云南双江作为新的植烟区,由于之前连年种植甘蔗大量施用氮肥,导致土壤酸化严重,因此须通过增施土壤改良剂改善植烟土壤环境,分析土壤改良剂对双江烤烟K326的生长发育及其品质的影响,筛选出对当地土壤具有较好改良作用的土壤改良剂来改...云南双江作为新的植烟区,由于之前连年种植甘蔗大量施用氮肥,导致土壤酸化严重,因此须通过增施土壤改良剂改善植烟土壤环境,分析土壤改良剂对双江烤烟K326的生长发育及其品质的影响,筛选出对当地土壤具有较好改良作用的土壤改良剂来改善植烟地酸化状况,使其更佳适宜烟株的生长。选择云南双江土壤酸化的植烟地,移栽时在当地常规施肥基础上,增施土壤调理剂硅肥75 kg/667 m 2、钙镁磷肥50 kg/667 m 2和微生物菌肥20 kg/667 m 2等3种土壤改良剂对植烟地进行土壤调节。结果表明,施用硅肥和钙镁磷肥能显著增加叶片数和最大叶宽;增施硅肥后能降低烟叶总糖含量,提高钾离子和氯离子的含量,而且能显著提高中部叶的香气量;施用钙镁磷肥和微生物菌肥提高了烟叶还原糖的含量。施用3种土壤改良剂都有利于烟叶致香成分的积累,其中新植二烯在所有致香成分中最为重要,其含量在中上部叶中总体表现为处理1>处理3>处理2>对照,处理1的中部叶、上部叶新植二烯含量最高,分别为270.42、238.25μg/g,均显著高于对照。因此,在临沧双江烟区常规施肥基础上增施土壤改良剂特别是硅肥,能有效改善烟叶生长状况并提升烟叶品质。展开更多
[Objective] This study was conducted to compare the aroma quality of up-per leaves of K326 flue-cured in different barns, and provide theoretical references for the development and optimization of bulk curing barn and...[Objective] This study was conducted to compare the aroma quality of up-per leaves of K326 flue-cured in different barns, and provide theoretical references for the development and optimization of bulk curing barn and the supporting tech-niques. [Method] The upper leaves of K326 were flue-cured in a commonness barn, a four-shelf bulk curing barn and a three-shelf bulk curing barn separately, and then the contents of aroma components and taste quality indices of the finished leaves were measured and compared. [Result] The contents of total aroma components, chromoplast pigment degradation products, phenylalanine aroma components and neophytadiene showed no significant differences in the leaves flue-cured in four-shelf bulk curing barn and commonness barn, but were significantly or very significantly higher than those in the leaves flue-cured in three-shelf bulk curing barn. The con-tents of carotenoid degradation products, Mail ard reaction products, cembranes aro-ma components and total aroma components （except neophytadiene） showed no significant difference among the three treatments. The results of sensory evaluation revealed that the sensory taste of the leaves flue-cured in four-shelf bulk curing barn was the best, as the finished leaves had rich, delicate aroma, comfortable and clean aftertaste. The leaves flue-cured in commonness barn was better than those flue-cured in three-shelf bulk curing barn in aroma quality, amount and aftertaste. [Conclusion] The four-shelf bulk curing barn had a large volume for tobacco leaf processing, but a similar baking environment to the commonness barn, which is conducive to the formation and accumulation of aroma components, so the finished leaves had similar appearance and quality with those flue-cured in commonness barn. The upper flue-cured leaves in three-shelf bulk curing bran had poorer quality than those flue-cured in the other two barns.展开更多
The growth, chlorophyll composition, photosynthesis, respiration, K uptake and root K+ secretion offlue-cured tobacco as thected by different concentrations of N supplied as urea, NaNO3 and NH4NO3 werestudied under th...The growth, chlorophyll composition, photosynthesis, respiration, K uptake and root K+ secretion offlue-cured tobacco as thected by different concentrations of N supplied as urea, NaNO3 and NH4NO3 werestudied under the experimental condition of sand culture. The results showed that the content of K in thefiue-cured tobacco was not merely related with root vitality and uptake but also closely related with root cellmembrane structure and K+ secretion.展开更多
Drought has severely affected the yield and quality of commercial crops.The BRI1 family plays an important role in plant response to drought stress,and BRL3 gene plays an important role in the study of drought in Arab...Drought has severely affected the yield and quality of commercial crops.The BRI1 family plays an important role in plant response to drought stress,and BRL3 gene plays an important role in the study of drought in Arabidopsis thaliana.In this study,NtBRL3 was constructed as a vector and genetically transformed to obtain‘N.Tobacco K326’overexpression of NtBRL3.The enzyme activities of transgenic tobacco and wild-type tobacco were measured and transcriptome and metabolome analyses were performed.The results showed that the antioxidant enzymes of transgenic tobacco were more active under drought conditions,and 85 significantly differentially metabolites and 106 significantly differentially expressed genes were identified in the metabolome and transcriptome analyses,respectively.Transgenic tobacco NtBRL3ox demonstrated an excessive accumulation of droughtrelated metabolites,sugars such as sucrose and maltotetraose,and amino acids such as proline,compared with WT.We discovered drought-related differential genes in the root transcriptome,among which LOX6,RD22,WSD1,CCD8,and UGT were key genes which play an important role in plant response to drought stress.Our results demonstrate that NtBRL3 overexpression in K326 enhances drought resistance in transgenic tobacco.展开更多
