OBJECTIVE To investigate the effects of survivin antisense oligodeoxy-nucleotid (ASODN) on proliferation and apoptosis in the chronic myeloid leukemia cell line K562. METHODS Different concentrations of an antisense o...OBJECTIVE To investigate the effects of survivin antisense oligodeoxy-nucleotid (ASODN) on proliferation and apoptosis in the chronic myeloid leukemia cell line K562. METHODS Different concentrations of an antisense oligodeoxy-nucleotid and control sequence (scrambled ODN) targeting the survivin gene were transferred into K562 by a lipofectin reagent. The MTT assay was used to measure the growth inhibitory rate, IC50, and to observe the cytotoxicity of survivin ASODN in the K562 cells. The morphologic changes in the nucleus and the apoptotic rate were observed by Hoechst33342/PI staining. Caspase-3 activity was evaluated by a kinase activity assay. The changes of survivin protein expression after transfection were detected by Western blots. RESULTS Eight hours after transfection, fluorescence in the K562 cells was well distributed. Treatment of the cells for 44 h with different concentrations of survivin ASODN produced a IC50 of 800 nmol/L. The growth inhibitory rate with 200, 400, 600 and 1000 nmol/L of survivin ASODN was 15.8±1.6%, 23.8±5.9%, 37.1±5.6% and 77.3±2.5% respectively. After 36 h of of survivin ASODN treatment, distinct morphologic changes characteristic of cell apoptosis such as karyopyknosis and conglomeration were observed by Hoechst33342/PI staining. Caspase-3 activity increased significantly after treatment of the cells with different concentrations of survivin ASODN(P<0.01)and following treatment with 800 nmol/L survivin ASODN, survivin expression decreased significantly. CONCLUSION Survivin ASODN exerts an anti-cancer effect by inducing apoptosis in K562 leukaemia cells. Up-regulated expression of caspase-3 may play a role in this process.展开更多
It is first demonstrated that dipyridamole (DP) and radiation were capable of significantly inhibiting, independently and synerglcally, clonogenlc growth in the two kinds of K562 cell lines, adriamycin (ADM) -sensitiv...It is first demonstrated that dipyridamole (DP) and radiation were capable of significantly inhibiting, independently and synerglcally, clonogenlc growth in the two kinds of K562 cell lines, adriamycin (ADM) -sensitive and ADM- resistant. DP or radiation alone Increased clonogenlc Inhibition rate (CIR) in the two kinds of cell lines in a dose- dependent fashion. DP potentiated radiosensitivity and radiation increased inhibition of DP in the two kinds of cell lines. K562/ ADM cell lines were higher sensitive to DP. radiation and combination of them than K562 cell lines (P<0. 01). There was stronger synergic inhibition of clonogenlc growth in the two kinds of cell lines when pretreated with DP than when posttreated with DP (P<0. 01).展开更多
It was first reported here that verupamil(VP) and electric beam radiation(EBR) were capable of inhibiting,independently or synergically,clonogenic growth in two kinds of K562 cell lines, adriamycin(ADM)-sensitive and ...It was first reported here that verupamil(VP) and electric beam radiation(EBR) were capable of inhibiting,independently or synergically,clonogenic growth in two kinds of K562 cell lines, adriamycin(ADM)-sensitive and ADM-resistant(K562/S and K562/ADM).Results showed that clonogenic rate(CGR) decreased by 3%-99.9% in the prasence of dependent dose-ADM(3.8μg/ml) in K562/ADM cell lines,while treated with 0.5μM-6μM of VP.VP was capable of potentiating radiosensitivity in K562/S and K562/ADM cell lines,whether before or after exposure of them to electric beam radiation,and significantly reduced CGR in these kinds of cell lines(P<0.01).展开更多
