机理
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LC-LIT-MS法同时检测硬膜外麻醉致死犬体内利多卡因及其代谢物MEGX和GX的研究
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作者 张潮 尉志文 +2 位作者 王锦凯 牛卫芬 贠克明 《山西医科大学学报》 CAS 2021年第8期1064-1068,共5页
目的建立同时检测硬膜外麻醉致死犬血液和肝脏中利多卡因及其代谢物单乙基甘氨酰二甲苯胺(MEGX)和甘氨二甲基苯酰胺(GX)的液相色谱-离子肼质谱(LC-LIT-MS)方法。方法犬3只经硬膜外穿刺,按利多卡因硬膜外麻醉用药最大量的5倍注射(63.35 m... 目的建立同时检测硬膜外麻醉致死犬血液和肝脏中利多卡因及其代谢物单乙基甘氨酰二甲苯胺(MEGX)和甘氨二甲基苯酰胺(GX)的液相色谱-离子肼质谱(LC-LIT-MS)方法。方法犬3只经硬膜外穿刺,按利多卡因硬膜外麻醉用药最大量的5倍注射(63.35 mg/kg),待呼吸、心跳等生命体征完全消失后解剖并且收集检材,样品采用液液萃取法前处理,采用离子肼质谱联用进行定性检测。高效液相色谱内标法建立工作曲线,空白添加法进行方法回收率考察。结果经硬膜外注射利多卡因后,犬出现嗜睡、流涎和抽搐等症状,犬于注射20 min后死亡。解剖见导管进入硬膜外腔隙,提示造模成功。液相色谱-离子肼质谱可以同时检测心血和肝脏中利多卡因、MEGX和GX。心血和肝脏中利多卡因的线性范围分别为0.1-100 ng/ml和0.1-100 ng/mg,最低检出限为0.004 ng,信噪比(S/N)=3;MEGX的线性范围分别为2-250 ng/ml和2-250 ng/mg,最低检出限为1 ng(S/N=3);GX的线性范围分别为2-250 ng/ml和2-250 ng/mg,最低检出限为1 ng(S/N=3)。回收率良好,方法稳定性好。结论本实验建立了同时检测生物检材中利多卡因及其代谢物的LC-LIT-MS检测方法,可用于对利多卡因及其代谢物的法医毒物动力学研究和利多卡因中毒案件的法医学鉴定。 展开更多
关键词 法医毒物动力学 利多卡因 单乙基甘氨酰二甲苯胺 甘氨二甲基苯酰胺 液相色谱-离子肼质谱
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影响汽车内饰光导主观均匀性判断的变量研究
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作者 沈烨东 黄光卫 +1 位作者 姚其 吴沛榆 《照明工程学报》 2024年第4期21-27,共7页
直射式光导的均匀性直接影响使用体验,为深入解析汽车内饰环境下影响光导主观均匀性判断的变量,本研究针对三项特征参数,即LED数量、亮度公差和光导中心亮度,进行了研究分析。基于图像模拟和主观评价实验,结果发现:当LED数量由40增至60... 直射式光导的均匀性直接影响使用体验,为深入解析汽车内饰环境下影响光导主观均匀性判断的变量,本研究针对三项特征参数,即LED数量、亮度公差和光导中心亮度,进行了研究分析。基于图像模拟和主观评价实验,结果发现:当LED数量由40增至60时,均匀度评分提高9.2%;当公差由0%放宽至80%时,均匀度评分降低22.6%;当中心亮度由10 cd/m 2调至2 cd/m 2时,均匀度评分降低11.5%。此外,Δu′和Δv′值与均匀度评分呈负相关。该研究揭示技术变量与用户体验的内在关联,为产品优化提供参考。 展开更多
关键词 直射式光导 汽车内饰 均匀性指标 人因照明
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非小细胞肺癌患者HSA-miR30-5p和HSA-LIT-7b表达与生存期限的关系 被引量:2
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作者 朱立欢 王志安 +1 位作者 熊汉忠 谢芳 《河北医药》 CAS 2020年第3期463-466,470,共5页
目的探讨非小细胞肺癌患者(NSCLC)HSA-miR30-5p和HSA-LIT-7b表达与其生存期限的关联。方法选择2015年1月至2018年10月,98例诊断的非小细胞肺癌患者和98例健康对照者。取静脉血样,利用miRNA定量实时PCR(qrt-PCR)技术分析受试者HSA-miR30... 目的探讨非小细胞肺癌患者(NSCLC)HSA-miR30-5p和HSA-LIT-7b表达与其生存期限的关联。方法选择2015年1月至2018年10月,98例诊断的非小细胞肺癌患者和98例健康对照者。取静脉血样,利用miRNA定量实时PCR(qrt-PCR)技术分析受试者HSA-miR30-5p和HSA-LIT-7b表达。取受试组织样本,用细胞裂解缓冲液从培养细胞中提取总蛋白,用Western blotting法和酶联免疫吸附试验定量分析检测基因表达。结果HSA-miR30-5p在非小细胞肺癌组织中的表达高于正常肺组织(P<0.01),且HSA-miR30-5p阳性表达组存活时间短于阴性表达组(P<0.05),而HSA-LIT-7b的研究结果与HSA-miR30-5p相同。基因型多态性分析显示,正常对照组SNP(G>A,rs4636297)基因型分布符合Hardy-Weinberg平衡(P=0.336),2组基因型分布差异无统计学意义(P>0.05)。结论HSA-miR30-5p和HSA-LIT-7 bmRNA的表达可能是NSCLC患者整体生存和肿瘤恶性转移行为的预测因素。 展开更多
关键词 HSA-miR30-5p HSA-lit-7b 非小细胞肺癌
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