文摘云南双江作为新的植烟区,由于之前连年种植甘蔗大量施用氮肥,导致土壤酸化严重,因此须通过增施土壤改良剂改善植烟土壤环境,分析土壤改良剂对双江烤烟K326的生长发育及其品质的影响,筛选出对当地土壤具有较好改良作用的土壤改良剂来改善植烟地酸化状况,使其更佳适宜烟株的生长。选择云南双江土壤酸化的植烟地,移栽时在当地常规施肥基础上,增施土壤调理剂硅肥75 kg/667 m 2、钙镁磷肥50 kg/667 m 2和微生物菌肥20 kg/667 m 2等3种土壤改良剂对植烟地进行土壤调节。结果表明,施用硅肥和钙镁磷肥能显著增加叶片数和最大叶宽;增施硅肥后能降低烟叶总糖含量,提高钾离子和氯离子的含量,而且能显著提高中部叶的香气量;施用钙镁磷肥和微生物菌肥提高了烟叶还原糖的含量。施用3种土壤改良剂都有利于烟叶致香成分的积累,其中新植二烯在所有致香成分中最为重要,其含量在中上部叶中总体表现为处理1>处理3>处理2>对照,处理1的中部叶、上部叶新植二烯含量最高,分别为270.42、238.25μg/g,均显著高于对照。因此,在临沧双江烟区常规施肥基础上增施土壤改良剂特别是硅肥,能有效改善烟叶生长状况并提升烟叶品质。
文摘[Objective] This study was conducted to compare the aroma quality of up-per leaves of K326 flue-cured in different barns, and provide theoretical references for the development and optimization of bulk curing barn and the supporting tech-niques. [Method] The upper leaves of K326 were flue-cured in a commonness barn, a four-shelf bulk curing barn and a three-shelf bulk curing barn separately, and then the contents of aroma components and taste quality indices of the finished leaves were measured and compared. [Result] The contents of total aroma components, chromoplast pigment degradation products, phenylalanine aroma components and neophytadiene showed no significant differences in the leaves flue-cured in four-shelf bulk curing barn and commonness barn, but were significantly or very significantly higher than those in the leaves flue-cured in three-shelf bulk curing barn. The con-tents of carotenoid degradation products, Mail ard reaction products, cembranes aro-ma components and total aroma components （except neophytadiene） showed no significant difference among the three treatments. The results of sensory evaluation revealed that the sensory taste of the leaves flue-cured in four-shelf bulk curing barn was the best, as the finished leaves had rich, delicate aroma, comfortable and clean aftertaste. The leaves flue-cured in commonness barn was better than those flue-cured in three-shelf bulk curing barn in aroma quality, amount and aftertaste. [Conclusion] The four-shelf bulk curing barn had a large volume for tobacco leaf processing, but a similar baking environment to the commonness barn, which is conducive to the formation and accumulation of aroma components, so the finished leaves had similar appearance and quality with those flue-cured in commonness barn. The upper flue-cured leaves in three-shelf bulk curing bran had poorer quality than those flue-cured in the other two barns.
文摘The growth, chlorophyll composition, photosynthesis, respiration, K uptake and root K+ secretion offlue-cured tobacco as thected by different concentrations of N supplied as urea, NaNO3 and NH4NO3 werestudied under the experimental condition of sand culture. The results showed that the content of K in thefiue-cured tobacco was not merely related with root vitality and uptake but also closely related with root cellmembrane structure and K+ secretion.
基金supported by the Science and Technology Project of Guizhou Tobacco Company(2021XM04)the Creation of“Tobacco T-DNA Activation Insertion Mutant Library and Screening of Important Trait Mutants”Project of Guizhou University Talent Introduction(Guizhou University Hezi[2013]50).
文摘Drought has severely affected the yield and quality of commercial crops.The BRI1 family plays an important role in plant response to drought stress,and BRL3 gene plays an important role in the study of drought in Arabidopsis thaliana.In this study,NtBRL3 was constructed as a vector and genetically transformed to obtain‘N.Tobacco K326’overexpression of NtBRL3.The enzyme activities of transgenic tobacco and wild-type tobacco were measured and transcriptome and metabolome analyses were performed.The results showed that the antioxidant enzymes of transgenic tobacco were more active under drought conditions,and 85 significantly differentially metabolites and 106 significantly differentially expressed genes were identified in the metabolome and transcriptome analyses,respectively.Transgenic tobacco NtBRL3ox demonstrated an excessive accumulation of droughtrelated metabolites,sugars such as sucrose and maltotetraose,and amino acids such as proline,compared with WT.We discovered drought-related differential genes in the root transcriptome,among which LOX6,RD22,WSD1,CCD8,and UGT were key genes which play an important role in plant response to drought stress.Our results demonstrate that NtBRL3 overexpression in K326 enhances drought resistance in transgenic tobacco.