目的:结合K线(颈椎侧位X线片上C_2及C_7椎管中点的连线)分析颈椎后纵韧带骨化患者的颈椎活动度与手术预后的关系。方法:对2014年4月至2017年3月行颈椎后路单开门椎管扩大成形术的42例颈椎后纵韧带骨化(cervical ossification of the pos...目的:结合K线(颈椎侧位X线片上C_2及C_7椎管中点的连线)分析颈椎后纵韧带骨化患者的颈椎活动度与手术预后的关系。方法:对2014年4月至2017年3月行颈椎后路单开门椎管扩大成形术的42例颈椎后纵韧带骨化(cervical ossification of the posterior longitudinal ligament,OPLL)患者进行回顾性分析。根据后纵韧带骨化与K线的位置关系,患者被分为K线(+)组及K线(-)组。后纵韧带骨化病灶未超过K线称为K线(+),反之,后纵韧带骨化病灶越过K线称为K线(-)。观察术前及术后3个月JOA(Japanese Orthopedic Association)评分,并计算术后3个月JOA改善率评估患者神经功能恢复情况。通过影像学资料测量术前及术后3个月后纵韧带骨化的侵占率(occupation ratio,OOR),颈椎生理曲度(cervical lordotic angles,CLA)和颈椎前凸值(cervical lordotic value,CLV)。评估不同的K线分组中,CLV>0组和CLV≤0组之间患者的术后神经功能恢复之间的联系。结果:K线(+)组和K线(-)组患者的术前CLA分别为(14.7±9.6)°和(-6.4°±9.5)(P<0.05),术后3个月分别为(14.0±8.0)°和(-1.4±10.4)°(P<0.05);术前JOA评分分别为10.9±3.2和11.2±2.5(P>0.05),术后3个月分别为14.2±1.8和12.6±2.2(P<0.05),术后3个月JOA改善率分别为(54.7±17.6)%和(25.5±15.7)%(P<0.05)。K线(+)组中,术后3个月CLV>0组的患者29例,其术后3个月JOA改善率(52.3±17.2)%,术后3个月CLV≤0组的患者4例,其术后3个月JOA改善率为(72.2±7.8)%,术后3个月CLV≤0组患者JOA改善率明显优于术后3个月CLV>0组(P<0.05)。结论:无论患者颈椎后纵韧带骨化分类为K线(+)或K线(-),颈椎后路单开门椎管扩大成形手术都能够很好的改善患者的神经症状,尤其K线(+)组的患者,具有较好的预后效果。K线(+)组患者术后3个月CLV>0时,其JOA改善率较CLV≤0的患者差。展开更多
文摘OBJECTIVE To investigate the effects of survivin antisense oligodeoxy-nucleotid (ASODN) on proliferation and apoptosis in the chronic myeloid leukemia cell line K562. METHODS Different concentrations of an antisense oligodeoxy-nucleotid and control sequence (scrambled ODN) targeting the survivin gene were transferred into K562 by a lipofectin reagent. The MTT assay was used to measure the growth inhibitory rate, IC50, and to observe the cytotoxicity of survivin ASODN in the K562 cells. The morphologic changes in the nucleus and the apoptotic rate were observed by Hoechst33342/PI staining. Caspase-3 activity was evaluated by a kinase activity assay. The changes of survivin protein expression after transfection were detected by Western blots. RESULTS Eight hours after transfection, fluorescence in the K562 cells was well distributed. Treatment of the cells for 44 h with different concentrations of survivin ASODN produced a IC50 of 800 nmol/L. The growth inhibitory rate with 200, 400, 600 and 1000 nmol/L of survivin ASODN was 15.8±1.6%, 23.8±5.9%, 37.1±5.6% and 77.3±2.5% respectively. After 36 h of of survivin ASODN treatment, distinct morphologic changes characteristic of cell apoptosis such as karyopyknosis and conglomeration were observed by Hoechst33342/PI staining. Caspase-3 activity increased significantly after treatment of the cells with different concentrations of survivin ASODN(P<0.01)and following treatment with 800 nmol/L survivin ASODN, survivin expression decreased significantly. CONCLUSION Survivin ASODN exerts an anti-cancer effect by inducing apoptosis in K562 leukaemia cells. Up-regulated expression of caspase-3 may play a role in this process.
文摘It is first demonstrated that dipyridamole (DP) and radiation were capable of significantly inhibiting, independently and synerglcally, clonogenlc growth in the two kinds of K562 cell lines, adriamycin (ADM) -sensitive and ADM- resistant. DP or radiation alone Increased clonogenlc Inhibition rate (CIR) in the two kinds of cell lines in a dose- dependent fashion. DP potentiated radiosensitivity and radiation increased inhibition of DP in the two kinds of cell lines. K562/ ADM cell lines were higher sensitive to DP. radiation and combination of them than K562 cell lines (P<0. 01). There was stronger synergic inhibition of clonogenlc growth in the two kinds of cell lines when pretreated with DP than when posttreated with DP (P<0. 01).
文摘It was first reported here that verupamil(VP) and electric beam radiation(EBR) were capable of inhibiting,independently or synergically,clonogenic growth in two kinds of K562 cell lines, adriamycin(ADM)-sensitive and ADM-resistant(K562/S and K562/ADM).Results showed that clonogenic rate(CGR) decreased by 3%-99.9% in the prasence of dependent dose-ADM(3.8μg/ml) in K562/ADM cell lines,while treated with 0.5μM-6μM of VP.VP was capable of potentiating radiosensitivity in K562/S and K562/ADM cell lines,whether before or after exposure of them to electric beam radiation,and significantly reduced CGR in these kinds of cell lines(P<0.01).
